DK0522884T3 - Fremgangsmåde til bekæmpelse af kontaminering af nukleinsyreamplifikationsreaktioner - Google Patents

Fremgangsmåde til bekæmpelse af kontaminering af nukleinsyreamplifikationsreaktioner

Info

Publication number
DK0522884T3
DK0522884T3 DK92306396T DK92306396T DK0522884T3 DK 0522884 T3 DK0522884 T3 DK 0522884T3 DK 92306396 T DK92306396 T DK 92306396T DK 92306396 T DK92306396 T DK 92306396T DK 0522884 T3 DK0522884 T3 DK 0522884T3
Authority
DK
Denmark
Prior art keywords
exo
sample
nucleic acid
acid amplification
incorporated
Prior art date
Application number
DK92306396T
Other languages
English (en)
Inventor
James L Hartley
Mark Berninger
Original Assignee
Life Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Inc filed Critical Life Technologies Inc
Application granted granted Critical
Publication of DK0522884T3 publication Critical patent/DK0522884T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DK92306396T 1991-07-12 1992-07-13 Fremgangsmåde til bekæmpelse af kontaminering af nukleinsyreamplifikationsreaktioner DK0522884T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US72887491A 1991-07-12 1991-07-12

Publications (1)

Publication Number Publication Date
DK0522884T3 true DK0522884T3 (da) 1998-07-20

Family

ID=24928620

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92306396T DK0522884T3 (da) 1991-07-12 1992-07-13 Fremgangsmåde til bekæmpelse af kontaminering af nukleinsyreamplifikationsreaktioner

Country Status (8)

Country Link
EP (1) EP0522884B1 (da)
JP (1) JPH0811070B2 (da)
AT (1) ATE159764T1 (da)
CA (1) CA2073298C (da)
DE (1) DE69222897T2 (da)
DK (1) DK0522884T3 (da)
ES (1) ES2109983T3 (da)
GR (1) GR3025964T3 (da)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
JPH09505475A (ja) * 1993-11-23 1997-06-03 チバ コーニング ダイアグノスティクス コーポレイション 核酸増幅反応における不純物混入の制御プロセスにおけるアンチセンスオリゴマーの使用
JP3517954B2 (ja) * 1994-06-13 2004-04-12 株式会社デンソー 車両のアンチスキッド制御装置
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates
ZA956776B (en) 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
DE19736062A1 (de) * 1997-08-20 1999-02-25 Boehringer Mannheim Gmbh Reduktion von Kreuzkontaminationen bei Nukleinsäureamplifikationen
US8669061B2 (en) * 2008-06-26 2014-03-11 Roche Molecular Systems, Inc. Method for the prevention of carryover contamination in nucleic acid amplification technologies
PT3690060T (pt) * 2014-10-08 2022-01-27 Univ Cornell Método de identificação e quantificação relativa da expressão de sequências de ácidos nucleicos, variantes de splicing, translocação, número de cópias ou alterações de metilação utilizando reações combinadas de nuclease, ligação e polimerase com prevenção de arraste
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
CN113293200B (zh) * 2021-05-28 2022-03-04 北京金匙基因科技有限公司 一种降低或消除二代测序中扩增产物污染的方法及应用
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
FR2649122B1 (fr) * 1989-07-03 1991-10-18 Pasteur Institut Perfectionnements apportes aux techniques d'amplification par reaction de polymerisation en chaine (pcr)
DE69024286T2 (de) * 1989-09-01 1996-05-30 Life Technologies Inc Verfahren zur Überprüfung der Kontamination von oligonukleotidabhängigen Nukleinsäure-Amplifikationsreaktionen

Also Published As

Publication number Publication date
GR3025964T3 (en) 1998-04-30
DE69222897T2 (de) 1998-10-01
CA2073298C (en) 2007-04-17
ATE159764T1 (de) 1997-11-15
EP0522884A1 (en) 1993-01-13
EP0522884B1 (en) 1997-10-29
JPH0690755A (ja) 1994-04-05
DE69222897D1 (de) 1997-12-04
ES2109983T3 (es) 1998-02-01
CA2073298A1 (en) 1993-01-13
JPH0811070B2 (ja) 1996-02-07

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