MXPA03011874A - Expresion genica en plastidas basadas en vectores replicantes. - Google Patents

Expresion genica en plastidas basadas en vectores replicantes.

Info

Publication number
MXPA03011874A
MXPA03011874A MXPA03011874A MXPA03011874A MXPA03011874A MX PA03011874 A MXPA03011874 A MX PA03011874A MX PA03011874 A MXPA03011874 A MX PA03011874A MX PA03011874 A MXPA03011874 A MX PA03011874A MX PA03011874 A MXPA03011874 A MX PA03011874A
Authority
MX
Mexico
Prior art keywords
sequence
interest
selection marker
plastids
plant
Prior art date
Application number
MXPA03011874A
Other languages
English (en)
Inventor
Sebastian Klaus
Original Assignee
Icon Genetics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Icon Genetics Ag filed Critical Icon Genetics Ag
Publication of MXPA03011874A publication Critical patent/MXPA03011874A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Magnetic Ceramics (AREA)

Abstract

Un proceso para generar plantas transgenicas o celulas de planta transformadas en su plastoma y que estan desprovistos de un marcador de seleccion, que comprende los siguientes pasos: (a) transformar las plastidas de una planta o celula de planta con un ADN que comprende: (i) una secuencia de nucleotidos que confiere replicacion del ADN en una celula de planta, (ii) al menos una secuencia de interes (iii) secuencias que flanquean al menos una secuencia de interes necesaria la integracion estable de al menos una secuencia de interes en el genoma de la plastida, y (iv) un marcador de seleccion fuera de las secuencias que flanquean la (s) secuencia (s) de interes; (b) permitir la integracion de al menos una secuencia de interes en el plastoma en la presencia de presion selectiva; (6) permitir la perdida de la secuencia del marcador de seleccion al liberar la presion selectiva; y (d) recuperar las celulas y/o plantas que esten geneticamente transformadas en sus plastomas y que esten desprovistas del marcador de seleccion.
MXPA03011874A 2001-07-06 2002-04-30 Expresion genica en plastidas basadas en vectores replicantes. MXPA03011874A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10132780A DE10132780A1 (de) 2001-07-06 2001-07-06 Plastidäre Genexpression über autonom replizierende Vektoren
PCT/EP2002/004777 WO2003004658A2 (en) 2001-07-06 2002-04-30 Gene expression in plastids based on replicating vectors

Publications (1)

Publication Number Publication Date
MXPA03011874A true MXPA03011874A (es) 2004-06-03

Family

ID=7690826

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA03011874A MXPA03011874A (es) 2001-07-06 2002-04-30 Expresion genica en plastidas basadas en vectores replicantes.

Country Status (9)

Country Link
US (1) US7371923B2 (es)
EP (1) EP1404848B1 (es)
JP (1) JP4340148B2 (es)
AT (1) ATE502111T1 (es)
AU (1) AU2002304711B2 (es)
CA (1) CA2453023C (es)
DE (2) DE10132780A1 (es)
MX (1) MXPA03011874A (es)
WO (1) WO2003004658A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10049587A1 (de) 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143205A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Verfahren zur Proteinproduktion in Pflanzen
DE10143238A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
CN111819185A (zh) 2017-12-15 2020-10-23 旗舰创业创新第六有限责任公司 包含环状多核糖核苷酸的组合物及其用途

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DE10143237A1 (de) 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
DE10143205A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Verfahren zur Proteinproduktion in Pflanzen

Also Published As

Publication number Publication date
CA2453023C (en) 2011-09-20
US20050015829A1 (en) 2005-01-20
US7371923B2 (en) 2008-05-13
EP1404848A2 (en) 2004-04-07
ATE502111T1 (de) 2011-04-15
CA2453023A1 (en) 2003-01-16
DE60239462D1 (de) 2011-04-28
DE10132780A1 (de) 2003-01-16
EP1404848B1 (en) 2011-03-16
WO2003004658A3 (en) 2004-01-08
JP4340148B2 (ja) 2009-10-07
JP2004533266A (ja) 2004-11-04
AU2002304711B2 (en) 2007-04-05
WO2003004658A2 (en) 2003-01-16

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