CN102533960B - 一种单细胞基因组分析方法及试剂盒 - Google Patents
一种单细胞基因组分析方法及试剂盒 Download PDFInfo
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- CN102533960B CN102533960B CN201010619689.8A CN201010619689A CN102533960B CN 102533960 B CN102533960 B CN 102533960B CN 201010619689 A CN201010619689 A CN 201010619689A CN 102533960 B CN102533960 B CN 102533960B
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Abstract
Description
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Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201010619689.8A CN102533960B (zh) | 2010-12-31 | 2010-12-31 | 一种单细胞基因组分析方法及试剂盒 |
US13/976,845 US9238840B2 (en) | 2010-12-31 | 2011-12-29 | Method for single cell genome analysis and kit therefor |
EP11853098.9A EP2660331B1 (en) | 2010-12-31 | 2011-12-29 | Method for single cell genome analysis and kit therefor |
PCT/CN2011/084959 WO2012089148A1 (zh) | 2010-12-31 | 2011-12-29 | 单细胞基因组分析方法及试剂盒 |
HK12110109.3A HK1169454A1 (zh) | 2010-12-31 | 2012-10-12 | 種單細胞基因組分析方法及試劑盒 |
Applications Claiming Priority (1)
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CN201010619689.8A CN102533960B (zh) | 2010-12-31 | 2010-12-31 | 一种单细胞基因组分析方法及试剂盒 |
Publications (2)
Publication Number | Publication Date |
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CN102533960A CN102533960A (zh) | 2012-07-04 |
CN102533960B true CN102533960B (zh) | 2014-04-30 |
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CN201010619689.8A Active CN102533960B (zh) | 2010-12-31 | 2010-12-31 | 一种单细胞基因组分析方法及试剂盒 |
Country Status (5)
Country | Link |
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US (1) | US9238840B2 (zh) |
EP (1) | EP2660331B1 (zh) |
CN (1) | CN102533960B (zh) |
HK (1) | HK1169454A1 (zh) |
WO (1) | WO2012089148A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112359097A (zh) * | 2014-11-28 | 2021-02-12 | 深圳市海普洛斯生物科技有限公司 | 单细胞的全基因组的扩增方法及试剂盒 |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20150299753A1 (en) * | 2012-03-30 | 2015-10-22 | Bgi Tech Solutions Co., Ltd. | Whole genome amplification method and application thereof |
CN104797718B (zh) * | 2012-09-12 | 2020-05-08 | 加利福尼亚大学董事会 | 通过单链扩增和测序对单个细胞进行精确基因组测序 |
PT2881739T (pt) * | 2013-12-04 | 2017-08-11 | Fraunhofer Ges Forschung | Método e estojo para determinação da integridade do genoma e/ou da qualidade de uma biblioteca de sequências de adn obtida através de amplificação determinística do genoma inteiro por locais de restrição |
US10227650B2 (en) | 2014-11-14 | 2019-03-12 | Athena Diagnostics, Inc. | Methods to detect a silent carrier of a null allele genotype |
CN104404034A (zh) * | 2014-12-15 | 2015-03-11 | 赛业健康研究中心(太仓)有限公司 | 利用口腔上皮细胞制作单细胞测序参考品的方法 |
CN104450923A (zh) * | 2014-12-15 | 2015-03-25 | 赛业健康研究中心(太仓)有限公司 | 利用囊胚腔液游离dna检测胚胎染色体异常的方法 |
CN105861486A (zh) * | 2015-01-19 | 2016-08-17 | 深圳华大基因科技服务有限公司 | 从混合核酸中获得目标核酸的方法 |
WO2016123692A1 (en) * | 2015-02-04 | 2016-08-11 | The University Of British Columbia | Methods and devices for analyzing particles |
EP3436605A4 (en) * | 2016-03-31 | 2019-08-21 | Perkinelmer Health Sciences (Australia) Pty Ltd | AMPLIFICATION OF TARGET SEQUENCES |
CN106520997A (zh) * | 2016-12-14 | 2017-03-22 | 南京诺唯赞医疗科技有限公司 | 一种单细胞基因表达定量分析的方法 |
CN110787296B (zh) * | 2018-08-01 | 2024-04-16 | 复旦大学附属肿瘤医院 | 一种用于预防或治疗胰腺癌的药物组合物及检测胰腺癌的试剂盒 |
US10988815B2 (en) * | 2018-12-17 | 2021-04-27 | National Cheng Kung University | Detective molecule, kit and method for predicting fragrance production in an orchid |
CN111379032A (zh) * | 2018-12-28 | 2020-07-07 | 北京贝瑞和康生物技术有限公司 | 一种用于构建同时实现基因组拷贝数变异检测和基因突变检测的测序文库的方法和试剂盒 |
CN112662748B (zh) * | 2019-10-15 | 2023-02-17 | 骏实生物科技(上海)有限公司 | 用于单细胞dna测序文库质量鉴定的引物对组合、方法和试剂盒 |
CN113584138A (zh) * | 2021-09-07 | 2021-11-02 | 广西壮族自治区妇幼保健院 | 一种对mda单细胞全基因组扩增产物的质控方法 |
CN113684266A (zh) * | 2021-09-10 | 2021-11-23 | 苏州贝康医疗器械有限公司 | 用于单细胞全基因组dna扩增产物质量评价的核酸组合物及方法 |
CN117233393B (zh) * | 2023-11-15 | 2024-02-09 | 四川大学华西医院 | 双重免疫组化染色试剂盒及其在鉴别良恶性胆管上皮肿瘤中的应用 |
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US6673541B1 (en) * | 1998-09-18 | 2004-01-06 | Micromet Ag | DNA amplification of a single cell |
US7297485B2 (en) * | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
US20040229233A1 (en) * | 2002-10-16 | 2004-11-18 | Ngk Insulators, Ltd. | Human housekeeping genes and human-tissue specific genes |
AU2003299694A1 (en) * | 2002-12-20 | 2004-07-22 | Qiagen Gmbh | Nucleic acid amplification |
DE602004028478D1 (de) * | 2003-06-17 | 2010-09-16 | Human Genetic Signatures Pty | Verfahren zur genomamplifikation |
US7387876B2 (en) * | 2004-02-27 | 2008-06-17 | President And Fellows Of Harvard College | Amplification of trace amounts of nucleic acids |
JP3972106B2 (ja) * | 2004-03-03 | 2007-09-05 | 大学共同利用機関法人情報・システム研究機構 | ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー |
US20060172314A1 (en) * | 2005-01-31 | 2006-08-03 | Song Min-Sun | Quantification of amplified nucleic acids |
TWI367260B (en) | 2005-03-18 | 2012-07-01 | Univ Hong Kong Chinese | Method for detecting the presence of a fetus with trisomy 21 in a pregnant woman and kit thereof |
CN101333561A (zh) * | 2007-06-29 | 2008-12-31 | 上海裕隆生物科技有限公司 | 心脑血管疾病易感基因芯片检测试剂盒 |
ATE509123T1 (de) * | 2007-10-16 | 2011-05-15 | Hoffmann La Roche | Hochauflösende hochdurchsatz-hla-genotypisierung mittels klonaler sequenzierung |
US20110294167A1 (en) * | 2008-12-02 | 2011-12-01 | Kapabiosystems | Nucleic acid amplification |
CN102333891A (zh) * | 2009-01-20 | 2012-01-25 | 利兰·斯坦福青年大学托管委员会 | 用于药靶诊断、预后和鉴别的单细胞基因表达 |
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2010
- 2010-12-31 CN CN201010619689.8A patent/CN102533960B/zh active Active
-
2011
- 2011-12-29 US US13/976,845 patent/US9238840B2/en active Active
- 2011-12-29 EP EP11853098.9A patent/EP2660331B1/en active Active
- 2011-12-29 WO PCT/CN2011/084959 patent/WO2012089148A1/zh active Application Filing
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2012
- 2012-10-12 HK HK12110109.3A patent/HK1169454A1/zh unknown
Non-Patent Citations (8)
Title |
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Balanced‐PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples;Gang Wang et al.;《Nucleic Acids Research》;20040521;第32卷(第9期);第4页左栏第2段,第3页表1,第2页左栏第2段 * |
Gang Wang et al..Balanced‐PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples.《Nucleic Acids Research》.2004,第32卷(第9期),e76. |
Roger S Lasken.Single-cell genomic sequencing using Multiple Displacement Amplification.《Antimicrobials/Genomics》.2007,第10卷(第5期),510-516. |
Sébastien Rodrigue et al..Whole Genome Amplification and De novo Assembly of Single Bacterial Cells.《PLOS one》.2009,第4卷(第9期),e6864. |
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Single-cell genomic sequencing using Multiple Displacement Amplification;Roger S Lasken;《Antimicrobials/Genomics》;20071031;第10卷(第5期);510-516 * |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112359097A (zh) * | 2014-11-28 | 2021-02-12 | 深圳市海普洛斯生物科技有限公司 | 单细胞的全基因组的扩增方法及试剂盒 |
Also Published As
Publication number | Publication date |
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CN102533960A (zh) | 2012-07-04 |
WO2012089148A1 (zh) | 2012-07-05 |
EP2660331A4 (en) | 2015-04-15 |
EP2660331B1 (en) | 2017-10-04 |
EP2660331A1 (en) | 2013-11-06 |
HK1169454A1 (zh) | 2013-01-25 |
US20140017683A1 (en) | 2014-01-16 |
US9238840B2 (en) | 2016-01-19 |
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