FI953079A0 - DNA:n vahvistaminen - Google Patents

DNA:n vahvistaminen

Info

Publication number
FI953079A0
FI953079A0 FI953079A FI953079A FI953079A0 FI 953079 A0 FI953079 A0 FI 953079A0 FI 953079 A FI953079 A FI 953079A FI 953079 A FI953079 A FI 953079A FI 953079 A0 FI953079 A0 FI 953079A0
Authority
FI
Finland
Prior art keywords
sequence
dna sequence
dna
genome
cell
Prior art date
Application number
FI953079A
Other languages
English (en)
Swedish (sv)
Other versions
FI120741B (fi
FI953079A (fi
Inventor
Steen Troels Joergensen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI953079A0 publication Critical patent/FI953079A0/fi
Publication of FI953079A publication Critical patent/FI953079A/fi
Application granted granted Critical
Publication of FI120741B publication Critical patent/FI120741B/fi

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Semiconductor Lasers (AREA)
FI953079A 1992-12-22 1995-06-21 DNA:n vahvistaminen FI120741B (fi)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK153992 1992-12-22
DK921539A DK153992D0 (da) 1992-12-22 1992-12-22 Metode
DK9300438 1993-12-22
PCT/DK1993/000438 WO1994014968A1 (en) 1992-12-22 1993-12-22 Dna amplification
CN94109005A CN1063488C (zh) 1992-12-22 1994-06-22 Dna扩增
CN94109005 1994-06-22

Publications (3)

Publication Number Publication Date
FI953079A0 true FI953079A0 (fi) 1995-06-21
FI953079A FI953079A (fi) 1995-06-21
FI120741B FI120741B (fi) 2010-02-15

Family

ID=37075831

Family Applications (1)

Application Number Title Priority Date Filing Date
FI953079A FI120741B (fi) 1992-12-22 1995-06-21 DNA:n vahvistaminen

Country Status (11)

Country Link
EP (1) EP0675959B1 (fi)
JP (1) JP3983798B2 (fi)
CN (1) CN1063488C (fi)
AT (1) ATE323167T1 (fi)
AU (1) AU679707B2 (fi)
CA (1) CA2152371C (fi)
DE (1) DE69334006T2 (fi)
DK (2) DK153992D0 (fi)
FI (1) FI120741B (fi)
NO (1) NO952496L (fi)
WO (1) WO1994014968A1 (fi)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2158364A1 (en) * 1993-03-15 1994-09-29 Daniel G. Brenner Method for defined deletions of dna
WO1996029418A1 (en) * 1995-03-22 1996-09-26 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2762842B1 (fr) 1997-05-05 1999-07-23 Pasteur Institut Procede de detection de la presence de matieres biologiques d'origine bovine, et oligonucleotides pour sa mise en oeuvre
EP1002109A1 (en) * 1997-07-03 2000-05-24 Novo Nordisk A/S Protein producing cell containing multiple copies of a desired gene and a screenable marker but no selection marker
ATE293359T1 (de) 1997-12-22 2005-05-15 Novozymes As Kohlenhydratoxidase sowie verwendung derselben beim backen
EP1297170B1 (en) 2000-06-23 2016-03-02 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
ATE522614T1 (de) 2002-04-10 2011-09-15 Novozymes As Mutierte bacillus licheniformis wirtszelle
WO2003087149A2 (en) 2002-04-10 2003-10-23 Novozymes A/S Bacillus licheniformis mutant host cell
CN1902318A (zh) 2003-10-31 2007-01-24 诺维信公司 细菌宿主细胞中稳定地基因扩增的方法
AU2004303639B2 (en) * 2003-11-25 2007-05-17 Japan Science And Technology Agency Gene amplification method
CN101006174B (zh) 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
EP1967585B1 (en) * 2005-11-24 2011-12-28 Inter-University Research Institute Corporation National Institutes of Natural Sciences Method for gene amplification
DK2089516T3 (da) 2006-11-29 2012-02-20 Novozymes Inc Fremgangsmåder til at forbedre indføringen af DNA i bakterielle celler
DK2104739T3 (da) 2006-12-21 2013-10-07 Novozymes Inc Modificerede messenger-RNA-stabiliseringssekvenser til ekspression af gener i bakterieceller
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
US20140141471A1 (en) 2011-05-19 2014-05-22 Novozymes, Inc. Methods for Enhancing the Degradation of Cellulosic Material with Chitin Binding Proteins
EP3532619A1 (en) 2016-10-25 2019-09-04 Novozymes A/S Flp-mediated genomic integration
CN107287331B (zh) * 2017-08-01 2020-06-26 北京大学 一种高效检测蓝藻细胞周期的方法
EP3707252A1 (en) 2017-11-10 2020-09-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
WO2020156903A1 (en) 2019-01-30 2020-08-06 Novozymes A/S Cognate foldase co-expression
EP3935160A1 (en) 2019-03-08 2022-01-12 Novozymes A/S Means and methods for improving protease expression
EP3969576A1 (en) 2019-05-15 2022-03-23 Novozymes A/S Temperature-sensitive rna-guided endonuclease
US20220298517A1 (en) 2019-06-25 2022-09-22 Novozymes A/S Counter-selection by inhibition of conditionally essential genes
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
AU645294B2 (en) * 1989-12-22 1994-01-13 Merck Serono Sa Endogenous gene expression modification with regulatory element

Also Published As

Publication number Publication date
DK153992D0 (da) 1992-12-22
CN1063488C (zh) 2001-03-21
DK0675959T3 (da) 2006-08-07
DE69334006T2 (de) 2006-11-23
JPH08504585A (ja) 1996-05-21
ATE323167T1 (de) 2006-04-15
AU679707B2 (en) 1997-07-10
EP0675959A1 (en) 1995-10-11
CN1114355A (zh) 1996-01-03
WO1994014968A1 (en) 1994-07-07
DE69334006D1 (de) 2006-05-24
AU5694494A (en) 1994-07-19
JP3983798B2 (ja) 2007-09-26
CA2152371A1 (en) 1994-07-07
FI120741B (fi) 2010-02-15
NO952496D0 (no) 1995-06-21
EP0675959B1 (en) 2006-04-12
NO952496L (no) 1995-08-21
CA2152371C (en) 2008-12-02
FI953079A (fi) 1995-06-21

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