ES2131018B1 - Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli. - Google Patents

Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.

Info

Publication number
ES2131018B1
ES2131018B1 ES009702008A ES9702008A ES2131018B1 ES 2131018 B1 ES2131018 B1 ES 2131018B1 ES 009702008 A ES009702008 A ES 009702008A ES 9702008 A ES9702008 A ES 9702008A ES 2131018 B1 ES2131018 B1 ES 2131018B1
Authority
ES
Spain
Prior art keywords
escherichia coli
amino acid
enzyme
modified
carboxybutanamide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES009702008A
Other languages
English (en)
Other versions
ES2131018A1 (es
Inventor
Lopez Jose Luis Garcia
Rubio Estrella Cortes
Palacios Jorge Alonso
Duran Encarnacion Mellado
Garcia Bruno Diez
Seijas Jose Manuel Guisan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to ES009702008A priority Critical patent/ES2131018B1/es
Priority to ZA988754A priority patent/ZA988754B/xx
Priority to EEP199900205A priority patent/EE9900205A/xx
Priority to SK648-99A priority patent/SK64899A3/sk
Priority to EP98943898A priority patent/EP0969088A1/en
Priority to PCT/ES1998/000262 priority patent/WO1999015632A1/es
Priority to US09/308,683 priority patent/US6635458B2/en
Priority to IL12953398A priority patent/IL129533A0/xx
Priority to KR1019997004579A priority patent/KR20000069110A/ko
Priority to CN98801336A priority patent/CN1246147A/zh
Priority to CZ991823A priority patent/CZ182399A3/cs
Priority to HU0000797A priority patent/HUP0000797A2/hu
Priority to JP51859999A priority patent/JP2001506868A/ja
Priority to CA002272354A priority patent/CA2272354A1/en
Priority to AU91628/98A priority patent/AU9162898A/en
Priority to LT99-051A priority patent/LT4628B/lt
Priority to LVP-99-87A priority patent/LV12359B/en
Publication of ES2131018A1 publication Critical patent/ES2131018A1/es
Application granted granted Critical
Publication of ES2131018B1 publication Critical patent/ES2131018B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/06—Cephalosporin C; Derivatives thereof

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Un procedimiento enzimático para preparar ácido 7 b -(carboxibutanamido) cefalosporánico utilizando la enzima D-aminoácido oxidasa de trigonopsis variabilis modificada producida en Escherichia coli. El procedimiento de expresión de la enzima comprende: para la enzima D-aminoácido oxidasa; (II) eliminar el intrón que contiene dicho gen; (III) insertar el fragmento de ADN obtenido en un plásmido que sea capaz de replicarse en Escherichia coli (IV) fusionar en el extremo 5"'' de la región estructural del gen un ensamblador sintético que contiene una secuencia de nucleótidos que codifica para seis histidinas; (V) transformar una cepa de Escherichia coli con el plásmido recombinante resultante; (VI) cultivar las células de Escherichia coli transformadas y (VII) recuperar la enzima D-aminoácido oxidasa del cultivo de la operación anterior mediante cromatografía de afinidad.
ES009702008A 1997-09-25 1997-09-25 Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli. Expired - Fee Related ES2131018B1 (es)

Priority Applications (17)

Application Number Priority Date Filing Date Title
ES009702008A ES2131018B1 (es) 1997-09-25 1997-09-25 Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.
ZA988754A ZA988754B (en) 1997-09-25 1998-09-23 An enzymatic process for preparing 7beta- (4-carboxy-butanamido) cephalosporanic acid using the modified D-amino acid oxydase enzyme from trigonopsis variabilis produced in escherichia coli
HU0000797A HUP0000797A2 (en) 1997-09-25 1998-09-24 Enzymatic process for the preparation of cephalosporanic 7betha-(4-carboxybutanamide) acid by means of the modified enzyme d-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
EP98943898A EP0969088A1 (en) 1997-09-25 1998-09-24 ENZYMATIC PROCESS FOR THE PREPARATION OF CEPHALOSPORANIC 7$b(g)-(4-CARBOXYBUTANAMIDE) ACID BY MEANS OF THE MODIFIED ENZYME D-AMINOACID OXIDASE OF $i(TRIGONOPSIS VARIABILIS) PRODUCED IN $i(ESCHERICHIA COLI)
PCT/ES1998/000262 WO1999015632A1 (es) 1997-09-25 1998-09-24 UN PROCEDIMIENTO ENZIMATICO PARA PREPARAR ACIDO 7β-(4-CARBOXIBUTANAMIDO) CEFALOSPORANICO UTILIZANDO LA ENZIMA D-AMINOACIDO OXIDASA DE TRIGONOPSIS VARIABILIS MODIFICADA PRODUCIDA EN ESCHERICHIA COLI.
US09/308,683 US6635458B2 (en) 1997-09-25 1998-09-24 Enzymatic process for the preparation of cephalosporanic 7$B(G)-(4-Carboxybutanamide) acid by means of the modified enzyme D-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
IL12953398A IL129533A0 (en) 1997-09-25 1998-09-24 Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by means of the modified enzyme d-aminoacid oxidase of trgonopsis variabilis produced in escherichia coli
KR1019997004579A KR20000069110A (ko) 1997-09-25 1998-09-24 대장균에서 생성한 트리고노프시스 바리아빌리스의 변경 효소 ｄ
EEP199900205A EE9900205A (et) 1997-09-25 1998-09-24 Ensüümimeetod tsefalosporaan-7ß-(4-karboksübutaanamiid)happe valmistamiseks Escherichia coli's saadud Trigonopsis variabilis modifitseeritud D-aminohappe oksüdaasi abil
CZ991823A CZ182399A3 (cs) 1997-09-25 1998-09-24 Enzymatický způsob přípravy 7ß-(4-karboxybutanamido)cefalosporanové kyseliny pomocí modifikované oxidázy D-aminokyselin z Trigonopsis variabilis produkované v Escherichia coli
SK648-99A SK64899A3 (en) 1997-09-25 1998-09-24 Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by means of the modified enzyme d-aminoacid oxidase of i(trigonopsis variabilis) produced in i(escherichia coli)
JP51859999A JP2001506868A (ja) 1997-09-25 1998-09-24 大腸菌で生産したトリゴノプシス・バリアビリス由来のＤ―アミノ酸オキシダーゼ酵素修飾体を用いて、７β―（４―カルボキシブタンアミド）セファロスポラン酸を調製する酵素的方法
CA002272354A CA2272354A1 (en) 1997-09-25 1998-09-24 Enzymatic process for the preparation of cephalosporanic 7.beta.-(4-carboxybutanamide) acid by means of the modified enzyme d-aminoacid oxidase of trigonopsis variabilis produced in escherichia coli
AU91628/98A AU9162898A (en) 1997-09-25 1998-09-24 Enzymatic process for the preparation of cephalosporanic 7$b(g)-(4-carboxybu tanamide) acid by means of the modified enzyme d-aminoacid oxidase of (trigono psis variabilis) produced in (escherichia coli)
CN98801336A CN1246147A (zh) 1997-09-25 1998-09-24 由大肠杆菌产生的修饰的三角酵母D－氨基酸氧化酶制备头孢菌素烷基7β－(4－羧丁基酰胺)酸的方法
LT99-051A LT4628B (lt) 1997-09-25 1999-05-11 Cefalosporano 7beta-(4-karboksibutanamido)rūgšties fermentinis gavimo būdas, panaudojant modifikuotą trigonopsis variabilis d-aminorūgšties oksidazės fermentą, produkuotą escherichia coli
LVP-99-87A LV12359B (en) 1997-09-25 1999-05-25 FERMENTATIVE METHOD FOR DETERMINING 7ø- (4-CARBOXIBUTAMINE) CEFALOSPORATE ACID BY MODIFIED TRIGONOPSIS VARIABILIS FERMENTES D-AMINO ACQUISITION OXIDES PRODUCED IN ESCHERICHIA COLI

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009702008A ES2131018B1 (es) 1997-09-25 1997-09-25 Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.

Publications (2)

Publication Number Publication Date
ES2131018A1 ES2131018A1 (es) 1999-07-01
ES2131018B1 true ES2131018B1 (es) 2000-04-01

Family

ID=8300695

Family Applications (1)

Application Number Title Priority Date Filing Date
ES009702008A Expired - Fee Related ES2131018B1 (es) 1997-09-25 1997-09-25 Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.

Country Status (17)

Country Link
US (1) US6635458B2 (es)
EP (1) EP0969088A1 (es)
JP (1) JP2001506868A (es)
KR (1) KR20000069110A (es)
CN (1) CN1246147A (es)
AU (1) AU9162898A (es)
CA (1) CA2272354A1 (es)
CZ (1) CZ182399A3 (es)
EE (1) EE9900205A (es)
ES (1) ES2131018B1 (es)
HU (1) HUP0000797A2 (es)
IL (1) IL129533A0 (es)
LT (1) LT4628B (es)
LV (1) LV12359B (es)
SK (1) SK64899A3 (es)
WO (1) WO1999015632A1 (es)
ZA (1) ZA988754B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100342014C (zh) * 2004-04-08 2007-10-10 百瑞全球有限公司 重组d－氨基酸氧化酶
CN1912130B (zh) * 2005-08-08 2011-06-15 百瑞全球有限公司 一种用于制备7-氨基头孢霉烷酸的两步酶法
CN101016540B (zh) * 2006-02-10 2010-04-07 台湾尖端先进生技医药股份有限公司 C-端含有组氨酸标记的hoxb4重组蛋白质的制造方法及用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2268022B1 (es) * 1974-04-22 1977-06-24 Rhone Poulenc Ind
JPS62262994A (ja) * 1986-05-12 1987-11-16 Asahi Chem Ind Co Ltd Ｄ−アミノ酸オキシダ−ゼ遺伝子
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
CA2100987C (en) 1992-07-27 1999-06-15 Kaoru Furuya A transformant capable of producing d-amino acid oxidase
US5397653A (en) 1993-04-30 1995-03-14 Westinghouse Electric Corporation Filler wire composition and method of welding turbine component with filter wire composition and its product thereof
ES2109194B1 (es) 1996-04-22 1998-07-16 Antibioticos Sa Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.

Also Published As

Publication number Publication date
CZ182399A3 (cs) 1999-09-15
US20030119087A1 (en) 2003-06-26
LV12359B (en) 2000-03-20
LT4628B (lt) 2000-02-25
SK64899A3 (en) 2000-05-16
JP2001506868A (ja) 2001-05-29
EE9900205A (et) 1999-12-15
AU9162898A (en) 1999-04-12
US6635458B2 (en) 2003-10-21
ES2131018A1 (es) 1999-07-01
KR20000069110A (ko) 2000-11-25
IL129533A0 (en) 2000-02-29
HUP0000797A2 (en) 2000-07-28
WO1999015632A1 (es) 1999-04-01
LT99051A (en) 1999-10-25
LV12359A (lv) 1999-10-20
EP0969088A1 (en) 2000-01-05
ZA988754B (en) 1999-06-28
CN1246147A (zh) 2000-03-01
CA2272354A1 (en) 1999-04-01

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