ATE551349T1 - Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden - Google Patents
Methode unter verwendung von reversibel blockierten markierungsoligonukleotidenInfo
- Publication number
- ATE551349T1 ATE551349T1 AT05814417T AT05814417T ATE551349T1 AT E551349 T1 ATE551349 T1 AT E551349T1 AT 05814417 T AT05814417 T AT 05814417T AT 05814417 T AT05814417 T AT 05814417T AT E551349 T1 ATE551349 T1 AT E551349T1
- Authority
- AT
- Austria
- Prior art keywords
- reversibly blocked
- labeling oligonucleotides
- dna molecule
- blocked labeling
- tag
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 238000002372 labelling Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 230000004544 DNA amplification Effects 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CA2005/001830 WO2007062495A1 (en) | 2005-11-30 | 2005-11-30 | Selective terminal tagging of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE551349T1 true ATE551349T1 (de) | 2012-04-15 |
Family
ID=38091822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05814417T ATE551349T1 (de) | 2005-11-30 | 2005-11-30 | Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8304183B2 (de) |
| EP (1) | EP1954706B1 (de) |
| AT (1) | ATE551349T1 (de) |
| AU (1) | AU2005338632B2 (de) |
| CA (1) | CA2639819C (de) |
| WO (1) | WO2007062495A1 (de) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2707436C (en) | 2007-06-29 | 2014-01-28 | Epicentre Technologies Corporation | Copy dna and sense rna |
| DK2644707T3 (en) * | 2008-04-30 | 2015-06-29 | Integrated Dna Tech Inc | RNase-H-based assays using modified RNA monomers. |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP2488664A4 (de) * | 2009-10-13 | 2014-04-09 | Syntezza Molecular Detection Israel Ltd | Verfahren und zusammensetzungen zur verstärkung von zielsequenzen aus nukleinsäureproben |
| WO2011060014A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| EP2534247B1 (de) * | 2010-02-12 | 2015-10-28 | Genisphere, LLC | Vollständige transkriptomsequenzierung |
| CA2807552A1 (en) | 2010-08-06 | 2012-02-09 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| DK3590949T3 (da) | 2010-10-01 | 2022-07-11 | Modernatx Inc | Ribonukleinsyrer indeholdende n1-methyl-pseudouracil og anvendelse heraf |
| AU2012236896A1 (en) * | 2011-03-25 | 2013-05-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| CA2831613A1 (en) | 2011-03-31 | 2012-10-04 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| EP2756098B1 (de) | 2011-09-16 | 2018-06-06 | Lexogen GmbH | Methode zur herstellung einer bibliothek von nukleinsäuremolekülen |
| EP2570487A1 (de) | 2011-09-16 | 2013-03-20 | Lexogen GmbH | Verfahren zur Nukleinsäuretranskription |
| SG10201602654SA (en) | 2011-10-03 | 2016-05-30 | Moderna Therapeutics Inc | Modified nucleosides,nucleotides,and nucleic acids,and uses thereof |
| WO2013090648A1 (en) | 2011-12-16 | 2013-06-20 | modeRNA Therapeutics | Modified nucleoside, nucleotide, and nucleic acid compositions |
| US9303079B2 (en) | 2012-04-02 | 2016-04-05 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| JP2015516143A (ja) | 2012-04-02 | 2015-06-08 | モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | ヒト疾患に関連するタンパク質の産生のための修飾ポリヌクレオチド |
| LT2922554T (lt) | 2012-11-26 | 2022-06-27 | Modernatx, Inc. | Terminaliai modifikuota rnr |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| US9255265B2 (en) | 2013-03-15 | 2016-02-09 | Illumina, Inc. | Methods for producing stranded cDNA libraries |
| EP2971072A1 (de) * | 2013-03-15 | 2016-01-20 | Integrated DNA Technologies Inc. | Tests auf rnase-h-basis unter verwendung modifizierter rna-monomere |
| WO2015048744A2 (en) | 2013-09-30 | 2015-04-02 | Moderna Therapeutics, Inc. | Polynucleotides encoding immune modulating polypeptides |
| AU2014329452B2 (en) | 2013-10-03 | 2019-06-20 | Moderna Therapeutics, Inc. | Polynucleotides encoding low density lipoprotein receptor |
| WO2016081585A1 (en) | 2014-11-20 | 2016-05-26 | Ampliwise Inc. | Compositions and methods for nucleic acid amplification |
| GB2536446B (en) * | 2015-03-17 | 2020-06-03 | Salisbury Nhs Found Trust | PCR method |
| EP3469100B1 (de) | 2016-06-10 | 2021-01-27 | Seegene, Inc. | Verfahren zur herstellung von markierungsoligonukleotiden |
| AU2018254190A1 (en) | 2017-04-17 | 2019-10-31 | Guoliang Fu | Methods, compositions, and kits for preparing nucleic acid libraries |
| FR3067353B1 (fr) | 2017-06-08 | 2020-10-23 | Centre Nat Rech Scient | Copolymere fonctionnel d'un 1,3-diene et d'une olefine |
| FR3067355A1 (fr) | 2017-06-08 | 2018-12-14 | Compagnie Generale Des Etablissements Michelin | Composition de caoutchouc |
| MX2021003847A (es) | 2018-10-04 | 2021-05-27 | Bluestar Genomics Inc | Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual. |
| FR3087443B3 (fr) | 2018-10-23 | 2020-10-23 | Michelin & Cie | Composition de caoutchouc |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
| JP3337748B2 (ja) | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法 |
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| US6383777B1 (en) * | 1998-04-16 | 2002-05-07 | Va{Umlaut Over (N)}Derbilt University | Methods and compositions for high yield production of eukaryotic proteins |
| EP1100972B1 (de) * | 1998-07-31 | 2006-06-14 | Gen-Probe Incorporated | Reversibel inhibierende sonden |
| US6132997A (en) * | 1999-05-28 | 2000-10-17 | Agilent Technologies | Method for linear mRNA amplification |
| GB0118758D0 (en) | 2001-08-01 | 2001-09-26 | Sybesma Wilbert F H | Chimeric primed based real time rt-pcr for qualification of rna or dna templates in a crude sample |
| US20050153333A1 (en) | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| WO2005098044A1 (en) * | 2004-04-01 | 2005-10-20 | Genisphere Inc. | Methods for amplification of nucleic acid sequences using promoter templates |
-
2005
- 2005-11-30 WO PCT/CA2005/001830 patent/WO2007062495A1/en active Application Filing
- 2005-11-30 AU AU2005338632A patent/AU2005338632B2/en active Active
- 2005-11-30 AT AT05814417T patent/ATE551349T1/de active
- 2005-11-30 CA CA2639819A patent/CA2639819C/en active Active
- 2005-11-30 EP EP05814417A patent/EP1954706B1/de not_active Not-in-force
- 2005-11-30 US US12/095,409 patent/US8304183B2/en active Active
-
2012
- 2012-09-26 US US13/627,616 patent/US20130029881A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1954706A1 (de) | 2008-08-13 |
| AU2005338632A1 (en) | 2007-06-07 |
| EP1954706B1 (de) | 2012-03-28 |
| CA2639819C (en) | 2012-10-23 |
| WO2007062495A1 (en) | 2007-06-07 |
| US8304183B2 (en) | 2012-11-06 |
| CA2639819A1 (en) | 2007-06-07 |
| AU2005338632B2 (en) | 2010-05-20 |
| EP1954706A4 (de) | 2009-11-18 |
| US20130029881A1 (en) | 2013-01-31 |
| US20090227009A1 (en) | 2009-09-10 |
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