DK1409667T3 - Fremgangsmåde til fremstilling af polynucleotidvarianter - Google Patents

Fremgangsmåde til fremstilling af polynucleotidvarianter

Info

Publication number
DK1409667T3
DK1409667T3 DK02790188T DK02790188T DK1409667T3 DK 1409667 T3 DK1409667 T3 DK 1409667T3 DK 02790188 T DK02790188 T DK 02790188T DK 02790188 T DK02790188 T DK 02790188T DK 1409667 T3 DK1409667 T3 DK 1409667T3
Authority
DK
Denmark
Prior art keywords
pcr
polynucleotides
preparation
mutated
polynucleotide variants
Prior art date
Application number
DK02790188T
Other languages
English (en)
Other versions
DK1409667T4 (da
Inventor
Richard Kerkman
Roelof Ary Lans Bovenberg
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26076965&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1409667(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of DK1409667T3 publication Critical patent/DK1409667T3/da
Application granted granted Critical
Publication of DK1409667T4 publication Critical patent/DK1409667T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DK02790188T 2001-07-23 2002-07-23 Fremgangsm der til at fremstilling varierende polynucleotider DK1409667T4 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01202821 2001-07-23
EP01203457 2001-09-11
PCT/EP2002/008225 WO2003010183A2 (en) 2001-07-23 2002-07-23 Process for preparing variant polynucleotides

Publications (2)

Publication Number Publication Date
DK1409667T3 true DK1409667T3 (da) 2005-04-25
DK1409667T4 DK1409667T4 (da) 2009-09-07

Family

ID=26076965

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02790188T DK1409667T4 (da) 2001-07-23 2002-07-23 Fremgangsm der til at fremstilling varierende polynucleotider

Country Status (7)

Country Link
US (1) US7402383B2 (da)
EP (1) EP1409667B2 (da)
AT (1) ATE286124T1 (da)
AU (1) AU2002333269A1 (da)
DE (1) DE60202478T3 (da)
DK (1) DK1409667T4 (da)
WO (1) WO2003010183A2 (da)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE286124T1 (de) 2001-07-23 2005-01-15 Dsm Ip Assets Bv Verfahren zur herstellung von polynukleotidvarianten
EP2123772A1 (en) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-lactam antibiotic producing strains
EP2123767A1 (en) 2008-05-20 2009-11-25 DSM IP Assets B.V. Preparation of epsilon-caprolactam via lysine cyclisation
EP2123768A1 (en) 2008-05-20 2009-11-25 DSM IP Assets B.V. Preparation of (Z)-6,7-dihydro-1H-azepin-2(5H)-one
EP2130918A1 (de) * 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
EP2392649A3 (en) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA producing strains
US20120156737A1 (en) 2009-03-11 2012-06-21 Dsm Ip Assets B.V. Preparation of alpha-ketopimelic acid
CA2758396C (en) 2009-04-24 2018-06-12 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
EP2534243A1 (en) 2010-02-11 2012-12-19 DSM IP Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
CN103391999B (zh) 2010-06-29 2016-06-22 帝斯曼知识产权资产管理有限公司 具有乙酰基木聚糖酯酶活性的多肽及其用途
CA2801824A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
MY160897A (en) 2010-06-29 2017-03-31 Dsm Ip Assets Bv Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
CA2803982A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
CA2801647A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
DK2588492T3 (da) 2010-06-29 2016-06-27 Dsm Ip Assets Bv Polypeptid med beta-glucosidaseaktivitet og anvendelser deraf
WO2012031910A2 (en) 2010-09-10 2012-03-15 Dsm Ip Assets B.V. Method for preparing alpha-ketopimelic acid by c1-elongation
US20150031868A1 (en) 2012-01-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production
ES2802807T3 (es) 2013-02-04 2021-01-21 Dsm Ip Assets Bv Polipéptido degradador de carbohidratos y sus usos
EP3502264A3 (en) 2013-05-31 2019-11-06 DSM IP Assets B.V. Microorganisms for diterpene production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2015007748A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Diterpene production
BR112016002092B1 (pt) 2013-07-31 2020-12-15 Dsm Ip Assets B.V. Recuperação de glicosídeos de esteviol
US10829748B2 (en) 2016-06-10 2020-11-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
WO2019213624A1 (en) * 2018-05-04 2019-11-07 The Regents Of The University Of California Spiked primers for enrichment of pathogen nucleic acids among background of nucleic acids
CA3100611A1 (en) 2018-05-18 2019-11-21 Dsm Ip Assets B.V. Mutant lipase and use thereof
US20230250406A1 (en) 2018-05-18 2023-08-10 Dsm Ip Assets B.V. Mutant lipase and use thereof
CN109750032A (zh) * 2019-02-28 2019-05-14 尚科生物医药（上海）有限公司 一种构建基因多点突变和进化的方法
EP3766982A1 (en) 2019-07-18 2021-01-20 Delft Advanced Biofuels B.V. Integrated system for biocatalytically producing and recovering an organic substance
EP4232568A1 (en) 2020-10-22 2023-08-30 DSM IP Assets B.V. Microorganisms for diterpene production

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK0843725T3 (da) 1995-08-11 2002-08-12 Novozymes As Fremgangmåde til fremstilling af polypeptidvarianter
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1997046670A1 (en) * 1996-06-07 1997-12-11 Massachusetts Institute Of Technology Programmed sequential mutagenesis
DE69835360T2 (de) 1997-01-17 2007-08-16 Maxygen, Inc., Redwood City EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION
CA2284097A1 (en) 1997-03-18 1998-09-24 Novo Nordisk A/S Shuffling of heterologous dna sequences
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6767701B1 (en) * 1998-10-26 2004-07-27 Novozymes A/S Methods of constructing and screening a DNA library of interest in filamentous fungal cells
EP1108783A3 (en) 1999-01-19 2001-09-05 Maxygen, Inc. Oligonucleotide-mediated nucleic acid recombination
WO2001029212A1 (en) * 1999-10-19 2001-04-26 Enchira Biotechnology Corporation Methods for chimeragenesis of whole genomes or large polynucleotides
US20050130140A1 (en) 2001-07-23 2005-06-16 Bovenberg Roelof A.L. Process for preparing variant polynucleotides
ATE286124T1 (de) 2001-07-23 2005-01-15 Dsm Ip Assets Bv Verfahren zur herstellung von polynukleotidvarianten

Also Published As

Publication number Publication date
ATE286124T1 (de) 2005-01-15
DE60202478D1 (de) 2005-02-03
EP1409667B1 (en) 2004-12-29
WO2003010183A2 (en) 2003-02-06
EP1409667B2 (en) 2009-06-10
EP1409667A2 (en) 2004-04-21
DE60202478T2 (de) 2005-12-29
US20050130141A1 (en) 2005-06-16
US7402383B2 (en) 2008-07-22
AU2002333269A1 (en) 2003-02-17
WO2003010183A3 (en) 2003-11-13
DE60202478T3 (de) 2010-01-07
DK1409667T4 (da) 2009-09-07

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