BRPI0516619A - reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr - Google Patents

reagentes e métodos para reprodutibilidade aperfeiçoada e redução de iniciação errÈnia em amplificação por pcr

Info

Publication number
BRPI0516619A
BRPI0516619A BRPI0516619-5A BRPI0516619A BRPI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A BR PI0516619 A BRPI0516619 A BR PI0516619A
Authority
BR
Brazil
Prior art keywords
reagents
pcr amplification
reduction
methods
pcr
Prior art date
Application number
BRPI0516619-5A
Other languages
English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Original Assignee
Univ Brandeis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brandeis filed Critical Univ Brandeis
Publication of BRPI0516619A publication Critical patent/BRPI0516619A/pt
Publication of BRPI0516619B1 publication Critical patent/BRPI0516619B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

REAGENTES E MéTODOS PARA REPRODUTIBILIDADE APERFEIçOADA E REDUçãO DE INICIAçãO ERRÈNEA EM AMPLIFICAçãO POR PCR. Um aditivo para prevenção de iniciação errónea em amplificações por reação em cadeia de polimerase (PCR) e ensaios compreendendo um oligonucleotídeo de hairpin tendo uma dupla de tronco maior do que seis nucleotídeos de comprimento e um término de tronco estabilizado. O aditivo melhora as amplificações por PCR, incluindo amplificações por LATE-PCR, quando adicionado às misturas de reação de amplificação iniciais. Ele pode ser incluído em conjuntos de oligonucleotídeo e em kits para amplificação e ensaios por PCR.
BRPI0516619A 2004-10-18 2005-10-17 reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase BRPI0516619B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61967004P 2004-10-18 2004-10-18
PCT/US2005/037668 WO2006044995A1 (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Publications (2)

Publication Number Publication Date
BRPI0516619A true BRPI0516619A (pt) 2008-09-16
BRPI0516619B1 BRPI0516619B1 (pt) 2016-02-10

Family

ID=36203307

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0516619A BRPI0516619B1 (pt) 2004-10-18 2005-10-17 reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase

Country Status (14)

Country Link
US (2) US7517977B2 (pt)
EP (1) EP1802774B1 (pt)
JP (2) JP5165376B2 (pt)
KR (1) KR20070090158A (pt)
CN (1) CN101076608B (pt)
AT (1) ATE541055T1 (pt)
AU (1) AU2005295299B2 (pt)
BR (1) BRPI0516619B1 (pt)
CA (1) CA2584576C (pt)
ES (1) ES2380775T3 (pt)
NO (1) NO341716B1 (pt)
NZ (1) NZ554701A (pt)
RU (1) RU2495046C2 (pt)
WO (1) WO2006044995A1 (pt)

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* Cited by examiner, † Cited by third party
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US7517977B2 (en) * 2004-10-18 2009-04-14 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
CN101321877B (zh) * 2005-10-03 2013-04-10 应用生物系统有限责任公司 用于扩增核酸的组合物、方法和试剂盒
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
EP2406394B1 (en) * 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr
US9644233B2 (en) 2009-03-26 2017-05-09 Xiamen Amoy Diagnostics Co., Ltd. Loop-shaped primer used in nucleic acid amplification and the use thereof
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
WO2011050173A1 (en) 2009-10-21 2011-04-28 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
WO2012075231A1 (en) 2010-12-03 2012-06-07 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
JP6490576B2 (ja) * 2012-04-12 2019-03-27 ニユー・イングランド・バイオレイブス・インコーポレイテツド 重合反応のための合成核酸
US10301688B2 (en) * 2013-01-23 2019-05-28 Brandeis University Reagents for improving PCR accuracy
WO2015013521A1 (en) * 2013-07-24 2015-01-29 Brandeis University Dna methylation analysis
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
CN106661627B (zh) * 2014-05-19 2021-11-23 威廉马歇莱思大学 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
US9477198B2 (en) 2014-09-03 2016-10-25 Samsung Electronics Co., Ltd. Cleaning device and electrophotographic image forming apparatus having the same
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
JP2017537648A (ja) 2014-12-19 2017-12-21 ブランデイズ ユニバーシティー ミスプライミング防止試薬
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
CA3023256A1 (en) * 2016-05-18 2017-11-23 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
KR102461735B1 (ko) 2016-09-23 2022-11-01 알베오 테크놀로지스 인크. 분석물을 검출하기 위한 방법 및 조성물
JP2022515192A (ja) * 2018-12-20 2022-02-17 アルヴェオ テクノロジーズ インコーポレイテッド 等温増幅反応における非特異的増幅を低減するための方法および組成物

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Also Published As

Publication number Publication date
RU2495046C2 (ru) 2013-10-10
CA2584576A1 (en) 2006-04-27
EP1802774A4 (en) 2008-10-01
RU2007118546A (ru) 2008-11-27
ES2380775T3 (es) 2012-05-18
US20090226973A1 (en) 2009-09-10
JP2008516613A (ja) 2008-05-22
US7517977B2 (en) 2009-04-14
CN101076608B (zh) 2013-04-17
JP2013063070A (ja) 2013-04-11
ATE541055T1 (de) 2012-01-15
EP1802774A1 (en) 2007-07-04
US20060177842A1 (en) 2006-08-10
NO20072507L (no) 2007-07-06
EP1802774B1 (en) 2012-01-11
NZ554701A (en) 2010-03-26
BRPI0516619B1 (pt) 2016-02-10
KR20070090158A (ko) 2007-09-05
AU2005295299A1 (en) 2006-04-27
CN101076608A (zh) 2007-11-21
AU2005295299B2 (en) 2010-10-21
JP5165376B2 (ja) 2013-03-21
NO341716B1 (no) 2018-01-08
WO2006044995A1 (en) 2006-04-27
CA2584576C (en) 2015-12-08

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 10/02/2016, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2433 DE 22-08-2017 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.