ATE447040T1 - Verbesserte zusammensetzungen und verfahren für die cdna-synthese - Google Patents
Verbesserte zusammensetzungen und verfahren für die cdna-syntheseInfo
- Publication number
- ATE447040T1 ATE447040T1 AT03749359T AT03749359T ATE447040T1 AT E447040 T1 ATE447040 T1 AT E447040T1 AT 03749359 T AT03749359 T AT 03749359T AT 03749359 T AT03749359 T AT 03749359T AT E447040 T1 ATE447040 T1 AT E447040T1
- Authority
- AT
- Austria
- Prior art keywords
- cdna
- methods
- compositions
- cdna synthesis
- kits
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Production Of Liquid Hydrocarbon Mixture For Refining Petroleum (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US40724802P | 2002-09-03 | 2002-09-03 | |
PCT/US2003/027520 WO2004021986A2 (en) | 2002-09-03 | 2003-09-03 | Improved compositions and methods for cdna synthesis |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE447040T1 true ATE447040T1 (de) | 2009-11-15 |
Family
ID=31978445
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03749359T ATE447040T1 (de) | 2002-09-03 | 2003-09-03 | Verbesserte zusammensetzungen und verfahren für die cdna-synthese |
Country Status (10)
Country | Link |
---|---|
US (5) | US7638612B2 (de) |
EP (3) | EP2143803B1 (de) |
AT (1) | ATE447040T1 (de) |
AU (1) | AU2003268397A1 (de) |
CA (1) | CA2497420C (de) |
DE (1) | DE60329853D1 (de) |
DK (1) | DK2143803T3 (de) |
ES (1) | ES2579803T3 (de) |
PT (1) | PT2143803T (de) |
WO (1) | WO2004021986A2 (de) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998006736A1 (en) | 1996-08-14 | 1998-02-19 | Life Technologies, Inc. | Stable compositions for nucleic acid amplification and sequencing |
EP2143803B1 (de) * | 2002-09-03 | 2016-04-13 | Quanta Biosciences, Inc. | Verbesserte Zusammensetzungen und Verfahren zur cDNA-Synthese |
WO2005040396A2 (en) * | 2003-10-16 | 2005-05-06 | Genomic Health, Inc. | qRT-PCR ASSAY SYSTEM FOR GENE EXPRESSION PROFILING |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
WO2012054727A1 (en) | 2010-10-22 | 2012-04-26 | Bio-Rad Laboratories, Inc. | Reverse transcriptase mixtures with improved storage stability |
JP5731167B2 (ja) * | 2010-10-29 | 2015-06-10 | シスメックス株式会社 | 一本鎖cDNAの合成方法、マイクロアレイ用試料の調製方法および核酸の検出方法 |
SG190867A1 (en) | 2010-11-23 | 2013-07-31 | Krisani Biosciences P Ltd | Method and system for prognosis and treatment of diseases using portfolio of genes |
EP2820158B1 (de) | 2012-02-27 | 2018-01-10 | Cellular Research, Inc. | Zusammensetzungen und kits zur molekularen zählung |
US20140199749A1 (en) * | 2013-01-15 | 2014-07-17 | Jun Euihum Lee | Compositions and Methods for cDNA Synthesis |
JP6431522B2 (ja) * | 2013-03-15 | 2018-11-28 | アボツト・モレキユラー・インコーポレイテツド | 核酸の精製のための1工程法 |
GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
ES2548299B2 (es) * | 2014-03-13 | 2016-05-13 | Universidad De Málaga | Firma de microARN como indicador del riesgo de recurrencia temprana en pacientes con cáncer de mama |
US10975371B2 (en) * | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
US20160097085A1 (en) * | 2014-10-03 | 2016-04-07 | Jun Euihum Lee | Compositions and Methods for cDNA Synthesis |
CN107208158B (zh) | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
JP7508191B2 (ja) | 2015-03-30 | 2024-07-01 | ベクトン・ディキンソン・アンド・カンパニー | コンビナトリアルバーコーディングのための方法および組成物 |
WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
EP3341494B1 (de) * | 2015-08-28 | 2024-08-07 | Illumina, Inc. | Analyse von nukleinsäuresequenzen aus einzelnen zellen |
EP3347465B1 (de) | 2015-09-11 | 2019-06-26 | Cellular Research, Inc. | Verfahren und zusammensetzung zur standardisierung von nukleinsäure bibliothek |
US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
CA3034924A1 (en) | 2016-09-26 | 2018-03-29 | Cellular Research, Inc. | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
US10676779B2 (en) | 2017-06-05 | 2020-06-09 | Becton, Dickinson And Company | Sample indexing for single cells |
US11365409B2 (en) | 2018-05-03 | 2022-06-21 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
EP3861134B1 (de) | 2018-10-01 | 2024-09-04 | Becton, Dickinson and Company | Bestimmung von 5'-transkriptsequenzen |
US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
EP3894552A1 (de) | 2018-12-13 | 2021-10-20 | Becton, Dickinson and Company | Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen |
US11661631B2 (en) | 2019-01-23 | 2023-05-30 | Becton, Dickinson And Company | Oligonucleotides associated with antibodies |
CN113454234A (zh) | 2019-02-14 | 2021-09-28 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
WO2021016239A1 (en) | 2019-07-22 | 2021-01-28 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
CN111518669A (zh) * | 2020-05-07 | 2020-08-11 | 安徽科技学院 | 一种核酸检测微流控芯片及其应用 |
CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司(Us) | 用于免疫组库谱分析的引物 |
US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
CN116635533A (zh) | 2020-11-20 | 2023-08-22 | 贝克顿迪金森公司 | 高表达的蛋白和低表达的蛋白的谱分析 |
CN118339306A (zh) * | 2021-12-02 | 2024-07-12 | 生物辐射实验室股份有限公司 | Dna:rna双链体断裂的用途 |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
US5561058A (en) * | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
US5747296A (en) | 1992-05-11 | 1998-05-05 | Corvas International, Inc. | Method of detecting neutophil inhibitory factor mimics |
US6214542B1 (en) * | 1992-10-20 | 2001-04-10 | The United States Of America As Represented By The Department Of Health And Human Services | Quantification of indicators of fibrosis |
DE4336266A1 (de) * | 1993-10-23 | 1995-04-27 | Boehringer Mannheim Gmbh | Stabilisierte flüssige Mischungen für die Markierung von Nukleinsäuren |
US5556771A (en) | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
DE69739357D1 (de) * | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
WO1998006736A1 (en) | 1996-08-14 | 1998-02-19 | Life Technologies, Inc. | Stable compositions for nucleic acid amplification and sequencing |
ATE331040T1 (de) * | 1997-04-03 | 2006-07-15 | Invitrogen Corp | Zusammensetzungen und verfahren der reversen transkriptase-polymerasekettenreaktion (rt-pcr) |
US6120999A (en) * | 1997-07-10 | 2000-09-19 | Human Genome Sciences, Inc. | Histidine kinase two-component in Candida albicans |
US6087489A (en) | 1998-06-02 | 2000-07-11 | Isis Pharmaceuticals Inc. | Antisense oligonucleotide modulation of human thymidylate synthase expression |
US6406891B1 (en) * | 1998-09-28 | 2002-06-18 | Board Of Regents, The University Of Texas System | Dual RT procedure for cDNA synthesis |
US6300069B1 (en) * | 1999-05-03 | 2001-10-09 | Qiagen Gmbh | Generation and amplification of nucleic acids from ribonucleic acids |
US6083727A (en) | 1999-06-30 | 2000-07-04 | Incyte Pharmaceuticals Inc. | Methods and compositions for producing 5' enriched cDNA libraries |
ATE481499T1 (de) * | 1999-09-24 | 2010-10-15 | Ambion Inc | Cocktail von nukleaseinhibitoren |
US6300073B1 (en) | 1999-10-01 | 2001-10-09 | Clontech Laboratories, Inc. | One step RT-PCR methods, enzyme mixes and kits for use in practicing the same |
US7078208B2 (en) * | 2000-05-26 | 2006-07-18 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
US6521411B2 (en) * | 2000-09-28 | 2003-02-18 | Transgenomic, Inc. | Method and system for the preparation of cDNA |
AU2002236524A1 (en) * | 2000-11-28 | 2002-06-11 | Rosetta Inpharmatics, Inc. | In vitro transcription method for rna amplification |
CN1384203A (zh) * | 2001-04-30 | 2002-12-11 | 香港科技创业股份有限公司 | 具有高度延伸专一性的dna低温循环延伸反应方法 |
EP2143803B1 (de) * | 2002-09-03 | 2016-04-13 | Quanta Biosciences, Inc. | Verbesserte Zusammensetzungen und Verfahren zur cDNA-Synthese |
-
2003
- 2003-09-03 EP EP09013546.8A patent/EP2143803B1/de not_active Revoked
- 2003-09-03 PT PT90135468T patent/PT2143803T/pt unknown
- 2003-09-03 DK DK09013546.8T patent/DK2143803T3/en active
- 2003-09-03 DE DE60329853T patent/DE60329853D1/de not_active Expired - Lifetime
- 2003-09-03 CA CA2497420A patent/CA2497420C/en not_active Expired - Lifetime
- 2003-09-03 US US10/653,416 patent/US7638612B2/en not_active Expired - Lifetime
- 2003-09-03 EP EP03749359A patent/EP1534850B1/de not_active Expired - Lifetime
- 2003-09-03 AU AU2003268397A patent/AU2003268397A1/en not_active Abandoned
- 2003-09-03 WO PCT/US2003/027520 patent/WO2004021986A2/en not_active Application Discontinuation
- 2003-09-03 EP EP16164558.5A patent/EP3067429A1/de not_active Withdrawn
- 2003-09-03 AT AT03749359T patent/ATE447040T1/de not_active IP Right Cessation
- 2003-09-03 ES ES09013546.8T patent/ES2579803T3/es not_active Expired - Lifetime
-
2005
- 2005-09-29 US US11/237,922 patent/US7470515B2/en not_active Expired - Lifetime
-
2009
- 2009-12-08 US US12/632,802 patent/US8420324B2/en not_active Expired - Lifetime
-
2013
- 2013-03-04 US US13/783,780 patent/US20130244286A1/en not_active Abandoned
-
2015
- 2015-10-08 US US14/878,751 patent/US9988660B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US7638612B2 (en) | 2009-12-29 |
US8420324B2 (en) | 2013-04-16 |
EP3067429A1 (de) | 2016-09-14 |
EP2143803A1 (de) | 2010-01-13 |
WO2004021986A3 (en) | 2004-06-24 |
US20040110201A1 (en) | 2004-06-10 |
US20160046975A1 (en) | 2016-02-18 |
ES2579803T3 (es) | 2016-08-16 |
EP1534850A2 (de) | 2005-06-01 |
WO2004021986A2 (en) | 2004-03-18 |
EP1534850B1 (de) | 2009-10-28 |
CA2497420C (en) | 2016-04-19 |
US20060068436A1 (en) | 2006-03-30 |
US9988660B2 (en) | 2018-06-05 |
AU2003268397A8 (en) | 2004-03-29 |
AU2003268397A1 (en) | 2004-03-29 |
US20130244286A1 (en) | 2013-09-19 |
PT2143803T (pt) | 2016-07-14 |
DE60329853D1 (de) | 2009-12-10 |
EP2143803B1 (de) | 2016-04-13 |
DK2143803T3 (en) | 2016-08-01 |
CA2497420A1 (en) | 2004-03-18 |
US20110151520A1 (en) | 2011-06-23 |
EP1534850A4 (de) | 2007-01-03 |
US7470515B2 (en) | 2008-12-30 |
EP3067429A9 (de) | 2016-10-26 |
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Legal Events
Date | Code | Title | Description |
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |