CY1119878T1 - Μια μεθοδος για απομονωση πυρηνικων οξεων και ενα kit εξ' αυτης - Google Patents

Μια μεθοδος για απομονωση πυρηνικων οξεων και ενα kit εξ' αυτης

Info

Publication number
CY1119878T1
CY1119878T1 CY20171101226T CY171101226T CY1119878T1 CY 1119878 T1 CY1119878 T1 CY 1119878T1 CY 20171101226 T CY20171101226 T CY 20171101226T CY 171101226 T CY171101226 T CY 171101226T CY 1119878 T1 CY1119878 T1 CY 1119878T1
Authority
CY
Cyprus
Prior art keywords
nucleic acids
cotton
dna
bound
acid
Prior art date
Application number
CY20171101226T
Other languages
English (en)
Inventor
Phani Kumar Pullela
Mulakkapurath Narayanan Manoj
Santhosh Kumar Gandhavalla
Mitchell Preetham Pinto
Chandrasekhar Bhaskaran Nair
Pillarisetti Venkata Subbarao
Original Assignee
Bigtec Private Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bigtec Private Limited filed Critical Bigtec Private Limited
Publication of CY1119878T1 publication Critical patent/CY1119878T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Η παρούσα αποκάλυψη παρέχει μια μέθοδο για απομόνωση φυσικών & τεχνητών πυρηνικών οξέων όπως τα δεοξυριβονουκλεϊκό οξύ (DNA), ριβονουκλεϊκό οξύ (RNA) και πυρηνικό οξύ πεπτιδίων (ΡΝΑ) από ένα στερεό ή υγρό δείγμα χρησιμοποιώντας βάμβακα. Ο βάμβαξ που συσκευάζεται είναι έτσι ώστε, ένα διάλυμα που περιέχει πυρηνικά οξέα να διέρχεται μέσα από αυτόν και τα πυρηνικά οξέα σε διάλυμα να δεσμεύονται στον βάμβακα σε ένα βέλτιστο μέσο για δέσμευση. Τα πυρηνικά οξέα δεσμεύονται σε βάμβακα κατά τέτοιο τρόπο ώστε, τα δεσμευθέντα πυρηνικά οξέα να μπορούν να αντέξουν πολλαπλές πλύσεις με υγρό περιλαμβάνοντας ύδωρ και που να εκλούονται σε ένα υδατικό ρυθμιστικό, με το οποίο τα εκλουσθέντα πυρηνικά οξέα να μπορούν να χρησιμοποιηθούν άμεσα για ενίσχυση χρησιμοποιώντας PCR ή για τις οποιεσδήποτε άλλες βιοχημικές ανάγκες ή ανάγκες μοριακής βιολογίας.
CY20171101226T 2010-01-07 2017-11-23 Μια μεθοδος για απομονωση πυρηνικων οξεων και ενα kit εξ' αυτης CY1119878T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN50CH2010 2010-01-07
PCT/IB2011/050044 WO2011083429A1 (en) 2010-01-07 2011-01-06 A method for isolation of nucleic acids and a kit thereof

Publications (1)

Publication Number Publication Date
CY1119878T1 true CY1119878T1 (el) 2018-06-27

Family

ID=44305232

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20171101226T CY1119878T1 (el) 2010-01-07 2017-11-23 Μια μεθοδος για απομονωση πυρηνικων οξεων και ενα kit εξ' αυτης

Country Status (21)

Country Link
US (1) US8986976B2 (el)
EP (1) EP2521780B1 (el)
JP (1) JP5791631B2 (el)
KR (1) KR101590544B1 (el)
CN (1) CN102725407B (el)
BR (1) BR112012016774B1 (el)
CY (1) CY1119878T1 (el)
DK (1) DK2521780T3 (el)
EA (1) EA022842B1 (el)
ES (1) ES2652335T3 (el)
HR (1) HRP20171823T1 (el)
HU (1) HUE034870T2 (el)
LT (1) LT2521780T (el)
NO (1) NO2521780T3 (el)
PL (1) PL2521780T3 (el)
PT (1) PT2521780T (el)
RS (1) RS56636B1 (el)
SG (1) SG182372A1 (el)
SI (1) SI2521780T1 (el)
WO (1) WO2011083429A1 (el)
ZA (1) ZA201205053B (el)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201020095D0 (en) 2010-11-26 2011-01-12 Invitrogen Dynal As Nucleic acid preparation method
CN102286462A (zh) * 2011-06-27 2011-12-21 深圳市易瑞生物技术有限公司 一种游离rna提取方法及试剂盒
DE102011080853B4 (de) * 2011-08-11 2014-03-27 Axagarius Gmbh & Co. Kg Verfahren zur Isolierung von RNA aus Volblutproben
JP2015514216A (ja) * 2012-03-27 2015-05-18 ノースウエスタン ユニバーシティ 試料採集及び調製のための容器及びシステム
US9540635B2 (en) * 2012-05-09 2017-01-10 Bio-Rad Laboratories, Inc. Buffer for one-step DNA extraction
CN103571825B (zh) * 2012-08-09 2016-03-16 财团法人工业技术研究院 用于生物样本处理之组合物及使用其之核酸扩增方法
EP2898069B1 (en) * 2012-09-19 2018-04-11 Beckman Coulter, Inc. Use of divalent ions, proteases, detergents, and low ph in the extraction of nucleic acids
EP2900819B1 (en) 2012-09-28 2020-10-28 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
CN103882006A (zh) * 2012-12-19 2014-06-25 河北省健海生物芯片技术有限责任公司 适用于多种样本的dna提取试剂配制方法和提取方法
CN103289990A (zh) * 2013-06-14 2013-09-11 浙江世纪康大医疗科技有限公司 一种血液dna的快速提取方法
WO2015022410A1 (en) * 2013-08-16 2015-02-19 General Electric Company Methods and compositions for extraction and storage of nucleic acids
TWI642679B (zh) * 2014-07-29 2018-12-01 精專生醫股份有限公司 萃取核酸分子之裝置及其使用方法
CN105734044A (zh) * 2014-12-08 2016-07-06 苏州新波生物技术有限公司 用于核酸提取纯化的漂洗液
CN104911176A (zh) * 2015-01-25 2015-09-16 江苏发士达生物科技有限公司 一种细菌dna提取液、提取方法及其应用
GB201504459D0 (en) 2015-03-17 2015-04-29 Moorlodge Biotech Ventures Ltd Isolation of DNA
JP7099951B2 (ja) * 2015-07-24 2022-07-12 セファイド 組織試料からのｄｎａ及びｒｎａ抽出のための組成物及び方法
CN105624149A (zh) * 2016-01-16 2016-06-01 长春市博坤生物科技有限公司 一种用于高难接触性检材dna提取纯化的试剂盒及方法
CN105420231A (zh) * 2016-01-21 2016-03-23 杭州和壹基因科技有限公司 一种从人体唾液中提取基因组dna的方法
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
BR112018017304A2 (pt) * 2016-02-23 2019-01-02 Bigtec Private Ltd cartucho para purificar uma amostra e análise
RU2628695C1 (ru) * 2016-06-14 2017-08-21 Общество с ограниченной ответственностью "Биологическая среда" Способ выделения РНК и ДНК из сухих биологических образцов, хранившихся на бумажном носителе, и набор для его осуществления
GB2551801A (en) * 2016-06-30 2018-01-03 Lgc Genomics Ltd Methods
RU2650865C1 (ru) * 2016-11-29 2018-04-17 Общество с ограниченной ответственностью "Магнэтик" Набор реактивов для выделения днк
CN106801068B (zh) * 2017-01-12 2020-03-17 西安交通大学 一种自发荧光可降解聚柠檬酸酯的非病毒基因载体及其制备方法
RU2651937C1 (ru) * 2017-05-04 2018-04-24 Татьяна Георгиевна Фалеева Композиция для сбора и хранения днк или днк-содержащих биологических следов (варианты) и её применение
CN109207472B (zh) * 2017-07-06 2023-10-20 上海科华生物工程股份有限公司 Dna病毒核酸提取试剂盒及其使用方法
CN109385418B (zh) * 2017-08-03 2022-07-22 杭州优思达生物技术有限公司 一种用于动物样本中病毒/细菌核酸提取的方法及试剂
WO2019067660A1 (en) * 2017-09-27 2019-04-04 University of North Carolina Wilmington TOOL FOR DETECTION OF PATHOGENIC AGENTS DERIVED FROM MAN AND HUMAN WASTEWATER
CN107723285A (zh) * 2017-10-18 2018-02-23 中山大学肿瘤防治中心 一种通用于常规生物样本的总dna提取方法及提取试剂盒
US20200277650A1 (en) 2017-11-16 2020-09-03 Matjaz Vogelsang Polynucleotide-binding protein for use in diagnosis
CN107904232B (zh) * 2017-12-29 2020-10-16 浙江今复康生物科技有限公司 一种从痰液中快速提取核酸的方法
CN109932359A (zh) * 2019-02-11 2019-06-25 张丽英 一种利用atp生物发光反应检测食品中肉毒杆菌的方法
CN109897851A (zh) * 2019-04-12 2019-06-18 武汉科技大学 小分子dna纯化试剂
CN111826420B (zh) * 2019-04-15 2023-06-23 南京林业大学 松材线虫核酸提取试剂及其应用
CN110029105A (zh) * 2019-05-14 2019-07-19 成都罗宁生物科技有限公司 一种提取微生物dna的试剂盒及其方法
CN113025608A (zh) * 2019-12-09 2021-06-25 深圳市真迈生物科技有限公司 细胞裂解液、试剂盒及应用
CN110938624A (zh) * 2019-12-27 2020-03-31 深圳市海普洛斯生物科技有限公司 一种用于基因组dna提取的试剂盒及其应用
CN111334503A (zh) * 2020-04-07 2020-06-26 江苏康为世纪生物科技有限公司 一种核酸保存液
CN112176033A (zh) * 2020-11-12 2021-01-05 苏州创澜生物科技有限公司 一种用于rna提取的裂解缓冲液
CN112239775A (zh) * 2020-11-12 2021-01-19 苏州创澜生物科技有限公司 一种用于核酸提取的洗涤缓冲液
CN112725413B (zh) * 2021-02-05 2022-10-14 厦门甄准生物技术有限公司 一种宫颈拭子核酸提取的裂解结合液、试剂盒及方法
KR102438028B1 (ko) * 2021-07-15 2022-08-30 주식회사 지엠디바이오텍 휴대용 성분 추출 스포이트 및 성분 추출 방법
WO2024025921A1 (en) * 2022-07-26 2024-02-01 Siemens Healthcare Diagnostics Inc. Extraction method for nucleic acids
WO2024080275A1 (ja) * 2022-10-10 2024-04-18 国立大学法人山口大学 環境ｄｎａ又は環境ｒｎａの回収方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19530132C2 (de) 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5804684A (en) 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US7217513B2 (en) 2001-07-10 2007-05-15 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
EP2258845B1 (en) 2001-11-06 2017-08-23 Promega Corporation Isolation and purification of nucleic acids
JP2004201607A (ja) * 2002-12-26 2004-07-22 Asahi Kasei Corp 核酸吸着固相体上でのｌａｍｐ反応
AU2004220626B2 (en) * 2003-02-05 2010-07-29 Iquum Inc. Sample processing tubule
JP4831725B2 (ja) * 2004-02-13 2011-12-07 栄研化学株式会社 簡易的核酸抽出法
CN101048515B (zh) * 2004-08-31 2013-06-12 荣研化学株式会社 核酸分析方法
WO2008150187A1 (fr) * 2007-06-08 2008-12-11 Jury Fedorovich Drygin Utilisation du trichloracétate d'ammonium en tant que produit de dissociation de complexes naturels d'acides nucléiques et procédé d'extraction d'arn

Also Published As

Publication number Publication date
KR20120104625A (ko) 2012-09-21
BR112012016774A2 (pt) 2019-09-10
CN102725407B (zh) 2016-09-14
NO2521780T3 (el) 2018-01-20
HRP20171823T1 (hr) 2018-01-12
EP2521780A1 (en) 2012-11-14
ZA201205053B (en) 2013-03-27
CN102725407A (zh) 2012-10-10
DK2521780T3 (en) 2017-12-04
EP2521780A4 (en) 2013-07-31
RS56636B1 (sr) 2018-03-30
SG182372A1 (en) 2012-08-30
JP2013516186A (ja) 2013-05-13
US8986976B2 (en) 2015-03-24
ES2652335T3 (es) 2018-02-01
PT2521780T (pt) 2017-12-21
BR112012016774B1 (pt) 2020-12-01
LT2521780T (lt) 2018-02-12
HUE034870T2 (hu) 2018-03-28
EA201290612A1 (ru) 2013-02-28
EP2521780B1 (en) 2017-08-23
WO2011083429A1 (en) 2011-07-14
EA022842B1 (ru) 2016-03-31
PL2521780T3 (pl) 2018-03-30
KR101590544B1 (ko) 2016-02-18
JP5791631B2 (ja) 2015-10-07
SI2521780T1 (en) 2018-02-28
US20130203150A1 (en) 2013-08-08

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