DE69223755T2 - Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung - Google Patents

Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung

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Publication number
DE69223755T2
DE69223755T2 DE69223755T DE69223755T DE69223755T2 DE 69223755 T2 DE69223755 T2 DE 69223755T2 DE 69223755 T DE69223755 T DE 69223755T DE 69223755 T DE69223755 T DE 69223755T DE 69223755 T2 DE69223755 T2 DE 69223755T2
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DE
Germany
Prior art keywords
polynucleotide
extender probe
sequence
target sequence
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69223755T
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English (en)
Other versions
DE69223755D1 (de
Inventor
Linda M Western
Karen M Hahnenberger
Samuel Rose
Martin Becker
Edwin F Ullman
Glenn H Mcgall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
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Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Publication of DE69223755D1 publication Critical patent/DE69223755D1/de
Application granted granted Critical
Publication of DE69223755T2 publication Critical patent/DE69223755T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
DE69223755T 1991-10-11 1992-10-09 Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung Expired - Fee Related DE69223755T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77653891A 1991-10-11 1991-10-11
US92307092A 1992-07-31 1992-07-31

Publications (2)

Publication Number Publication Date
DE69223755D1 DE69223755D1 (de) 1998-02-05
DE69223755T2 true DE69223755T2 (de) 1998-04-23

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE69223755T Expired - Fee Related DE69223755T2 (de) 1991-10-11 1992-10-09 Verfahren zur Herstellung eines Polynukleotids zum Gebrauch in "single-primer" Amplifizierung und Phosphorothioat-enthaltende Oligonukleotide als Primer in Nukleinsäureamplifizierung

Country Status (7)

Country Link
EP (1) EP0549107B1 (de)
JP (1) JPH07143900A (de)
AT (1) ATE161586T1 (de)
CA (1) CA2080305A1 (de)
DE (1) DE69223755T2 (de)
DK (1) DK0549107T3 (de)
ES (1) ES2112302T3 (de)

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US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
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EP2287338B1 (de) * 1998-11-09 2012-09-05 Eiken Kagaku Kabushiki Kaisha Prozess zur Synthetisierung von Nukleinsäure
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ATE317023T1 (de) * 1999-11-08 2006-02-15 Eiken Chemical Methode zum nachweis von variationen oder polymorphismen
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AU779160B2 (en) * 2000-03-28 2005-01-06 Eiken Kagaku Kabushiki Kaisha Method of synthesizing nucleic acids
ES2464132T3 (es) 2000-04-07 2014-05-30 Eiken Kagaku Kabushiki Kaisha Procedimiento de amplificación de ácido nucleico utilizando como molde ácido nucleico bicatenario
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CN1414110A (zh) * 2002-08-19 2003-04-30 张旭 利用具有3'至5'外切酶活性的多聚酶进行基因序列分析
EP1604044B1 (de) * 2003-03-19 2009-05-13 Discerna Limited Gewinnung von dna
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CN112159836B (zh) * 2020-10-09 2022-09-06 中国科学院长春应用化学研究所 一种双发夹连接介导恒温扩增的核酸检测新方法

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Also Published As

Publication number Publication date
DE69223755D1 (de) 1998-02-05
EP0549107B1 (de) 1997-12-29
DK0549107T3 (da) 1998-08-31
JPH07143900A (ja) 1995-06-06
ATE161586T1 (de) 1998-01-15
ES2112302T3 (es) 1998-04-01
EP0549107A1 (de) 1993-06-30
CA2080305A1 (en) 1993-04-12

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Owner name: DADE BEHRING MARBURG GMBH, 35041 MARBURG, DE

8339 Ceased/non-payment of the annual fee