DE69223755T2 - Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung - Google Patents

Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung

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Publication number
DE69223755T2
DE69223755T2 DE69223755T DE69223755T DE69223755T2 DE 69223755 T2 DE69223755 T2 DE 69223755T2 DE 69223755 T DE69223755 T DE 69223755T DE 69223755 T DE69223755 T DE 69223755T DE 69223755 T2 DE69223755 T2 DE 69223755T2
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DE
Germany
Prior art keywords
polynucleotide
extender probe
sequence
target sequence
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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DE69223755T
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English (en)
Other versions
DE69223755D1 (de
Inventor
Linda M Western
Karen M Hahnenberger
Samuel Rose
Martin Becker
Edwin F Ullman
Glenn H Mcgall
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Siemens Healthcare Diagnostics GmbH Germany
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Behringwerke AG
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Publication of DE69223755D1 publication Critical patent/DE69223755D1/de
Publication of DE69223755T2 publication Critical patent/DE69223755T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69223755T 1991-10-11 1992-10-09 Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung Expired - Fee Related DE69223755T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77653891A 1991-10-11 1991-10-11
US92307092A 1992-07-31 1992-07-31

Publications (2)

Publication Number Publication Date
DE69223755D1 DE69223755D1 (de) 1998-02-05
DE69223755T2 true DE69223755T2 (de) 1998-04-23

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE69223755T Expired - Fee Related DE69223755T2 (de) 1991-10-11 1992-10-09 Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung

Country Status (7)

Country Link
EP (1) EP0549107B1 (de)
JP (1) JPH07143900A (de)
AT (1) ATE161586T1 (de)
CA (1) CA2080305A1 (de)
DE (1) DE69223755T2 (de)
DK (1) DK0549107T3 (de)
ES (1) ES2112302T3 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525462A (en) * 1991-05-02 1996-06-11 Toyo Boseki Kabushiki Kaisha Nucleic acid sequence amplification method, detection method, and reagent kit therefor
ZA929319B (en) * 1991-12-11 1993-05-24 Igen Inc Method for exponential amplification of nucleic acid by a single unpaired primer.
WO1995011923A1 (en) * 1993-10-29 1995-05-04 Dana-Farber Cancer Institute, Inc. A novel tumor marker and novel method of isolating same
US5480783A (en) * 1994-03-31 1996-01-02 The Perkin-Elmer Corporation Method for reducing background signals in DNA replication/detection assays
FR2721945B1 (fr) * 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
DE69610304T2 (de) 1995-05-05 2001-04-05 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
ES2279530T3 (es) * 1995-11-25 2007-08-16 Roche Diagnostics Gmbh Metodo para generar multiples acidos nucleicos bicatenarios.
US6326143B1 (en) 1996-11-22 2001-12-04 Roche Diagnostics Gmbh Method for generating multiple double stranded nucleic acids
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US20020048772A1 (en) 2000-02-10 2002-04-25 Dahiyat Bassil I. Protein design automation for protein libraries
US7315786B2 (en) 1998-10-16 2008-01-01 Xencor Protein design automation for protein libraries
JP3313358B2 (ja) 1998-11-09 2002-08-12 栄研化学株式会社 核酸の合成方法
US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
IL149446A0 (en) * 1999-11-08 2002-11-10 Eiken Chemical Method for synthesizing nucleic acid
EP1231281B1 (de) 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Methode zum nachweis von variationen oder polymorphismen
JP4721603B2 (ja) 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
US6800439B1 (en) 2000-01-06 2004-10-05 Affymetrix, Inc. Methods for improved array preparation
US6806361B1 (en) 2000-03-17 2004-10-19 Affymetrix, Inc. Methods of enhancing functional performance of nucleic acid arrays
US6833450B1 (en) 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
AU779160B2 (en) * 2000-03-28 2005-01-06 Eiken Kagaku Kabushiki Kaisha Method of synthesizing nucleic acids
AU2001244677A1 (en) 2000-04-07 2001-10-23 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US7005259B1 (en) 2000-06-01 2006-02-28 Affymetrix, Inc. Methods for array preparation using substrate rotation
DE10119203B4 (de) * 2001-04-19 2004-09-16 Rolf Dr. Postina Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung
EP1510959A3 (de) * 2001-08-10 2006-07-26 Xencor, Inc. Automatisierte Proteinkonstruktion für Proteinbibliotheken
CN1414110A (zh) * 2002-08-19 2003-04-30 张旭 利用具有3'至5'外切酶活性的多聚酶进行基因序列分析
DK1604044T3 (da) 2003-03-19 2009-08-31 Crescendo Biolog Ltd Genvinding af DNA
EP1929045B9 (de) * 2005-09-06 2012-06-20 Gen-Probe Incorporated Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
CN112159836B (zh) * 2020-10-09 2022-09-06 中国科学院长春应用化学研究所 一种双发夹连接介导恒温扩增的核酸检测新方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4663283A (en) 1980-03-24 1987-05-05 Genentech, Inc. Method of altering double-stranded DNA
US4358535A (en) 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
FI63596C (fi) 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4423153A (en) 1981-12-03 1983-12-27 Board Of Regents, The University Of Texas System Methods and compositions for the detection and determination of cellular DNA
US4480040A (en) 1981-12-03 1984-10-30 The United States Of America As Represented By The Secretary Of Agriculture Sensitive and rapid diagnosis of viroid diseases and viruses
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US4490472A (en) 1982-06-17 1984-12-25 Imreg, Inc. Sensitive tests for malignancies based on DNA detection
US4677054A (en) 1983-08-08 1987-06-30 Sloan-Kettering Institute For Cancer Research Method for simple analysis of relative nucleic acid levels in multiple small samples by cytoplasmic dot hybridization
US4599303A (en) 1983-12-12 1986-07-08 Hri Associates, Inc. Nucleic acid hybridization assay employing probes crosslinkable to target sequences
US4724202A (en) 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4647529A (en) 1984-06-01 1987-03-03 Rodland Karin D Hybridization method of detecting nucleic acid sequences with probe containing thionucleotide
US4675283A (en) 1984-07-19 1987-06-23 Massachusetts Institute Of Technology Detection and isolation of homologous, repeated and amplified nucleic acid sequences
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
CA1278755C (en) 1985-03-15 1991-01-08 William J. Knowles Labelling of oligonucleotides
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5008182A (en) 1986-01-10 1991-04-16 Cetus Corporation Detection of AIDS associated virus by polymerase chain reaction
GB8515276D0 (en) * 1985-06-17 1985-07-17 Amersham Int Plc Nucleic acid sequencing
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4910300A (en) 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4882269A (en) 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
BE904402A (fr) 1986-03-12 1986-09-12 Univ Libre De Bruxelles Iribhn Moule de sonde de detection de sequende d'adn et preparation d'un tel moule et d'une telle sonde.
US4908307A (en) 1986-12-19 1990-03-13 Karin D. Rodland Hybridization method and probe for detecting nucleic acid sequences
US5030557A (en) 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
IL90359A0 (en) 1988-05-26 1989-12-15 University Patents Inc Nucleoside and polynucleotide thiophosphoramidite and phosphorodithioate compounds and their production
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
CA2007431A1 (en) 1989-01-10 1990-07-10 Larry W. Mclauglin Labeling of nucleic acids with fluorescent markers
ATE142272T1 (de) 1989-01-19 1996-09-15 Behringwerke Ag Nukleinsäure-amplifikation unter verwendung eines einzelprimers
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification

Also Published As

Publication number Publication date
DK0549107T3 (da) 1998-08-31
ATE161586T1 (de) 1998-01-15
ES2112302T3 (es) 1998-04-01
EP0549107A1 (de) 1993-06-30
CA2080305A1 (en) 1993-04-12
EP0549107B1 (de) 1997-12-29
DE69223755D1 (de) 1998-02-05
JPH07143900A (ja) 1995-06-06

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Owner name: DADE BEHRING MARBURG GMBH, 35041 MARBURG, DE

8339 Ceased/non-payment of the annual fee