DK1604044T3 - Genvinding af DNA - Google Patents

Genvinding af DNA

Info

Publication number
DK1604044T3
DK1604044T3 DK04721937T DK04721937T DK1604044T3 DK 1604044 T3 DK1604044 T3 DK 1604044T3 DK 04721937 T DK04721937 T DK 04721937T DK 04721937 T DK04721937 T DK 04721937T DK 1604044 T3 DK1604044 T3 DK 1604044T3
Authority
DK
Denmark
Prior art keywords
mrna
cdna
methods
recovery
region
Prior art date
Application number
DK04721937T
Other languages
English (en)
Inventor
Mingyue He
Alison M Jackson
Original Assignee
Crescendo Biolog Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0306305A external-priority patent/GB0306305D0/en
Priority claimed from GB0311351A external-priority patent/GB0311351D0/en
Application filed by Crescendo Biolog Ltd filed Critical Crescendo Biolog Ltd
Application granted granted Critical
Publication of DK1604044T3 publication Critical patent/DK1604044T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1041Ribosome/Polysome display, e.g. SPERT, ARM

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Control Of Throttle Valves Provided In The Intake System Or In The Exhaust System (AREA)
  • Valve Device For Special Equipments (AREA)
DK04721937T 2003-03-19 2004-03-19 Genvinding af DNA DK1604044T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0306305A GB0306305D0 (en) 2003-03-19 2003-03-19 Recovery of DNA
GB0311351A GB0311351D0 (en) 2003-05-16 2003-05-16 Activity-directed in vitro protein cloning and identification
PCT/GB2004/001211 WO2004083461A1 (en) 2003-03-19 2004-03-19 Recovery of dna

Publications (1)

Publication Number Publication Date
DK1604044T3 true DK1604044T3 (da) 2009-08-31

Family

ID=33031411

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04721937T DK1604044T3 (da) 2003-03-19 2004-03-19 Genvinding af DNA

Country Status (9)

Country Link
US (1) US7572577B2 (da)
EP (1) EP1604044B1 (da)
AT (1) ATE431430T1 (da)
CY (1) CY1109285T1 (da)
DE (1) DE602004021094D1 (da)
DK (1) DK1604044T3 (da)
ES (1) ES2326649T3 (da)
PT (1) PT1604044E (da)
WO (2) WO2004083428A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3629022A1 (en) 2008-07-25 2020-04-01 Richard W. Wagner Protein screening methods
CN109476695A (zh) * 2016-06-27 2019-03-15 丹娜法伯癌症研究院 用于测定rna翻译速率的方法
CN110904135B (zh) * 2019-12-30 2021-08-13 苏州珀罗汀生物技术有限公司 蛋白质基础表达体系、合成系统及制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
ATE161586T1 (de) * 1991-10-11 1998-01-15 Behringwerke Ag Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung
DK0985032T4 (da) * 1997-05-28 2009-08-31 Crescendo Biolog Ltd Ribosom-komplekser som selektionspartikler til in vitro fremvisning og evolution af proteiner
US6207424B1 (en) 1999-11-23 2001-03-27 Maxim Biotech, Inc. Self-primed amplification system
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA

Also Published As

Publication number Publication date
DE602004021094D1 (de) 2009-06-25
CY1109285T1 (el) 2014-07-02
ES2326649T3 (es) 2009-10-16
WO2004083461A1 (en) 2004-09-30
EP1604044B1 (en) 2009-05-13
EP1604044A1 (en) 2005-12-14
PT1604044E (pt) 2009-08-05
US20060281086A1 (en) 2006-12-14
US7572577B2 (en) 2009-08-11
WO2004083461A9 (en) 2004-12-29
ATE431430T1 (de) 2009-05-15
WO2004083428A1 (en) 2004-09-30

Similar Documents

Publication Publication Date Title
WO2004092418A3 (en) Global amplification using a randomly primed composite primer
WO2003062389A3 (en) UNIVERSAL RT-COUPLED PCR METHOD FOR THE SPECIFIC AMPLIFICATION OF mRNA
DE112006002611A5 (de) Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
DK1218542T3 (da) Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
WO2007103018A3 (en) Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof
WO2002044399A3 (en) In vitro transcription method for rna amplification
JP5200302B2 (ja) ヘリカーゼを使用するrnaターゲットの同定
DE50311731D1 (de) Vermehrung von ribonukleinsäuren
ATE476523T1 (de) Verfahren zur genomamplifikation
DK1654388T3 (da) Fremgangsmåde til påvisning af cytosinmethyleringer i DNA
DK2210954T3 (da) Bestemmelse af DNA-methyleringsniveau
WO2004068112A3 (en) Hairpin primer amplification
ATE455868T1 (de) Polymerase-kettenreaktionsverfahren unter einsatz einer dna-polymerase mit proofreading- eigenschaften
CY1109285T1 (el) Ανακτηση toy dna
DE60331072D1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
MX2021014430A (es) Método para amplificar arnms y para preparar bibliotecas de arnm de longitud total.
DE502006008330D1 (de) Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
WO2008045431A3 (en) Rapid reverse transcription of rna
WO2005070027A3 (en) A novel nucleotide mixture for improved nucleic acid amplification performance
DE50312117D1 (de) Verfahren zur Amplifikation genetischer Information mittels an mehrere Stellen im Genom bindende Primer
WO2006138716A3 (en) Thermodynamically defined gene specific primers
ATE470710T1 (de) Auf nasba basiertes detektionsverfahren zur detektion des tuberkelbazillus
GB0216026D0 (en) Nucleic acid amplification method
PL432230A1 (pl) Sposób amplifikacji komplementarnego DNA w reakcji łańcuchowej polimerazy w czasie rzeczywistym z odwrotną transkrypcją za pomocą starterów genowo i regiono-specyficznych dla prekursora miRNA-944