ES2131699T3 - Procedimiento mejorado de cuantificacion del acido nucleico. - Google Patents

Procedimiento mejorado de cuantificacion del acido nucleico.

Info

Publication number
ES2131699T3
ES2131699T3 ES94924750T ES94924750T ES2131699T3 ES 2131699 T3 ES2131699 T3 ES 2131699T3 ES 94924750 T ES94924750 T ES 94924750T ES 94924750 T ES94924750 T ES 94924750T ES 2131699 T3 ES2131699 T3 ES 2131699T3
Authority
ES
Spain
Prior art keywords
nucleic acid
analyte
constructs
construct
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94924750T
Other languages
English (en)
Inventor
Marinus Gerardus Joh Beuningen
Tim Kievits
Thomas Augustinus Maria Beumer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo Nobel NV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Application granted granted Critical
Publication of ES2131699T3 publication Critical patent/ES2131699T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

EL PRESENTE INVENTO SE RELACIONA CON UN METODO MEJORADO PARA LA CUANTIFICACION DE ACIDO NUCLEICO, QUE PUEDE PONERSE EN PRACTICA CON UNA CANTIDAD MINIMA DE REACCIONES DE AMPLIFICACION DE ACIDO NUCLEICO. ESTE METODO PARA CUANTIFICAR EL ACIDO NUCLEICO ANALIZADO EN UNA MUESTRA COMPRENDE LOS PASOS DE: AÑADIDO A LA MUESTRA DE CANTIDADES DIFERENTES DE ESTRUCTURAS DE ACIDO NUCLEICO, SIENDO DISTINGUIBLE CADA ESTRUCTURA DEL ACIDO ANALIZADO Y SIENDO CAPAZ DE SER COAMPLIFICADO CON EL ACIDO NUCLEICO ANALIZADO; SOMETIMIENTO DE LA MUESTRA A UN PROCEDIMIENTO DE AMPLIFICACION DE ACIDO NUCLEICO, UTILIZANDO REACTIVOS DE AMPLIFICACION CAPACES DE REACCIONAR CON EL ACIDO NUCLEICO ANALIZADO Y LAS ESTRUCTURAS DE ACIDO NUCLEICO; DETECCION DE CANTIDADES RELATIVAS DE AMPLIFICADOS DERIVADOS DEL ACIDO NUCLEICO ANALIZADO Y CADA ESTRUCTURA DE ACIDO NUCLEICO; CALCULO DE LA CANTIDAD DE ACIDO NUCLEICO ANALIZADO A PARTIR DE TALES CANTIDADES RELATIVAS. CADA ESTRUCTURA DE ACIDO NUCLEICO ES DISTINTA; LAS ESTRUCTURAS DE ACIDO NUCLEICO PUEDEN DISTINGUIRSE UNA DE OTRA Y DEL ACIDO NUCLEICO ANALIZADO. LAS ESTRUCTURAS DE ACIDO NUCLEICO SE PARECEN ENTRE SI, Y EL ACIDO NUCLEICO ANALIZADO, EN LA MEDIDA EN QUE TODOS SON CAPACES DE REACCIONAR CON LOS MISMOS REACTIVOS DE AMPLIFICACION.
ES94924750T 1993-07-09 1994-07-08 Procedimiento mejorado de cuantificacion del acido nucleico. Expired - Lifetime ES2131699T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93202015 1993-07-09
EP93203424 1993-12-06

Publications (1)

Publication Number Publication Date
ES2131699T3 true ES2131699T3 (es) 1999-08-01

Family

ID=26133903

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94924750T Expired - Lifetime ES2131699T3 (es) 1993-07-09 1994-07-08 Procedimiento mejorado de cuantificacion del acido nucleico.

Country Status (10)

Country Link
JP (1) JP3594598B2 (es)
KR (1) KR100312800B1 (es)
AT (1) ATE178946T1 (es)
AU (1) AU668659B2 (es)
CA (1) CA2143857C (es)
DE (1) DE69417863T2 (es)
DK (1) DK0662156T3 (es)
ES (1) ES2131699T3 (es)
FI (1) FI112094B (es)
WO (1) WO1995002067A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0722508B1 (en) * 1993-09-22 2003-04-02 Igen International, Inc. Self-sustained sequence replication electrochemiluminescent nucleic acid assay
CA2159044A1 (en) * 1994-09-26 1996-03-27 Falko-Guenter Falkner Method of quantitating nucleic acids
CA2223241C (en) * 1995-06-07 2009-11-17 Gen-Probe Incorporated Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US5795722A (en) * 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5939262A (en) * 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US5677124A (en) * 1996-07-03 1997-10-14 Ambion, Inc. Ribonuclease resistant viral RNA standards
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
FR2765590B1 (fr) * 1997-07-01 2002-08-09 Pasteur Institut Methode de semi-quantification d'alleles
AU4515699A (en) * 1998-06-26 2000-01-17 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
EP1013776A1 (en) * 1998-12-22 2000-06-28 Universiteit van Amsterdam A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
DK1238100T3 (da) * 1999-07-23 2006-01-23 Gen Probe Inc Polynucleotid-amplifikationsfremgangsmåde
AT409383B (de) * 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion und quantifizierung von nukleinsäuren in einer probe
US7790368B1 (en) 2000-09-22 2010-09-07 Hitachi, Ltd. Method for analyzing nucleic acid
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Ｄｎａ定量方法、及び遺伝子解析方法
JP2011062119A (ja) 2009-09-16 2011-03-31 Seiko Epson Corp 生体試料定量用チップ
AU2012221740B2 (en) 2011-02-25 2016-11-17 Novartis Ag Exogenous internal positive control
KR102108855B1 (ko) 2017-10-30 2020-05-12 한국표준과학연구원 안정동위원소 표지 핵산을 내부표준물질로 활용하는 핵산 정량 방법 및 이의 용도

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
DE69233285T2 (de) * 1991-08-02 2004-11-25 bioMérieux B.V. Quantifikation von Nukleinsäuren
FR2683827B1 (fr) * 1991-11-15 1994-03-04 Institut Nal Sante Recherc Medic Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.

Also Published As

Publication number Publication date
FI112094B (fi) 2003-10-31
CA2143857A1 (en) 1995-01-19
KR100312800B1 (ko) 2002-02-28
AU7492894A (en) 1995-02-06
CA2143857C (en) 2005-09-20
FI951082A (fi) 1995-03-08
ATE178946T1 (de) 1999-04-15
AU668659B2 (en) 1996-05-09
JPH08501222A (ja) 1996-02-13
DE69417863D1 (de) 1999-05-20
DE69417863T2 (de) 1999-10-07
FI951082A0 (fi) 1995-03-08
DK0662156T3 (da) 1999-10-25
WO1995002067A1 (en) 1995-01-19
JP3594598B2 (ja) 2004-12-02

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