FI953079A0 - Förstärkning av DNA - Google Patents

Förstärkning av DNA

Info

Publication number
FI953079A0
FI953079A0 FI953079A FI953079A FI953079A0 FI 953079 A0 FI953079 A0 FI 953079A0 FI 953079 A FI953079 A FI 953079A FI 953079 A FI953079 A FI 953079A FI 953079 A0 FI953079 A0 FI 953079A0
Authority
FI
Finland
Prior art keywords
sequence
dna sequence
dna
genome
cell
Prior art date
Application number
FI953079A
Other languages
English (en)
Finnish (fi)
Other versions
FI953079A (sv
FI120741B (sv
Inventor
Steen Troels Joergensen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of FI953079A publication Critical patent/FI953079A/sv
Publication of FI953079A0 publication Critical patent/FI953079A0/sv
Application granted granted Critical
Publication of FI120741B publication Critical patent/FI120741B/sv

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Semiconductor Lasers (AREA)
FI953079A 1992-12-22 1995-06-21 Förstärkning av DNA FI120741B (sv)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DK921539A DK153992D0 (da) 1992-12-22 1992-12-22 Metode
DK153992 1992-12-22
DK9300438 1993-12-22
PCT/DK1993/000438 WO1994014968A1 (en) 1992-12-22 1993-12-22 Dna amplification
CN94109005 1994-06-22
CN94109005A CN1063488C (zh) 1992-12-22 1994-06-22 Dna扩增

Publications (3)

Publication Number Publication Date
FI953079A FI953079A (sv) 1995-06-21
FI953079A0 true FI953079A0 (sv) 1995-06-21
FI120741B FI120741B (sv) 2010-02-15

Family

ID=37075831

Family Applications (1)

Application Number Title Priority Date Filing Date
FI953079A FI120741B (sv) 1992-12-22 1995-06-21 Förstärkning av DNA

Country Status (11)

Country Link
EP (1) EP0675959B1 (sv)
JP (1) JP3983798B2 (sv)
CN (1) CN1063488C (sv)
AT (1) ATE323167T1 (sv)
AU (1) AU679707B2 (sv)
CA (1) CA2152371C (sv)
DE (1) DE69334006T2 (sv)
DK (2) DK153992D0 (sv)
FI (1) FI120741B (sv)
NO (1) NO952496L (sv)
WO (1) WO1994014968A1 (sv)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU684275B2 (en) * 1993-03-15 1997-12-11 Cell Genesys, Inc. Method for defined deletions of DNA
EP0817856A1 (en) * 1995-03-22 1998-01-14 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
FR2762842B1 (fr) 1997-05-05 1999-07-23 Pasteur Institut Procede de detection de la presence de matieres biologiques d'origine bovine, et oligonucleotides pour sa mise en oeuvre
JP2002508669A (ja) * 1997-07-03 2002-03-19 ノボザイムス アクティーゼルスカブ 所望の遺伝子の複数コピー、及び選択マーカーではなく、スクリーニング可能マーカーを含むタンパク質産生細胞
CN100494363C (zh) 1997-12-22 2009-06-03 诺维信公司 糖氧化酶及其在焙烤中的用途
WO2002000907A1 (en) 2000-06-23 2002-01-03 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
DE60321901D1 (de) 2002-04-10 2008-08-14 Novozymes As Verbesserte bacillus-wirtszelle
EP1696035A3 (en) 2002-04-10 2008-04-02 Novozymes A/S Improved bacillus host cell
WO2005042750A1 (en) * 2003-10-31 2005-05-12 Novozymes A/S Method for stable gene-amplification in a bacterial host cell
US20070249016A1 (en) * 2003-11-25 2007-10-25 Japan Science And Technology Agency Method for Gene Amplification
US20080044853A1 (en) 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
CN101313064B (zh) 2005-11-24 2012-11-28 大学共同利用机关法人自然科学研究机构 基因扩增方法
EP2089516B1 (en) 2006-11-29 2011-11-16 Novozymes Inc. Methods of improving the introduction of dna into bacterial cells
WO2008140615A2 (en) 2006-12-21 2008-11-20 Novozymes, Inc. Modified messenger rna stabilizing sequences for expressing genes in bacterial cells
WO2010097436A1 (en) 2009-02-27 2010-09-02 Novozymes A/S Mutant cells having reduced expression of metallopeptidase, suitable for recombinant polypeptide production
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
EP2710132A1 (en) 2011-05-19 2014-03-26 Novozymes, Inc. Methods for enhancing the degradation of cellulosic material with chitin binding proteins
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
CN107287331B (zh) * 2017-08-01 2020-06-26 北京大学 一种高效检测蓝藻细胞周期的方法
CN111511908A (zh) 2017-11-10 2020-08-07 诺维信公司 温度敏感性cas9蛋白
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
CN113366113A (zh) 2019-01-30 2021-09-07 诺维信公司 同源折叠酶共表达
EP3935160A1 (en) 2019-03-08 2022-01-12 Novozymes A/S Means and methods for improving protease expression
US20220275354A1 (en) 2019-05-15 2022-09-01 Novozymes A/S TEMPERATURE-SENSITIVE RNA- Guided Endonuclease
CN114207125A (zh) 2019-06-25 2022-03-18 诺维信公司 通过抑制条件性必需基因进行反向选择
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2023247514A1 (en) 2022-06-22 2023-12-28 Novozymes A/S Recombinant mannanase expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (fr) * 1984-04-27 1986-08-22 Centre Nat Rech Scient Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
ES2151463T5 (es) * 1989-12-22 2012-10-29 Merck Serono Sa Constructos de ADN para la activación y la modificación de la expresión de genes endógenos

Also Published As

Publication number Publication date
ATE323167T1 (de) 2006-04-15
FI953079A (sv) 1995-06-21
WO1994014968A1 (en) 1994-07-07
AU679707B2 (en) 1997-07-10
CN1063488C (zh) 2001-03-21
JP3983798B2 (ja) 2007-09-26
NO952496L (no) 1995-08-21
NO952496D0 (no) 1995-06-21
JPH08504585A (ja) 1996-05-21
DE69334006T2 (de) 2006-11-23
AU5694494A (en) 1994-07-19
CA2152371A1 (en) 1994-07-07
EP0675959B1 (en) 2006-04-12
FI120741B (sv) 2010-02-15
DE69334006D1 (de) 2006-05-24
DK153992D0 (da) 1992-12-22
CN1114355A (zh) 1996-01-03
CA2152371C (en) 2008-12-02
DK0675959T3 (da) 2006-08-07
EP0675959A1 (en) 1995-10-11

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