ATE447040T1 - Verbesserte zusammensetzungen und verfahren für die cdna-synthese - Google Patents

Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Info

Publication number
ATE447040T1
ATE447040T1 AT03749359T AT03749359T ATE447040T1 AT E447040 T1 ATE447040 T1 AT E447040T1 AT 03749359 T AT03749359 T AT 03749359T AT 03749359 T AT03749359 T AT 03749359T AT E447040 T1 ATE447040 T1 AT E447040T1
Authority
AT
Austria
Prior art keywords
cdna
methods
compositions
cdna synthesis
kits
Prior art date
Application number
AT03749359T
Other languages
English (en)
Inventor
Ayoub Rashtchian
David Schuster
Original Assignee
Quanta Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=31978445&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE447040(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Quanta Biosciences Inc filed Critical Quanta Biosciences Inc
Application granted granted Critical
Publication of ATE447040T1 publication Critical patent/ATE447040T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
AT03749359T 2002-09-03 2003-09-03 Verbesserte zusammensetzungen und verfahren für die cdna-synthese ATE447040T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40724802P 2002-09-03 2002-09-03
PCT/US2003/027520 WO2004021986A2 (en) 2002-09-03 2003-09-03 Improved compositions and methods for cdna synthesis

Publications (1)

Publication Number Publication Date
ATE447040T1 true ATE447040T1 (de) 2009-11-15

Family

ID=31978445

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03749359T ATE447040T1 (de) 2002-09-03 2003-09-03 Verbesserte zusammensetzungen und verfahren für die cdna-synthese

Country Status (10)

Country Link
US (5) US7638612B2 (de)
EP (3) EP2143803B1 (de)
AT (1) ATE447040T1 (de)
AU (1) AU2003268397A1 (de)
CA (1) CA2497420C (de)
DE (1) DE60329853D1 (de)
DK (1) DK2143803T3 (de)
ES (1) ES2579803T3 (de)
PT (1) PT2143803T (de)
WO (1) WO2004021986A2 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006736A1 (en) 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
EP2143803B1 (de) * 2002-09-03 2016-04-13 Quanta Biosciences, Inc. Verbesserte Zusammensetzungen und Verfahren zur cDNA-Synthese
WO2005040396A2 (en) * 2003-10-16 2005-05-06 Genomic Health, Inc. qRT-PCR ASSAY SYSTEM FOR GENE EXPRESSION PROFILING
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012054727A1 (en) 2010-10-22 2012-04-26 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
JP5731167B2 (ja) * 2010-10-29 2015-06-10 シスメックス株式会社 一本鎖cDNAの合成方法、マイクロアレイ用試料の調製方法および核酸の検出方法
SG190867A1 (en) 2010-11-23 2013-07-31 Krisani Biosciences P Ltd Method and system for prognosis and treatment of diseases using portfolio of genes
EP2820158B1 (de) 2012-02-27 2018-01-10 Cellular Research, Inc. Zusammensetzungen und kits zur molekularen zählung
US20140199749A1 (en) * 2013-01-15 2014-07-17 Jun Euihum Lee Compositions and Methods for cDNA Synthesis
JP6431522B2 (ja) * 2013-03-15 2018-11-28 アボツト・モレキユラー・インコーポレイテツド 核酸の精製のための1工程法
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
ES2548299B2 (es) * 2014-03-13 2016-05-13 Universidad De Málaga Firma de microARN como indicador del riesgo de recurrencia temprana en pacientes con cáncer de mama
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20160097085A1 (en) * 2014-10-03 2016-04-07 Jun Euihum Lee Compositions and Methods for cDNA Synthesis
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3341494B1 (de) * 2015-08-28 2024-08-07 Illumina, Inc. Analyse von nukleinsäuresequenzen aus einzelnen zellen
EP3347465B1 (de) 2015-09-11 2019-06-26 Cellular Research, Inc. Verfahren und zusammensetzung zur standardisierung von nukleinsäure bibliothek
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CA3034924A1 (en) 2016-09-26 2018-03-29 Cellular Research, Inc. Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US10676779B2 (en) 2017-06-05 2020-06-09 Becton, Dickinson And Company Sample indexing for single cells
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
EP3861134B1 (de) 2018-10-01 2024-09-04 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
EP3894552A1 (de) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
CN113454234A (zh) 2019-02-14 2021-09-28 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
CN111518669A (zh) * 2020-05-07 2020-08-11 安徽科技学院 一种核酸检测微流控芯片及其应用
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116635533A (zh) 2020-11-20 2023-08-22 贝克顿迪金森公司 高表达的蛋白和低表达的蛋白的谱分析
CN118339306A (zh) * 2021-12-02 2024-07-12 生物辐射实验室股份有限公司 Dna:rna双链体断裂的用途

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US5244797B1 (en) 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
US5747296A (en) 1992-05-11 1998-05-05 Corvas International, Inc. Method of detecting neutophil inhibitory factor mimics
US6214542B1 (en) * 1992-10-20 2001-04-10 The United States Of America As Represented By The Department Of Health And Human Services Quantification of indicators of fibrosis
DE4336266A1 (de) * 1993-10-23 1995-04-27 Boehringer Mannheim Gmbh Stabilisierte flüssige Mischungen für die Markierung von Nukleinsäuren
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
DE69739357D1 (de) * 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
WO1998006736A1 (en) 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
ATE331040T1 (de) * 1997-04-03 2006-07-15 Invitrogen Corp Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr)
US6120999A (en) * 1997-07-10 2000-09-19 Human Genome Sciences, Inc. Histidine kinase two-component in Candida albicans
US6087489A (en) 1998-06-02 2000-07-11 Isis Pharmaceuticals Inc. Antisense oligonucleotide modulation of human thymidylate synthase expression
US6406891B1 (en) * 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6300069B1 (en) * 1999-05-03 2001-10-09 Qiagen Gmbh Generation and amplification of nucleic acids from ribonucleic acids
US6083727A (en) 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
ATE481499T1 (de) * 1999-09-24 2010-10-15 Ambion Inc Cocktail von nukleaseinhibitoren
US6300073B1 (en) 1999-10-01 2001-10-09 Clontech Laboratories, Inc. One step RT-PCR methods, enzyme mixes and kits for use in practicing the same
US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US6521411B2 (en) * 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
AU2002236524A1 (en) * 2000-11-28 2002-06-11 Rosetta Inpharmatics, Inc. In vitro transcription method for rna amplification
CN1384203A (zh) * 2001-04-30 2002-12-11 香港科技创业股份有限公司 具有高度延伸专一性的dna低温循环延伸反应方法
EP2143803B1 (de) * 2002-09-03 2016-04-13 Quanta Biosciences, Inc. Verbesserte Zusammensetzungen und Verfahren zur cDNA-Synthese

Also Published As

Publication number Publication date
US7638612B2 (en) 2009-12-29
US8420324B2 (en) 2013-04-16
EP3067429A1 (de) 2016-09-14
EP2143803A1 (de) 2010-01-13
WO2004021986A3 (en) 2004-06-24
US20040110201A1 (en) 2004-06-10
US20160046975A1 (en) 2016-02-18
ES2579803T3 (es) 2016-08-16
EP1534850A2 (de) 2005-06-01
WO2004021986A2 (en) 2004-03-18
EP1534850B1 (de) 2009-10-28
CA2497420C (en) 2016-04-19
US20060068436A1 (en) 2006-03-30
US9988660B2 (en) 2018-06-05
AU2003268397A8 (en) 2004-03-29
AU2003268397A1 (en) 2004-03-29
US20130244286A1 (en) 2013-09-19
PT2143803T (pt) 2016-07-14
DE60329853D1 (de) 2009-12-10
EP2143803B1 (de) 2016-04-13
DK2143803T3 (en) 2016-08-01
CA2497420A1 (en) 2004-03-18
US20110151520A1 (en) 2011-06-23
EP1534850A4 (de) 2007-01-03
US7470515B2 (en) 2008-12-30
EP3067429A9 (de) 2016-10-26

Similar Documents

Publication Publication Date Title
ATE447040T1 (de) Verbesserte zusammensetzungen und verfahren für die cdna-synthese
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
WO2007041342A3 (en) Labeled nucleotide analogs and uses therefor
DE69839668D1 (de) Ein in-vitro-verfahren zur herstellung einer dna-bibliothek
WO2003008624A3 (en) Nucleic acid amplification using nicking agents
WO2006135684A3 (en) Methods and kits for sense rna synthesis
WO2005014035A3 (en) Use of galactose oxidase for selective chemical conjugation of protractor molecules to proteins of therapeutic interest
DE60034396D1 (de) Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure
WO2003020739A3 (en) Novel lna compositions and uses thereof
WO2003020979A8 (en) Methods for preparing nucleic acid samples
EP1671998A3 (de) Verfahren zur Herstellung von Komponenten mit Indolocarbazol-Gruppen
WO2005030984A3 (en) Method for small volume nucleic acid synthesis
EP2314596A3 (de) DNA-Polymerase-mischungen und mutante DNA-polymerasen
WO2001025483A3 (en) Compositions for dna amplification, synthesis, and mutagenesis
WO2004022770A3 (en) Compositions and methods for synthesizing nucleic acids
WO2005118866A9 (en) COMPOSITIONS AND METHODS FOR SYNTHESIZING cDNA
WO2008016562A3 (en) Nucleic acid amplification using a reversibly modified oligonucleotide
WO2006081623A8 (en) Double-stranded oligonucleotides and uses therefor
WO2007028833A3 (de) Verfahren zur aktivierung einer nukleinsäure für eine polymerase-reaktion
WO2002086155A3 (en) Method for the preferential nucleic acid amplification of selected nucleic acid regions
WO2006119419A3 (en) Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction
WO2004068111A3 (en) Methods and compositions for rna detection and quantitation
WO2007035685A3 (en) Compositions and methods for purifying nucleic acids
DE602005025424D1 (de) Neuartiges Nukleotidgemisch für eine verbesserte Nukleinsäurenamplifikationsleistung
EP1624058A3 (de) Verfahren und Zusammensetzungen zur Durchführung Matrizen-abhängiger Nukleinsäure-Primer-Verlängerungsreaktionen, wodurch ein Produkt mit reduzierter Komplexität hergestellt wird

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties