ATE161586T1 - Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung - Google Patents

Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung

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Publication number
ATE161586T1
ATE161586T1 AT92309231T AT92309231T ATE161586T1 AT E161586 T1 ATE161586 T1 AT E161586T1 AT 92309231 T AT92309231 T AT 92309231T AT 92309231 T AT92309231 T AT 92309231T AT E161586 T1 ATE161586 T1 AT E161586T1
Authority
AT
Austria
Prior art keywords
polynucleotide
extender probe
sequence
target sequence
primer
Prior art date
Application number
AT92309231T
Other languages
English (en)
Inventor
Linda M Western
Karen M Hahnenberger
Samuel Rose
Martin Becker
Edwin F Ullman
Glenn H Mcgall
Original Assignee
Behringwerke Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke Ag filed Critical Behringwerke Ag
Application granted granted Critical
Publication of ATE161586T1 publication Critical patent/ATE161586T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT92309231T 1991-10-11 1992-10-09 Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung ATE161586T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77653891A 1991-10-11 1991-10-11
US92307092A 1992-07-31 1992-07-31

Publications (1)

Publication Number Publication Date
ATE161586T1 true ATE161586T1 (de) 1998-01-15

Family

ID=27119199

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92309231T ATE161586T1 (de) 1991-10-11 1992-10-09 Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung

Country Status (7)

Country Link
EP (1) EP0549107B1 (de)
JP (1) JPH07143900A (de)
AT (1) ATE161586T1 (de)
CA (1) CA2080305A1 (de)
DE (1) DE69223755T2 (de)
DK (1) DK0549107T3 (de)
ES (1) ES2112302T3 (de)

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US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
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US8017357B2 (en) 2000-04-07 2011-09-13 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
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CN112159836B (zh) * 2020-10-09 2022-09-06 中国科学院长春应用化学研究所 一种双发夹连接介导恒温扩增的核酸检测新方法

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Also Published As

Publication number Publication date
JPH07143900A (ja) 1995-06-06
DE69223755T2 (de) 1998-04-23
ES2112302T3 (es) 1998-04-01
DK0549107T3 (da) 1998-08-31
EP0549107A1 (de) 1993-06-30
EP0549107B1 (de) 1997-12-29
CA2080305A1 (en) 1993-04-12
DE69223755D1 (de) 1998-02-05

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