DE69739357D1 - Überwachung der hybridisierung während pcr - Google Patents

Überwachung der hybridisierung während pcr

Info

Publication number
DE69739357D1
DE69739357D1 DE69739357T DE69739357T DE69739357D1 DE 69739357 D1 DE69739357 D1 DE 69739357D1 DE 69739357 T DE69739357 T DE 69739357T DE 69739357 T DE69739357 T DE 69739357T DE 69739357 D1 DE69739357 D1 DE 69739357D1
Authority
DE
Germany
Prior art keywords
nucleic acid
target nucleic
acid sequence
biological sample
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739357T
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English (en)
Inventor
Carl T Wittwer
Kirk M Ririe
Randy P Rasmussen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Utah Research Foundation UURF
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University of Utah Research Foundation UURF
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Application filed by University of Utah Research Foundation UURF filed Critical University of Utah Research Foundation UURF
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Publication of DE69739357D1 publication Critical patent/DE69739357D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Fats And Perfumes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE69739357T 1996-06-04 1997-06-04 Überwachung der hybridisierung während pcr Expired - Lifetime DE69739357D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US65899396A 1996-06-04 1996-06-04
US81826797A 1997-03-17 1997-03-17
PCT/US1997/010008 WO1997046714A1 (en) 1996-06-04 1997-06-04 Monitoring hybridization during pcr

Publications (1)

Publication Number Publication Date
DE69739357D1 true DE69739357D1 (de) 2009-05-28

Family

ID=27097737

Family Applications (3)

Application Number Title Priority Date Filing Date
DE69735313T Expired - Lifetime DE69735313T2 (de) 1996-06-04 1997-06-04 Fluoreszenz-Donor-Akzeptor Paar
DE69739357T Expired - Lifetime DE69739357D1 (de) 1996-06-04 1997-06-04 Überwachung der hybridisierung während pcr
DE69733282T Expired - Lifetime DE69733282T2 (de) 1996-06-04 1997-06-04 Überwachung der Hybridisierung während PCR

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69735313T Expired - Lifetime DE69735313T2 (de) 1996-06-04 1997-06-04 Fluoreszenz-Donor-Akzeptor Paar

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69733282T Expired - Lifetime DE69733282T2 (de) 1996-06-04 1997-06-04 Überwachung der Hybridisierung während PCR

Country Status (12)

Country Link
US (9) US6174670B1 (de)
EP (3) EP1033411B1 (de)
JP (4) JP4540754B2 (de)
AT (3) ATE295427T1 (de)
AU (1) AU726501B2 (de)
CA (3) CA2658290C (de)
DE (3) DE69735313T2 (de)
DK (2) DK0912766T4 (de)
ES (2) ES2243393T3 (de)
NZ (2) NZ333136A (de)
PT (2) PT912766E (de)
WO (1) WO1997046714A1 (de)

Families Citing this family (779)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070269799A9 (en) * 1994-06-22 2007-11-22 Zhang David Y Nucleic acid amplification methods
CA2658290C (en) 1996-06-04 2012-04-10 University Of Utah Research Foundation Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna
WO1998036096A1 (en) * 1997-02-14 1998-08-20 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
US6518017B1 (en) * 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
US20030165888A1 (en) * 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
EP2034031A1 (de) * 1997-10-28 2009-03-11 Los Alamos National Security, LLC Bestimmung der Identität von DNA-Polymorphismen mittels Durchflusszytometrie
CA2308368C (en) * 1997-11-04 2009-01-20 Roche Diagnostics Gmbh Specific and sensitive nucleic acid detection method
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
WO1999040219A1 (en) * 1998-02-05 1999-08-12 Bavarian Nordic Research Institute A/S Quantification by inhibition of amplification
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6270973B1 (en) * 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
DE19811729C2 (de) * 1998-03-18 2000-05-18 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Nachweis einer Nukleotidsequenz
DE19811731A1 (de) * 1998-03-18 1999-09-23 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleotidsequenz
US6361942B1 (en) 1998-03-24 2002-03-26 Boston Probes, Inc. Method, kits and compositions pertaining to detection complexes
US6132968A (en) * 1998-05-13 2000-10-17 University Of Alberta Methods for quantitating low level modifications of nucleotide sequences
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
US6780617B2 (en) 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
GB9815799D0 (en) * 1998-07-21 1998-09-16 Pharmagene Lab Limited Quantitative analysis of gene expression
GB9819417D0 (en) 1998-09-07 1998-10-28 Secr Defence Reaction method
GB9819418D0 (en) * 1998-09-07 1998-10-28 Secr Defence Amplification method
DE19840897A1 (de) * 1998-09-08 2000-03-09 Dirk Happich Faktor V-Leiden-Test
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
GB9821989D0 (en) * 1998-10-08 1998-12-02 Hybaid Ltd Detection of nucleic acid polymorphism
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
US6216049B1 (en) 1998-11-20 2001-04-10 Becton, Dickinson And Company Computerized method and apparatus for analyzing nucleic acid assay readings
EP1013775A1 (de) * 1998-12-21 2000-06-28 LUTZ, Hans Quantitative Polymerase-Kettenreaktion mittels fluorogenen Echtzeiterfassungssystem
GB9901072D0 (en) * 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
MXPA01009361A (es) 1999-03-19 2002-06-04 Genencor Int Placa de pruebas de multiples agujeros pasantes para filtracion de alto rendimiento.
US6303305B1 (en) 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
KR20020013519A (ko) * 1999-04-08 2002-02-20 추후제출 보편적 및/또는 축퇴성 염기를 포함하는 안티센스올리고뉴클레오티드
GB9908437D0 (en) * 1999-04-13 1999-06-09 Minton Treharne & Davies Limit Methods of marking materials
CA2644688C (en) * 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
CA2377707A1 (en) * 1999-06-22 2000-12-28 Invitrogen Corporation Improved primers and methods for the detection and discrimination of nucleic acids
EP1206699A4 (de) * 1999-07-27 2005-01-19 Univ Utah Res Found Homogenes fluoreszenzverfahren zum bestimmen struktureller modifikationen von biomolekülen
US6197520B1 (en) * 1999-08-13 2001-03-06 University Of Utah Research Foundation Solution-based color compensation adjusted for temperature and electronic gains
US7192698B1 (en) * 1999-08-17 2007-03-20 Purdue Research Foundation EphA2 as a diagnostic target for metastatic cancer
US6927203B1 (en) * 1999-08-17 2005-08-09 Purdue Research Foundation Treatment of metastatic disease
JP2003514573A (ja) * 1999-11-29 2003-04-22 グラクソ グループ リミテッド ポリ核酸ポリメラーゼについての連続的時間分解共鳴エネルギー転移アッセイ
GB9928232D0 (en) * 1999-12-01 2000-01-26 Skelton Stephen Detection system
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US7700279B2 (en) * 1999-12-24 2010-04-20 Quest Diagnostics Investments Incorporated Assay for bcr/abl gene rearrangement
US7250252B2 (en) * 1999-12-30 2007-07-31 David Aaron Katz Amplification based polymorphism detection
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
GB0005281D0 (en) * 2000-03-07 2000-04-26 Secr Defence Analytical method
US6691041B2 (en) 2000-03-31 2004-02-10 Roche Molecular Systems, Inc. Method for the efficiency-corrected real-time quantification of nucleic acids
DE10045521A1 (de) * 2000-03-31 2001-10-04 Roche Diagnostics Gmbh Nukleinsäureamplifikationen
DE10018465A1 (de) * 2000-04-14 2001-10-18 Aventis Res & Tech Gmbh & Co Testsystem zum Nachweis einer Spleißreaktion, sowie dessen Verwendung
US6783934B1 (en) * 2000-05-01 2004-08-31 Cepheid, Inc. Methods for quantitative analysis of nucleic acid amplification reaction
EP1158449A3 (de) * 2000-05-19 2004-12-15 Becton Dickinson and Company Computerverfahren und Vorrichtung zur Analysierung des Lesens von Nukleinsäuretests
GB0016516D0 (en) * 2000-07-06 2000-08-23 Owen Mumford Ltd Improvements relating to needle protection devices
KR100823857B1 (ko) * 2000-07-07 2008-04-21 아벤티스 파마슈티칼스 인크. 트랜스포존 매개된 다중 서열분석
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
US6849407B2 (en) 2000-08-31 2005-02-01 Mayo Foundation For Medical Education And Research Detection of varicella-zoster virus
US7101976B1 (en) 2000-09-12 2006-09-05 Purdue Research Foundation EphA2 monoclonal antibodies and methods of making and using same
US6558929B2 (en) * 2000-09-15 2003-05-06 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forshung E.V. PCR reaction mixture for fluorescence-based gene expression and gene mutation analyses
US6346386B1 (en) * 2000-09-29 2002-02-12 Arup Institue Method of solution-based scanning for alterations in a DNA segment using a double-stranded DNA binding dye and fluorescence melting profiles
ATE445023T1 (de) * 2000-10-23 2009-10-15 Cancer Rec Tech Ltd Auf nukleinsäurenamplifizierung basierendes verfahren zur bestimmung von methylierungsprofil und reagenzien dafür
GB0025913D0 (en) 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
US6908769B2 (en) * 2000-11-16 2005-06-21 Roche Diagnostics Gmbh Dye pair for fluorescence resonance energy transfer (FRET) measurements
US8900811B2 (en) 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US20030165866A1 (en) * 2001-01-31 2003-09-04 Cockerill Franklin R. Detection of bordetella
AU2002243751A1 (en) 2001-01-31 2002-08-12 Mayo Foundation For Medical Education And Research Detection of herpes simplex virus
US7691571B2 (en) * 2001-01-31 2010-04-06 Mayo Foundation For Medical Education And Research Detection of bordetella
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US7559879B2 (en) * 2001-04-16 2009-07-14 Brunswick Corporation Stride adjustment mechanism
EP1392362B1 (de) 2001-05-07 2008-09-17 Mayo Foundation For Medical Education And Research Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren
US6979530B2 (en) 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
WO2003002959A1 (en) * 2001-06-15 2003-01-09 Mj Research, Inc. Controller for a fluorometer
EP1409738A4 (de) * 2001-06-22 2005-03-30 Marshfield Clinic Verfahren und oligonukleotide zum nachweis von salmonella sp.-,e.coli o157:h7- und listeria-monozytogenen
DE10132211A1 (de) * 2001-06-27 2003-01-16 Epigenomics Ag Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
DE10132785A1 (de) * 2001-07-06 2003-01-16 Clondiag Chip Tech Gmbh Verfahren zum Nachweis von in einer Polymerase-Kettenreaktion amplifizierten Nukleinsäuremolekülen
EP1275735A1 (de) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
US20040209331A1 (en) * 2001-07-16 2004-10-21 Kirk Ririe Thermal cycling system and method of use
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US6979541B1 (en) 2001-07-26 2005-12-27 University Of Utah Research Foundation Methods for identifying chromosomal aneuploidy
US7188030B2 (en) * 2001-08-21 2007-03-06 Applera Corporation Automatic threshold setting for quantitative polymerase chain reaction
DE60216777T2 (de) * 2001-08-23 2007-10-31 Merck & Co., Inc. Multiplex-hpv-pcr-fluoreszenzassays unter verwendung mehrerer fluorophore
EP1288314B1 (de) * 2001-08-31 2006-08-09 The University of Utah Research Foundation Echtzeit-Quantifizierung mit internen Standards
EP1427531B1 (de) * 2001-09-11 2016-10-19 Iquum, Inc. Probenbehälter
US20030082563A1 (en) * 2001-10-15 2003-05-01 Bell Constance A. Detection of bacillus anthracis
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
ATE328119T1 (de) * 2001-11-02 2006-06-15 Roche Diagnostics Gmbh Nachweis von variola virus
WO2003046208A2 (en) * 2001-11-28 2003-06-05 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
US7352694B1 (en) * 2001-12-14 2008-04-01 Applied Micro Circuits Corporation System and method for tolerating data link faults in a packet communications switch fabric
US7424013B1 (en) * 2001-12-20 2008-09-09 Applied Micro Circuits Corporation System and method for granting arbitrated bids in the switching of information
US8418129B1 (en) 2001-12-14 2013-04-09 Qualcomm Incorporated Method for automatically generating code to define a system of hardware elements
US20040018506A1 (en) 2002-01-25 2004-01-29 Koehler Ryan T. Methods for placing, accepting, and filling orders for products and services
US7228237B2 (en) * 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
US6593093B1 (en) 2002-02-20 2003-07-15 Mayo Foundation For Medical Education And Research Detection of group a Streptococcus
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US20050065330A1 (en) * 2002-04-24 2005-03-24 The Cleveland Clinic Foundation Identification of Histoplasma capsulatum using a PCR assay
US7297484B2 (en) * 2002-04-26 2007-11-20 Idaho Technology Characterization of single stranded nucleic acids by melting analysis of secondary structure using double strand-specific nucleic acid dye
AUPS205802A0 (en) * 2002-05-01 2002-06-06 Bio-Molecular Holdings Pty Limited Improved cycling device and method
US7785776B2 (en) 2002-05-13 2010-08-31 Idaho Technology, Inc. Genotyping by amplicon melting curve analysis
US20030215814A1 (en) * 2002-05-17 2003-11-20 Cockerill Franklin R. Detection of Shiga toxin- or Shiga-like toxin-producing organisms
AUPS267802A0 (en) 2002-05-30 2002-06-20 Bio-Molecular Holdings Pty Limited Improved dna amplification apparatus and method
EP1509624A2 (de) * 2002-05-31 2005-03-02 Secretary, Department Of Atomic Energy Methode für den nukleinsäurenachweis basierend auf met/fret wobei sich die fluorophoren gruppen auf komplementären strängen befinden
WO2003106714A1 (en) 2002-06-14 2003-12-24 Gen-Probe Incorporated Compositions and methods for detecting hepatitis b virus
US6896727B2 (en) * 2002-06-28 2005-05-24 Seh America, Inc. Method of determining nitrogen concentration within a wafer
US7083974B2 (en) * 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
US20040219557A1 (en) * 2002-08-01 2004-11-04 Dobrowolski Steven F. Real time PCR assays to detect mutations in the biotinidase gene for newborn screening
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
EP2143803B1 (de) * 2002-09-03 2016-04-13 Quanta Biosciences, Inc. Verbesserte Zusammensetzungen und Verfahren zur cDNA-Synthese
JP2005538734A (ja) * 2002-09-17 2005-12-22 フアルマシア・コーポレーシヨン 自動液体処理中のｒｎａ及び逆転写酵素の保存
WO2004027028A2 (en) * 2002-09-18 2004-04-01 The Trustees Of The University Of Pennsylvania Compositions, methods and kits for detection of an antigen on a cell and in a biological mixture
US7074598B2 (en) * 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
US7365176B2 (en) 2002-09-26 2008-04-29 Mayo Foundation For Medical Education And Research Detection of Epstein-Barr virus
US7074599B2 (en) 2002-09-27 2006-07-11 Mayo Foundation For Medical Education And Research Detection of mecA-containing Staphylococcus spp.
US7919235B2 (en) * 2002-09-30 2011-04-05 F. Hoffman-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
WO2004038038A2 (en) * 2002-10-23 2004-05-06 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
US7718361B2 (en) 2002-12-06 2010-05-18 Roche Molecular Systems, Inc. Quantitative test for bacterial pathogens
DE60333480D1 (de) 2002-12-06 2010-09-02 Roche Diagnostics Gmbh Verfahren zum nachweis von pathogenen organismen
US20040224325A1 (en) 2002-12-20 2004-11-11 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
US7560231B2 (en) * 2002-12-20 2009-07-14 Roche Molecular Systems, Inc. Mannitol and glucitol derivatives
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US7682565B2 (en) * 2002-12-20 2010-03-23 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
US20050048517A1 (en) * 2002-12-20 2005-03-03 Stratagene Compositions and methods for polynucleotide detection
US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
ATE373673T1 (de) * 2003-01-29 2007-10-15 Hoffmann La Roche Verbessertes verfahren zur behandlung durch bisulfit
CA2513690A1 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Solid phase sequencing
EP2574400B1 (de) * 2003-02-05 2016-09-28 Iquum, Inc. Probenverarbeitung
EP1594984A2 (de) * 2003-02-18 2005-11-16 Applera Corporation Verfahren und zusammensetzungen für die gleichzeitige analyse einer vielzahl von polynukleotiden
EP1606419A1 (de) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systeme und methoden zur erhohung der milchproduktion in tieren
US7282331B2 (en) * 2003-03-18 2007-10-16 Advandx, Inc. Method for improved specificity in probe based assays
US20040191776A1 (en) * 2003-03-21 2004-09-30 Pei-Jer Chen Method for genotyping and quantifying Hepatitis B Virus
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
KR20070121853A (ko) 2003-03-31 2007-12-27 에프. 호프만-라 로슈 아게 일본 뇌염 바이러스 혈청군의 구성원을 포함하는, 특정플라비바이러스 검출용 조성물 및 방법
EP2284278A1 (de) 2003-04-04 2011-02-16 Roche Diagnostics GmbH Verbessertes System für Mehrfarben-Echtzeit-PCR
CA2463719A1 (en) * 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20060177824A1 (en) * 2003-04-29 2006-08-10 Procop Gary W Salmonella detection identification
US7148043B2 (en) * 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
DE04752947T1 (de) * 2003-05-23 2006-11-16 Bio-Rad Laboratories, Inc., Hercules Lokalisierte temperaturregelung für raumanordnungen von reaktionsmedien
US20070059701A1 (en) * 2003-06-30 2007-03-15 Astrazeneca Ab Fluorescence assays for nucleic acid polymerase activity
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
EP1502958A1 (de) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US7788039B2 (en) 2003-09-25 2010-08-31 Roche Molecular Systems, Inc. Quantitation of nucleic acids using growth curves
US20070207459A1 (en) * 2003-11-04 2007-09-06 Martin Dugas Method For Distinguishing Immunologically Defined All Subtype
EP1530046A1 (de) * 2003-11-04 2005-05-11 Ludwig-Maximilians-Universität München Methode zur Unterscheidung von AML Subtypen mit verändertem Karyotyp mit mittelmässiger Prognose
US20070212687A1 (en) * 2003-11-04 2007-09-13 Martin Dugas Method For Distinguishing Mll-Ptd-Positive Aml From Other Aml Subtypes
EP1682900A2 (de) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Methode zur unterscheidung von t(11q23)/mll positiven leukemien von t(11q23)/mll negativen leukemien
EP1533618A1 (de) * 2003-11-04 2005-05-25 Ludwig-Maximilians-Universität München Methode zur Unterscheidung prognostisch definierbarer AML
EP1682905A2 (de) * 2003-11-04 2006-07-26 Roche Diagnostics GmbH Verfahren zur unterscheidung cbf-positiver aml-subtypen von cbf-negativen aml-subtypen
US20070128607A1 (en) * 2003-11-04 2007-06-07 Martin Dugas Method for distinguishing aml subtypes with different gene dosages
WO2005043168A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostics Gmbh Method for distinguishing aml-specific flt3 length mutations from tkd mutations
WO2005043161A2 (en) * 2003-11-04 2005-05-12 Roche Diagnostics Gmbh Method for distinguishing leukemia subtypes
US20070105118A1 (en) * 2003-11-04 2007-05-10 Martin Dugas Method for distinguishing aml subtypes with recurring genetic aberrations
EP1680515B1 (de) * 2003-11-06 2009-11-18 Jürg Dual Chemische analyse mit dynamischer viskosimetrie
US7427475B2 (en) * 2003-11-18 2008-09-23 Mayo Foundation For Medical Education And Research Detection of group B streptococcus
EP1689764B1 (de) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Mit mehreren fluorophoren markierte oligonukleotide
US7582452B2 (en) * 2003-11-24 2009-09-01 Life Genetics Lab, Llc Assay for species sources
CA2547743C (en) 2003-12-02 2011-06-28 F. Hoffmann-La Roche Ag Improved method for bisulfite treatment
CA2494571C (en) 2003-12-02 2010-02-09 F.Hoffmann-La Roche Ag Oligonucleotides containing molecular rods
US8697433B2 (en) * 2003-12-10 2014-04-15 Samsung Electronics Co., Ltd. Polymerase chain reaction (PCR) module and multiple PCR system using the same
US20050130213A1 (en) * 2003-12-10 2005-06-16 Tom Morrison Selective ligation and amplification assay
ATE485401T1 (de) * 2004-02-10 2010-11-15 Hoffmann La Roche Neue primer und sonden zum nachweis von parvovirus b19
US20050186606A1 (en) * 2004-02-11 2005-08-25 Schroeder Benjamin G. Methods and compositions for detecting nucleic acids
JP2007525217A (ja) 2004-02-19 2007-09-06 ザ ガバナーズ オブ ザ ユニバーシティー オブ アルバータ レプチンプロモーター多型及びその使用
WO2005085474A2 (en) * 2004-03-02 2005-09-15 Clontech Laboratories Inc. Fluorescent energy transfer labeled nucleic acid substrates and methods of use thereof
US8304192B2 (en) * 2004-03-05 2012-11-06 University Of Toledo Methods and compositions for assessing nucleic acids and alleles
WO2005089945A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
CA2560945C (en) 2004-04-01 2013-06-18 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
WO2005106036A2 (en) 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
CA2562517A1 (en) * 2004-04-16 2005-10-27 Spartan Bioscience Inc. System for rapid nucleic acid amplification and detection
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US20050250112A1 (en) * 2004-05-07 2005-11-10 Padmabandu Gothami A Nucleic acids, compositions, methods, and kits for detecting mycoplasma and acholeplasma species
EP1751314B1 (de) * 2004-05-13 2019-09-18 Nanobiosym, Inc. Nano-pcr: verfahren und vorrichtung zur amplifikation und detektion von nukleinsäuren
DE102004025538A1 (de) * 2004-05-25 2005-12-22 Advalytix Ag Temperierverfahren und -vorrichtung für die Temperaturbehandlung kleiner Flüssigkeitsmengen
EP2471922A1 (de) 2004-05-28 2012-07-04 Asuragen, Inc. Verfahren und Zusammensetzungen mit microRNA
EP1605060A1 (de) * 2004-06-10 2005-12-14 Biodynamics S.R.L. Verfahren und Reaktionsgemisch zur Steigerung der Spezifität und Genauigkeit von Polymerasereaktionen
EP1610170A1 (de) * 2004-06-25 2005-12-28 Sony Deutschland GmbH Eine Methode zum Auftragen einer Teilchenschicht zur Herstellung einer Oberfläche mit Licht-diffusiven Eigenschaften und / oder verringertem Glanz
WO2006012323A1 (en) * 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Dna sequences for the detection of and differentation amongst pathogenic e.coli
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
TWI349704B (en) * 2004-08-10 2011-10-01 Food And Drug Administration Dept Of Health A method for rapidly detecting quinolone-resistant salmonella spp. and the probes and primers utilized therein
WO2006020933A2 (en) * 2004-08-13 2006-02-23 Stratagene California Dual labeled fluorescent probes
EP1632578A1 (de) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
CA2579349A1 (en) 2004-09-07 2006-03-16 Telethon Institute For Child Health Research Agents for treatment or prevention of an allergic disorder
EP1634965B1 (de) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Echtzeit-PCR unter Zusatz von Pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
WO2006034215A2 (en) * 2004-09-20 2006-03-30 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Multiple mode multiplex reaction quenching method
JP2008513028A (ja) 2004-09-21 2008-05-01 アプレラ コーポレイション マイクロＲＮＡ（ｍｉＲＮＡ）の二色リアルタイム／エンドポイント定量化
EP1807537A2 (de) * 2004-10-07 2007-07-18 Chronix Biomedical, INC. Erkennung von nukleinsäuren zur bewertung des risikos der creutzfeldt-jakob-krankheit
US20060078894A1 (en) * 2004-10-12 2006-04-13 Winkler Matthew M Methods and compositions for analyzing nucleic acids
KR101424812B1 (ko) 2004-10-18 2014-08-07 브랜데이스 유니버시티 핵산 증폭을 위한 프라이머, 프로브 및 방법
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
ES2393926T3 (es) 2004-10-21 2013-01-02 Helmholtz Zentrum München Deutsches Forschungszentrum Für Gesundheit Und Umwelt (Gmbh) Gen KASPP (LRRK2), su producción y uso para la detección y tratamiento de trastornos neurodegenerativos
CN103060444B (zh) 2004-10-27 2016-01-13 塞弗德公司 多阶段核酸扩增反应
US20090118132A1 (en) * 2004-11-04 2009-05-07 Roche Molecular Systems, Inc. Classification of Acute Myeloid Leukemia
ES2503743T3 (es) 2004-11-12 2014-10-07 Asuragen, Inc. Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN
EP1841890B1 (de) * 2005-01-03 2013-08-14 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services Primer für nukleinsäurenachweis
WO2006074233A2 (en) 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7315376B2 (en) * 2005-01-07 2008-01-01 Advanced Molecular Systems, Llc Fluorescence detection system
EP1681556B1 (de) 2005-01-18 2007-04-11 Roche Diagnostics GmbH Fluoreszenzabbildung mittels Telezentrizität
ATE357653T1 (de) 2005-01-18 2007-04-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrischer anregungs- und abbildungsoptiken
US8470608B2 (en) * 2008-05-20 2013-06-25 Rapid Pathogen Screening, Inc Combined visual/fluorescence analyte detection test
US8669052B2 (en) * 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
US20060246423A1 (en) * 2005-02-10 2006-11-02 Adelson Martin E Method and kit for the collection and maintenance of the detectability of a plurality of microbiological species in a single gynecological sample
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060178838A1 (en) * 2005-02-10 2006-08-10 Adelson Martin E Method of receiving and handling a plurality of clinical samples for reporting a sum of diagnostic results for each sample
US9464310B2 (en) * 2005-02-10 2016-10-11 Medical Diagnostic Laboratories, Llc Integrated method for collection and maintenance of detectability of a plurality of microbiological agents in a single clinical sample and for handling a plurality of samples for reporting a sum of diagnostic results for each sample
US20060188891A1 (en) * 2005-02-23 2006-08-24 Bickmore William D Jr Methods and apparatus for controlling DNA amplification
JP4398886B2 (ja) * 2005-03-07 2010-01-13 ソニー株式会社 通信端末装置、通信システム、通信方法、およびプログラム
US7759469B2 (en) 2005-03-10 2010-07-20 Roche Diagnostics Operations, Inc. Labeling reagent
EP1700922B1 (de) 2005-03-10 2016-08-24 Roche Diagnostics GmbH 3-Substituierte 5-Nitroindolderivate und diese enthaltende markierte Oligonukleotidsonden
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
EP2348320B1 (de) 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
US7680604B2 (en) 2005-03-11 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by rotational transform after zero slope alignment
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
WO2006116276A2 (en) * 2005-04-28 2006-11-02 Merck & Co., Inc. Real-time hpv pcr assays
JP2008539730A (ja) * 2005-05-02 2008-11-20 ストラタジーン カリフォルニア オリゴヌクレオチドのプローブ／プライマー組成物、及びポリヌクレオチドの検出方法
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
US8357801B2 (en) 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
AU2006341607B2 (en) * 2005-05-31 2011-03-17 Applied Biosystems, Llc. Multiplexed amplification of short nucleic acids
US20060281099A1 (en) * 2005-06-14 2006-12-14 Bio-Rad Laboratories, Inc. Q-melt for polymorphism detection
SG162795A1 (en) 2005-06-15 2010-07-29 Callida Genomics Inc Single molecule arrays for genetic and chemical analysis
US20060286590A1 (en) * 2005-06-20 2006-12-21 Applera Corporation. Cis-regulatory modules
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
GB0514889D0 (en) 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
PL1937834T3 (pl) * 2005-09-01 2014-11-28 Mtpcr Pty Ltd Sposoby wzmacniania, kwantyfikacji i identyfikacji kwasów nukleinowych
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US7991558B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Systems and methods for determining real-time PCR cycle thresholds using cluster analysis
US7991561B2 (en) 2005-09-29 2011-08-02 Roche Molecular Systems, Inc. Ct determination by cluster analysis with variable cluster endpoint
WO2007041201A2 (en) 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
EP1951900B1 (de) 2005-10-07 2016-06-15 Callida Genomics, Inc. Selbstzusammengesetzte einzelmolekül-arrays und verwendungen davon
US20090155894A1 (en) * 2005-10-17 2009-06-18 Soper Steven A Electrokinetic Thermal Cycler and Reactor
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
WO2007120208A2 (en) * 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
EP2468901B1 (de) 2005-11-29 2017-04-05 Cambridge Enterprise Limited Marker für Brustkrebs
EP1798291B1 (de) * 2005-12-13 2011-05-18 Institut Curie Verfahren für die Detektion von Mutationen in Nukleinsaüren, und die Anwendung in der Diagnose von genetischen Krankheiten und Krebs
WO2007068437A1 (en) * 2005-12-14 2007-06-21 Roche Diagnostics Gmbh New method for bisulfite treatment
EP1798542A1 (de) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytisches Verfahren und Gerät
EP1798650A1 (de) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytisches Verfahren und Instrument
US7844403B2 (en) 2005-12-20 2010-11-30 Roche Molecular Systems, Inc. Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
US7668663B2 (en) 2005-12-20 2010-02-23 Roche Molecular Systems, Inc. Levenberg-Marquardt outlier spike removal method
US7680868B2 (en) 2005-12-20 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization
US7991562B2 (en) 2005-12-20 2011-08-02 Roche Molecular Systems, Inc. PCR elbow determination using curvature analysis of a double sigmoid
US7981606B2 (en) * 2005-12-21 2011-07-19 Roche Molecular Systems, Inc. Control for nucleic acid testing
CA2637600A1 (en) 2006-01-17 2007-07-26 Health Research, Inc. Heteroduplex tracking assay
US20090305238A1 (en) * 2006-01-23 2009-12-10 Applera Corporation Microarray Microcard
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
GB0603190D0 (en) * 2006-02-16 2006-03-29 Enigma Diagnostics Ltd Detection system
CA2638854C (en) * 2006-02-27 2012-05-15 F. Hoffmann-La Roche Ag Pcr hot start by magnesium sequestration
US20110143344A1 (en) * 2006-03-01 2011-06-16 The Washington University Genetic polymorphisms and substance dependence
WO2007100919A2 (en) * 2006-03-01 2007-09-07 Perlegen Sciences, Inc. Markers for addiction
RU2394915C2 (ru) * 2006-03-24 2010-07-20 Александр Борисович Четверин Бесконтактные способы обнаружения молекулярных колоний, наборы реагентов и устройство для их осуществления
US8778637B2 (en) 2006-03-28 2014-07-15 Canon U.S. Life Sciences, Inc. Method and apparatus for applying continuous flow and uniform temperature to generate thermal melting curves in a microfluidic device
EP3167900B1 (de) 2006-03-29 2018-11-21 Merial Limited Impfstoff gegen streptokokken
EP2002013A4 (de) 2006-04-04 2010-05-05 Labonnet Ltd Beurteilung der reaktionskinetik-kompatibilität zwischen polymerasekettenreaktionen
US20070238108A1 (en) * 2006-04-07 2007-10-11 Agilent Technologies, Inc. Validation of comparative genomic hybridization
US20070238095A1 (en) * 2006-04-11 2007-10-11 Mayo Foundation For Medical Education And Research , A Minnesota Corporation Detection of Influenza A Virus
US20070238093A1 (en) * 2006-04-11 2007-10-11 Espy Mark J Detection of influenza A virus
US9862984B2 (en) 2006-04-21 2018-01-09 Nanobiosym, Inc. Single-molecule platform for drug discovery: methods and apparatuses for drug discovery, including discovery of anticancer and antiviral agents
WO2007127992A2 (en) 2006-04-28 2007-11-08 Igor Kutyavin Use of base-modified deoxynucleoside triphosphates
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
EP2535427A3 (de) * 2006-05-17 2013-04-24 California Institute of Technology Thermisches Wechselbeanspruchungssystem
US8232091B2 (en) * 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
US8637436B2 (en) 2006-08-24 2014-01-28 California Institute Of Technology Integrated semiconductor bioarray
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
WO2007143669A2 (en) * 2006-06-05 2007-12-13 California Institute Of Technology Real time micro arrays
US8119352B2 (en) 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US7629124B2 (en) * 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US8048626B2 (en) * 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
WO2008021010A2 (en) * 2006-08-07 2008-02-21 Stratagene California Methods for real-time quantitative pcr
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
EP1900824A1 (de) * 2006-09-14 2008-03-19 Deutsches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts Genexpressionsprofile zur Prognose, Diagnose und Therapie von Prostatakrebs und deren Verwendung
EP2145001A2 (de) * 2006-09-19 2010-01-20 Asuragen, Inc. Von mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulierte gene und stoffwechselwege als ziele für einen therapeutischen eingriff
WO2008063371A2 (en) * 2006-11-02 2008-05-29 Immunivest Corporation Imaging of activated vascular endothelium using immunomagnetic mri contrast agents
US9114398B2 (en) 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CN101622349A (zh) * 2006-12-08 2010-01-06 奥斯瑞根公司 作为治疗性干预靶标的miR-21调节的基因和途径
WO2008073919A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
CA2671294A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
CA2671299A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro rnas
WO2008074023A2 (en) 2006-12-13 2008-06-19 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US20120144508A1 (en) 2006-12-21 2012-06-07 Ben Hayes Artificial selection method and reagents
CA2671270A1 (en) * 2006-12-29 2008-07-17 Asuragen, Inc. Mir-16 regulated genes and pathways as targets for therapeutic intervention
US8535888B2 (en) 2006-12-29 2013-09-17 Mayo Foundation For Medical Education And Research Compositions and methods for detecting methicillin-resistant S. aureus
EP2126114B1 (de) * 2007-02-01 2016-09-28 Abacus Diagnostica OY Verfahren zum nachweis des vorliegens von zielpolynukleotid in proben
CN107090480A (zh) 2007-02-02 2017-08-25 杰纳罗生物系统有限公司 核酸分子的生成
US20100190167A1 (en) * 2007-02-14 2010-07-29 Genisphere Inc. Methods, Reagents and Kits for Detection of Nucleic Acid Molecules
JP4942508B2 (ja) * 2007-02-20 2012-05-30 アークレイ株式会社 ａｂｌ遺伝子変異の検出用プローブおよびその用途
US9475051B2 (en) * 2007-02-27 2016-10-25 Sony Corporation Nucleic acid amplifier
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
CN101903535A (zh) 2007-03-28 2010-12-01 信号诊断公司 高解析度分析核酸以检测序列变异的系统和方法
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
EP2503008B1 (de) 2007-04-19 2015-04-01 Molecular Detection Inc. Verfahren, Zusammensetzungen und Kits zur Erkennung und Analyse von Antibiotika-resistenten Bakterien
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
CA2689974A1 (en) * 2007-06-08 2008-12-18 Asuragen, Inc. Mir-34 regulated genes and pathways as targets for therapeutic intervention
DE102007031137A1 (de) * 2007-06-13 2008-12-18 Attomol Gmbh Molekulare Diagnostika Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets
EP2167685B1 (de) * 2007-06-13 2014-12-17 Attomol GmbH Molekulare Diagnostika Verfahren und sonden/primersystem zum "real time" nachweis eines nukleinsäuretargets
JP5282088B2 (ja) 2007-06-21 2013-09-04 ジェン−プロウブ インコーポレイテッド 処理実行に使用する機器および容器
EP2395113A1 (de) 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Verfahren und Zusammensetzungen zur Isolierung von Nukleinsäuresequenzvarianten
US20090023138A1 (en) * 2007-07-17 2009-01-22 Zila Biotechnology, Inc. Oral cancer markers and their detection
US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US8386184B2 (en) 2007-08-28 2013-02-26 Becton, Dickinson And Company Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
US8380457B2 (en) 2007-08-29 2013-02-19 Canon U.S. Life Sciences, Inc. Microfluidic devices with integrated resistive heater electrodes including systems and methods for controlling and measuring the temperatures of such heater electrodes
DE102007041864B4 (de) 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
EP2198050A1 (de) * 2007-09-14 2010-06-23 Asuragen, INC. Mikrornas mit unterschiedlicher expression bei zervikalkarzinom und verwendungen davon
US20090119020A1 (en) 2007-09-25 2009-05-07 Roche Molecular Systems, Inc. Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid
EP2209909B1 (de) 2007-09-28 2011-04-13 3M Innovative Properties Company Doppel-oligonukleotid-nukleinsäurenachweisverfahren
WO2009052386A1 (en) * 2007-10-18 2009-04-23 Asuragen, Inc. Micrornas differentially expressed in lung diseases and uses thereof
FR2922897B1 (fr) * 2007-10-25 2009-11-27 Bio Rad Pasteur Mesure d'une population d'acides nucleiques,en particulier par pcr en temps reel.
US8180572B2 (en) * 2007-10-25 2012-05-15 Canon U.S. Life Sciences, Inc. High-resolution melting analysis
SG185935A1 (en) * 2007-10-30 2012-12-28 Complete Genomics Inc Apparatus for high throughput sequencing of nucleic acids
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
US20090137058A1 (en) * 2007-11-28 2009-05-28 General Electric Company Chemical and biological detection method and device based on measurements of fluorescence and reflectivity
US8071562B2 (en) * 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
EP2071041A1 (de) * 2007-12-13 2009-06-17 Hitachi High-Technologies Corporation Sonde zum Nachweisen von Nukleinsäure
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
WO2009081965A1 (ja) * 2007-12-26 2009-07-02 Arkray, Inc. 融解曲線解析方法および融解曲線解析装置
US8306754B2 (en) * 2007-12-26 2012-11-06 Arkray, Inc. Nucleic acid amplification determining method and nucleic acid amplification determining device
DE102007063102B4 (de) 2007-12-28 2022-02-10 Robert Bosch Gmbh Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters
WO2009092564A2 (en) * 2008-01-23 2009-07-30 Roche Diagnostics Gmbh Integrated instrument performing synthesis and amplification
JP5665548B2 (ja) 2008-02-08 2015-02-04 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ クロストリジウム・ディフィシレの検出
EP2260110B1 (de) * 2008-02-08 2014-11-12 Asuragen, INC. In lymphknoten von krebspatienten differentiell exprimierte mirnas
WO2009103003A2 (en) * 2008-02-15 2009-08-20 Bio-Rad Laboratories, Inc. Scanning fluorescent reader with diffuser system
WO2009111475A2 (en) 2008-03-03 2009-09-11 Heatflow Technologies, Inc. Heat flow polymerase chain reaction systems and methods
EP2291541A4 (de) * 2008-03-05 2011-06-22 Clean Earth Tech Llc Mit multiplex-pcr gekoppelter durchflusszytometrischer test mit pna-markierten beads zum gleichzeitigen nachweis mehrerer biologischer stoffe
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
WO2009154835A2 (en) * 2008-03-26 2009-12-23 Asuragen, Inc. Compositions and methods related to mir-16 and therapy of prostate cancer
US20090258928A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc. Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
EP2108699B1 (de) 2008-04-08 2014-06-25 F.Hoffmann-La Roche Ag Vorrichtung zur analytischen Verarbeitung und Detektion
ATE537272T1 (de) * 2008-04-08 2011-12-15 Hoffmann La Roche Detektion von pcr-produkten in der gelelektrophorese
WO2009137807A2 (en) 2008-05-08 2009-11-12 Asuragen, Inc. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
US8609433B2 (en) * 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
EP2130929B1 (de) 2008-06-06 2013-10-09 F. Hoffmann-La Roche AG Intern gesteuerte Multiplex-Erkennung und Quantifizierung von mikrobiellen Nukleinsäuren
US20110086359A1 (en) * 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
EP2138588A1 (de) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Messung der Schmelzkurve während der Amplifizierung
US8097412B2 (en) 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
DE102008040513B4 (de) * 2008-07-17 2010-08-26 BAM Bundesanstalt für Materialforschung und -prüfung Verwendung einer langwellig emittierenden Cyaninverbindung als NIR-Fluoreszenzstandard und Kit zur Kalibrierung von Photolumineszenzmesssystemen
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
GB0909333D0 (en) 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
CA2638458A1 (en) * 2008-07-31 2010-01-31 Spartan Bioscience Inc. Thermal recycling by positioning relative to fixed-temperature source
EP2310527B1 (de) 2008-08-01 2015-04-29 Roche Diagnostics GmbH Verbesserte lysis und reverse transkription zur mrna-quantifizierung
US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
JP5166608B2 (ja) * 2008-09-09 2013-03-21 バイオ−ラッド ラボラトリーズ，インコーポレイティド 多段階回帰ベースｐｃｒ解析システム
US8219324B2 (en) 2008-09-12 2012-07-10 Roche Molecular Systems, Inc. Real-time PCR elbow calling by equation-less algorithm
US9540686B2 (en) * 2008-09-18 2017-01-10 Canon U.S. Life Sciences, Inc. Systems and methods for the amplification of DNA
US8170804B2 (en) * 2008-10-17 2012-05-01 Roche Molecular Systems, Inc. Determination of melting temperatures of DNA
CA2682439A1 (en) 2008-10-17 2010-04-17 F. Hoffmann-La Roche Ag Cell monitoring and molecular analysis
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
CN102272327B (zh) 2008-11-07 2015-11-25 赛昆塔公司 通过序列分析监测状况的方法
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US20100179213A1 (en) * 2008-11-11 2010-07-15 Mirna Therapeutics, Inc. Methods and Compositions Involving miRNAs In Cancer Stem Cells
US10669574B2 (en) 2008-11-18 2020-06-02 XCR Diagnostics, Inc. DNA amplification technology
WO2010067055A1 (en) 2008-12-09 2010-06-17 Oxitec Ltd. Enhanced taqman probe based amplification
WO2010077324A2 (en) 2008-12-17 2010-07-08 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
CA2688174C (en) 2008-12-19 2018-08-07 F. Hoffmann-La Roche Ag Dry composition of reaction compounds with stabilized polymerase
US20100159452A1 (en) * 2008-12-22 2010-06-24 Roche Molecular Systems, Inc. Method For Detecting a Target Nucleic Acid in a Sample
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
CA2749093C (en) * 2009-01-08 2019-03-26 Bio-Rad Laboratories, Inc. Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions
EA020593B1 (ru) 2009-01-13 2014-12-30 Флуидигм Корпорейшн Анализ нуклеиновых кислот, полученных из единичных клеток
PT2387627E (pt) 2009-01-15 2016-06-03 Adaptive Biotechnologies Corp Determinação do perfil de imunidade adaptativa e métodos de geração de anticorpos monoclonais
WO2010086410A1 (en) 2009-01-30 2010-08-05 Kantonsspital Aarau Ag Gene dosage analysis
EP2391452B1 (de) 2009-01-30 2015-06-17 Gen-Probe Incorporated Systeme und verfahren zur erfassung eines signals und beaufschlagung des signalübertragungselements mit wärmeenergie
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (ja) * 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
US8606527B2 (en) * 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US9542526B2 (en) * 2009-03-10 2017-01-10 Canon U.S. Life Sciences, Inc. Method and system for temperature correction in thermal melt analysis
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
EP2411543B1 (de) 2009-03-27 2017-04-19 Life Technologies Corporation Verfahren, zusammensetzungen und kits für den nachweis von allelen varianten
GB0905325D0 (en) 2009-03-30 2009-05-13 Selex Sensors & Airborne Sys Detection system
US20100279299A1 (en) * 2009-04-03 2010-11-04 Helixis, Inc. Devices and Methods for Heating Biological Samples
US20120028819A1 (en) 2009-04-07 2012-02-02 Koninklijke Philips Electronics N.V. Method for the detection and characterization of a toxinogenic clostridium difficile strain
CA2758824A1 (en) 2009-04-17 2010-10-21 Basf Plant Science Company Gmbh Plant promoter operable in endosperm and uses thereof
WO2010133972A1 (en) 2009-05-22 2010-11-25 Population Genetics Technologies Ltd Sorting asymmetrically tagged nucleic acids by selective primer extension
CN102449167B (zh) 2009-05-26 2014-12-17 厦门大学 一种检测核酸序列变异的方法
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
EP2435461B1 (de) 2009-05-29 2017-08-09 Life Technologies Corporation Nukleinsäuregerüst-polymerpartikel und verfahren zu ihrer herstellung und verwendung
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
SG176691A1 (en) 2009-06-25 2012-01-30 Hutchinson Fred Cancer Res Method of measuring adaptive immunity
JP5575239B2 (ja) * 2009-07-21 2014-08-20 ジェン−プローブ・インコーポレーテッド 拡張されたダイナミックレンジにわたる核酸配列の定量的検出のための方法および組成物
WO2011022589A2 (en) * 2009-08-20 2011-02-24 Mayo Foundation For Medical Education And Research Detection of enterovirus
US8298767B2 (en) 2009-08-20 2012-10-30 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
FI20090306A0 (fi) 2009-08-25 2009-08-25 Qret Technologies Oy Erotusvapaa määritysmenetelmä
US8219366B2 (en) 2009-08-26 2012-07-10 Roche Molecular Sytems, Inc. Determination of elbow values for PCR for parabolic shaped curves
US8987685B2 (en) * 2009-09-09 2015-03-24 Pcr Max Limited Optical system for multiple reactions
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
WO2011053845A2 (en) 2009-10-30 2011-05-05 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US20110111403A1 (en) * 2009-10-30 2011-05-12 Life Technologies Corporation Multi-primer assay for mycoplasma detection
WO2011055232A2 (en) 2009-11-04 2011-05-12 Population Genetics Technologies Ltd. Base-by-base mutation screening
US9133343B2 (en) 2009-11-30 2015-09-15 Enzo Biochem, Inc. Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
EP2524053A1 (de) * 2010-01-15 2012-11-21 Steffen Mergemeier Verfahren zur erkennung mehr als eines ziels in einem pcr-basierten ansatz mit einer unspezifischen und nicht mit der emission fluorphor-etikettierter sonden interferierenden matrize
US20110177512A1 (en) * 2010-01-19 2011-07-21 Predictive Biosciences, Inc. Method for assuring amplification of an abnormal nucleic acid in a sample
US20130053253A1 (en) 2010-02-22 2013-02-28 Population Genetics Technologies Ltd Region of Interest Extraction and Normalization Methods
WO2011107887A2 (en) 2010-03-02 2011-09-09 Population Genetic Technologies Ltd. Methods for replicating polynucleotides with secondary structure
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
JP5843112B2 (ja) * 2010-03-29 2016-01-13 凸版印刷株式会社 標的塩基配列の識別方法
DE102010003781B4 (de) 2010-04-08 2012-08-16 Aj Innuscreen Gmbh Verfahren zum Nachweis spezifischer Nukleinsäuresequenzen
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
WO2011138402A1 (en) 2010-05-05 2011-11-10 Check-Points Holding B.V. Assays, compositions and methods for detecting drug resistant micro-organisms
DK3144673T3 (da) 2010-05-06 2019-09-09 Adaptive Biotechnologies Corp Overvågning af en lymfoid neoplasma anvendende klontypeprofiler
GB201007868D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Sequence detection assay
GB201007867D0 (en) 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Signalling system
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
AU2011226792A1 (en) 2010-06-11 2012-01-12 Life Technologies Corporation Alternative nucleotide flows in sequencing-by-synthesis methods
US9096952B2 (en) 2010-06-24 2015-08-04 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
DE112011102524T5 (de) 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Präparation generischer Proben
CA2802550C (en) 2010-07-29 2019-01-15 F. Hoffmann-La Roche Ag Generic pcr
ES2605303T3 (es) 2010-07-29 2017-03-13 F. Hoffmann-La Roche Ag Detección cualitativa y cuantitativa de ácidos nucleicos microbianos
WO2012013734A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Control nucleic acids for multiple parameters
CN103210079B (zh) 2010-08-02 2015-07-22 B·L·韦特 用于聚合酶链式反应的可增压筒
JP5959516B2 (ja) 2010-08-18 2016-08-02 ライフ テクノロジーズ コーポレーション 電気化学的検出装置のためのマイクロウェルの化学コーティング法
WO2012034130A2 (en) 2010-09-10 2012-03-15 Qiagen Methods and compositions for nucleic acid detection
ES2707341T3 (es) 2010-09-13 2019-04-03 Clinical Genomics Pty Ltd Diagnóstico de cáncer mediante marcador de metilación
CA2811185C (en) 2010-09-21 2020-09-22 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
WO2012038049A2 (en) 2010-09-22 2012-03-29 Roche Diagnostics Gmbh Amplification of distant nucleic acid targets using engineered primers
JP6174999B2 (ja) 2010-10-04 2017-08-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｐｃｒ反応緩衝液中での細胞溶解のための方法
CA2810316A1 (en) 2010-10-04 2012-04-12 F. Hoffmann-La Roche Ag Method for cell lysis and pcr within the same reaction vessel
JP5798631B2 (ja) 2010-10-04 2015-10-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｒｔ−ｐｃｒ反応緩衝液中での細胞溶解のための方法
CA2814049C (en) 2010-10-08 2021-07-13 President And Fellows Of Harvard College High-throughput single cell barcoding
CA2814047C (en) 2010-10-08 2017-11-14 President And Fellows Of Harvard College High-throughput immune sequencing
GB201017011D0 (en) 2010-10-08 2010-11-24 Skova Services Ltd Assay
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
KR101420094B1 (ko) 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
SG10201407036YA (en) 2010-11-05 2014-12-30 Transbio Ltd Markers of Endothelial Progenitor Cells and Uses Thereof
RU2451086C1 (ru) * 2010-12-03 2012-05-20 Государственное образовательное учреждение высшего профессионального образования Смоленская государственная медицинская академия федерального агентства по здравоохранению и социальному развитию Способ детекции специфических нуклеотидных последовательностей и нуклеотидных замен с помощью пцр в режиме реального времени с эффектом гашения флуоресценции зонда праймером
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
EP2465945A1 (de) 2010-12-17 2012-06-20 F. Hoffmann-La Roche AG Allgemeine Matrix zur Steuerung von Nukleinsäuren
WO2012087135A1 (en) 2010-12-22 2012-06-28 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Genetic markers specific for clostridium difficile ribotypes 027 (nap01/b1; rt 027) and 078 (nap7/8; rt 078) and their use
EP2659408B1 (de) 2010-12-29 2019-03-27 Life Technologies Corporation Zeitverzerrtes hintergrundsignal für sequenzierung-mit-synthese-operationen
US20130060482A1 (en) 2010-12-30 2013-03-07 Life Technologies Corporation Methods, systems, and computer readable media for making base calls in nucleic acid sequencing
WO2012092455A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
WO2012092515A2 (en) 2010-12-30 2012-07-05 Life Technologies Corporation Methods, systems, and computer readable media for nucleic acid sequencing
EP2663652B1 (de) 2011-01-11 2018-11-14 Roche Diagniostics GmbH Schmelzanalyse mit hoher auflösung als voruntersuchungsinstrument
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US9096892B1 (en) 2011-02-04 2015-08-04 K2 Biomicrosystems, LLC Nucleic acid amplification and monitoring apparatus
WO2012108864A1 (en) 2011-02-08 2012-08-16 Illumina, Inc. Selective enrichment of nucleic acids
FR2971257B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971258B1 (fr) 2011-02-09 2020-12-04 Bio Rad Pasteur Combinaison de biomarqueurs pour le pronostic d'une reponse ou non-reponse a un traitement anti-vhc
FR2971256A1 (fr) 2011-02-09 2012-08-10 Bio Rad Pasteur Combinaison de biomarqueurs pour la detection et l'evaluation d'une fibrose hepatique
CA3043100C (en) 2011-03-08 2022-03-01 Universite Laval Fluidic centripetal device
EP3878975A1 (de) 2011-04-08 2021-09-15 Life Technologies Corporation Phasenschützende reagenzflussordnungen für die verwendung zur sequenzierung durch synthese
EP2697657B8 (de) 2011-04-15 2017-08-16 Becton, Dickinson and Company Mikrofluidischer abtastender echtzeit-thermocycler und verfahren zur optischen detektion mittels synchronisierter thermozyklierung und abtastung
WO2012142656A1 (en) 2011-04-18 2012-10-26 Garvan Institute Of Medical Research Method of diagnosing cancer
WO2012151560A2 (en) * 2011-05-04 2012-11-08 Biocept, Inc. Methods for detecting nucleic acid sequence variants
CN108342453A (zh) 2011-05-09 2018-07-31 富鲁达公司 基于探针的核酸检测
WO2012153153A1 (en) 2011-05-11 2012-11-15 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
EP2710146A2 (de) 2011-05-18 2014-03-26 Life Technologies Corporation Chromosomenkonformationsanalyse
DK2714939T3 (en) 2011-05-24 2015-06-29 Elitech Holding Bv DETECTION OF METHICILLIN-RESISTENT STAPHYLOCOCCUS AUREUS
US9034581B2 (en) 2011-05-26 2015-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Staphylococcus aureus
US9737891B2 (en) * 2011-06-01 2017-08-22 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
EP2721173A1 (de) 2011-06-14 2014-04-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Verfahren zur bestimmung des expressionsniveaus eines bestimmten gens mit korrektur von rt-qpcr-daten für signale aus der genomischen dna
EP2745108B1 (de) 2011-08-18 2019-06-26 Life Technologies Corporation Verfahren, systeme und computerlesbare medien zur herstellung von basenzuordnungen in einer nukleinsäuresequenzierung
AU2012296385A1 (en) 2011-08-18 2014-02-20 Nestec S.A. Compositions and methods for detecting allelic variants
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
ES2684525T3 (es) 2011-09-15 2018-10-03 David A. Shafer Composiciones de sonda:antisonda para detección de ADN o ARN de alta especificidad
EP2570487A1 (de) 2011-09-16 2013-03-20 Lexogen GmbH Verfahren zur Nukleinsäuretranskription
ES2686043T3 (es) 2011-09-16 2018-10-16 Lexogen Gmbh Método para preparar una biblioteca de moléculas de ácido nucleico
ES2656961T3 (es) 2011-09-23 2018-03-01 F. Hoffmann-La Roche Ag Uso de G-Clamp para mejorar la PCR específica de alelo
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
EP2768607B1 (de) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell-platte für die hocheffiziente kleinvolumige nukleinsäuresynthese
EP2761032B1 (de) 2011-09-29 2017-07-19 Luminex Corporation Hydrolysesonden
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
CN103998921B (zh) * 2011-10-14 2016-04-20 贝克顿·迪金森公司 方波热循环
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
US9279159B2 (en) 2011-10-21 2016-03-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
US9447458B2 (en) 2011-11-16 2016-09-20 Canon U.S. Life Sciences, Inc. Detection of neighboring variants
US9260714B2 (en) 2011-12-02 2016-02-16 Roche Molecular Systems, Inc. Suppression of non-specific amplification with high-homology oligonucleotides
ES2683037T3 (es) 2011-12-09 2018-09-24 Adaptive Biotechnologies Corporation Diagnóstico de tumores malignos linfoides y detección de enfermedad residual mínima
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
WO2013093432A1 (en) 2011-12-19 2013-06-27 David Kelly Method for enriching and detection of variant target nucleic acids
US8981318B1 (en) 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
US9194840B2 (en) 2012-01-19 2015-11-24 Life Technologies Corporation Sensor arrays and methods for making same
WO2013112458A1 (en) 2012-01-24 2013-08-01 Beth Israel Deaconess Medical Center, Inc. Novel chrebp isoforms and methods using the same
US9864846B2 (en) 2012-01-31 2018-01-09 Life Technologies Corporation Methods and computer program products for compression of sequencing data
US9515676B2 (en) 2012-01-31 2016-12-06 Life Technologies Corporation Methods and computer program products for compression of sequencing data
WO2013113748A1 (en) * 2012-02-02 2013-08-08 Primer Design Ltd Method for detecting and genotyping target nucleic acid
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
EP3287531B1 (de) 2012-02-28 2019-06-19 Agilent Technologies, Inc. Verfahren zur befestigung einer zählersequenz an einer nukleinsäureprobe
ES2662128T3 (es) 2012-03-05 2018-04-05 Adaptive Biotechnologies Corporation Determinación de cadenas de receptor inmunitario emparejadas a partir de la frecuencia de subunidades coincidentes
WO2013133561A1 (en) 2012-03-05 2013-09-12 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
US9932633B2 (en) 2012-03-21 2018-04-03 Roche Molecular Systems, Inc. Methods for assessing RNA quality
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
JP5756247B1 (ja) 2012-05-08 2015-07-29 アダプティブ バイオテクノロジーズ コーポレイション マルチプレックスｐｃｒ反応における増幅バイアスを測定し校正する組成物及び方法
EP3594366B1 (de) 2012-05-11 2021-06-23 Clinical Genomics Pty. Ltd. Diagnostisches genmarkerpaneel
US9646132B2 (en) 2012-05-11 2017-05-09 Life Technologies Corporation Models for analyzing data from sequencing-by-synthesis operations
AU2013262435B2 (en) 2012-05-18 2019-03-07 Clinical Genomics Pty. Ltd. A method of screening for colorectal cancer
EP3553181A1 (de) * 2012-05-25 2019-10-16 Accugenomics, Inc. Nukleinsäureamplifikation und anwendung davon
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
CN110343673B (zh) 2012-06-14 2024-04-05 生命技术公司 用于聚合酶链式反应(pcr)的新型组合物、方法和试剂盒
US9384320B2 (en) 2012-07-19 2016-07-05 President And Fellows Of Harvard College Methods of storing information using nucleic acids
WO2014018885A2 (en) 2012-07-27 2014-01-30 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
US9791372B2 (en) 2012-08-03 2017-10-17 California Institute Of Technology Multiplexing and quantification in PCR with reduced hardware and requirements
US9957557B2 (en) 2012-08-13 2018-05-01 Life Genetics Lab, Llc Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples
US10993418B2 (en) 2012-08-13 2021-05-04 Life Genetics Lab, Llc Method for measuring tumor burden in patient derived xenograft (PDX) mice
US9982313B2 (en) 2012-08-17 2018-05-29 Roche Molecular Systems, Inc. Compositions and methods for detection of herpes simplex virus 1 and 2
US10053726B2 (en) * 2012-09-10 2018-08-21 Biofire Diagnostics, Llc Multiple amplification cycle detection
US9914968B2 (en) 2012-09-26 2018-03-13 Cepheid Honeycomb tube
EP2904111B1 (de) 2012-10-01 2017-12-06 Adaptive Biotechnologies Corporation Immunkompetenzbeurteilung durch adaptive immunrezeptordiversität und klonalitätscharakterisierung
US10176293B2 (en) 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
WO2014058919A1 (en) * 2012-10-09 2014-04-17 University Of Utah Research Foundation Monitoring temperature with fluorescence
US10329608B2 (en) 2012-10-10 2019-06-25 Life Technologies Corporation Methods, systems, and computer readable media for repeat sequencing
EP2722399A1 (de) 2012-10-18 2014-04-23 Roche Diagniostics GmbH Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
CN103114131B (zh) 2012-11-30 2018-10-02 珠海市坤元农业科技有限公司 一种引物中部序列干扰pcr技术
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
CN105121661B (zh) 2013-02-01 2018-06-08 加利福尼亚大学董事会 用于基因组组装及单体型定相的方法
EP2770065B1 (de) 2013-02-25 2017-12-13 Seegene, Inc. Detektion einer Nukleotidvariation auf Nukleinsäurezielsequenz
EP2971115B1 (de) 2013-03-13 2022-07-27 Seegene, Inc. Quantifizierung von zielnukleinsäuren unter verwendung von schmelzspitzenwertanalyse
US20140296080A1 (en) 2013-03-14 2014-10-02 Life Technologies Corporation Methods, Systems, and Computer Readable Media for Evaluating Variant Likelihood
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
CN105378487B (zh) 2013-03-15 2018-08-21 雅培制药有限公司 具有竖直布置的圆盘传送带的自动化诊断分析仪及相关方法
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
ES2970108T3 (es) 2013-03-15 2024-05-27 Abbott Lab Analizadores de diagnóstico con carruseles de pretratamiento y métodos relacionados
US20140273181A1 (en) 2013-03-15 2014-09-18 Biofire Diagnostics, Inc. Compact optical system for substantially simultaneous monitoring of samples in a sample array
EP3964839B1 (de) 2013-03-15 2024-04-10 Abbott Laboratories Automatisierte diagnostische analysatoren mit rückwärtig zugänglichen führungsspursystemen und zugehörige verfahren
EP3486328B1 (de) 2013-03-15 2020-11-11 Avellino Lab USA, Inc. Verfahren zur verbesserten isolierung von genomischen dna-matrizen zum nachweis von allelen
EP3312295A1 (de) 2013-03-19 2018-04-25 Directed Genomics, LLC Anreicherung von zielsequenzen
WO2014174143A1 (en) 2013-04-23 2014-10-30 Thermo Fisher Scientific Oy Melt curve analysis
JP6583794B2 (ja) 2013-05-20 2019-10-02 バイオベンチャーズ・リミテッド・ライアビリティ・カンパニーＢｉｏＶｅｎｔｕｒｅｓ， ＬＬＣ 多発性骨髄腫のためのｇｅｐ５モデル
EP3957743A1 (de) 2013-06-19 2022-02-23 Luminex Corporation Multiplexierter echtzeithydrolysesondentest
CN111349692B (zh) 2013-06-25 2023-10-13 C.威特沃 进行聚合酶链式反应的方法及其相关应用
CA2916990C (en) 2013-06-28 2023-05-23 Streck, Inc. Devices for real-time polymerase chain reaction
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
WO2015008985A1 (en) 2013-07-15 2015-01-22 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent immobilized oligonucleotide hybridization
US9926597B2 (en) 2013-07-26 2018-03-27 Life Technologies Corporation Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same
US9657332B2 (en) 2013-08-09 2017-05-23 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2015022669A2 (en) 2013-08-15 2015-02-19 Centre Hospitalier Universitarie Vaudois Methods for typing hla alleles
CN105683980B (zh) 2013-10-04 2018-08-24 生命科技股份有限公司 在使用终止化学物质的测序中建立分阶段效应模型的方法和系统
KR102323375B1 (ko) 2013-10-09 2021-11-08 플루어레센트릭 인코포레이티드 다중 프로브
US10072288B2 (en) 2013-11-11 2018-09-11 Roche Molecular Systems, Inc. Detecting single nucleotide polymorphism using overlapped primer and melting probe
KR102293785B1 (ko) 2013-11-15 2021-08-26 아벨리노 랩 유에스에이, 인크. 안과 질환과 관련된 대립유전자의 멀티플렉스 검출 방법
DK3077539T3 (en) 2013-12-02 2018-11-19 Personal Genome Diagnostics Inc Procedure for evaluating minority variations in a sample
US9476853B2 (en) 2013-12-10 2016-10-25 Life Technologies Corporation System and method for forming microwells
WO2015089243A1 (en) 2013-12-11 2015-06-18 The Regents For Of The University Of California Methods for labeling dna fragments to recontruct physical linkage and phase
CN106232827A (zh) 2014-02-21 2016-12-14 哈佛学院董事及会员团体 变构蛋白的从头设计
ES2741740T3 (es) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Métodos que usan moléculas sintéticas que contienen segmentos de nucleótidos aleatorios
WO2015138343A1 (en) 2014-03-10 2015-09-17 Click Diagnostics, Inc. Cartridge-based thermocycler
EP2921556A1 (de) 2014-03-21 2015-09-23 Lexogen GmbH Anzahlerhaltendes RNA-Analyseverfahren
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
EP3126524B1 (de) 2014-04-04 2020-07-22 Affymetrix, Inc. Verbesserte zusammensetzungen und verfahren für molekularinversionsprobenassays
BE1022048B1 (nl) * 2014-04-11 2016-02-10 Ugentec Bvba Methoden voor fluorescentie data correctie
CA2952013A1 (en) 2014-06-10 2015-12-17 University Of Southern California Usc Discrete elements for 3d microfluidics
GB201411567D0 (en) * 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
DE102014212657B4 (de) * 2014-06-30 2016-03-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. System und Verfahren zur bedarfsgerechten Zuführung von Beleuchtungsenergie an Pflanzen
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
DE102014215352A1 (de) * 2014-08-04 2016-02-04 Berthold Technologies Gmbh & Co. Kg Messgerät und Verfahren zum Bestimmen einer Messgröße
US9982291B2 (en) 2014-08-11 2018-05-29 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
AU2015318823A1 (en) 2014-09-15 2016-03-24 Garvan Institute Of Medical Research Methods for diagnosis, prognosis and monitoring of breast cancer and reagents therefor
ES2895750T3 (es) 2014-09-15 2022-02-22 Abvitro Llc Secuenciación de alto rendimiento de colecciones de nucleótidos
WO2016044416A1 (en) * 2014-09-16 2016-03-24 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated genome engineering and correction in hematopoietic stem cells
US10920279B2 (en) 2014-09-30 2021-02-16 Genetic Technologies Limited Method for modifying a treatment regimen of a human female subject
WO2016060974A1 (en) 2014-10-13 2016-04-21 Life Technologies Corporation Methods, systems, and computer-readable media for accelerated base calling
EP3715455A1 (de) 2014-10-29 2020-09-30 Adaptive Biotechnologies Corp. Hochmultiplexierter gleichzeitiger nachweis von für adaptive gepaarte immunrezeptorheterodimere codierenden nukleinsäuren aus einer vielzahl von proben
CA2967036A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
US9745618B2 (en) 2014-11-19 2017-08-29 Roche Molecular Systems, Inc. Photoblocked probes and methods for sequential detection of nucleic acids
WO2016086029A1 (en) 2014-11-25 2016-06-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US9920381B2 (en) 2014-12-02 2018-03-20 Roche Molecular Systems, Inc. Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
US9623415B2 (en) 2014-12-31 2017-04-18 Click Diagnostics, Inc. Devices and methods for molecular diagnostic testing
US10421993B2 (en) 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
EP4030437A1 (de) 2015-02-17 2022-07-20 Dovetail Genomics, LLC Nukleinsäuresequenzanordnung
ES2858306T3 (es) 2015-02-24 2021-09-30 Adaptive Biotechnologies Corp Método para determinar el estado de HLA mediante secuenciación del repertorio inmunitario
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
WO2016154540A1 (en) 2015-03-26 2016-09-29 Dovetail Genomics Llc Physical linkage preservation in dna storage
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
CA2979726A1 (en) 2015-04-01 2016-10-06 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
EP3288563B1 (de) * 2015-05-01 2019-11-13 Vanderbilt University Überwachung und analyse von nukleinsäurehybridisierungsstatus und amplifikation mit l-dna
CN107969138B (zh) 2015-05-14 2022-04-12 生命科技公司 条形码序列和有关系统与方法
JP6720161B2 (ja) 2015-06-04 2020-07-08 株式会社ミズホメディー 複数の標的核酸の検出キット及びそれを用いる検出方法
AU2016274238B2 (en) * 2015-06-08 2020-04-30 The University Of Chicago Bipyramid-templated synthesis of monodisperse noble metal nanocrystals
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
CA2999888C (en) 2015-09-24 2024-04-09 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
WO2017053902A1 (en) 2015-09-25 2017-03-30 Abvitro Llc High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
US11266730B2 (en) 2015-09-29 2022-03-08 The General Hospital Corporation Methods of treating and diagnosing disease using biomarkers for BCG therapy
JP7300831B2 (ja) 2015-10-19 2023-06-30 ダブテイル ゲノミクス エルエルシー ゲノムアセンブリ、ハプロタイプフェージング、および標的に依存しない核酸検出のための方法
USD799715S1 (en) 2015-10-23 2017-10-10 Gene POC, Inc. Fluidic centripetal device
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
ES2905558T3 (es) 2015-11-13 2022-04-11 Avellino Lab Usa Inc Procedimientos para el tratamiento de las distrofias corneales
WO2017098321A1 (en) 2015-12-11 2017-06-15 Spartan Bioscience Inc. Tube sealing system and methods for nucleic acid amplification
CN108700507B (zh) 2015-12-18 2022-05-27 拜奥法尔防护有限责任公司 固体荧光标准品
CA3012783A1 (en) 2016-01-28 2017-08-03 The University Of Melbourne Methods for assessing risk of developing colorectal cancer
WO2017147279A1 (en) 2016-02-23 2017-08-31 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
BR112018068139B1 (pt) 2016-03-10 2022-12-06 Pioneer Hi-Bred International, Inc Sistema de detecção de produto e amplificação de reação em cadeia da polimerase (pcr) e método de detecção de produto e amplificação de pcr mediada por luz
US10655190B2 (en) 2016-03-11 2020-05-19 Roche Molecular Systems, Inc. Compositions and methods for detection of Zika virus
WO2017185067A1 (en) 2016-04-22 2017-10-26 Click Diagnostics, Inc. Printed circuit board heater for an amplification module
US10619205B2 (en) 2016-05-06 2020-04-14 Life Technologies Corporation Combinatorial barcode sequences, and related systems and methods
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
DK3455356T3 (da) 2016-05-13 2021-11-01 Dovetail Genomics Llc Genfinding af langtrækkende bindingsinformation fra konserverede prøver
ES2929367T3 (es) 2016-05-18 2022-11-28 Hoffmann La Roche Amplificación por PCR ultrarrápida cuantitativa usando un dispositivo basado en electrohumectación
EP3464617B1 (de) 2016-05-27 2021-04-07 Roche Diagnostics GmbH Zusammensetzungen und verfahren zur detektion von trichomonas vaginalis
US10612101B2 (en) 2016-05-27 2020-04-07 Roche Molecular Systems, Inc. Compositions and methods for detection of Mycoplasma genitalium
EP3465506B1 (de) 2016-06-01 2024-04-03 Life Technologies Corporation Verfahren und systeme zum entwerfen von genpanels
US20170356025A1 (en) 2016-06-14 2017-12-14 Roche Molecular Systems, Inc. Internal control probes for improving pcr assay performance
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
RU2691763C2 (ru) * 2016-06-21 2019-06-18 Федеральное государственное бюджетное учреждение науки Институт аналитического приборостроения Российской академии наук (ИАП РАН) Устройство для одновременного контроля в реальном масштабе времени множества амплификаций нуклеиновой кислоты
CN110325652A (zh) 2016-06-29 2019-10-11 易捷仪器诊断股份有限公司 使用流动池检测分子的装置和方法
US10626454B2 (en) 2016-07-27 2020-04-21 SeqMatic, LLC Compositions and methods for nucleic acid amplification and analysis
EP3491152B1 (de) 2016-07-29 2022-06-29 Juno Therapeutics, Inc. Verfahren zur anwesenheit oder abwesenheit bestimmung von replikationskompetente viren
WO2018024562A1 (en) 2016-08-02 2018-02-08 Roche Diagnostics Gmbh Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
JP7221199B2 (ja) 2016-09-15 2023-02-20 エフ． ホフマン－ラ ロシュ エージー． マルチプレックスｐｃｒの実施方法
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10487358B2 (en) 2016-09-23 2019-11-26 Grail, Inc. Methods of preparing and analyzing cell-free nucleic acid sequencing libraries
JP6929354B2 (ja) 2016-09-24 2021-09-01 アブビトロ， エルエルシー 親和性−オリゴヌクレオチドコンジュゲートおよびその使用
US9851345B1 (en) 2016-10-12 2017-12-26 Viasphere, Llc Compositions and methods for disease diagnosis using single cell analysis
DE112016007245T5 (de) 2016-10-17 2019-08-14 Olympus Corporation Verfahren zum Nachweisen von Ziel-Nukleinsäuremolekülen
US20180203974A1 (en) 2016-11-07 2018-07-19 Grail, Inc. Methods of identifying somatic mutational signatures for early cancer detection
US10793923B2 (en) 2016-11-09 2020-10-06 Roche Molecular Systems, Inc. Compositions and methods for detection of BK virus
EP4357455A3 (de) 2016-12-12 2024-07-24 Grail, LLC Verfahren zur markierung und amplifizierung von rna-template-molekülen zur herstellung von sequenzierungsbibliotheken
ES2928184T3 (es) 2016-12-12 2022-11-16 Cepheid Inmuno-PCR integrado y análisis de ácidos nucleicos en un cartucho de reacción automatizado
US20190211377A1 (en) 2016-12-22 2019-07-11 Roche Molecular Systems, Inc. Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile
WO2018119399A1 (en) 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US10793901B2 (en) 2016-12-28 2020-10-06 Roche Molecular Systems, Inc. Reversibly protected nucleotide reagents with high thermal stability
DK3565905T3 (da) 2017-01-04 2022-07-25 Mgi Tech Co Ltd Nukleinsyresekvensering ved hjælp af affinitetsreagenser
CN110382716A (zh) 2017-01-10 2019-10-25 佰隆基因公司 用于减少引物延伸反应中产生的冗余分子条形码的方法和组合物
EP3600403A4 (de) 2017-03-24 2021-01-13 Board of Supervisors of Louisiana State University and Agricultural and Mechanical College Impfstoffstamm vc2 gegen herpes-simplex-virus typ-1 (hsv-1) zur erzeugung einer anti-ehv-1-immunantwort
JP6994513B2 (ja) 2017-03-24 2022-03-04 ジェン－プローブ・インコーポレーテッド 試料中のウイルス病原体を検出するための組成物および方法
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
WO2018200980A1 (en) 2017-04-28 2018-11-01 Avellino Lab Usa, Inc. Methods for detecting alleles associated with keratoconus
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
US20190093155A1 (en) 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
CA3064205A1 (en) 2017-05-26 2018-11-29 Abvitro Llc High-throughput polynucleotide library sequencing and transcriptome analysis
US20180363044A1 (en) 2017-06-14 2018-12-20 Roche Molecular Systems, Inc. Compositions and methods for improving the thermal stability of nucleic acid amplification reagents
US11450121B2 (en) 2017-06-27 2022-09-20 The Regents Of The University Of California Label-free digital brightfield analysis of nucleic acid amplification
US20220225597A1 (en) 2017-06-29 2022-07-21 Juno Therapeutics, Inc. Mouse model for assessing toxicities associated with immunotherapies
US20210155978A1 (en) 2017-07-10 2021-05-27 Gen-Probe Incorporated Analytical systems and methods for nucleic acid amplification using sample assigning parameters
US10760137B2 (en) 2017-07-18 2020-09-01 Roche Molecular Systems, Inc. Compositions and methods for detection of Babesia
CN111246861A (zh) 2017-07-29 2020-06-05 朱诺治疗学股份有限公司 扩增表达重组受体的细胞的试剂
EP3676400A4 (de) 2017-08-31 2021-06-02 Seegene, Inc. Bewertung der leistung von komponenten unter verwendung eines paares von dimerbildenden primern
WO2019046832A1 (en) 2017-09-01 2019-03-07 Juno Therapeutics, Inc. GENE EXPRESSION AND EVALUATION OF RISK OF DEVELOPMENT OF TOXICITY FOLLOWING CELL THERAPY
WO2019055819A1 (en) 2017-09-14 2019-03-21 Grail, Inc. METHODS FOR PREPARING A SEQUENCING LIBRARY FROM SINGLE STRANDED DNA
EP3688191B1 (de) 2017-09-28 2022-03-02 Grail, LLC Anreicherung von kurzen nukleinsäurefragmenten in der erstellung einer sequenzierungsbibliothek
EP3688188A4 (de) 2017-09-29 2021-06-16 Seegene, Inc. Detektion von zielnukleinsäuresequenzen durch pto-spaltung und erweiterungsabhängiger nicht-hybridisierungstest
WO2019063661A1 (en) 2017-09-29 2019-04-04 Roche Diagnostics Gmbh COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
AU2018360801A1 (en) 2017-11-01 2020-05-14 Celgene Corporation Process for producing a T cell composition
US11162130B2 (en) 2017-11-09 2021-11-02 Visby Medical, Inc. Portable molecular diagnostic device and methods for the detection of target viruses
US11078534B2 (en) 2017-11-27 2021-08-03 Roche Molecular Systems, Inc. Photocleavable nucleotide reagents with high stability
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
US11414656B2 (en) 2017-12-15 2022-08-16 Grail, Inc. Methods for enriching for duplex reads in sequencing and error correction
WO2019126803A1 (en) 2017-12-22 2019-06-27 Grail, Inc. Error removal using improved library preparation methods
WO2019135567A1 (en) 2018-01-05 2019-07-11 Seegene, Inc. . Method for determining the presence or absence of m. tuberculosis, m. bovis and m. bovis bcg in a sample
WO2019148169A1 (en) 2018-01-29 2019-08-01 Gen-Probe Incorporated Analytical systems and methods
JP2021511802A (ja) 2018-01-31 2021-05-13 ジュノー セラピューティクス インコーポレイテッド 複製可能ウイルスの存在または非存在を評価するための方法および試薬
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
US20210317515A1 (en) 2018-07-10 2021-10-14 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
EP3836967A4 (de) 2018-07-30 2022-06-15 ReadCoor, LLC Verfahren und systeme zur probenverarbeitung oder -analyse
JP2021533769A (ja) 2018-08-16 2021-12-09 ライフ テクノロジーズ コーポレーション 核酸の増幅のための試薬、混合物、キット、および方法
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
EP3874059A1 (de) 2018-10-29 2021-09-08 Koninklijke Nederlandse Akademie van Wetenschappen Volllängen-rna-sequenzierung einer einzelzelle
US20210393691A1 (en) 2018-11-06 2021-12-23 Juno Therapeutics, Inc. Process for producing genetically engineered t cells
EP3891302A1 (de) 2018-12-03 2021-10-13 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis von candida auris
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
CA3121923A1 (en) 2018-12-18 2020-06-25 Wenying Pan Methods for detecting disease using analysis of rna
CN113272442B (zh) 2019-01-03 2024-03-15 Dna斯克瑞普特公司 寡核苷酸的集合的一锅合成
JP2022525322A (ja) 2019-03-14 2022-05-12 インシリクサ， インコーポレイテッド 時間ゲート蛍光ベースの検出のための方法およびシステム
AU2020257238A1 (en) 2019-04-17 2021-12-02 Alpine Immune Sciences, Inc. Methods and uses of variant ICOS Ligand (ICOSL) fusion proteins
WO2020223185A1 (en) * 2019-05-01 2020-11-05 Luminex Corporation Apparatus and methods for thermal cycling of sample
WO2020221915A1 (en) 2019-05-02 2020-11-05 F. Hoffmann-La Roche Ag UTILIZATION OF dITP FOR PREFERENTIAL/SELECTIVE AMPLIFICATION OF RNA VERSUS DNA TARGETS BASED ON STRAND-SEPARATION TEMPERATURE
EP3966351A1 (de) 2019-05-07 2022-03-16 F. Hoffmann-La Roche AG Zusammensetzungen und verfahren zum nachweis von neisseria gonorroheae
CN114269371A (zh) 2019-06-12 2022-04-01 朱诺治疗学股份有限公司 细胞介导的细胞毒性疗法与促存活bcl2家族蛋白的抑制剂的组合疗法
CN114341638A (zh) 2019-06-27 2022-04-12 多弗泰尔基因组学有限责任公司 用于邻近连接的方法和组合物
WO2021013972A1 (en) 2019-07-25 2021-01-28 F. Hoffmann-La Roche Ag Compositions and methods for detection of epstein barr virus (ebv)
CN114555112A (zh) 2019-08-22 2022-05-27 朱诺治疗学股份有限公司 T细胞疗法和zeste增强子同源物2(ezh2)抑制剂的组合疗法及相关方法
US11739382B2 (en) 2019-08-27 2023-08-29 Roche Molecular Systems, Inc. Compositions and methods for amplification and detection of hepatitis B virus RNA, including HBV RNA transcribed from cccDNA
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
US20230050863A1 (en) 2019-12-09 2023-02-16 Roche Molecular Systems, Inc. Dicationic fluorescent dyes
JP2023508100A (ja) 2019-12-27 2023-02-28 エフ． ホフマン－ラ ロシュ エージー． メチシリン耐性黄色ブドウ球菌を検出するための組成物および方法
WO2021138544A1 (en) 2020-01-03 2021-07-08 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
WO2021140173A1 (en) 2020-01-10 2021-07-15 Biouniversa S.R.L. Methods and uses for treating fibrotic solid tumors with bags inhibitors
CN115335511A (zh) 2020-01-13 2022-11-11 艾斯本神经科学公司 使神经细胞分化的方法及相关组合物和使用方法
JP2023516472A (ja) 2020-03-09 2023-04-19 エフ． ホフマン－ラ ロシュ アーゲー 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）、インフルエンザａ及びインフルエンザｂを検出するための組成物及び方法
US20210332448A1 (en) 2020-03-09 2021-10-28 Janssen Pharmaceutica Nv Compositions and methods for quantifying integration of recombinant vector nucleic acid
CN115427586A (zh) 2020-03-18 2022-12-02 深圳华大智造科技股份有限公司 大规模平行测序中的恢复阶段
CN115835873A (zh) 2020-05-13 2023-03-21 朱诺治疗学股份有限公司 用于产生表达重组受体的供体分批细胞的方法
US10941453B1 (en) 2020-05-20 2021-03-09 Paragon Genomics, Inc. High throughput detection of pathogen RNA in clinical specimens
EP4158067A1 (de) 2020-05-27 2023-04-05 Genetic Technologies Limited Verfahren zur beurteilung des risikos der entwicklung einer schweren reaktion auf coronavirusinfektion
CN116234558A (zh) 2020-06-26 2023-06-06 朱诺治疗学有限公司 条件性地表达重组受体的工程化t细胞、相关多核苷酸和方法
US20230129884A1 (en) * 2020-07-20 2023-04-27 Photothermal Spectroscopy Corp. Fluorescence enhanced photothermal infrared spectroscopy and confocal fluorescence imaging
US20240052391A1 (en) 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
CN112461806B (zh) * 2020-11-18 2022-02-11 厦门大学 基于智能手机的荧光光谱检测方法
JP2023552546A (ja) 2020-12-04 2023-12-18 エフ． ホフマン－ラ ロシュ アーゲー マラリアを検出するための組成物および方法
JP7209980B2 (ja) 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
CN116601308A (zh) 2020-12-22 2023-08-15 豪夫迈·罗氏有限公司 使用大斯托克斯位移荧光染料进行多重实时pcr的方法
US20220205020A1 (en) 2020-12-30 2022-06-30 Roche Molecular Systems, Inc. Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis
CN116806268A (zh) 2021-02-05 2023-09-26 豪夫迈·罗氏有限公司 用于检测人副流感病毒1-4(hpiv 1-4)的组合物和方法
US20220290221A1 (en) 2021-03-15 2022-09-15 Roche Molecular Systems, Inc. Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations
CN117858960A (zh) 2021-05-06 2024-04-09 豪夫迈·罗氏有限公司 用于通过双靶标测定来检测丁型肝炎病毒的组合物和方法
WO2023283452A2 (en) 2021-07-09 2023-01-12 Cepheid High-level multiplexing reaction vessel, reagent spotting device and associated methods
WO2023004371A1 (en) 2021-07-21 2023-01-26 Aspen Neuroscience, Inc. Methods of differentiating neural cells and predicting engraftment thereof and related compositions
WO2023043280A1 (ko) 2021-09-17 2023-03-23 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
EP4412765A1 (de) 2021-10-06 2024-08-14 Bayer Aktiengesellschaft Integriertes system für chemische, biochemische oder molekulare biologische reaktionen in einer mikroplatte
US20230121442A1 (en) 2021-10-06 2023-04-20 Johnson & Johnson Consumer Inc. Method of Quantifying Product Impact on Human Microbiome
WO2023079032A1 (en) 2021-11-05 2023-05-11 F. Hoffmann-La Roche Ag Compositions and methods for detection of malaria
CN118339315A (zh) 2021-11-22 2024-07-12 豪夫迈·罗氏有限公司 用于检测与多药耐药性相关的vanA和/或vanB基因的组合物和方法
AU2022402153A1 (en) 2021-12-03 2024-06-13 Medicinal Genomics Corporation Psilocybe assay
WO2023146243A1 (ko) 2022-01-26 2023-08-03 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법
US20230377685A1 (en) 2022-04-15 2023-11-23 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
US11680293B1 (en) 2022-04-21 2023-06-20 Paragon Genomics, Inc. Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules
WO2023230581A1 (en) 2022-05-25 2023-11-30 Celgene Corporation Methods of manufacturing t cell therapies
WO2024003114A1 (en) 2022-06-29 2024-01-04 Actome Gmbh Detection of biomolecules in single cells
WO2024003332A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Controlling for tagmentation sequencing library insert size using archaeal histone-like proteins
WO2024003260A1 (en) 2022-06-30 2024-01-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays
WO2024042042A1 (en) 2022-08-24 2024-02-29 F. Hoffmann-La Roche Ag Compositions and methods for detecting monkeypox virus
WO2024059493A1 (en) 2022-09-13 2024-03-21 Medicinal Genomics Corporation Psilocybe assay
WO2024062208A1 (en) 2022-09-20 2024-03-28 Cost-Bry Pty Ltd (trading as BiomeBank) Compositions and methods for reducing endogenous sulphide in inflammatory bowel diseases
WO2024141442A1 (en) 2022-12-25 2024-07-04 F. Hoffmann-La Roche Ag Compositions and methods for detecting carbapenem resistant acinetobacter calcoaceticus-baumannii (crab)
WO2024156896A2 (en) 2023-01-26 2024-08-02 Dna Script Enzymatic synthesis of polynucleotide probes
WO2024175749A1 (en) 2023-02-25 2024-08-29 F. Hoffmann-La Roche Ag Compositions and methods for candida species detection

Family Cites Families (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1006767A (en) 1910-01-19 1911-10-24 Gen Electric Electric hair-drier.
US1456005A (en) 1922-07-24 1923-05-22 Harris Jack Incubator
US2379474A (en) 1943-11-10 1945-07-03 Bramson Maurice Heating cabinet, incubator, and the like
US3219416A (en) 1962-10-30 1965-11-23 Scientific Industries Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material
US3556659A (en) 1966-02-03 1971-01-19 Applied Physics Corp Laser-excited raman spectrometer
US3616264A (en) 1969-06-30 1971-10-26 Beckman Instruments Inc Temperature-controlled discrete sample analyzer
US3718133A (en) 1971-01-12 1973-02-27 Damon Corp Container unit for liquid samples
US4038055A (en) 1975-10-10 1977-07-26 Block Engineering, Inc. Gas chromatograph for continuous operation with infrared spectrometer
US4168017A (en) 1977-04-05 1979-09-18 Anderwald Cecil E Container means preventing accidental use by children
AU528259B2 (en) 1978-09-14 1983-04-21 Union Carbide Corp Method and apparatus for assaying liquid materials
IT1101295B (it) 1978-11-22 1985-09-28 Erba Strumentazione Camera gas-cromatografica
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4420679A (en) 1982-02-26 1983-12-13 Delta Associates, Inc. Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air
US4468423A (en) 1982-11-17 1984-08-28 Arlie Hall Insulating cell element and structures composed thereof
US4468426A (en) * 1982-11-24 1984-08-28 E. I. Du Pont De Nemours And Company Adherable reinforced poly(ethylene terephthalate) composite sheet
US4481405A (en) 1983-04-27 1984-11-06 Malick Franklin S Cooking appliance
US4599169A (en) 1984-02-28 1986-07-08 Varian Associates, Inc. Heating and cooling apparatus for chromatography column
US4701415A (en) 1984-03-02 1987-10-20 Mallinckrodt, Inc. Controlled atmosphere enclosure
JPS60212986A (ja) 1984-04-06 1985-10-25 日本特殊陶業株式会社 直流電圧印加用セラミツクヒ−タ−
JPS60241884A (ja) 1984-05-15 1985-11-30 Tokyo Daigaku 自動サイクリング反応装置およびこれを用いる自動分析装置
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5333675C1 (en) 1986-02-25 2001-05-01 Perkin Elmer Corp Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
JPS6212986A (ja) 1985-07-10 1987-01-21 Hitachi Ltd 磁気バブルメモリデバイスのパツケ−ジ
US4740472A (en) 1985-08-05 1988-04-26 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4675300A (en) 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
CA1273552A (en) * 1985-12-23 1990-09-04 Michael J. Heller Fluorescent stokes shift probes for polynucleotide hybridization assays
EP0232967B1 (de) * 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CA1339653C (en) 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4708782A (en) 1986-09-15 1987-11-24 Sepragen Corporation Chromatography column-electrophoresis system
US4684465A (en) 1986-10-10 1987-08-04 Combustion Engineering, Inc. Supercritical fluid chromatograph with pneumatically controlled pump
US4902624A (en) 1987-11-23 1990-02-20 Eastman Kodak Company Temperature cycling cuvette
DE3808942A1 (de) 1988-03-17 1989-09-28 Bio Med Gmbh Ges Fuer Biotechn Inkubator, insbes. fuer die polymerase-ketten-methode
GB8807297D0 (en) 1988-03-26 1988-04-27 Dean P D G Intelligent heating block
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US4865986A (en) 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
US5169511A (en) 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5536648A (en) 1988-12-09 1996-07-16 Amrad Corporation Limited Amplified DNA assay using a double stranded DNA binding protein
AU638234B2 (en) 1989-02-08 1993-06-24 City Of Hope Apparatus for peptide sequencing
ATE112181T1 (de) 1989-06-20 1994-10-15 Claudio Bonini Prüfröhrchen mit einer linsenförmigen aussenfläche, insbesondere für automatische klinische analysen.
US5449621A (en) 1989-07-31 1995-09-12 Biotope, Inc. Method for measuring specific binding assays
US5346672A (en) 1989-11-17 1994-09-13 Gene Tec Corporation Devices for containing biological specimens for thermal processing
US5075556A (en) * 1989-12-01 1991-12-24 Technicon Instruments Corporation Acridine orange derivatives and their use in the quantitation of reticulocytes in whole blood
US5169521A (en) 1989-12-13 1992-12-08 The United States Of America As Represented By The Department Of Health And Human Services Apparatus for countercurrent chromatography separations
US5173163A (en) 1990-01-11 1992-12-22 Isco, Inc. Capillary electrophoresis technique
US5141621A (en) 1990-01-26 1992-08-25 The Board Of Trustees Of The Leland Stanford Junior University Capillary electrophoresis injection device and method
US5207987A (en) * 1990-05-21 1993-05-04 Pb Diagnostic Systems Inc. Temperature controlled chamber for diagnostic analyzer
DE69104775T2 (de) 1990-05-29 1995-06-01 Waters Investments Ltd Verfahren und Vorrichtung zum Durchführen der Kapillarelektroforese.
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US5187084A (en) 1990-06-22 1993-02-16 The Dow Chemical Company Automatic air temperature cycler and method of use in polymerose chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5269900A (en) 1990-09-13 1993-12-14 University Of North Carolina At Chapel Hill Method and device for high speed separation of complex molecules
EP0487218B1 (de) * 1990-10-31 1997-12-29 Tosoh Corporation Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5131998A (en) 1990-11-13 1992-07-21 The University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
US5240577A (en) 1990-11-13 1993-08-31 University Of North Carolina At Chapel Hill Two-dimensional high-performance liquid chromatography/capillary electrophoresis
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5380489A (en) 1992-02-18 1995-01-10 Eastman Kodak Company Element and method for nucleic acid amplification and detection using adhered probes
WO1992017259A1 (en) 1991-03-28 1992-10-15 Perseptive Biosystems, Inc. On-line product identification in a chromatography effluent by subtraction
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
WO1992020778A1 (en) 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control
US5340715A (en) 1991-06-07 1994-08-23 Ciba Corning Diagnostics Corp. Multiple surface evanescent wave sensor with a reference
US5316913A (en) 1991-09-06 1994-05-31 Stanford University Neutrophil LECAM-1 as indicator of neutrophil activation
US5114551A (en) 1991-09-30 1992-05-19 Bio-Rad Laboratories, Inc. Multi-point detection method for electrophoresis and chromatography in capillaries
US5116471A (en) 1991-10-04 1992-05-26 Varian Associates, Inc. System and method for improving sample concentration in capillary electrophoresis
ES2159282T3 (es) * 1991-11-07 2001-10-01 Nanotronics Inc Hibridacion de polinucleotidos conjugados con cromoforas y fluoroforos con el fin de generar sistemas de transferencia de energia de donador a donador.
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
DE4234086A1 (de) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
ES2082256T3 (es) 1992-03-23 1996-03-16 Hoffmann La Roche Metodo de deteccion del adn.
DE69331067T2 (de) 1992-04-06 2002-07-11 Abbott Laboratories, Abbott Park Verfahren und vorrichtung zum nachweis von nukleinsäure oder analysen unter verwendung der internen totalreflexion
JPH0634546A (ja) 1992-07-17 1994-02-08 Tosoh Corp 蛍光検出器
EP0609431B1 (de) 1992-08-24 1997-10-08 Dade MicroScan Inc. Abdichtbarer behälter zum konservieren und behandeln analytischer proben
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5593840A (en) 1993-01-27 1997-01-14 Oncor, Inc. Amplification of nucleic acid sequences
US5364790A (en) 1993-02-16 1994-11-15 The Perkin-Elmer Corporation In situ PCR amplification system
US5798215A (en) 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US6027884A (en) * 1993-06-17 2000-02-22 The Research Foundation Of The State University Of New York Thermodynamics, design, and use of nucleic acid sequences
JP3598123B2 (ja) 1993-07-15 2004-12-08 浜松ホトニクス株式会社 核酸の変性検出装置
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
CA2129787A1 (en) 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
EP0643140B1 (de) 1993-09-13 2001-10-31 Canon Kabushiki Kaisha Bestimmung von Nukleinsäuren durch PCR, Messung der Anzahl von mikrobiellen Zellen, Genen oder Genkopien durch PCR und Kit zu ihrer Verwendung
US5415839A (en) 1993-10-21 1995-05-16 Abbott Laboratories Apparatus and method for amplifying and detecting target nucleic acids
US5804380A (en) 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
EP2423322A1 (de) 1993-11-12 2012-02-29 PHRI Properties, Inc. Hybridisierungsprobe zur Nukleinsäuredetektion, universelle Stammstrukturen, Methoden und Reagentiensätze
US5863726A (en) 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
GB9401535D0 (en) 1994-01-27 1994-03-23 Steer Rupert P Timing devices
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
DE69532953T8 (de) 1994-02-01 2005-10-06 IGENE, Inc. (n.d.Ges.d.Staates Delaware), Menlo Park Molekularanalysator und verfahren zu seiner verwendung
JP3265113B2 (ja) * 1994-03-04 2002-03-11 三菱製紙株式会社 インクジェット記録シート
CA2143365A1 (en) 1994-03-14 1995-09-15 Hugh V. Cottingham Nucleic acid amplification method and apparatus
WO1995030139A1 (en) * 1994-04-29 1995-11-09 Perkin-Elmer Corporation System for real time detection of nucleic acid amplification products
DE69533660T2 (de) * 1994-04-29 2005-11-10 Johnson & Johnson Clinical Diagnostics, Inc. Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits
BR9408578A (pt) * 1994-05-23 1997-08-19 Biotronics Corp Método para detecção de um ácido nucléico alvo
US5766889A (en) 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
DE69527125T2 (de) * 1994-11-09 2003-02-27 Hitachi, Ltd. Verfahren und Vorrichtung zur DNS Analyse
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
JPH11506605A (ja) * 1995-06-07 1999-06-15 アボツト・ラボラトリーズ 増幅反応におけるバックグラウンドを減少させるためにプローブをマスキングする方法
WO1996041864A1 (en) 1995-06-13 1996-12-27 The Regents Of The University Of California Diode laser heated micro-reaction chamber with sample detection means
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
CA2658290C (en) * 1996-06-04 2012-04-10 University Of Utah Research Foundation Monitoring hybridization during pcr using fluorescent dye specific to double-stranded dna
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
EP0822261B1 (de) * 1996-07-29 2002-07-03 Academia Sinica Verfahren zur schnellen antimikrobiellen Empfindlichkeitsnachweis
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6153411A (en) * 1998-10-30 2000-11-28 American Water Works Company, Inc. Methods and kits for detection of Cryptosporidium parvum using immunomagnetic separation and amplification

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