DE60329853D1 - Verbesserte zusammensetzungen und verfahren für die cdna-synthese - Google Patents

Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Info

Publication number
DE60329853D1
DE60329853D1 DE60329853T DE60329853T DE60329853D1 DE 60329853 D1 DE60329853 D1 DE 60329853D1 DE 60329853 T DE60329853 T DE 60329853T DE 60329853 T DE60329853 T DE 60329853T DE 60329853 D1 DE60329853 D1 DE 60329853D1
Authority
DE
Germany
Prior art keywords
cdna
methods
compositions
cdna synthesis
kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60329853T
Other languages
English (en)
Inventor
Ayoub Rashtchian
David M Schuster
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quanta BioSciences Inc
Original Assignee
Quanta BioSciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31978445&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60329853(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Quanta BioSciences Inc filed Critical Quanta BioSciences Inc
Application granted granted Critical
Publication of DE60329853D1 publication Critical patent/DE60329853D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
DE60329853T 2002-09-03 2003-09-03 Verbesserte zusammensetzungen und verfahren für die cdna-synthese Expired - Lifetime DE60329853D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40724802P 2002-09-03 2002-09-03
PCT/US2003/027520 WO2004021986A2 (en) 2002-09-03 2003-09-03 Improved compositions and methods for cdna synthesis

Publications (1)

Publication Number Publication Date
DE60329853D1 true DE60329853D1 (de) 2009-12-10

Family

ID=31978445

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60329853T Expired - Lifetime DE60329853D1 (de) 2002-09-03 2003-09-03 Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Country Status (10)

Country Link
US (5) US7638612B2 (de)
EP (3) EP3067429A1 (de)
AT (1) ATE447040T1 (de)
AU (1) AU2003268397A1 (de)
CA (1) CA2497420C (de)
DE (1) DE60329853D1 (de)
DK (1) DK2143803T3 (de)
ES (1) ES2579803T3 (de)
PT (1) PT2143803T (de)
WO (1) WO2004021986A2 (de)

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CA2818890C (en) * 2010-11-23 2022-07-12 Krisani Biosciences (P) Ltd Method and system for prognosis and treatment of diseases using portfolio of genes
CN104364392B (zh) 2012-02-27 2018-05-25 赛卢拉研究公司 用于分子计数的组合物和试剂盒
US20140199749A1 (en) * 2013-01-15 2014-07-17 Jun Euihum Lee Compositions and Methods for cDNA Synthesis
EP3828284A1 (de) * 2013-03-15 2021-06-02 Abbott Molecular Inc. Einstufiges verfahren zur aufreinigung von nukleinsäuren
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
ES2548299B2 (es) * 2014-03-13 2016-05-13 Universidad De Málaga Firma de microARN como indicador del riesgo de recurrencia temprana en pacientes con cáncer de mama
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20160097085A1 (en) * 2014-10-03 2016-04-07 Jun Euihum Lee Compositions and Methods for cDNA Synthesis
EP3262192B1 (de) 2015-02-27 2020-09-16 Becton, Dickinson and Company Räumlich adressierbare molekulare barcodierung
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
EP4086357A1 (de) * 2015-08-28 2022-11-09 Illumina, Inc. Nukleinsäuresequenzanalyse aus einzelnen zellen
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
EP3635135A1 (de) 2017-06-05 2020-04-15 Becton, Dickinson and Company Probenindizierung für einzelzellen
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
JP2022511398A (ja) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
JP2022506546A (ja) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
CN113574178A (zh) 2019-01-23 2021-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
EP3924506A1 (de) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybride gezielte und gesamttranskriptomamplifikation
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4090763A1 (de) 2020-01-13 2022-11-23 Becton Dickinson and Company Verfahren und zusammensetzungen zur quantifizierung von proteinen und rna
CN111518669A (zh) * 2020-05-07 2020-08-11 安徽科技学院 一种核酸检测微流控芯片及其应用
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN118339306A (zh) * 2021-12-02 2024-07-12 生物辐射实验室股份有限公司 Dna:rna双链体断裂的用途

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ATE447040T1 (de) 2002-09-03 2009-11-15 Quanta Biosciences Inc Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Also Published As

Publication number Publication date
EP3067429A1 (de) 2016-09-14
US7638612B2 (en) 2009-12-29
EP2143803A1 (de) 2010-01-13
EP2143803B1 (de) 2016-04-13
ES2579803T3 (es) 2016-08-16
EP3067429A9 (de) 2016-10-26
US20110151520A1 (en) 2011-06-23
US9988660B2 (en) 2018-06-05
EP1534850B1 (de) 2009-10-28
DK2143803T3 (en) 2016-08-01
US20040110201A1 (en) 2004-06-10
US20130244286A1 (en) 2013-09-19
EP1534850A4 (de) 2007-01-03
AU2003268397A1 (en) 2004-03-29
PT2143803T (pt) 2016-07-14
US20160046975A1 (en) 2016-02-18
CA2497420C (en) 2016-04-19
US20060068436A1 (en) 2006-03-30
US7470515B2 (en) 2008-12-30
WO2004021986A2 (en) 2004-03-18
US8420324B2 (en) 2013-04-16
EP1534850A2 (de) 2005-06-01
WO2004021986A3 (en) 2004-06-24
AU2003268397A8 (en) 2004-03-29
ATE447040T1 (de) 2009-11-15
CA2497420A1 (en) 2004-03-18

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