DE602004021094D1 - Gewinnung von dna - Google Patents
Gewinnung von dnaInfo
- Publication number
- DE602004021094D1 DE602004021094D1 DE602004021094T DE602004021094T DE602004021094D1 DE 602004021094 D1 DE602004021094 D1 DE 602004021094D1 DE 602004021094 T DE602004021094 T DE 602004021094T DE 602004021094 T DE602004021094 T DE 602004021094T DE 602004021094 D1 DE602004021094 D1 DE 602004021094D1
- Authority
- DE
- Germany
- Prior art keywords
- mrna
- cdna
- methods
- gaining
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020004999 messenger RNA Proteins 0.000 abstract 5
- 239000002299 complementary DNA Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 238000010839 reverse transcription Methods 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 238000011084 recovery Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1041—Ribosome/Polysome display, e.g. SPERT, ARM
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Control Of Throttle Valves Provided In The Intake System Or In The Exhaust System (AREA)
- Valve Device For Special Equipments (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0306305A GB0306305D0 (en) | 2003-03-19 | 2003-03-19 | Recovery of DNA |
GB0311351A GB0311351D0 (en) | 2003-05-16 | 2003-05-16 | Activity-directed in vitro protein cloning and identification |
PCT/GB2004/001211 WO2004083461A1 (en) | 2003-03-19 | 2004-03-19 | Recovery of dna |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602004021094D1 true DE602004021094D1 (de) | 2009-06-25 |
Family
ID=33031411
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602004021094T Expired - Lifetime DE602004021094D1 (de) | 2003-03-19 | 2004-03-19 | Gewinnung von dna |
Country Status (9)
Country | Link |
---|---|
US (1) | US7572577B2 (da) |
EP (1) | EP1604044B1 (da) |
AT (1) | ATE431430T1 (da) |
CY (1) | CY1109285T1 (da) |
DE (1) | DE602004021094D1 (da) |
DK (1) | DK1604044T3 (da) |
ES (1) | ES2326649T3 (da) |
PT (1) | PT1604044E (da) |
WO (2) | WO2004083461A1 (da) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2752025T3 (es) | 2008-07-25 | 2020-04-02 | Wagner Richard W | Métodos de cribado de proteínas |
CN109476695A (zh) * | 2016-06-27 | 2019-03-15 | 丹娜法伯癌症研究院 | 用于测定rna翻译速率的方法 |
CN110904135B (zh) * | 2019-12-30 | 2021-08-13 | 苏州珀罗汀生物技术有限公司 | 蛋白质基础表达体系、合成系统及制备方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
ATE161586T1 (de) * | 1991-10-11 | 1998-01-15 | Behringwerke Ag | Verfahren zur herstellung eines polynukleotids zum gebrauch in ''single-primer'' amplifizierung und phosphorothioat-enthaltende oligonukleotide als primer in nukleinsäureamplifizierung |
DK0985032T4 (da) * | 1997-05-28 | 2009-08-31 | Crescendo Biolog Ltd | Ribosom-komplekser som selektionspartikler til in vitro fremvisning og evolution af proteiner |
US6207424B1 (en) | 1999-11-23 | 2001-03-27 | Maxim Biotech, Inc. | Self-primed amplification system |
US6379932B1 (en) | 2000-07-17 | 2002-04-30 | Incyte Genomics, Inc. | Single primer PCR amplification of RNA |
-
2004
- 2004-03-19 AT AT04721937T patent/ATE431430T1/de active
- 2004-03-19 US US10/549,778 patent/US7572577B2/en not_active Expired - Fee Related
- 2004-03-19 WO PCT/GB2004/001211 patent/WO2004083461A1/en active Application Filing
- 2004-03-19 DK DK04721937T patent/DK1604044T3/da active
- 2004-03-19 PT PT04721937T patent/PT1604044E/pt unknown
- 2004-03-19 DE DE602004021094T patent/DE602004021094D1/de not_active Expired - Lifetime
- 2004-03-19 WO PCT/GB2004/001206 patent/WO2004083428A1/en active Application Filing
- 2004-03-19 EP EP04721937A patent/EP1604044B1/en not_active Expired - Lifetime
- 2004-03-19 ES ES04721937T patent/ES2326649T3/es not_active Expired - Lifetime
-
2009
- 2009-08-11 CY CY20091100852T patent/CY1109285T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
DK1604044T3 (da) | 2009-08-31 |
US20060281086A1 (en) | 2006-12-14 |
PT1604044E (pt) | 2009-08-05 |
ES2326649T3 (es) | 2009-10-16 |
WO2004083461A1 (en) | 2004-09-30 |
WO2004083461A9 (en) | 2004-12-29 |
EP1604044A1 (en) | 2005-12-14 |
WO2004083428A1 (en) | 2004-09-30 |
CY1109285T1 (el) | 2014-07-02 |
EP1604044B1 (en) | 2009-05-13 |
US7572577B2 (en) | 2009-08-11 |
ATE431430T1 (de) | 2009-05-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2004092418A3 (en) | Global amplification using a randomly primed composite primer | |
WO2004074503A3 (en) | Nucleic acid sequencing methods, kits and reagents | |
WO2003062389A3 (en) | UNIVERSAL RT-COUPLED PCR METHOD FOR THE SPECIFIC AMPLIFICATION OF mRNA | |
DE112006002611A5 (de) | Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna | |
EP1845160A4 (en) | NUCLEOTIDE-amplification | |
DE60038796D1 (de) | Hochspezifische primer, amplifizierungsverfahren und kits | |
ATE488605T1 (de) | Verfahren zur quantitativen bewertung einer umordnung oder einer gezielten genetischen rekombination bei einem individuum sowie verwendungen davon | |
EP1350841A3 (en) | Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity | |
BR0101493B1 (pt) | mÉtodo para transcriÇço reversa e amplificaÇço de um rna usando dna polimerases mutantes. | |
DE602005023588D1 (de) | Verfahren zur genotypisierung von antigenen von blutzellen und ein kit zur genotypisierung | |
JP5200302B2 (ja) | ヘリカーゼを使用するrnaターゲットの同定 | |
DE50311731D1 (de) | Vermehrung von ribonukleinsäuren | |
ATE476523T1 (de) | Verfahren zur genomamplifikation | |
DK1654388T3 (da) | Fremgangsmåde til påvisning af cytosinmethyleringer i DNA | |
DE60309817D1 (de) | Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen | |
ATE455868T1 (de) | Polymerase-kettenreaktionsverfahren unter einsatz einer dna-polymerase mit proofreading- eigenschaften | |
CY1109285T1 (el) | Ανακτηση toy dna | |
ATE539167T1 (de) | Verfahren zur amplifikation von rna-sequenzen | |
ATE455866T1 (de) | Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität | |
DE502006008330D1 (de) | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna | |
WO2005070027A3 (en) | A novel nucleotide mixture for improved nucleic acid amplification performance | |
WO2005042764A3 (en) | Primers, methods and kits for amplifying or detecting human leukocyte antigen alleles | |
WO2008045431A3 (en) | Rapid reverse transcription of rna | |
DE50312117D1 (de) | Verfahren zur Amplifikation genetischer Information mittels an mehrere Stellen im Genom bindende Primer | |
WO2020056004A8 (en) | Methods and systems for multiplex gene amplification from ultra-low dna input amounts and uses thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8327 | Change in the person/name/address of the patent owner |
Owner name: CRESCENDO BIOLOGICS LTD., BABRAHAM, CAMBRIDGE, GB |
|
8364 | No opposition during term of opposition |