DE602004021235D1 - Verfahren, kits und reagenzien zur nukleinsäuresequenzierung - Google Patents

Verfahren, kits und reagenzien zur nukleinsäuresequenzierung

Info

Publication number
DE602004021235D1
DE602004021235D1 DE602004021235T DE602004021235T DE602004021235D1 DE 602004021235 D1 DE602004021235 D1 DE 602004021235D1 DE 602004021235 T DE602004021235 T DE 602004021235T DE 602004021235 T DE602004021235 T DE 602004021235T DE 602004021235 D1 DE602004021235 D1 DE 602004021235D1
Authority
DE
Germany
Prior art keywords
nucleic acid
kits
reagents
acid sequencing
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004021235T
Other languages
English (en)
Inventor
Mark J Hoser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Geneform Technologies Ltd
Original Assignee
Geneform Technologies Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0303964A external-priority patent/GB0303964D0/en
Priority claimed from GB0305525A external-priority patent/GB0305525D0/en
Priority claimed from GB0306119A external-priority patent/GB0306119D0/en
Priority claimed from GB0307515A external-priority patent/GB0307515D0/en
Priority claimed from GB0310294A external-priority patent/GB0310294D0/en
Priority claimed from GB0313689A external-priority patent/GB0313689D0/en
Priority claimed from GB0320157A external-priority patent/GB0320157D0/en
Priority claimed from GB0322245A external-priority patent/GB0322245D0/en
Priority claimed from GB0325657A external-priority patent/GB0325657D0/en
Priority claimed from GB0329053A external-priority patent/GB0329053D0/en
Application filed by Geneform Technologies Ltd filed Critical Geneform Technologies Ltd
Publication of DE602004021235D1 publication Critical patent/DE602004021235D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Nanotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
DE602004021235T 2003-02-21 2004-02-20 Verfahren, kits und reagenzien zur nukleinsäuresequenzierung Expired - Lifetime DE602004021235D1 (de)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
GB0303964A GB0303964D0 (en) 2003-02-21 2003-02-21 FRET based sequencing technology
GB0305525A GB0305525D0 (en) 2003-03-11 2003-03-11 Inhibitor based sequencing technology
GB0306119A GB0306119D0 (en) 2003-03-18 2003-03-18 Non-incorporable label determined sequencing
GB0307515A GB0307515D0 (en) 2003-04-01 2003-04-01 Non-incorporable label determined sequencing process
GB0310294A GB0310294D0 (en) 2003-05-03 2003-05-03 Non-incorporable label determined sequencing-2
GB0313689A GB0313689D0 (en) 2003-06-13 2003-06-13 Sequencing method-j
GB0320157A GB0320157D0 (en) 2003-08-28 2003-08-28 Sequencing method-M
GB0322245A GB0322245D0 (en) 2003-09-23 2003-09-23 Nucleic acid sequencing method
GB0325657A GB0325657D0 (en) 2003-11-04 2003-11-04 Nucleic acid sequencing method
GB0329053A GB0329053D0 (en) 2003-12-16 2003-12-16 Dye conjugate based sequencing
PCT/GB2004/000709 WO2004074503A2 (en) 2003-02-21 2004-02-20 Nucleic acid sequencing methods, kits and reagents

Publications (1)

Publication Number Publication Date
DE602004021235D1 true DE602004021235D1 (de) 2009-07-09

Family

ID=32913446

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004021235T Expired - Lifetime DE602004021235D1 (de) 2003-02-21 2004-02-20 Verfahren, kits und reagenzien zur nukleinsäuresequenzierung

Country Status (5)

Country Link
US (1) US8399196B2 (de)
EP (1) EP1594987B1 (de)
AT (1) ATE432365T1 (de)
DE (1) DE602004021235D1 (de)
WO (1) WO2004074503A2 (de)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7871777B2 (en) 2005-12-12 2011-01-18 The United States Of America As Represented By The Department Of Health And Human Services Probe for nucleic acid sequencing and methods of use
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US20080145844A1 (en) * 2006-01-25 2008-06-19 Evrogen Joint Stock Company Methods of cDNA preparation
US20070202521A1 (en) * 2006-02-14 2007-08-30 Applera Corporation Single Molecule DNA Sequencing Using Fret Based Dynamic Labeling
CA2622649C (en) * 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
KR101551985B1 (ko) 2007-03-09 2015-09-09 리가가쿠 겐큐쇼 모노뉴클레오시드 또는 모노뉴클레오티드로부터 유도되는 구조를 갖는 화합물, 핵산, 표지 물질, 핵산 검출 방법 및 키트
EP2255014A4 (de) * 2008-02-12 2011-05-18 Pacific Biosciences California Zusammensetzungen und verfahren zur verwendung bei analysereaktionen
US8354233B2 (en) 2008-04-01 2013-01-15 University Of Washington Sequence data by reduction of noise due to carry-over primer
WO2010002939A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Methods for real time single molecule sequencing
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
GB0901593D0 (en) 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
AU2010245304B2 (en) 2009-04-27 2015-06-04 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
US8632975B2 (en) * 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
GB201013153D0 (en) 2010-08-04 2010-09-22 Touchlight Genetics Ltd Primer for production of closed linear DNA
EP2787068A4 (de) * 2011-12-01 2014-10-22 Mitsubishi Rayon Co Basis zur verwendung bei der amplifikation von nucleinsäure sowie verfahren zur amplifikation von nucleinsäure
GB201301178D0 (en) * 2013-01-23 2013-03-06 Dynamic Biosensors Gmbh Method for sequencing a template nucleic acid immobilized on a substrate
WO2014130686A2 (en) * 2013-02-20 2014-08-28 Eve Biomedical, Inc. Methods and compositions for nanostructure-based nucleic acid sequencing
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
EP2971071B1 (de) 2013-03-15 2018-02-28 Illumina, Inc. Mit enzymen verknüpfte nukleotide
US9546398B2 (en) * 2013-11-14 2017-01-17 Agilent Technologies, Inc. Polymerase idling method for single molecule DNA sequencing
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
GB201415789D0 (en) 2014-09-05 2014-10-22 Touchlight Genetics Ltd Synthesis of DNA
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
US11326206B2 (en) 2016-04-07 2022-05-10 Pacific Biosciences Of California, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
CA3021769C (en) 2016-04-29 2021-11-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
CN116064727A (zh) 2016-07-27 2023-05-05 斯坦福大学托管董事会 高度复用荧光成像
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
AU2017313718B2 (en) 2016-08-15 2023-09-14 Pacific Biosciences Of California, Inc. Method and system for sequencing nucleic acids
EP3562962B1 (de) 2016-12-30 2022-01-05 Omniome, Inc. Verfahren und system unter verwendung von unterscheidbaren polymerasen zum nachweis von ternären komplexen und zur identifizierung von verwandten nukleotiden
DK3565905T3 (da) * 2017-01-04 2022-07-25 Mgi Tech Co Ltd Nukleinsyresekvensering ved hjælp af affinitetsreagenser
CA3049722C (en) 2017-01-10 2023-01-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent dna binding
EP3571313B1 (de) 2017-01-20 2020-12-23 Omniome, Inc. Allelspezifische erfassung von nukleinsäuren
US9932631B1 (en) 2017-09-11 2018-04-03 Omniome, Inc. Genotyping by polymerase binding
CA3050695C (en) * 2017-01-20 2024-02-20 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
WO2018232086A1 (en) * 2017-06-14 2018-12-20 Board Of Regents, The University Of Texas System Chip hybridized association-mapping platform and methods of use
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. BACKGROUND NOISE REDUCTION AND STABILIZATION OF SIMULTANEOUS COMPLEXES IN LINKED ASSAY WORKFLOWS
AU2019217824A1 (en) * 2018-02-06 2020-07-30 Omniome, Inc. Compositions and techniques for nucleic acid primer extension
CA3097583A1 (en) * 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
JP2021521820A (ja) 2018-04-26 2021-08-30 オムニオム インコーポレイテッドＯｍｎｉｏｍｅ， Ｉｎｃ． 核酸−ヌクレオチド−ポリメラーゼ複合体を安定化するための方法及び組成物
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
AU2019276719A1 (en) 2018-05-31 2020-11-26 Pacific Biosciences Of California, Inc. Increased signal to noise in nucleic acid sequencing
CA3107165A1 (en) 2018-07-24 2020-01-30 Omniome, Inc. Serial formation of ternary complex species
US10731141B2 (en) 2018-09-17 2020-08-04 Omniome, Inc. Engineered polymerases for improved sequencing
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
EP3890887A4 (de) 2018-12-07 2022-10-12 Element Biosciences, Inc. Durchflusszellenvorrichtung und deren verwendung
US20220177959A1 (en) * 2019-03-07 2022-06-09 The Trustees Of Columbia University In The City Of New York High accuracy nanopore-based single molecule sequencing by synthesis with tagged nucleotides
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US20210139867A1 (en) 2019-11-08 2021-05-13 Omniome, Inc. Engineered polymerases for improved sequencing by binding
US11408032B2 (en) 2020-01-17 2022-08-09 Element Biosciences, Inc. Tube lens design for improved depth-of-field
US11198121B1 (en) * 2020-06-10 2021-12-14 Element Biosciences, Inc. Flow cell systems and devices
WO2023280156A1 (zh) * 2021-07-08 2023-01-12 深圳华大生命科学研究院 修饰的核苷或核苷酸

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358931B1 (en) * 1990-01-11 2002-03-19 Isis Pharmaceuticals, Inc. Compositions and methods for modulating RNA
DE4140463A1 (de) * 1991-12-09 1993-06-17 Boehringer Mannheim Gmbh 2'-desoxy-isoguanosin, dessen isostere analoge sowie anwendung derselben
NZ503765A (en) * 1997-09-12 2002-04-26 Exiqon As Bi-cyclic and tri-cyclic nucleotide analogues
ATE469699T1 (de) 1999-02-23 2010-06-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
CA2368135C (en) * 1999-03-18 2010-06-08 Exiqon A/S Xylo-lna analogues
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU2002361946A1 (en) * 2001-12-18 2003-06-30 Human Genetic Signatures Pty Ltd Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
SE0202867D0 (sv) * 2002-09-27 2002-09-27 Pyrosequencing Ab New sequencing method

Also Published As

Publication number Publication date
US8399196B2 (en) 2013-03-19
ATE432365T1 (de) 2009-06-15
EP1594987B1 (de) 2009-05-27
WO2004074503A3 (en) 2005-03-24
WO2004074503A2 (en) 2004-09-02
US20070148645A1 (en) 2007-06-28
EP1594987A2 (de) 2005-11-16

Similar Documents

Publication Publication Date Title
DE602004021235D1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
WO2004092331A3 (en) Composition and method for nucleic acid sequencing
DE60220025D1 (de) Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
ATE551349T1 (de) Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden
ATE364718T1 (de) Verfahren zur vervielfältigung von nukleinsäure
NO20072722L (no) Preparater og fremgangsmate for a rense nukleinsyrer fra stabiliseringsreagenser
ATE255168T1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
ATE403747T1 (de) Verfahren zur amplifikation von signal- flankierenden sequenzen aus unbekannten, genomischen daten
ATE480644T1 (de) Verfahren zur quantifizierung kleiner rna- moleküle
DE60034396D1 (de) Reaktionsgemisch und Verfahren für RT-PCR mittels einer homopolymeren Nukleinsäure
WO2008033442A3 (en) Methods and compositions for performing low background multiplex nucleic acid amplification reactions
ATE516368T1 (de) Erstellung eines echtzeit-genexpressionsprofils
SE0301951D0 (sv) New method
CN108138175A (zh) 用于分子条形码编码的试剂、试剂盒和方法
WO2007076143A3 (en) Species-specific primer sets and identification of species-specific dna sequences using genome fragment enrichment
DE60326327D1 (de) Verfahren und zusammensetzungen zur identifikation und charakterisierung von hepatitis c
ATE497022T1 (de) Verfahren zur amplifikation von nukleinsäure
ATE466105T1 (de) Verfahren, zusammensetzungen und kits zur anreicherung und zum nachweis von mikroorganismen
ATE420975T1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren
ATE417938T1 (de) Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren
CY1109285T1 (el) Ανακτηση toy dna
DE60324819D1 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus

Legal Events

Date Code Title Description
8364 No opposition during term of opposition