ES2109194B1 - Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes. - Google Patents
Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.Info
- Publication number
- ES2109194B1 ES2109194B1 ES09600906A ES9600906A ES2109194B1 ES 2109194 B1 ES2109194 B1 ES 2109194B1 ES 09600906 A ES09600906 A ES 09600906A ES 9600906 A ES9600906 A ES 9600906A ES 2109194 B1 ES2109194 B1 ES 2109194B1
- Authority
- ES
- Spain
- Prior art keywords
- amino acid
- cells
- acid oxidase
- hospedant
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
- C12N9/0024—D-Amino acid oxidase (1.4.3.3)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
UN PROCEDIMIENTO PARA PRODUCIR LA ENZIMA D-AMINOACIDO OXIDASA DE RHODOTORULA GRACILIS EN CELULAS HOSPEDANTES. EL PROCEDIMIENTO DE EXPRESION DE LA ENZIMA COMPRENDE: AISLAR EL ADN COMPLEMENTARIO CORRESPONDIENTE AL ARN MENSAJERO DEL GEN QUE CODIFICA PARA LA D-AMINOACIDO OXIDASA DE CUALQUIER CEPA DE RHODOTORULA GRACILIS PRODUCTORA DE DICHA ENZIMA; FUSIONAR EL FRAGMENTO DE ADN QUE CODIFICA PARA LA D-AMINOACIDO OXIDASA DE RHODOTORULA GRACILIS A UNA SECUENCIA DE ADN QUE CONTENGA UN SITIO DE UNION AL RIBOSOMA Y UNA SECUENCIA PROMOTORA DE ALTA EFICACIA PARA LA EXPRESION DE GENES EN CELULAS HOSPEDANTES; INSERTAR EL FRAGMENTO DE ADN EN UN PLASMIDO DE APROPIADO PARA LA CELULA HOSPEDANTE; CULTIVAR LAS CELULAS HOSPEDANTES TRANSFORMADAS CON ESTE PLASMIDO; Y RECUPERAR LA ENZIMA.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09600906A ES2109194B1 (es) | 1996-04-22 | 1996-04-22 | Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes. |
US08/973,914 US6187574B1 (en) | 1996-04-22 | 1997-04-18 | Process for producing the enzyme D-amino acid oxidase of Rhodotorula gracilis in host cells |
AU23890/97A AU2389097A (en) | 1996-04-22 | 1997-04-18 | Process for producing the enzyme d-aminoacido oxydase of (rhodotorula gracilis) in host cells |
JP53776297A JP3476834B2 (ja) | 1996-04-22 | 1997-04-18 | 宿主細胞においてロドトルラ・グラシリスの酵素d−アミノ酸オキシダーゼを製造するための方法 |
EP97919405A EP0843015A1 (en) | 1996-04-22 | 1997-04-18 | PROCESS FOR PRODUCING THE ENZYME D-AMINOACIDO OXYDASE OF $i(RHODOTORULA GRACILIS) IN HOST CELLS |
PCT/ES1997/000099 WO1997040171A1 (es) | 1996-04-22 | 1997-04-18 | Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES09600906A ES2109194B1 (es) | 1996-04-22 | 1996-04-22 | Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2109194A1 ES2109194A1 (es) | 1998-01-01 |
ES2109194B1 true ES2109194B1 (es) | 1998-07-16 |
Family
ID=8294567
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES09600906A Expired - Fee Related ES2109194B1 (es) | 1996-04-22 | 1996-04-22 | Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes. |
Country Status (6)
Country | Link |
---|---|
US (1) | US6187574B1 (es) |
EP (1) | EP0843015A1 (es) |
JP (1) | JP3476834B2 (es) |
AU (1) | AU2389097A (es) |
ES (1) | ES2109194B1 (es) |
WO (1) | WO1997040171A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2131018B1 (es) | 1997-09-25 | 2000-04-01 | Antibioticos Sau | Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli. |
GB0201043D0 (en) * | 2002-01-17 | 2002-03-06 | Swetree Genomics Ab | Plants methods and means |
EP1375649B1 (en) * | 2002-06-19 | 2012-03-07 | Evonik Degussa GmbH | D-amino acid oxidase from Arthrobacter protophormiae |
DE102004008445A1 (de) * | 2004-02-19 | 2005-09-08 | Degussa Ag | Verfahren zur Herstellung von L-Aminosäuren aus D-Aminosäuren |
WO2007015511A1 (ja) | 2005-08-02 | 2007-02-08 | Kaneka Corporation | D-アミノ酸オキシダーゼ、およびl-アミノ酸、2-オキソ酸、又は環状イミンの製造方法。 |
EP2371946A1 (en) | 2010-03-30 | 2011-10-05 | Rijksuniversiteit Groningen | Improved methods and host cells for the production and use of D-amino acid oxidase (DAO). |
EP3277822B1 (en) * | 2015-04-03 | 2020-05-06 | Temasek Life Sciences Laboratory Limited | D-amino acid-inducible gene expression system for rhodosporidium and rhodotorula |
CN116376940A (zh) * | 2023-03-09 | 2023-07-04 | 浙江新安化工集团股份有限公司 | 一种表达d-氨基酸氧化酶的方法及其应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0364275B1 (en) * | 1988-10-13 | 1995-05-31 | Fujisawa Pharmaceutical Co., Ltd. | D-amino acid oxidase |
IT1252308B (it) * | 1990-12-21 | 1995-06-08 | Antibioticos Spa | Procedimento enzimatico per la produzione di acido 7- amminocefalosporanico e derivati |
IT1247964B (it) * | 1991-06-03 | 1995-01-05 | Ministero Dell Uni E Della | Processo per la preparazione enzimatica di derivati cefalosporanici |
CA2100987C (en) * | 1992-07-27 | 1999-06-15 | Kaoru Furuya | A transformant capable of producing d-amino acid oxidase |
IT1275909B1 (it) * | 1995-03-07 | 1997-10-24 | Mirella Pilone | Frammento di dna codificante d-amminoacido ossidasi |
US5877013A (en) * | 1997-07-31 | 1999-03-02 | Food Industry Research And Development Institute | Rhodosporidium D-amino acid oxidase |
-
1996
- 1996-04-22 ES ES09600906A patent/ES2109194B1/es not_active Expired - Fee Related
-
1997
- 1997-04-18 WO PCT/ES1997/000099 patent/WO1997040171A1/es not_active Application Discontinuation
- 1997-04-18 AU AU23890/97A patent/AU2389097A/en not_active Abandoned
- 1997-04-18 EP EP97919405A patent/EP0843015A1/en not_active Withdrawn
- 1997-04-18 JP JP53776297A patent/JP3476834B2/ja not_active Expired - Fee Related
- 1997-04-18 US US08/973,914 patent/US6187574B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US6187574B1 (en) | 2001-02-13 |
AU2389097A (en) | 1997-11-12 |
WO1997040171A1 (es) | 1997-10-30 |
EP0843015A1 (en) | 1998-05-20 |
JP3476834B2 (ja) | 2003-12-10 |
ES2109194A1 (es) | 1998-01-01 |
JPH11514241A (ja) | 1999-12-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EC2A | Search report published |
Date of ref document: 19980101 Kind code of ref document: A1 Effective date: 19980101 |
|
FD1A | Patent lapsed |
Effective date: 20070423 |