DE69824716D1 - Methode zur sequenzierung von nukleinsäuren - Google Patents

Methode zur sequenzierung von nukleinsäuren

Info

Publication number
DE69824716D1
DE69824716D1 DE69824716T DE69824716T DE69824716D1 DE 69824716 D1 DE69824716 D1 DE 69824716D1 DE 69824716 T DE69824716 T DE 69824716T DE 69824716 T DE69824716 T DE 69824716T DE 69824716 D1 DE69824716 D1 DE 69824716D1
Authority
DE
Germany
Prior art keywords
nucleic acid
nucleic acids
sequencing nucleic
primers
nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69824716T
Other languages
English (en)
Inventor
H Kawashima
Laurent Farinelli
Pascal Mayer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Solexa Ltd Great Britain
Lynx Therapeutics Inc
Original Assignee
Manteia SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9706529.6A external-priority patent/GB9706529D0/en
Priority claimed from GBGB9713236.9A external-priority patent/GB9713236D0/en
Application filed by Manteia SA filed Critical Manteia SA
Application granted granted Critical
Publication of DE69824716D1 publication Critical patent/DE69824716D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69824716T 1997-04-01 1998-04-01 Methode zur sequenzierung von nukleinsäuren Expired - Lifetime DE69824716D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9706529.6A GB9706529D0 (en) 1997-04-01 1997-04-01 Invention
GBGB9713236.9A GB9713236D0 (en) 1997-06-23 1997-06-23 Invention
PCT/GB1998/000963 WO1998044152A1 (en) 1997-04-01 1998-04-01 Method of nucleic acid sequencing

Publications (1)

Publication Number Publication Date
DE69824716D1 true DE69824716D1 (de) 2004-07-29

Family

ID=26311291

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69824716T Expired - Lifetime DE69824716D1 (de) 1997-04-01 1998-04-01 Methode zur sequenzierung von nukleinsäuren

Country Status (7)

Country Link
US (1) US20020055100A1 (de)
EP (2) EP0975802B1 (de)
JP (1) JP2001517948A (de)
AT (1) ATE269908T1 (de)
AU (1) AU6846798A (de)
DE (1) DE69824716D1 (de)
WO (1) WO1998044152A1 (de)

Families Citing this family (284)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3034626A1 (de) 1997-04-01 2016-06-22 Illumina Cambridge Limited Verfahren zur vervielfältigung von nukleinsäuren
WO1999019341A1 (en) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
AU772281B2 (en) * 1998-12-14 2004-04-22 Li-Cor Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
JP2002539849A (ja) * 1999-03-26 2002-11-26 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ ユニバーサルアレイ
AU773854B2 (en) 1999-04-09 2004-06-10 Keygene N.V. Method for the detection and/or analysis, by means of primer extension techniques, of single nucleotide polymorphisms in restriction fragments, in particular in amplified restriction fragments generated using AFLP
AU4669100A (en) * 1999-04-27 2000-11-10 University Of Chicago, The Nucleotide extension on a microarray of gel-immobilized primers
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
CA2385851A1 (en) * 1999-09-22 2001-03-29 Motorola Inc. Three-dimensional microarray system for parallel genotyping of single nucleotide polymorphisms
US6448010B1 (en) 1999-10-06 2002-09-10 Amersham Pharmacia Biotech, Inc. Method for detecting mutations using arrayed primer extension
WO2001032930A1 (en) * 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
IL138229A (en) * 2000-09-04 2005-03-20 Yissum Res Dev Co Method and system for detecting nucleic acids
US8039602B2 (en) 2000-10-31 2011-10-18 Ryogen Llc Isolated genomic polynucleotide fragments from the p15 region of chromosome 11
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
CA2473308C (en) * 2001-01-24 2013-12-03 Genomic Expression Aps Assay and kit for analyzing gene expression
EP2801624B1 (de) 2001-03-16 2019-03-06 Singular Bio, Inc Arrays und Verfahren zur Verwendung
ATE304610T1 (de) * 2002-04-04 2005-09-15 Biotage Ab Auf primer verlängerung basierendes verfahren, das nukleotide verwendet, die über spaltbare linker markiert sind
AU2003265185A1 (en) * 2002-09-27 2004-04-19 Biotage Ab New sequencing method for sequencing rna molecules
WO2004076683A2 (en) * 2003-02-26 2004-09-10 Callida Genomics, Inc. Random array dna analysis by hybridization
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2789383B1 (de) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molekül-Arrays
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
EP3415641B1 (de) 2004-09-17 2023-11-01 Pacific Biosciences Of California, Inc. Verfahren zur analyse von molekülen
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US8445194B2 (en) 2005-06-15 2013-05-21 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
AT502549B1 (de) 2005-10-07 2007-06-15 Anagnostics Bioanalysis Gmbh Vorrichtung zur analyse von flüssigen proben
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
DK1987159T4 (da) 2006-02-08 2020-11-16 Illumina Cambridge Ltd Fremgangsmåde til sekventering af en polynukleotidtemplate
EP1994180A4 (de) * 2006-02-24 2009-11-25 Callida Genomics Inc Genomsequenzierung auf dna-arrays mit hohem durchsatz
EP2021503A1 (de) * 2006-03-17 2009-02-11 Solexa Ltd. Isothermische methoden zur entstehung von arrays von einzelnen molekülen
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
US20090111705A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20110105366A1 (en) * 2007-06-18 2011-05-05 Illumina, Inc. Microfabrication methods for the optimal patterning of substrates
WO2009032167A1 (en) * 2007-08-29 2009-03-12 Illumina Cambridge Method for sequencing a polynucleotide template
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8298768B2 (en) * 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US7901890B2 (en) 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8617811B2 (en) * 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
WO2011025477A1 (en) 2009-08-25 2011-03-03 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
EP2274449B1 (de) 2008-03-28 2015-12-23 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
US20090270273A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
US20100047876A1 (en) * 2008-08-08 2010-02-25 President And Fellows Of Harvard College Hierarchical assembly of polynucleotides
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
WO2010075188A2 (en) 2008-12-23 2010-07-01 Illumina Inc. Multibase delivery for long reads in sequencing by synthesis protocols
US8772473B2 (en) 2009-03-30 2014-07-08 The Regents Of The University Of California Mostly natural DNA sequencing by synthesis
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
WO2011032040A1 (en) 2009-09-10 2011-03-17 Centrillion Technology Holding Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
WO2011053845A2 (en) 2009-10-30 2011-05-05 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
WO2011106368A2 (en) 2010-02-23 2011-09-01 Illumina, Inc. Amplification methods to minimise sequence specific bias
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
DE202011003570U1 (de) 2010-03-06 2012-01-30 Illumina, Inc. Systeme und Vorrichtungen zum Detektieren optischer Signale aus einer Probe
JP6155419B2 (ja) 2010-03-25 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 検出用の液滴輸送システム
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
EP2632593B1 (de) 2010-10-27 2021-09-29 Illumina, Inc. Durchflusszellen zur biologischen oder chemischen analyse
EP3574990B1 (de) 2010-11-01 2022-04-06 Bio-Rad Laboratories, Inc. System zur formung von emulsionen
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
CN103534360A (zh) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 借助对信号的组合使用进行的多重数字分析
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2012170936A2 (en) 2011-06-09 2012-12-13 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA2841808A1 (en) 2011-07-13 2013-01-17 The Multiple Myeloma Research Foundation, Inc. Methods for data collection and distribution
PT3623481T (pt) 2011-09-23 2021-10-15 Illumina Inc Composições para sequenciação de ácidos nucleicos
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
WO2013102090A1 (en) * 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Isothermal amplification systems and methods
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
CA2867665C (en) 2012-04-03 2022-01-04 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
EP2662692A1 (de) * 2012-05-11 2013-11-13 Alacris Theranostics GmbH Elektrochemische Erkennung von Polymerasereaktionen durch spezifische Metallphosphatkomplexbildung
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
AU2013306373B2 (en) 2012-08-20 2017-09-07 Illumina, Inc. Method and system for fluorescence lifetime based sequencing
EP3901280A1 (de) 2012-10-17 2021-10-27 Spatial Transcriptomics AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
EP2746405B1 (de) 2012-12-23 2015-11-04 HS Diagnomics GmbH Verfahren und Primersätze zur PCR-Hochdurchsatzsequenzierung
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
AU2013382089B2 (en) 2013-03-13 2018-05-10 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
US10557133B2 (en) 2013-03-13 2020-02-11 Illumina, Inc. Methods and compositions for nucleic acid sequencing
WO2014142981A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Enzyme-linked nucleotides
EP4234716A3 (de) 2013-06-25 2023-12-06 Prognosys Biosciences, Inc. Verfahren zur bestimmung der räumlichen muster biologischer ziele in einer probe
KR102266002B1 (ko) 2013-07-01 2021-06-16 일루미나, 인코포레이티드 촉매-무함유 표면 작용화 및 중합체 그라프팅
ES2628485T3 (es) 2013-07-03 2017-08-03 Illumina, Inc. Secuenciación mediante síntesis ortogonal
DK3030645T3 (da) 2013-08-08 2023-01-30 Illumina Inc Fluidsystem til levering af reagenser til en flowcelle
JP6088710B2 (ja) 2013-08-19 2017-03-01 シンギュラー・バイオ・インコーポレイテッド 単分子検出アッセイおよびその使用
EP3038834B1 (de) 2013-08-30 2018-12-12 Illumina, Inc. Manipulation von tröpfchen auf hydrophilen oder variegiert hydrophilen oberflächen
CA2931533C (en) 2013-12-09 2023-08-08 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
WO2015089092A1 (en) 2013-12-10 2015-06-18 Illumina, Inc. Biosensors for biological or chemical analysis and methods of manufacturing the same
CA2928598C (en) 2013-12-19 2022-11-29 M. Shane Bowen Substrates comprising nano-patterning surfaces and methods of preparing thereof
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
US10537889B2 (en) * 2013-12-31 2020-01-21 Illumina, Inc. Addressable flow cell using patterned electrodes
SG10201809312QA (en) 2014-05-16 2018-11-29 Illumina Inc Nucleic acid synthesis techniques
KR102231650B1 (ko) 2014-05-27 2021-03-23 일루미나, 인코포레이티드 베이스 기구 및 제거 가능한 카트리지를 포함하는 생화학적 분석을 위한 시스템들 및 방법들
KR102499300B1 (ko) 2014-06-05 2023-02-10 일루미나, 인코포레이티드 시료 또는 시료 분석 중 적어도 하나를 위한 회전 밸브를 포함하는 시스템들 및 방법들
EP3715468A1 (de) 2014-06-13 2020-09-30 Illumina Cambridge Limited Verfahren und zusammensetzungen zur herstellung von sequenzierungsbibliotheken
WO2016003814A1 (en) 2014-06-30 2016-01-07 Illumina, Inc. Methods and compositions using one-sided transposition
US9982250B2 (en) 2014-08-21 2018-05-29 Illumina Cambridge Limited Reversible surface functionalization
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
JP6802154B2 (ja) 2014-09-18 2020-12-16 イラミーナ インコーポレーテッド 核酸シーケンシングデータを解析するための方法およびシステム
CN106999850B (zh) 2014-10-09 2020-04-07 亿明达股份有限公司 用于分离不混溶的液体以有效地隔离至少一种液体的方法和装置
IL299976B1 (en) 2014-10-17 2024-07-01 Illumina Cambridge Ltd Continuity-preserving transposition
ES2905706T3 (es) 2014-10-31 2022-04-11 Illumina Cambridge Ltd Polímeros y recubrimientos de copolímeros de ADN
CN113528623A (zh) 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
CA3077811C (en) 2015-03-24 2024-02-27 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
CA2982146A1 (en) 2015-04-10 2016-10-13 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
KR102333255B1 (ko) 2015-05-11 2021-12-01 일루미나, 인코포레이티드 치료제의 발견 및 분석을 위한 플랫폼
CA2987397C (en) 2015-05-29 2022-03-01 Illumina, Inc. Sample carrier and assay system for conducting designated reactions
WO2017007753A1 (en) 2015-07-07 2017-01-12 Illumina, Inc. Selective surface patterning via nanoimrinting
CN107835857A (zh) 2015-07-17 2018-03-23 亿明达股份有限公司 用于测序应用的聚合物片层
ES2864677T3 (es) 2015-07-30 2021-10-14 Illumina Inc Desbloqueo ortogonal de nucleótidos
CN108474805A (zh) 2015-08-24 2018-08-31 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
EP3344389B1 (de) 2015-09-02 2020-06-10 Illumina Cambridge Limited Verfahren zur reparatur von defekten in einer hydrophobischen oberfläche eines tröpfchen-aktuators
CN107922929A (zh) 2015-09-09 2018-04-17 凯杰有限公司 聚合酶
DE102015011970B4 (de) 2015-09-09 2017-04-06 Scienion Ag Dispensiergerät und entsprechendes Dispensierverfahren
WO2017087724A1 (en) 2015-11-17 2017-05-26 Omniome, Inc. Methods for determining sequence profiles
CA3008031A1 (en) 2016-01-11 2017-07-20 Illumina Singapore Pte Ltd Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
PT3377226T (pt) 2016-03-28 2021-04-15 Illumina Inc Microarranjos de multiplano
JP6824290B2 (ja) 2016-05-18 2021-02-03 イルミナ インコーポレイテッド パターン化された疎水性表面を用いる自己組織化パターニング
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
US20190348149A1 (en) 2016-11-16 2019-11-14 Illumina, Inc. Validation methods and systems for sequence variant calls
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
US20200002689A1 (en) 2017-02-13 2020-01-02 Qiagen Sciences, Llc Polymerase enzyme from 9°n
WO2018148723A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus abyssi
WO2018148727A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from 9°n
WO2018148724A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from pyrococcus furiosus
WO2018148726A1 (en) 2017-02-13 2018-08-16 Qiagen Waltham Inc. Polymerase enzyme from phage t4
WO2018152162A1 (en) 2017-02-15 2018-08-23 Omniome, Inc. Distinguishing sequences by detecting polymerase dissociation
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CA3072136A1 (en) 2017-08-15 2019-02-21 Omniome, Inc. Scanning apparatus and methods useful for detection of chemical and biological analytes
EP3622520A1 (de) * 2017-10-16 2020-03-18 Illumina, Inc. Auf tiefenlernen basierende techniken für das training von tiefen neuronalen faltungsnetzwerken
WO2019079202A1 (en) 2017-10-16 2019-04-25 Illumina, Inc. ABERRANT CONNECTION DETECTION USING CONVOLUTION NEURAL NETWORKS (CNN)
WO2020081122A1 (en) 2018-10-15 2020-04-23 Illumina, Inc. Deep learning-based techniques for pre-training deep convolutional neural networks
EP3697932A1 (de) 2017-10-19 2020-08-26 Omniome, Inc. Gleichzeitige hintergrundreduktion und komplexstabilisierung in bindungsassayarbeitsflüssen
CN110268383A (zh) 2017-11-13 2019-09-20 多发性骨髓瘤研究基金会公司 综合、分子、组学、免疫疗法、代谢、表观遗传学和临床数据库
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
SG11201911784PA (en) 2018-01-08 2020-01-30 Illumina Inc Systems and devices for high-throughput sequencing with semiconductor-based detection
NZ759659A (en) 2018-01-15 2022-07-01 Illumina Inc Deep learning-based variant classifier
KR20210111345A (ko) 2018-03-22 2021-09-10 일루미나, 인코포레이티드 Rna 및 dna로부터의 핵산 라이브러리의 제조
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
EP3782158A1 (de) 2018-04-19 2021-02-24 Omniome, Inc. Verbesserung der genauigkeit von basenzuordnungen in nukleinsäuresequenzierungsverfahren
JP7554117B2 (ja) 2018-04-26 2024-09-19 パシフィック・バイオサイエンシズ・オブ・カリフォルニア・インク． 核酸－ヌクレオチド－ポリメラーゼ複合体を安定化するための方法及び組成物
DK3794012T3 (da) 2018-05-15 2024-01-08 Illumina Inc Sammensætninger og fremgangsmåder til kemisk spaltning og afbeskyttelse af overfladebundne oligonukleotider
EP3802874A1 (de) 2018-05-31 2021-04-14 Omniome, Inc. Erhöhtes signal-rausch-verhältnis bei der sequenzierung von nukleinsäuren
US12073922B2 (en) 2018-07-11 2024-08-27 Illumina, Inc. Deep learning-based framework for identifying sequence patterns that cause sequence-specific errors (SSEs)
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113767175A (zh) 2018-08-28 2021-12-07 10X基因组学股份有限公司 增加空间阵列分辨率
US20240011086A1 (en) 2018-11-15 2024-01-11 Omniome, Inc. Electronic detection of nucleic acid structure
CN211311477U (zh) 2018-12-04 2020-08-21 欧姆尼欧美公司 测序系统
US20220049294A1 (en) 2018-12-10 2022-02-17 10X Genomics, Inc. Imaging system hardware
WO2020132103A1 (en) 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020167574A1 (en) 2019-02-14 2020-08-20 Omniome, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (zh) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 用于检测化学和生物分析物的扫描装置和方法
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
CA3104851A1 (en) 2019-05-16 2020-11-19 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
WO2020252186A1 (en) 2019-06-11 2020-12-17 Omniome, Inc. Calibrated focus sensing
WO2021011803A1 (en) 2019-07-16 2021-01-21 Omniome, Inc. Synthetic nucleic acids having non-natural structures
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
CN114728996B (zh) 2019-09-10 2022-11-29 加利福尼亚太平洋生物科学股份有限公司 核苷酸的可逆修饰
EP4045683A1 (de) 2019-10-18 2022-08-24 Omniome, Inc. Verfahren und zusammensetzungen zum kappen von nukleinsäuren
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
ES2946357T3 (es) 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
CN115243792A (zh) 2020-02-04 2022-10-25 加利福尼亚太平洋生物科学股份有限公司 流通池及其制造和使用方法
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
CN115136244A (zh) 2020-02-20 2022-09-30 因美纳有限公司 基于人工智能的多对多碱基判读
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
DK3969618T3 (da) 2020-03-09 2023-10-16 Illumina Inc Fremgangsmåder til sekventering af polynukleotider
JP2023517156A (ja) 2020-03-09 2023-04-24 イルミナ，インコーポレイテッド 核酸配列決定における改善
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4146822A1 (de) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Zusammensetzungen und verfahren zur modifizierung von polymerase-nukleinsäurekomplexen
EP4414459A3 (de) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4162074B1 (de) 2020-06-08 2024-04-24 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
MX2022016492A (es) 2020-06-22 2023-03-06 Illumina Cambridge Ltd Nucleosidos y nucleotidos con grupo de bloqueo de acetal 3'.
EP4172362B1 (de) 2020-06-25 2024-09-18 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (de) 2020-12-21 2023-01-25 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
AU2022245985A1 (en) 2021-03-22 2023-09-21 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
US20220336054A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures
CN117916390A (zh) 2021-05-20 2024-04-19 伊鲁米纳公司 用于边合成边测序的组合物和方法
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
WO2023278608A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Self-learned base caller, trained using oligo sequences
US20230027409A1 (en) 2021-07-13 2023-01-26 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
KR20240031968A (ko) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 염기 호출에 대한 보간 및 적응을 갖는 강도 추출
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
CN117813391A (zh) 2021-07-23 2024-04-02 因美纳有限公司 制备用于dna测序的基底表面的方法
AU2022319125A1 (en) 2021-07-28 2024-01-18 Illumina, Inc. Quality score calibration of basecalling systems
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
CA3234961A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
AU2022409487A1 (en) 2021-12-16 2024-01-18 Illumina, Inc. Hybrid clustering
CN117813390A (zh) 2021-12-16 2024-04-02 因美纳有限公司 用于表面结合的多核苷酸的金属定向裂解的方法
US20240279733A1 (en) 2021-12-17 2024-08-22 Illumina, Inc. Orthogonal hybridization
CN117940577A (zh) 2021-12-20 2024-04-26 因美纳有限公司 用于化学裂解表面结合的多核苷酸的高碘酸盐组合物和方法
CN117813399A (zh) 2021-12-20 2024-04-02 因美纳有限公司 用于化学裂解表面结合的多核苷酸的高碘酸盐组合物和方法
WO2023141154A1 (en) 2022-01-20 2023-07-27 Illumina Cambridge Limited Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
EP4341425A2 (de) 2022-03-15 2024-03-27 Illumina, Inc. Gleichzeitige sequenzierung von vorwärts- und rückwärtskomplementketten auf verketteten polynukleotiden zur methylierungsdetektion
WO2023175013A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods for preparing signals for concurrent sequencing
US20230313294A1 (en) 2022-03-30 2023-10-05 Illumina Cambridge Limited Methods for chemical cleavage of surface-bound polynucleotides
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
US20240124916A1 (en) 2022-09-16 2024-04-18 Illumina Cambridge Limited Nanoparticle with polynucleotide binding site and method of making thereof
WO2024061799A1 (en) 2022-09-19 2024-03-28 Illumina, Inc. Deformable polymers comprising immobilised primers
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates
WO2024137765A1 (en) 2022-12-22 2024-06-27 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
US20240247311A1 (en) 2022-12-22 2024-07-25 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
WO2024145154A1 (en) 2022-12-27 2024-07-04 Illumina, Inc. Methods of sequencing using 3´ allyl blocked nucleotides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
DE4141178A1 (de) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog Verfahren zur sequenzierung von nukleinsaeuren
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
JPH07203998A (ja) * 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
WO1996027025A1 (en) * 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
EP0830460A1 (de) * 1995-04-11 1998-03-25 Trustees Of Boston University Festphasensequenzierung von biopolymeren
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
AU5797696A (en) * 1995-05-19 1996-11-29 Ely Michael Rabani Parallel multiplex polynucleotide sequencing

Also Published As

Publication number Publication date
WO1998044152A1 (en) 1998-10-08
EP0975802B1 (de) 2004-06-23
US20020055100A1 (en) 2002-05-09
ATE269908T1 (de) 2004-07-15
AU6846798A (en) 1998-10-22
JP2001517948A (ja) 2001-10-09
EP1498494A3 (de) 2007-06-20
EP1498494A2 (de) 2005-01-19
EP0975802A1 (de) 2000-02-02

Similar Documents

Publication Publication Date Title
ATE269908T1 (de) Methode zur sequenzierung von nukleinsäuren
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
DE69627698T2 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
BR9407712A (pt) Métodos e composiçoes para sequenciamento eficaz de ácidos nuclêicos
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
EP2267164A3 (de) Sequenzanalyse von Nukleinsäuren
PT92640A (pt) Method for making synthetic oligonucleotides which bind specifically to target sites on duplex dna molecules by forming a colinear triplex the synthetic oligonucleotides and methods of use
WO1998039485A3 (en) Compositions and methods for analysis of nucleic acids
ATE232880T1 (de) Neue nucleotidanaloga
ATE286124T1 (de) Verfahren zur herstellung von polynukleotidvarianten
NZ511415A (en) Multi-loci genomic analysis by a method of improved cycle sequencing
DE59410068D1 (de) Verfahren zur spezifischen Klonierung von Nukleinsäuren
WO1998037231A3 (de) Markierung von nukleinsäuren mit speziellen probengemischen
DE59912683D1 (de) Verfahren und Zusammensetzungen zur Isolierung und Reinigung von Nukleinsäuren

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: SOLEXA LTD., SAFFRON WALDEN, ESSEX, GB

Owner name: LYNX THERAPEUTICS, INC., HAYWARD, CALIF., US

8332 No legal effect for de