ATE481507T1 - Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv - Google Patents

Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv

Info

Publication number
ATE481507T1
ATE481507T1 AT05712046T AT05712046T ATE481507T1 AT E481507 T1 ATE481507 T1 AT E481507T1 AT 05712046 T AT05712046 T AT 05712046T AT 05712046 T AT05712046 T AT 05712046T AT E481507 T1 ATE481507 T1 AT E481507T1
Authority
AT
Austria
Prior art keywords
hcv
primer
detection
amplification
probe design
Prior art date
Application number
AT05712046T
Other languages
English (en)
Inventor
Hwa-Tang Wang
Brian Washburn
Original Assignee
Bio Merieux Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux Inc filed Critical Bio Merieux Inc
Application granted granted Critical
Publication of ATE481507T1 publication Critical patent/ATE481507T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT05712046T 2004-01-23 2005-01-21 Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv ATE481507T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US53881404P 2004-01-23 2004-01-23
US53881504P 2004-01-23 2004-01-23
US53881604P 2004-01-23 2004-01-23
PCT/US2005/002415 WO2005071117A2 (en) 2004-01-23 2005-01-21 Primer and probe design for efficient amplification and detection of hcv 3′ non-translating region

Publications (1)

Publication Number Publication Date
ATE481507T1 true ATE481507T1 (de) 2010-10-15

Family

ID=34812082

Family Applications (3)

Application Number Title Priority Date Filing Date
AT08075840T ATE491810T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung
AT05712046T ATE481507T1 (de) 2004-01-23 2005-01-21 Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv
AT05722543T ATE417938T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT08075840T ATE491810T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für eine verbesserte nukleinsäurenamplifikationsleistung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT05722543T ATE417938T1 (de) 2004-01-23 2005-01-21 Neuartiges nukleotidgemisch für ein verbessertes nukleinsäureamplifikationsverfahren

Country Status (6)

Country Link
US (3) US7364854B2 (de)
EP (3) EP2042612B1 (de)
JP (2) JP4866248B2 (de)
AT (3) ATE491810T1 (de)
DE (3) DE602005025424D1 (de)
WO (2) WO2005071117A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008134374A1 (en) * 2007-04-27 2008-11-06 Eragen Biosciences, Inc. Materials and methods for detection of hepatitis c virus
JP2009284896A (ja) * 2007-07-26 2009-12-10 Fujifilm Corp 核酸増幅方法
EP2058406A3 (de) * 2007-11-06 2009-10-28 Fujifilm Corporation RNA-Detektionsverfahren
JP2012513757A (ja) * 2008-12-31 2012-06-21 アボット・ラボラトリーズ Ｃ型肝炎ウイルスの検出用プライマー及びプローブ

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09107970A (ja) * 1995-10-17 1997-04-28 Soyaku Gijutsu Kenkyusho:Kk Ｃ型肝炎ウイルス由来核酸、該核酸を用いたウイルスの検出方法
EP0052296B1 (de) * 1980-11-12 1984-06-27 Mitsubishi Kasei Corporation Als Heilmittel für Immunokrankheiten anwendbare Glutaminderivate, Verfahren zu deren Herstellung und Zusammensetzungen, die die obengenannten Derivate enthalten
US5087565A (en) * 1984-07-02 1992-02-11 Du Pont Merck Pharmaceutical Company Synthetic process for preparation of 32 P-labeled nucleotides
JPH0723393B2 (ja) * 1985-05-15 1995-03-15 アモコ・コーポレーション シチジン同族体
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5032511A (en) * 1987-03-31 1991-07-16 Mitsubishi Kasei Corporation DNA fragments coding for antigens specific to non-A non-B hepatitis, expression vectors containing said DNA fragments, transformants and process for producing said antigens
CA1340843C (en) 1987-07-31 1999-12-07 J. Lawrence Burg Selective amplification of target polynucleotide sequences
US5698390A (en) * 1987-11-18 1997-12-16 Chiron Corporation Hepatitis C immunoassays
US5714596A (en) * 1987-11-18 1998-02-03 Chiron Corporation NANBV diagnostics: polynucleotides useful for screening for hepatitis C virus
US5712088A (en) * 1987-11-18 1998-01-27 Chiron Corporation Methods for detecting Hepatitis C virus using polynucleotides specific for same
US5030557A (en) 1987-11-24 1991-07-09 Ml Technology Venture Means and method for enhancing nucleic acid hybridization
JP2650159B2 (ja) 1988-02-24 1997-09-03 アクゾ・ノベル・エヌ・ベー 核酸増幅方法
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
EP0419180B1 (de) 1989-09-18 1994-08-03 Canon Kabushiki Kaisha Tintenstrahlaufzeichnungskopf und Tintenstrahlgerät, welches diesen aufweist
EP0515506B1 (de) * 1990-02-16 2000-01-05 F. Hoffmann-La Roche Ag Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
WO1993022461A1 (en) 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US5648232A (en) * 1993-01-21 1997-07-15 The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland Microbiological best method and reagents
JP3062250B2 (ja) * 1994-03-10 2000-07-10 ジェン−プローブ・インコーポレイテッド イオン性界面活性剤による、酵素により媒介される反応に対する阻害の抑制方法
US6127116A (en) * 1995-08-29 2000-10-03 Washington University Functional DNA clone for hepatitis C virus (HCV) and uses thereof
US5874565A (en) * 1995-08-29 1999-02-23 Washington University Nucleic acids comprising a highly conserved novel 3 terminal sequence element of the hepatitis C virus
DE19612779A1 (de) * 1996-03-29 1997-10-02 Boehringer Mannheim Gmbh Verfahren zur spezifischen Vervielfältigung von langen Nukleinsäuren durch PCR
WO1998006736A1 (en) * 1996-08-14 1998-02-19 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
DE19647055A1 (de) * 1996-11-14 1998-05-20 Boehringer Mannheim Gmbh Stabilisierte wässrige Nukleosidtriphosphat-Lösungen
EP2354251B1 (de) * 1997-04-03 2014-10-15 Life Technologies Corporation Verfahren und Produkte zur reversen Transkription gekoppelt mit Polymerasekettenreaktion (RT-PCR)
ZA989950B (en) * 1997-11-17 1999-05-04 Akzo Nobel Nv Transcription based amplification of double stranded DNA targets
EP1075508A2 (de) * 1998-04-27 2001-02-14 Ribozyme Pharmaceuticals, Inc. Enzymatische nukleinsäure zur hemmung der exprimierung und/oder replikation des hepatitis c-virus
WO2000000638A2 (en) 1998-06-26 2000-01-06 Akzo Nobel N.V. Tagging of rna amplicons generated by transcription-based amplification
US6638714B1 (en) 1999-02-03 2003-10-28 Ortho-Clinical Diagnostics, Inc. Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
DE60034884T2 (de) * 1999-06-04 2008-01-17 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Klonierte genome von infektiösen hepatitis c viren genotyp 2a und deren verwendungen
US6271004B1 (en) * 1999-06-25 2001-08-07 Display Systems Biotech A/S Method for improved reverse transcription at high temperatures
WO2001036442A1 (en) * 1999-11-17 2001-05-25 Jiuping Ji Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
AU2001254670A1 (en) 2000-03-02 2001-09-12 Akzo Nobel N.V. Detection of hepatitis b virus rna
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication
WO2003016572A1 (en) * 2001-08-17 2003-02-27 Eli Lilly And Company Oligonucleotide therapeutics for treating hepatitis c virus infections
US6783940B2 (en) * 2001-10-31 2004-08-31 Applera Corporation Method of reducing non-specific amplification in PCR
US6750009B2 (en) 2002-01-29 2004-06-15 Apath, Llc Multiple viral replicon culture systems
AU2003241614A1 (en) 2002-05-24 2003-12-12 Smithkline Beecham Corporation Method for quantitating negative strand rna synthesis

Also Published As

Publication number Publication date
ATE491810T1 (de) 2011-01-15
ATE417938T1 (de) 2009-01-15
WO2005070027A8 (en) 2006-07-27
JP2007518425A (ja) 2007-07-12
EP1706512A2 (de) 2006-10-04
WO2005070027A2 (en) 2005-08-04
WO2005071117A3 (en) 2006-04-20
US7670779B2 (en) 2010-03-02
EP1721015A2 (de) 2006-11-15
US20060019266A1 (en) 2006-01-26
WO2005070027A3 (en) 2006-04-06
US20060003343A1 (en) 2006-01-05
EP1706512B1 (de) 2008-12-17
DE602005023586D1 (de) 2010-10-28
EP2042612A2 (de) 2009-04-01
US20080248480A1 (en) 2008-10-09
JP4866248B2 (ja) 2012-02-01
EP1721015B1 (de) 2010-09-15
WO2005071117A2 (en) 2005-08-04
US7364854B2 (en) 2008-04-29
DE602005025424D1 (de) 2011-01-27
EP2042612B1 (de) 2010-12-15
EP2042612A3 (de) 2009-06-17
DE602005011754D1 (de) 2009-01-29
JP2007518424A (ja) 2007-07-12

Similar Documents

Publication Publication Date Title
DE60041940D1 (de) Oligonukleotidprimer zur effizienten Detektion von Hepatitis C Virus (HCV) sowie Verwendungsverfahren
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
DE60235005D1 (de) Nachweis der instabilität von mikrosatelliten und
HK1074458A1 (en) Thermostable taq polymerase fragment
NZ596545A (en) Improved detection of mage-a expression
ATE484515T1 (de) Dna-polymerase-mischungen und mutante dna- polymerasen
DE60020143D1 (de) Typisierung von humanen enteroviren
ATE481507T1 (de) Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv
ATE386821T1 (de) Zusammensetzungen und methoden zur detektion von humanem immundefizienz virus 2 (hiv-2)
ATE469162T1 (de) Verfahren und zusammensetzungen zur identifizierung und charakterisierung von hepatitis c
Lee et al. Development of molecular diagnostic system with high sensitivity for the detection of human Sapovirus from water environments
DE50208138D1 (de) Verfahren zur quantitiven Bestimmung des Methylierungsgrades von Cytosinen in CpG-Positionen
AR027256A1 (es) Deteccion de microorganismos relacionados con la fermentacion
DE60108337D1 (de) Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
WO2004043981A3 (en) Nucleic acids and proteins with thioredoxin reductase activity
ATE431430T1 (de) Gewinnung von dna
DE602006017973D1 (de) Thermostabile DNA Polymerase von archae Ampullavirus ABV und dessen Verwendungen
DE60212861D1 (de) Verfahren zur Diagnose von Fettsucht
ATE407145T1 (de) Analyse und verwendung von par 1 polymorphismen zur risikoabschätzung für kardiovaskuläre erkrankungen
ATE328119T1 (de) Nachweis von variola virus

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties