ATE286124T1 - Verfahren zur herstellung von polynukleotidvarianten - Google Patents

Verfahren zur herstellung von polynukleotidvarianten

Info

Publication number
ATE286124T1
ATE286124T1 AT02790188T AT02790188T ATE286124T1 AT E286124 T1 ATE286124 T1 AT E286124T1 AT 02790188 T AT02790188 T AT 02790188T AT 02790188 T AT02790188 T AT 02790188T AT E286124 T1 ATE286124 T1 AT E286124T1
Authority
AT
Austria
Prior art keywords
pcr
polynucleotides
mutated
polynucleotide variants
series
Prior art date
Application number
AT02790188T
Other languages
English (en)
Inventor
Roelof Ary Lans Bovenberg
Richard Kerkman
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26076965&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE286124(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE286124T1 publication Critical patent/ATE286124T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT02790188T 2001-07-23 2002-07-23 Verfahren zur herstellung von polynukleotidvarianten ATE286124T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01202821 2001-07-23
EP01203457 2001-09-11
PCT/EP2002/008225 WO2003010183A2 (en) 2001-07-23 2002-07-23 Process for preparing variant polynucleotides

Publications (1)

Publication Number Publication Date
ATE286124T1 true ATE286124T1 (de) 2005-01-15

Family

ID=26076965

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02790188T ATE286124T1 (de) 2001-07-23 2002-07-23 Verfahren zur herstellung von polynukleotidvarianten

Country Status (7)

Country Link
US (1) US7402383B2 (de)
EP (1) EP1409667B2 (de)
AT (1) ATE286124T1 (de)
AU (1) AU2002333269A1 (de)
DE (1) DE60202478T3 (de)
DK (1) DK1409667T4 (de)
WO (1) WO2003010183A2 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE286124T1 (de) 2001-07-23 2005-01-15 Dsm Ip Assets Bv Verfahren zur herstellung von polynukleotidvarianten
EP2123772A1 (de) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-Lactamantibiotikum produzierende Stämme
EP2123767A1 (de) 2008-05-20 2009-11-25 DSM IP Assets B.V. Herstellung von Epsilon-Caprolactam mittels Lysinzyklisierung
EP2123768A1 (de) 2008-05-20 2009-11-25 DSM IP Assets B.V. Herstellung von (Z)-6,7-Dihydro-1H-azepin-2(5H)-on
EP2130918A1 (de) * 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
EP2392649A3 (de) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA-produzierende Stämme
EP2406384A2 (de) 2009-03-11 2012-01-18 DSM IP Assets B.V. Herstellung von alpha-ketopimelinsäure
WO2010122141A1 (en) 2009-04-24 2010-10-28 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
AU2011214327B2 (en) 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
CA2803982A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
PL2588492T3 (pl) 2010-06-29 2016-09-30 Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania
WO2012000891A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
US9163223B2 (en) 2010-06-29 2015-10-20 Dsm Ip Assets B.V. Polypeptide having acetyl xylan esterase activity and uses thereof
EP2588603B1 (de) 2010-06-29 2017-03-22 DSM IP Assets B.V. Zellulaseerweiterndes polypeptid und verwendungen davon
AU2011273685B2 (en) 2010-06-29 2014-12-11 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
WO2012031910A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Method for preparing alpha-ketopimelic acid by c1-elongation
EA201400836A8 (ru) 2012-01-23 2016-03-31 ДСМ АйПи АССЕТС Б.В. Получение дитерпена
EP3686280B1 (de) 2013-02-04 2022-06-22 DSM IP Assets B.V. Kohlenhydratabbauendes polypeptid und verwendungen davon
US10689681B2 (en) 2013-05-31 2020-06-23 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2015007748A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Diterpene production
EP3536697A1 (de) 2013-07-31 2019-09-11 DSM IP Assets B.V. Rückgewinnung von steviolglycosiden
US10829748B2 (en) 2016-06-10 2020-11-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
WO2019213624A1 (en) * 2018-05-04 2019-11-07 The Regents Of The University Of California Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids
US20230080079A1 (en) 2018-05-18 2023-03-16 Dsm Ip Assets B.V. Mutant lipase and use thereof
WO2019219904A2 (en) 2018-05-18 2019-11-21 Dsm Ip Assets B.V. Mutant lipase and use thereof
CN109750032A (zh) * 2019-02-28 2019-05-14 尚科生物医药（上海）有限公司 一种构建基因多点突变和进化的方法
EP3766982A1 (de) 2019-07-18 2021-01-20 Delft Advanced Biofuels B.V. Integriertes system zur biokatalytischen herstellung und rückgewinnung einer organischen substanz
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
EP4698673A1 (de) 2023-04-20 2026-02-25 DSM IP Assets B.V. Stämme und verfahren zur herstellung von mogrosiden
WO2025144785A2 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
WO1997007205A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Method for preparing polypeptide variants
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6242222B1 (en) * 1996-06-07 2001-06-05 Massachusetts Institute Of Technology Programmed sequential mutagenesis
EP1717322B1 (de) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution ganzer Zellen durch rekursive Sequenzrekombination
DK1015575T3 (da) 1997-03-18 2010-08-23 Novozymes As Shuffling af heterologe DNA-sekvenser
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6767701B1 (en) * 1998-10-26 2004-07-27 Novozymes A/S Methods of constructing and screening a DNA library of interest in filamentous fungal cells
JP2002534965A (ja) * 1999-01-19 2002-10-22 マキシジェン， インコーポレイテッド 所望の特徴を有する文字ストリング、ポリヌクレオチドおよびポリペプチドを作製するための方法
WO2001029212A1 (en) 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
ATE286124T1 (de) 2001-07-23 2005-01-15 Dsm Ip Assets Bv Verfahren zur herstellung von polynukleotidvarianten
WO2003010311A2 (en) * 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides

Also Published As

Publication number Publication date
EP1409667B2 (de) 2009-06-10
DK1409667T4 (da) 2009-09-07
US20050130141A1 (en) 2005-06-16
DK1409667T3 (da) 2005-04-25
EP1409667A2 (de) 2004-04-21
US7402383B2 (en) 2008-07-22
AU2002333269A1 (en) 2003-02-17
EP1409667B1 (de) 2004-12-29
DE60202478T3 (de) 2010-01-07
DE60202478D1 (de) 2005-02-03
DE60202478T2 (de) 2005-12-29
WO2003010183A3 (en) 2003-11-13
WO2003010183A2 (en) 2003-02-06

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