ATE502111T1 - Auf replizierende vektoren basierende genexpression in plastiden - Google Patents

Auf replizierende vektoren basierende genexpression in plastiden

Info

Publication number
ATE502111T1
ATE502111T1 AT02732689T AT02732689T ATE502111T1 AT E502111 T1 ATE502111 T1 AT E502111T1 AT 02732689 T AT02732689 T AT 02732689T AT 02732689 T AT02732689 T AT 02732689T AT E502111 T1 ATE502111 T1 AT E502111T1
Authority
AT
Austria
Prior art keywords
sequence
interest
selection marker
plastids
plant
Prior art date
Application number
AT02732689T
Other languages
English (en)
Inventor
Hans-Ulrich Koop
Stefan Muehlbauer
Sebastian Klaus
Christian Eibl
Fong-Chin Huang
Timothy Golds
Original Assignee
Icon Genetics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Icon Genetics Gmbh filed Critical Icon Genetics Gmbh
Application granted granted Critical
Publication of ATE502111T1 publication Critical patent/ATE502111T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Magnetic Ceramics (AREA)
AT02732689T 2001-07-06 2002-04-30 Auf replizierende vektoren basierende genexpression in plastiden ATE502111T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10132780A DE10132780A1 (de) 2001-07-06 2001-07-06 Plastidäre Genexpression über autonom replizierende Vektoren
PCT/EP2002/004777 WO2003004658A2 (en) 2001-07-06 2002-04-30 Gene expression in plastids based on replicating vectors

Publications (1)

Publication Number Publication Date
ATE502111T1 true ATE502111T1 (de) 2011-04-15

Family

ID=7690826

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02732689T ATE502111T1 (de) 2001-07-06 2002-04-30 Auf replizierende vektoren basierende genexpression in plastiden

Country Status (9)

Country Link
US (1) US7371923B2 (de)
EP (1) EP1404848B1 (de)
JP (1) JP4340148B2 (de)
AT (1) ATE502111T1 (de)
AU (1) AU2002304711B2 (de)
CA (1) CA2453023C (de)
DE (2) DE10132780A1 (de)
MX (1) MXPA03011874A (de)
WO (1) WO2003004658A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10049587A1 (de) * 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143238A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143205A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Verfahren zur Proteinproduktion in Pflanzen
DE10143237A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
BR112020011670A2 (pt) 2017-12-15 2020-11-17 Flagship Pioneering Innovations Vi, Llc. composições compreendendo polirribonucleotídeos circulares e seus usos

Family Cites Families (50)

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AU6137986A (en) 1985-07-16 1987-02-10 Salk Institute For Biological Studies, The Genetic alteration of plants with retroviruses
US6147278A (en) 1985-10-25 2000-11-14 Monsanto Company Plant vectors
GB8626878D0 (en) 1986-11-11 1986-12-10 Ici Plc Dna
US5922602A (en) 1988-02-26 1999-07-13 Biosource Technologies, Inc. Cytoplasmic inhibition of gene expression
US6174700B1 (en) 1988-07-08 2001-01-16 University Of British Columbia Purification of a polypeptide compound having a polysaccharide binding domain by affinity phase separation
FR2649518B1 (fr) 1989-07-07 1991-10-18 Bioprobe Systems Sa Procede et dispositif de marquage crypte de haute securite pour la protection d'objets de valeur
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5474925A (en) 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber
ATE234361T1 (de) 1992-12-30 2003-03-15 Biosource Genetics Corp Virale amplifikation rekombinanter boten-rna in transgenen pflanzen
US5496934A (en) 1993-04-14 1996-03-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acids encoding a cellulose binding domain
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5723765A (en) 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
NZ296197A (en) 1994-12-08 1999-11-29 Pabio Chemical labelling of objects or material; at least two chemical tags added, first not divulged to public, second indicates presence of first
US6037525A (en) 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
JP2001500009A (ja) 1996-09-09 2001-01-09 ロヨラ ユニバーシティ オブ シカゴ 植物レトロウイルスポリヌクレオチドおよびその使用のための方法
US5939541A (en) 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
FI972293A (fi) 1997-05-30 1998-12-01 Joseph Atabekov Useamman kuin yhden geenin yhteisekspressiomenetelmät
DK1034262T3 (da) 1997-11-18 2005-11-28 Pioneer Hi Bred Int Sammensætninger og fremgangsmåder til genetisk modifikation af planter
ES2338284T3 (es) 1997-11-18 2010-05-05 Pioneer Hi-Bred International, Inc. Movilizacion de genomas viricos a partir de t-adn utilizando sistemas de recombinacion especificos de sitio.
WO1999036516A2 (en) 1998-01-16 1999-07-22 Biosource Technologies, Inc. Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
CA2339595A1 (en) 1998-09-23 2000-03-30 Narendra S. Yadav Binary viral expression system in plants
GB9821303D0 (en) 1998-10-01 1998-11-25 Novartis Ag Organic compounds
WO2000068391A1 (en) 1998-10-23 2000-11-16 University Of British Columbia Expression of a mannan binding domain to alter plant morphology
JP2002531096A (ja) 1998-11-25 2002-09-24 カルジーン エルエルシー 色素体を形質転換するための方法
EP1045037A1 (de) 1999-04-12 2000-10-18 Discovery Biotech., Inc. System zu Identifizierung von Planzenzellen
ATE347617T1 (de) 1999-05-06 2006-12-15 Sinai School Medicine Steganographie auf dna basis
MXPA01011541A (es) 1999-05-17 2002-06-04 Icon Genetics Inc Proceso para una rapida transformacion de plantas independientemente de la variedad.
US6331416B1 (en) 1999-06-10 2001-12-18 Cbd Technologies Ltd. Process of expressing and isolating recombinant proteins and recombinant protein products from plants, plant derived tissues or cultured plant cells
CA2375831A1 (en) 1999-06-24 2000-12-28 Metabolix, Inc. Plant multi-gene expression constructs
ATE324434T1 (de) 1999-08-05 2006-05-15 Icon Genetics Inc Verfahren zur herstellung künstlicher pflanzenchromosomen
AU2001242360A1 (en) 2000-02-10 2001-08-20 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Plant internal ribosome entry segment
US20030188337A1 (en) * 2000-04-20 2003-10-02 Anil Day Transgenic plants
AU2001257300A1 (en) * 2000-04-26 2001-11-07 Monsanto Technology Llc Method for the transformation of plant cell plastids
GB0019745D0 (en) 2000-08-10 2000-09-27 Univ Surrey Gene expression element
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10101276A1 (de) 2001-01-12 2002-07-18 Icon Genetics Ag Verfahren und Vektoren zur Transformation von Plastiden
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10109354A1 (de) 2001-02-27 2002-09-05 Icon Genetics Ag Rekombinante virale Schaltersysteme
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10129010A1 (de) 2001-06-13 2002-12-19 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10131680A1 (de) 2001-06-29 2003-01-23 Voith Paper Patent Gmbh Auftragsvorrichtung
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DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143205A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Verfahren zur Proteinproduktion in Pflanzen

Also Published As

Publication number Publication date
JP2004533266A (ja) 2004-11-04
US20050015829A1 (en) 2005-01-20
AU2002304711B2 (en) 2007-04-05
MXPA03011874A (es) 2004-06-03
DE10132780A1 (de) 2003-01-16
DE60239462D1 (de) 2011-04-28
EP1404848B1 (de) 2011-03-16
US7371923B2 (en) 2008-05-13
EP1404848A2 (de) 2004-04-07
JP4340148B2 (ja) 2009-10-07
CA2453023A1 (en) 2003-01-16
WO2003004658A2 (en) 2003-01-16
CA2453023C (en) 2011-09-20
WO2003004658A3 (en) 2004-01-08

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