ATE239090T1 - Methode zum generieren von einzelsträngigen dna molekülen - Google Patents

Methode zum generieren von einzelsträngigen dna molekülen

Info

Publication number
ATE239090T1
ATE239090T1 AT94907855T AT94907855T ATE239090T1 AT E239090 T1 ATE239090 T1 AT E239090T1 AT 94907855 T AT94907855 T AT 94907855T AT 94907855 T AT94907855 T AT 94907855T AT E239090 T1 ATE239090 T1 AT E239090T1
Authority
AT
Austria
Prior art keywords
stranded dna
single stranded
dna molecules
generating single
generating
Prior art date
Application number
AT94907855T
Other languages
English (en)
Inventor
Theo Nikiforov
Michael R Knapp
Original Assignee
Orchid Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orchid Biosciences Inc filed Critical Orchid Biosciences Inc
Application granted granted Critical
Publication of ATE239090T1 publication Critical patent/ATE239090T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT94907855T 1993-01-15 1994-01-18 Methode zum generieren von einzelsträngigen dna molekülen ATE239090T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US506193A 1993-01-15 1993-01-15
US08/155,746 US5518900A (en) 1993-01-15 1993-11-23 Method for generating single-stranded DNA molecules
PCT/US1994/000771 WO1994016090A1 (en) 1993-01-15 1994-01-18 Method for generating single-stranded dna molecules

Publications (1)

Publication Number Publication Date
ATE239090T1 true ATE239090T1 (de) 2003-05-15

Family

ID=26673870

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94907855T ATE239090T1 (de) 1993-01-15 1994-01-18 Methode zum generieren von einzelsträngigen dna molekülen

Country Status (8)

Country Link
US (1) US5518900A (de)
EP (1) EP0679190B1 (de)
JP (2) JP3330946B2 (de)
AT (1) ATE239090T1 (de)
AU (1) AU674211B2 (de)
CA (1) CA2153898C (de)
DE (1) DE69432586T2 (de)
WO (1) WO1994016090A1 (de)

Families Citing this family (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
US6015664A (en) * 1995-11-03 2000-01-18 Mcw Research Foundation Multiplex PCR assay using unequal primer concentrations to detect HPIV 1,2,3 and RSV A,B and influenza virus A, B
EP0886681A1 (de) * 1996-03-04 1998-12-30 Genetrace Systems, Inc. Verfahren zur suche von nukleinsäuren mit massenspektroskopie
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
WO1998012355A1 (en) * 1996-09-19 1998-03-26 Genetrace Systems Methods of preparing nucleic acids for mass spectrometric analysis
WO1998020166A2 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
US6635452B1 (en) 1996-12-10 2003-10-21 Sequenom Inc. Releasable nonvolatile mass label molecules
US5876936A (en) * 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
US6046005A (en) * 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
AU730491B2 (en) * 1997-05-16 2001-03-08 Exact Sciences Corporation Electrophoretic analysis of molecules using immobilized probes
US6309829B1 (en) 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6136568A (en) * 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
GB9806253D0 (en) * 1998-03-25 1998-05-20 Tepnel Medical Ltd Amplification of nucleic acids
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6104028A (en) 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
EP1088105A4 (de) * 1998-06-19 2003-06-25 Exact Sciences Corp Detektion nicht-viraler organismen mittels srp-rna
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (de) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus
US7315786B2 (en) 1998-10-16 2008-01-01 Xencor Protein design automation for protein libraries
US20020048772A1 (en) 2000-02-10 2002-04-25 Dahiyat Bassil I. Protein design automation for protein libraries
US6451525B1 (en) * 1998-12-03 2002-09-17 Pe Corporation (Ny) Parallel sequencing method
US6232075B1 (en) * 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
US6743578B1 (en) 1998-12-18 2004-06-01 The Regents Of The University Of California Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
AU2038500A (en) * 1998-12-18 2000-07-12 Regents Of The University Of California, The Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
DE60010058T2 (de) * 1999-02-26 2005-04-28 Exact Sciences Corp., Marlborough Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
WO2000070093A1 (en) * 1999-05-13 2000-11-23 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
EP1250452A1 (de) 1999-12-02 2002-10-23 DNA Sciences, Inc. Verfahren zur bestimmung der variation einzelner nukleotide und genotypisierung
US6762018B1 (en) 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
US7166429B2 (en) 1999-12-29 2007-01-23 Keygene N.V. Method for generating oligonucleotides, in particular for the detection of amplified restriction fragments obtained using AFLP®
WO2001079225A2 (en) * 2000-04-12 2001-10-25 Genaissance Pharmaceuticals, Inc. Haplotypes of the csf1r gene
CA2302827A1 (en) * 2000-04-12 2001-10-12 Michelle Furtado Oligonucleotide and dna de-hybridization on surfaces
US6355433B1 (en) 2000-06-02 2002-03-12 Dna Sciences, Inc. Determination of nucleotide sequence variations through limited primer extension
DE10029914A1 (de) * 2000-06-19 2002-01-03 Epigenomics Ag Verfahren zur hochparallelen Analyse von Polymorphismen
WO2002006469A2 (en) * 2000-07-18 2002-01-24 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
DE10046184A1 (de) * 2000-09-18 2002-04-04 November Ag Molekulare Medizin Verfahren zum Nachweis mindestens einer Nukleinsäuresequenz
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
DE10119203B4 (de) * 2001-04-19 2004-09-16 Rolf Dr. Postina Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung
AU2002303827A1 (en) * 2001-05-25 2002-12-09 Invitrogen Corporation Compositions and methods for extension of nucleic acids
EP1510959A3 (de) * 2001-08-10 2006-07-26 Xencor, Inc. Automatisierte Proteinkonstruktion für Proteinbibliotheken
US7560254B2 (en) * 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
US20050147984A1 (en) * 2001-08-31 2005-07-07 Clondiag Chip Technologies Gmbh Interaction detection on several probe arrays
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
US7125982B1 (en) * 2001-12-05 2006-10-24 Frayne Consultants Microbial production of nuclease resistant DNA, RNA, and oligo mixtures
EP1327682B1 (de) * 2002-01-11 2009-05-13 BioSpring Gesellschaft für Biotechnologie mbH Verfahren zur Herstellung von DNA
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
GB0218089D0 (en) * 2002-08-03 2002-09-11 Epistem Ltd Target DNA
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
JP4643263B2 (ja) * 2002-08-29 2011-03-02 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 対立遺伝子特異的プライマー伸長
JPWO2004061100A1 (ja) * 2002-12-10 2006-05-11 オリンパス株式会社 核酸の変異解析方法および遺伝子の発現解析方法
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP4454631B2 (ja) * 2003-11-15 2010-04-21 ビーエーエスエフ ソシエタス・ヨーロピア 基体に仕上材が設けられた基材
WO2005073409A2 (en) * 2004-01-26 2005-08-11 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
JP2005218385A (ja) * 2004-02-06 2005-08-18 Dnaform:Kk １本鎖ｄｎａを調製するための方法
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US8338578B1 (en) 2004-03-05 2012-12-25 Quest Diagnostics Investments Incorporated Cystic fibrosis gene mutations
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
ATE507305T1 (de) 2004-05-25 2011-05-15 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
EP1623996A1 (de) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
JP2006296279A (ja) * 2005-04-20 2006-11-02 Sony Corp インターカレーターを用いるハイブリダイゼーション検出方法
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007070642A2 (en) * 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US7897747B2 (en) * 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
WO2008092213A1 (en) 2007-02-02 2008-08-07 Genera Biosystems Pty Ltd Generation of nucleic acid molecules
US7881933B2 (en) * 2007-03-23 2011-02-01 Verizon Patent And Licensing Inc. Age determination using speech
WO2009023676A1 (en) * 2007-08-12 2009-02-19 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity
US20090136916A1 (en) * 2007-08-13 2009-05-28 Trustees Of Tufts College Methods and microarrays for detecting enteric viruses
RU2453605C2 (ru) * 2007-09-14 2012-06-20 Игорь Эдуардович Грановский Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк трансгенных растений в пищевых продуктах, способ идентификации трансгенных продуктов, биочип, комбинация олигонуклеотидов (варианты) и набор для осуществления этого способа
EP2489744B1 (de) * 2008-02-04 2014-09-03 Life Technologies Corporation Zusammensetzung und Verfahren zum Sequenzieren von Nukleinsäure
GB0803109D0 (en) * 2008-02-20 2008-03-26 Gene Bridges Gmbh Method of nucleic acid recombination
US8236499B2 (en) 2008-03-28 2012-08-07 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
EP3269824A1 (de) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
CN102124112B (zh) * 2008-09-10 2013-03-20 金斯瑞公司 基于同源重组dna的克隆方法及组合物
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CA2749041C (en) 2008-12-31 2019-09-03 Abbott Point Of Care Inc. Method and device for immunoassay using obligonucleotide conjugates
WO2012058647A1 (en) 2010-10-28 2012-05-03 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
DE102012008375A1 (de) * 2011-04-27 2012-10-31 Genovoxx Gmbh Methoden und Komponenten zur Detektion von Nukleinsäureketten
CA2852949A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
EP2875131B1 (de) 2012-07-18 2018-03-14 Siemens Healthcare Diagnostics Inc. Verfahren zur normalisierung von biologischen proben
CN105026578B (zh) * 2013-03-05 2019-03-15 安捷伦科技有限公司 通过引物延伸合成探针库
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
GB201312995D0 (en) * 2013-07-19 2013-09-04 Atlas Genetics Ltd Methods and kits for specific nucleic acid amplification and detection
US10351899B2 (en) 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
US9546399B2 (en) 2013-11-13 2017-01-17 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015185427A1 (en) * 2014-06-05 2015-12-10 Qiagen Gmbh Optimization of dna amplification reactions
CN107075581B (zh) 2014-08-06 2022-03-18 纽亘技术公司 由靶向测序进行数字测量
GB2531615B (en) 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB201501705D0 (en) 2015-02-02 2015-03-18 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB2530596B (en) 2015-02-02 2016-08-24 Atlas Genetics Ltd Improved blister assembly
US10870879B2 (en) 2015-10-05 2020-12-22 Helmholtz Zentrum Münchendeutsches Forschungszentrum Für Gesundheit Und Umwelt Method for the preparation of bar-coded primer sets
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
EP3692049A4 (de) * 2017-10-04 2021-06-23 Centrillion Technology Holdings Corporation Verfahren und system zur enzymatischen synthese von oligonukleotiden
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
ES2936478T3 (es) 2018-02-05 2023-03-17 Hoffmann La Roche Generación de moldes de ADN circular monocatenario para secuenciación de molécula individual
US20220056498A1 (en) 2019-01-25 2022-02-24 President And Fellows Of Harvard College Compositions and method for synthesizing nucleic acids
AU2020400056A1 (en) 2019-12-12 2022-07-07 President And Fellows Of Harvard College Compositions and methods for light-directed biomolecular barcoding
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
JP2023522958A (ja) 2020-04-22 2023-06-01 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 核酸を検出するための等温法、組成物、キット、およびシステム
EP4225946A4 (de) 2020-10-06 2024-10-30 President And Fellows Of Harvard College Verfahren, zusammensetzungen, kits und systeme für signal-on-nachweis von neutralisierungszielen

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
JP2846018B2 (ja) * 1988-01-21 1999-01-13 ジェネンテク，インコーポレイテッド 核酸配列の増幅および検出
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
ATE173508T1 (de) * 1988-05-20 1998-12-15 Hoffmann La Roche Befestigung von sequenzspezifischen proben
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
FR2650840B1 (fr) * 1989-08-11 1991-11-29 Bertin & Cie Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications

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AU674211B2 (en) 1996-12-12
JPH08505535A (ja) 1996-06-18
JP2002209594A (ja) 2002-07-30
EP0679190A1 (de) 1995-11-02
CA2153898A1 (en) 1994-07-21
AU6126294A (en) 1994-08-15
DE69432586D1 (de) 2003-06-05
JP3330946B2 (ja) 2002-10-07
US5518900A (en) 1996-05-21
EP0679190B1 (de) 2003-05-02
DE69432586T2 (de) 2004-03-25
JP3421664B2 (ja) 2003-06-30
EP0679190A4 (de) 1998-07-01
CA2153898C (en) 2003-10-28
WO1994016090A1 (en) 1994-07-21

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