AR048989A1 - Metodos y composiciones para modular la expresion en plantas - Google Patents

Metodos y composiciones para modular la expresion en plantas

Info

Publication number
AR048989A1
AR048989A1 ARP010100266A ARP010100266A AR048989A1 AR 048989 A1 AR048989 A1 AR 048989A1 AR P010100266 A ARP010100266 A AR P010100266A AR P010100266 A ARP010100266 A AR P010100266A AR 048989 A1 AR048989 A1 AR 048989A1
Authority
AR
Argentina
Prior art keywords
zinc finger
finger protein
expression
plant
target gene
Prior art date
Application number
ARP010100266A
Other languages
English (en)
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of AR048989A1 publication Critical patent/AR048989A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

El uso de proteínas de dedo de zinc y las fusiones de dichas proteínas para regular la expresion de los genes y las vías metabolicas en las plantas. Su uso en el campo de la tecnología de la agricultura de las plantas. Un anticuerpo que se une a la proteína de dedo de zinc así como fragmentos de ácido nucleico que codifican tales proteínas. Reivindicacion 1: Un método para modular la expresion de un gen meta en las células de las plantas, cuyo método comprende suministrar una proteína de dedo de zinc a las células de las plantas, siendo dicha proteína de dedo de zinc capaz de unirse específicamente, a una secuencia de nucléotidos meta o a una hebra complementaria de la misma, dentro de un gen meta y que permite a dicha proteína de dedo de zinc unirse a dicha secuencia de nucléotidos meta, por lo cual la expresion de dicho gen meta es modulada en dichas células de plantas. Reivindicacion 74: Un vector de expresion para modular la expresion en las células de las plantas, dicho vector de expresion comprende una secuencia de nucléotidos que codifica una proteína de dedo de zinc, dicha proteína de dedo de zinc es capaz de unirse o de unirse de manera específica a una secuencia de nucléotidos meta o a una hebra complementaria de la misma, dentro de un gen meta cuya expresion va a ser modulada por dicha proteína de dedo de zinc en las células de plantas. Reivindicacion 76: Una célula de planta modificada genéticamente, dicha célula comprende un sistema de expresion para una proteína de dedo de zinc, dicha proteína de dedo de zinc es capaz de unirse, y preferentemente, a las células de las plantas, de unirse de manera específica, a una secuencia de nucléotidos meta, o a una hebra complementaria de la misma, dentro de un gen meta, cuya expresion va a ser modulada por dicha proteína de dedo de zinc. Reivindicacion 98: Una planta que se regenera a partir de una planta transformada con el vector de expresion de la reivindicacion 74. Reivindicacion 103: Una proteína de dedo de zinc que se selecciona a partir del grupo que consiste en ZFPm1, ZFPm2, ZFPm3, ZFPm4 y ZFPAp3.
ARP010100266A 2000-01-21 2001-01-22 Metodos y composiciones para modular la expresion en plantas AR048989A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17746800P 2000-01-21 2000-01-21
US62089700A 2000-07-21 2000-07-21

Publications (1)

Publication Number Publication Date
AR048989A1 true AR048989A1 (es) 2006-06-21

Family

ID=26873328

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP010100266A AR048989A1 (es) 2000-01-21 2001-01-22 Metodos y composiciones para modular la expresion en plantas

Country Status (5)

Country Link
EP (1) EP1276869A2 (es)
AR (1) AR048989A1 (es)
AU (1) AU2964101A (es)
CA (1) CA2396898A1 (es)
WO (1) WO2001052620A2 (es)

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Also Published As

Publication number Publication date
CA2396898A1 (en) 2001-07-26
EP1276869A2 (en) 2003-01-22
WO2001052620A2 (en) 2001-07-26
WO2001052620A3 (en) 2002-02-07
AU2964101A (en) 2001-07-31

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