DE69936642D1 - Verfahren unter verwendung ungleicher primer-konzentrationen zur herstellung von nukleinsäure-amplifikaten - Google Patents

Verfahren unter verwendung ungleicher primer-konzentrationen zur herstellung von nukleinsäure-amplifikaten

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Publication number
DE69936642D1
DE69936642D1 DE69936642T DE69936642T DE69936642D1 DE 69936642 D1 DE69936642 D1 DE 69936642D1 DE 69936642 T DE69936642 T DE 69936642T DE 69936642 T DE69936642 T DE 69936642T DE 69936642 D1 DE69936642 D1 DE 69936642D1
Authority
DE
Germany
Prior art keywords
primer
target sequence
manufacture
nucleic acid
test sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69936642T
Other languages
English (en)
Other versions
DE69936642T2 (de
Inventor
John A Salituro
John L Carrino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of DE69936642D1 publication Critical patent/DE69936642D1/de
Application granted granted Critical
Publication of DE69936642T2 publication Critical patent/DE69936642T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69936642T 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer-konzentrationen zur herstellung von nukleinsäure-amplifikaten Expired - Lifetime DE69936642T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US79675 1998-05-15
US09/079,675 US6391544B1 (en) 1998-05-15 1998-05-15 Method for using unequal primer concentrations for generating nucleic acid amplification products
PCT/US1999/010271 WO1999060159A2 (en) 1998-05-15 1999-05-11 Method for using unequal primer concentrations for generating nucleic acid amplification products

Publications (2)

Publication Number Publication Date
DE69936642D1 true DE69936642D1 (de) 2007-09-06
DE69936642T2 DE69936642T2 (de) 2008-05-21

Family

ID=22152084

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69936642T Expired - Lifetime DE69936642T2 (de) 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer-konzentrationen zur herstellung von nukleinsäure-amplifikaten

Country Status (7)

Country Link
US (2) US6391544B1 (de)
EP (1) EP1076721B1 (de)
JP (1) JP4634608B2 (de)
AT (1) ATE368129T1 (de)
CA (1) CA2332518C (de)
DE (1) DE69936642T2 (de)
WO (1) WO1999060159A2 (de)

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US7115365B2 (en) * 1998-12-07 2006-10-03 Olympus Optical Co., Ltd. Method of analyzing a target nucleic acid
DE60041489D1 (de) * 1999-07-09 2009-03-19 Gen Probe Inc Nachweis von hiv-1 durch amplifizierung von nukleinsäuren
US6852491B2 (en) * 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
CN1910289B (zh) * 2004-01-07 2012-05-23 日立化成研究中心公司 用于检测hiv的引物和探针
AU2005216549A1 (en) * 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20090068650A1 (en) * 2005-02-11 2009-03-12 Southern Illinois University Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
US20130065233A1 (en) * 2010-03-03 2013-03-14 Zymo Research Corporation Detection of dna methylation
GB2512213B (en) 2010-10-08 2015-02-11 Harvard College High-throughput single cell barcoding
WO2012048340A2 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput immune sequencing
ES2576927T3 (es) 2010-10-22 2016-07-12 T2 Biosystems, Inc. Sistemas de RMN y métodos para la detección rápida de analitos
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
WO2013055995A2 (en) 2011-10-14 2013-04-18 President And Fellows Of Harvard College Sequencing by structure assembly
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013158281A1 (en) 2012-04-20 2013-10-24 T2 Biosystems, Inc. Compositions and methods for detection of candida species
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
CN108875312A (zh) 2012-07-19 2018-11-23 哈佛大学校长及研究员协会 利用核酸存储信息的方法
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
EP3578666A1 (de) 2013-03-12 2019-12-11 President and Fellows of Harvard College Verfahren zur erzeugung einer dreidimensionalen nukleinsäure mit matrix
US9765404B2 (en) 2013-09-04 2017-09-19 Dow Agrosciences Llc Rapid assay for identifying transformants having targeted donor insertion
ES2911684T3 (es) 2014-02-21 2022-05-20 Harvard College Diseño de novo de proteínas alostéricas
EP3129493B1 (de) 2014-04-09 2021-07-07 The Scripps Research Institute Import von unnatürlichen oder modifizierten nukleosid-triphosphaten in zellen mittels nukleinsäure-triphosphattransportern
CA2967036A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
US10801060B2 (en) 2015-02-24 2020-10-13 Zymo Research Corporation Assays to determine DNA methylation and DNA methylation markers of cancer
US11028443B2 (en) 2015-08-31 2021-06-08 Showa Denko Materials Co., Ltd. Molecular methods for assessing urothelial disease
EP3405479A4 (de) 2016-01-21 2019-08-21 T2 Biosystems, Inc. Nmr-verfahren und -systeme zur schnellen detektion von bakterien
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
AU2018300069A1 (en) 2017-07-11 2020-02-27 Synthorx, Inc. Incorporation of unnatural nucleotides and methods thereof
WO2019028425A1 (en) 2017-08-03 2019-02-07 Synthorx, Inc. CONJUGATES OF CYTOKINE FOR THE TREATMENT OF AUTOIMMUNE DISEASES
EP3613855A1 (de) 2018-08-23 2020-02-26 Clariant Produkte (Deutschland) GmbH Verfahren zur herstellung einer nukleinsäurenbibliothek
US20210388431A1 (en) * 2018-10-29 2021-12-16 Igor V. Kutyavin Thermokinetically balanced isothermal amplification of nucleic acid sequences
MX2021009259A (es) 2019-02-06 2021-08-24 Synthorx Inc Conjugados de il-2 y metodos de uso del mismo.
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5629158A (en) 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
CA2024978A1 (en) 1989-09-22 1991-03-23 Annie L. Wu Nucleic acid detection method using unequal primer concentrations in polymerase chain reaction
EP0516753A4 (en) * 1990-02-26 1994-05-18 Us Health A method for the fluorescent detection of a dna sequence in real time
US5833976A (en) * 1991-08-06 1998-11-10 Schering Corporation Use of interleukin-10 (IL-10) to treat endotoxin- or superantigen-induced toxicity
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US5464746A (en) 1991-12-17 1995-11-07 Abbott Laboratories Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives
US5424414A (en) 1991-12-17 1995-06-13 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
NZ263779A (en) 1993-04-16 1997-03-24 F B Investments Pty Ltd Former Method for random amplification of dna
US5763174A (en) * 1994-02-17 1998-06-09 The Wistar Institute Of Anatomy & Biology RNA editing enzyme and methods of use thereof
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
DE19808534A1 (de) * 1998-02-28 1999-09-02 Roche Diagnostics Gmbh Verfahren zum Nachweis hochkonzentrierter Nukleinsäuren
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products

Also Published As

Publication number Publication date
US20020160406A1 (en) 2002-10-31
JP4634608B2 (ja) 2011-02-16
US6391544B1 (en) 2002-05-21
EP1076721A2 (de) 2001-02-21
US20020045163A1 (en) 2002-04-18
DE69936642T2 (de) 2008-05-21
WO1999060159A3 (en) 2000-02-24
ATE368129T1 (de) 2007-08-15
US7037688B2 (en) 2006-05-02
EP1076721B1 (de) 2007-07-25
CA2332518C (en) 2009-07-14
WO1999060159A2 (en) 1999-11-25
JP2002515261A (ja) 2002-05-28
CA2332518A1 (en) 1999-11-25

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