ATE331040T1 - Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) - Google Patents
Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)Info
- Publication number
- ATE331040T1 ATE331040T1 AT98917984T AT98917984T ATE331040T1 AT E331040 T1 ATE331040 T1 AT E331040T1 AT 98917984 T AT98917984 T AT 98917984T AT 98917984 T AT98917984 T AT 98917984T AT E331040 T1 ATE331040 T1 AT E331040T1
- Authority
- AT
- Austria
- Prior art keywords
- reverse transcriptase
- amplification
- pcr
- molecules
- methods
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4262997P | 1997-04-03 | 1997-04-03 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE331040T1 true ATE331040T1 (de) | 2006-07-15 |
Family
ID=21922934
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT98917984T ATE331040T1 (de) | 1997-04-03 | 1998-04-03 | Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) |
AT06012490T ATE486961T1 (de) | 1997-04-03 | 1998-04-03 | Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06012490T ATE486961T1 (de) | 1997-04-03 | 1998-04-03 | Verfahren und produkte zur reversen transkription gekoppelt mit polymerasekettenreaktion (rt-pcr) |
Country Status (8)
Country | Link |
---|---|
US (6) | US6495350B1 (de) |
EP (3) | EP0975806B1 (de) |
AT (2) | ATE331040T1 (de) |
AU (1) | AU7100198A (de) |
DE (2) | DE69835008T2 (de) |
DK (1) | DK1705253T3 (de) |
ES (1) | ES2355739T3 (de) |
WO (1) | WO1998044161A1 (de) |
Families Citing this family (41)
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US6593120B1 (en) | 1994-04-01 | 2003-07-15 | Gen-Probe Incorporated | Recombinant DNA encoding a reverse transcriptase derived from moloney murine leukemia virus |
ES2355739T3 (es) * | 1997-04-03 | 2011-03-30 | Life Technologies Corporation | Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr). |
WO1998047912A1 (en) | 1997-04-22 | 1998-10-29 | Life Technologies, Inc. | Methods for the production of aslv reverse transcriptases composed of multiple subunits |
US7122304B2 (en) | 1997-05-12 | 2006-10-17 | Whatman, Inc. | Methods for the storage and synthesis of nucleic acids using a solid support |
US20040185493A1 (en) * | 1997-05-12 | 2004-09-23 | Invitrogen Corporation | Methods for production and purification of nucleic acid molecules |
US7521178B1 (en) * | 1998-04-23 | 2009-04-21 | Takara Bio Inc. | Method for synthesizing DNA |
US7074556B2 (en) * | 1999-03-02 | 2006-07-11 | Invitrogen Corporation | cDNA synthesis improvements |
US6300069B1 (en) * | 1999-05-03 | 2001-10-09 | Qiagen Gmbh | Generation and amplification of nucleic acids from ribonucleic acids |
NZ515673A (en) * | 1999-05-21 | 2004-03-26 | Invitrogen Corp | Compositions and methods for labeling of nucleic acid molecules |
CA2460546A1 (en) * | 2001-09-14 | 2003-03-27 | Invitrogen Corporation | Dna polymerases and mutants thereof |
US20040152072A1 (en) * | 2002-07-30 | 2004-08-05 | Invitrogen Corporation | Reverse transcription |
DE60329853D1 (de) * | 2002-09-03 | 2009-12-10 | Quanta Biosciences Inc | Verbesserte zusammensetzungen und verfahren für die cdna-synthese |
US20040157213A1 (en) * | 2003-01-27 | 2004-08-12 | Kennell John C. | Fungal reverse transcriptases with enhanced capabilities |
US20040259115A1 (en) * | 2003-01-29 | 2004-12-23 | Schuster David M. | Methods and compositions for RNA detection and quantitation |
US7727752B2 (en) | 2003-07-29 | 2010-06-01 | Life Technologies Corporation | Kinase and phosphatase assays |
JP4866248B2 (ja) | 2004-01-23 | 2012-02-01 | バイオメリュー・インコーポレイテッド | 核酸増幅性能改良のための新規ヌクレオチド混合物 |
EP1807512A4 (de) * | 2004-04-06 | 2008-12-31 | Stratagene California | Kompositionen und verfahren zur rücktranskription |
US7566532B1 (en) | 2004-07-02 | 2009-07-28 | Quest Diagnostics Investments Incorporated | Methods for detecting retroviruses |
US20060024725A1 (en) | 2004-07-30 | 2006-02-02 | Robert Hussa | Oncofetal fibronectin as a marker for pregnancy-related indications |
WO2006050499A2 (en) * | 2004-11-03 | 2006-05-11 | Third Wave Technologies, Inc. | Single step detection assay |
EP1989318B1 (de) * | 2006-01-06 | 2014-07-30 | Agilent Technologies, Inc. | Reaktionspufferzusammensetzung für die nukleinsäurereplikation mit gepackten dna-polymerasen |
EP2759307B1 (de) | 2006-03-29 | 2016-11-09 | Merial Limited | Impfstoff gegen Streptokokken |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
EP3029146B1 (de) * | 2007-11-27 | 2018-08-01 | Bio-rad Laboratories, Inc. | Reduzierte hemmung einer einstufigen rt-pcr |
JP5279339B2 (ja) * | 2008-05-16 | 2013-09-04 | タカラバイオ株式会社 | 逆転写反応用組成物 |
KR101419980B1 (ko) | 2008-03-15 | 2014-07-15 | 홀로직, 인크. | 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법 |
DK2582839T3 (en) * | 2010-06-21 | 2016-08-29 | Life Technologies Corp | Compositions, methods and kits for nucleic acid synthesis and amplification by RT |
EP2582850B1 (de) | 2010-06-21 | 2016-11-09 | Life Technologies Corporation | Zusammensetzungen, kits und verfahren zur synthese und/oder zum nachweis von nukleinsäuren |
WO2012054727A1 (en) | 2010-10-22 | 2012-04-26 | Bio-Rad Laboratories, Inc. | Reverse transcriptase mixtures with improved storage stability |
WO2012150317A1 (en) * | 2011-05-05 | 2012-11-08 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Linear dna amplification |
WO2012170907A2 (en) * | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
EP4249603A3 (de) | 2011-06-08 | 2024-01-03 | Life Technologies Corporation | Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen |
EP2559764A1 (de) | 2011-08-17 | 2013-02-20 | Qiagen GmbH | Zusammensetzung und Verfahren für reverse Transkriptase-Polymerase-Kettenreaktion (RT-PCR )mit einem anionischen Polymer |
WO2014063051A2 (en) | 2012-10-18 | 2014-04-24 | Idexx Laboratories, Inc. | Nucleic acid amplification controls and kits and methods of use thereof |
CN106458885B (zh) | 2013-10-25 | 2019-12-10 | 生命技术公司 | 用于聚合酶链式反应系统的新颖化合物及其应用 |
CN109476695A (zh) | 2016-06-27 | 2019-03-15 | 丹娜法伯癌症研究院 | 用于测定rna翻译速率的方法 |
WO2018136404A1 (en) * | 2017-01-19 | 2018-07-26 | Asuragen, Inc. | Methods of rna amplification |
WO2019118652A1 (en) | 2017-12-12 | 2019-06-20 | Essenlix Corporation | Sample manipulation and assay with rapid temperature change |
US11851702B2 (en) * | 2020-03-23 | 2023-12-26 | The Broad Institute, Inc. | Rapid diagnostics |
WO2022018685A1 (en) | 2020-07-22 | 2022-01-27 | King Abdullah University Of Science And Technology | Compositions and methods for one-step nucleic acid amplification and diagnostic methods based thereon |
WO2023278704A1 (en) | 2021-06-30 | 2023-01-05 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for enrichment of nucleic acids using light-mediated cross-linking |
Family Cites Families (32)
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US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5561058A (en) * | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
US5407800A (en) | 1986-08-22 | 1995-04-18 | Hoffmann-La Roche Inc. | Reverse transcription with Thermus thermophilus polymerase |
US5618702A (en) | 1988-08-19 | 1997-04-08 | City Of Hope | PCR amplification of mRNA |
US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
EP0894860B1 (de) | 1990-09-28 | 2012-12-12 | F. Hoffmann-La Roche AG | Thermostabile DNA Polymerasen und deren Deletionsmutanten |
USRE39031E1 (en) | 1992-04-29 | 2006-03-21 | Biomerieux | RNA amplification method requiring only one manipulation step |
FR2690691B1 (fr) | 1992-04-29 | 1999-02-12 | Bio Merieux | Procede d'amplification d'arn necessitant une seule etape de manipulation. |
US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
US5514551A (en) * | 1994-10-14 | 1996-05-07 | Gen-Probe Incorporated | Compositions for the detection of Chlamydia trachomatis |
JPH08131171A (ja) | 1994-11-11 | 1996-05-28 | Asahi Chem Ind Co Ltd | 逆転写酵素の安定化組成物 |
EP0736608A1 (de) * | 1995-04-08 | 1996-10-09 | Roche Diagnostics GmbH | Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA |
US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
US6060240A (en) * | 1996-12-13 | 2000-05-09 | Arcaris, Inc. | Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom |
ES2355739T3 (es) * | 1997-04-03 | 2011-03-30 | Life Technologies Corporation | Composiciones y métodos para la reacción en cadena de la polimerasa de transcriptasa inversa (rt-pcr). |
WO1998047912A1 (en) | 1997-04-22 | 1998-10-29 | Life Technologies, Inc. | Methods for the production of aslv reverse transcriptases composed of multiple subunits |
US7074556B2 (en) | 1999-03-02 | 2006-07-11 | Invitrogen Corporation | cDNA synthesis improvements |
US6630333B1 (en) | 1999-03-23 | 2003-10-07 | Invitrogen Corporation | Substantially pure reverse transriptases and methods of production thereof |
US7078208B2 (en) | 2000-05-26 | 2006-07-18 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
US8278083B2 (en) | 2004-03-22 | 2012-10-02 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Inactivated influenza virus compositions |
-
1998
- 1998-04-03 ES ES06012490T patent/ES2355739T3/es not_active Expired - Lifetime
- 1998-04-03 DK DK06012490.6T patent/DK1705253T3/da active
- 1998-04-03 WO PCT/US1998/006581 patent/WO1998044161A1/en active IP Right Grant
- 1998-04-03 EP EP98917984A patent/EP0975806B1/de not_active Expired - Lifetime
- 1998-04-03 EP EP06012490A patent/EP1705253B1/de not_active Expired - Lifetime
- 1998-04-03 DE DE69835008T patent/DE69835008T2/de not_active Expired - Lifetime
- 1998-04-03 AT AT98917984T patent/ATE331040T1/de not_active IP Right Cessation
- 1998-04-03 AT AT06012490T patent/ATE486961T1/de not_active IP Right Cessation
- 1998-04-03 DE DE69841990T patent/DE69841990D1/de not_active Expired - Lifetime
- 1998-04-03 AU AU71001/98A patent/AU7100198A/en not_active Abandoned
- 1998-04-03 EP EP10178653.1A patent/EP2354251B1/de not_active Expired - Lifetime
-
1999
- 1999-12-23 US US09/472,066 patent/US6495350B1/en not_active Expired - Lifetime
-
2002
- 2002-08-21 US US10/224,334 patent/US6767724B2/en not_active Expired - Lifetime
-
2004
- 2004-06-07 US US10/861,469 patent/US7883871B2/en not_active Expired - Fee Related
-
2010
- 2010-12-29 US US12/981,240 patent/US8372604B2/en not_active Expired - Fee Related
-
2013
- 2013-02-11 US US13/764,754 patent/US9556466B2/en not_active Expired - Fee Related
-
2014
- 2014-09-26 US US14/498,827 patent/US9873901B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP1705253A3 (de) | 2006-12-27 |
ES2355739T3 (es) | 2011-03-30 |
DK1705253T3 (da) | 2011-02-21 |
US6767724B2 (en) | 2004-07-27 |
EP0975806A4 (de) | 2004-10-06 |
DE69835008T2 (de) | 2007-01-11 |
US20130164788A1 (en) | 2013-06-27 |
US8372604B2 (en) | 2013-02-12 |
EP1705253A2 (de) | 2006-09-27 |
WO1998044161A1 (en) | 1998-10-08 |
EP2354251B1 (de) | 2014-10-15 |
US20040219595A1 (en) | 2004-11-04 |
US20110217735A1 (en) | 2011-09-08 |
EP0975806A1 (de) | 2000-02-02 |
DE69835008D1 (de) | 2006-08-03 |
EP2354251A1 (de) | 2011-08-10 |
EP1705253B1 (de) | 2010-11-03 |
US9873901B2 (en) | 2018-01-23 |
DE69841990D1 (de) | 2010-12-16 |
US20030113712A1 (en) | 2003-06-19 |
ATE486961T1 (de) | 2010-11-15 |
EP0975806B1 (de) | 2006-06-21 |
US9556466B2 (en) | 2017-01-31 |
US7883871B2 (en) | 2011-02-08 |
AU7100198A (en) | 1998-10-22 |
US20150017688A1 (en) | 2015-01-15 |
US6495350B1 (en) | 2002-12-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |