ATE541055T1 - Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation - Google Patents

Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation

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Publication number
ATE541055T1
ATE541055T1 AT05813115T AT05813115T ATE541055T1 AT E541055 T1 ATE541055 T1 AT E541055T1 AT 05813115 T AT05813115 T AT 05813115T AT 05813115 T AT05813115 T AT 05813115T AT E541055 T1 ATE541055 T1 AT E541055T1
Authority
AT
Austria
Prior art keywords
pcr amplification
reagents
pcr
methods
amplifications
Prior art date
Application number
AT05813115T
Other languages
English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Original Assignee
Univ Brandeis
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Publication date
Application filed by Univ Brandeis filed Critical Univ Brandeis
Application granted granted Critical
Publication of ATE541055T1 publication Critical patent/ATE541055T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05813115T 2004-10-18 2005-10-17 Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation ATE541055T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61967004P 2004-10-18 2004-10-18
PCT/US2005/037668 WO2006044995A1 (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Publications (1)

Publication Number Publication Date
ATE541055T1 true ATE541055T1 (de) 2012-01-15

Family

ID=36203307

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05813115T ATE541055T1 (de) 2004-10-18 2005-10-17 Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation

Country Status (14)

Country Link
US (2) US7517977B2 (de)
EP (1) EP1802774B1 (de)
JP (2) JP5165376B2 (de)
KR (1) KR20070090158A (de)
CN (1) CN101076608B (de)
AT (1) ATE541055T1 (de)
AU (1) AU2005295299B2 (de)
BR (1) BRPI0516619B1 (de)
CA (1) CA2584576C (de)
ES (1) ES2380775T3 (de)
NO (1) NO341716B1 (de)
NZ (1) NZ554701A (de)
RU (1) RU2495046C2 (de)
WO (1) WO2006044995A1 (de)

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US7517977B2 (en) * 2004-10-18 2009-04-14 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
WO2007041201A2 (en) * 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
WO2010105074A1 (en) * 2009-03-12 2010-09-16 Brandeis University Reagents and methods for pcr
EP2412718B1 (de) 2009-03-26 2016-10-12 Xiamen Amoy Diagnostics Co., Ltd Bei der nukleinsäureamplifikation eingesetzter schleifenförmiger primer und dessen anwendung
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
ES2549777T3 (es) 2009-10-21 2015-11-02 Brandeis University Métodos para analizar secuencias de ácido nucleico monocatenario
CN103459612A (zh) 2010-12-03 2013-12-18 布兰代斯大学 用于检测野生型群体中的核酸突变体的方法和试剂盒
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
CA2898613A1 (en) 2013-01-23 2014-07-31 Brandeis University Reagents for improving pcr accuracy
US20160355881A1 (en) * 2013-07-24 2016-12-08 Brandeis University DNA Methylation Analysis
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
EP3901278A1 (de) * 2014-05-19 2021-10-27 William Marsh Rice University Allelspezifische amplifizierung mit einer zusammensetzung aus überlappendem nicht-allelspezifischem primer und allelspezifischen blockeroligonukleotiden
US9477198B2 (en) 2014-09-03 2016-10-25 Samsung Electronics Co., Ltd. Cleaning device and electrophotographic image forming apparatus having the same
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
EP3234186A1 (de) 2014-12-19 2017-10-25 Brandeis University Reagenzien zur verhinderung von fehlerhaftem priming
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
US20190309349A1 (en) * 2016-05-18 2019-10-10 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
KR20220151011A (ko) 2016-09-23 2022-11-11 알베오 테크놀로지스 인크. 분석물을 검출하기 위한 방법 및 조성물
CA3123839A1 (en) * 2018-12-20 2020-06-25 Alveo Technologies, Inc. Methods and compositions to reduce nonspecific amplification in isothermal amplification reactions
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트

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Also Published As

Publication number Publication date
BRPI0516619B1 (pt) 2016-02-10
KR20070090158A (ko) 2007-09-05
CN101076608B (zh) 2013-04-17
US20090226973A1 (en) 2009-09-10
CA2584576A1 (en) 2006-04-27
AU2005295299A1 (en) 2006-04-27
CN101076608A (zh) 2007-11-21
NO20072507L (no) 2007-07-06
BRPI0516619A (pt) 2008-09-16
AU2005295299B2 (en) 2010-10-21
RU2495046C2 (ru) 2013-10-10
WO2006044995A1 (en) 2006-04-27
JP5165376B2 (ja) 2013-03-21
JP2013063070A (ja) 2013-04-11
ES2380775T3 (es) 2012-05-18
JP2008516613A (ja) 2008-05-22
EP1802774B1 (de) 2012-01-11
EP1802774A1 (de) 2007-07-04
NZ554701A (en) 2010-03-26
EP1802774A4 (de) 2008-10-01
RU2007118546A (ru) 2008-11-27
NO341716B1 (no) 2018-01-08
CA2584576C (en) 2015-12-08
US7517977B2 (en) 2009-04-14
US20060177842A1 (en) 2006-08-10

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