ATE466948T1 - Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle - Google Patents

Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle

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Publication number
ATE466948T1
ATE466948T1 AT98910289T AT98910289T ATE466948T1 AT E466948 T1 ATE466948 T1 AT E466948T1 AT 98910289 T AT98910289 T AT 98910289T AT 98910289 T AT98910289 T AT 98910289T AT E466948 T1 ATE466948 T1 AT E466948T1
Authority
AT
Austria
Prior art keywords
dna
cell
introducing
nucleic acid
transposon system
Prior art date
Application number
AT98910289T
Other languages
English (en)
Inventor
Perry Hackett
Zoltan Ivics
Zsuzsanna Izsvak
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Application granted granted Critical
Publication of ATE466948T1 publication Critical patent/ATE466948T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
AT98910289T 1997-03-11 1998-03-11 Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle ATE466948T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US4066497P 1997-03-11 1997-03-11
US5386897P 1997-07-28 1997-07-28
US6530397P 1997-11-13 1997-11-13
PCT/US1998/004687 WO1998040510A1 (en) 1997-03-11 1998-03-11 Dna-based transposon system for the introduction of nucleic acid into dna of a cell

Publications (1)

Publication Number Publication Date
ATE466948T1 true ATE466948T1 (de) 2010-05-15

Family

ID=27365765

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98910289T ATE466948T1 (de) 1997-03-11 1998-03-11 Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle

Country Status (10)

Country Link
US (2) US6489458B2 (de)
EP (1) EP0973928B1 (de)
JP (2) JP2002511741A (de)
KR (1) KR20000076157A (de)
AT (1) ATE466948T1 (de)
AU (1) AU745049B2 (de)
CA (1) CA2280997C (de)
DE (1) DE69841648D1 (de)
DK (1) DK0973928T3 (de)
WO (1) WO1998040510A1 (de)

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US7148203B2 (en) 2006-12-12
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