ATE459728T1 - Verfahren zur amplifikation polymerer nukleinsäuren - Google Patents

Verfahren zur amplifikation polymerer nukleinsäuren

Info

Publication number
ATE459728T1
ATE459728T1 AT04779936T AT04779936T ATE459728T1 AT E459728 T1 ATE459728 T1 AT E459728T1 AT 04779936 T AT04779936 T AT 04779936T AT 04779936 T AT04779936 T AT 04779936T AT E459728 T1 ATE459728 T1 AT E459728T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid polymer
primer
primers
hybridizes
Prior art date
Application number
AT04779936T
Other languages
English (en)
Inventor
Mark Behlke
Joseph Walder
Jeffrey Manthey
Original Assignee
Integrated Dna Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Dna Tech Inc filed Critical Integrated Dna Tech Inc
Application granted granted Critical
Publication of ATE459728T1 publication Critical patent/ATE459728T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
  • Saccharide Compounds (AREA)
AT04779936T 2003-08-01 2004-08-02 Verfahren zur amplifikation polymerer nukleinsäuren ATE459728T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49212003P 2003-08-01 2003-08-01
PCT/US2004/025016 WO2005012499A2 (en) 2003-08-01 2004-08-02 Methods for amplifying polymeric nucleic acids

Publications (1)

Publication Number Publication Date
ATE459728T1 true ATE459728T1 (de) 2010-03-15

Family

ID=34115599

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04779936T ATE459728T1 (de) 2003-08-01 2004-08-02 Verfahren zur amplifikation polymerer nukleinsäuren

Country Status (7)

Country Link
EP (1) EP1649065B1 (de)
JP (1) JP2007501005A (de)
AT (1) ATE459728T1 (de)
AU (1) AU2004262037A1 (de)
CA (1) CA2533305A1 (de)
DE (1) DE602004025815D1 (de)
WO (1) WO2005012499A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008043987A2 (en) * 2006-10-09 2008-04-17 Oxitec Limited Methods for amplifying and detecting nucleic acid sequences
EP2324124B1 (de) * 2008-08-12 2012-04-25 Roche Diagnostics GmbH Korrekturlese-primerverlängerung
JP2013511285A (ja) 2009-11-23 2013-04-04 スイフト・バイオサイエンシズ・インコーポレイテツド 一本鎖標的分子を伸長させるデバイス
JP6556728B2 (ja) * 2013-09-13 2019-08-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ポリＡ−キャリヤーにより誘導される高分子量ＰＣＲ生成物の生成を避けるためのオリゴ−ｄＴ分子の適用
GB2527115A (en) * 2014-06-12 2015-12-16 Vela Operations Pte Ltd Improved NGS workflow

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6335184B1 (en) 1993-07-23 2002-01-01 Bio-Rad Laboratories, Inc. Linked linear amplification of nucleic acids
US6093807A (en) * 1999-03-19 2000-07-25 Isis Pharmaceuticals, Inc. Sugar-modified 7-deaza-7-substituted oligonucleotides
US7205129B1 (en) * 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
CA2476564C (en) * 2002-03-01 2012-05-15 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
US20030228596A1 (en) * 2002-03-14 2003-12-11 Qiang Liu Template-driven nucleic acid amplifications

Also Published As

Publication number Publication date
DE602004025815D1 (de) 2010-04-15
AU2004262037A1 (en) 2005-02-10
EP1649065A4 (de) 2007-10-10
JP2007501005A (ja) 2007-01-25
CA2533305A1 (en) 2005-02-10
WO2005012499A2 (en) 2005-02-10
EP1649065B1 (de) 2010-03-03
WO2005012499A3 (en) 2005-09-15
EP1649065A2 (de) 2006-04-26

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Legal Events

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