CN101313064B - 基因扩增方法 - Google Patents
基因扩增方法 Download PDFInfo
- Publication number
- CN101313064B CN101313064B CN200680043921XA CN200680043921A CN101313064B CN 101313064 B CN101313064 B CN 101313064B CN 200680043921X A CN200680043921X A CN 200680043921XA CN 200680043921 A CN200680043921 A CN 200680043921A CN 101313064 B CN101313064 B CN 101313064B
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- China
- Prior art keywords
- double
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- sequence
- target sequence
- specific recombinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (12)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2005338119 | 2005-11-24 | ||
JP338119/2005 | 2005-11-24 | ||
PCT/JP2006/314168 WO2007060764A1 (ja) | 2005-11-24 | 2006-07-18 | 遺伝子増幅法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101313064A CN101313064A (zh) | 2008-11-26 |
CN101313064B true CN101313064B (zh) | 2012-11-28 |
Family
ID=38067002
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN200680043921XA Expired - Fee Related CN101313064B (zh) | 2005-11-24 | 2006-07-18 | 基因扩增方法 |
Country Status (5)
Country | Link |
---|---|
US (3) | US20090148895A1 (zh) |
EP (1) | EP1967585B1 (zh) |
JP (1) | JP4821012B2 (zh) |
CN (1) | CN101313064B (zh) |
WO (1) | WO2007060764A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105142397A (zh) * | 2013-03-13 | 2015-12-09 | 瑞泽恩制药公司 | 具有条件Acvr1突变型等位基因的啮齿类动物 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007060764A1 (ja) | 2005-11-24 | 2007-05-31 | Inter-University Research Institute Corporation National Institutes Of Natural Sciences | 遺伝子増幅法 |
US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6255082B1 (en) * | 1998-09-15 | 2001-07-03 | Yale University | Artificial long terminal repeat vectors |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK153992D0 (da) * | 1992-12-22 | 1992-12-22 | Novo Nordisk As | Metode |
AU2002219841A1 (en) * | 2000-11-16 | 2002-05-27 | Cornell Research Foundation Inc. | Vectors for conditional gene inactivation |
WO2005001087A2 (en) * | 2003-06-11 | 2005-01-06 | Regeneron Pharmaceuticals, Inc. | Methods of modifying genes in eukaryotic cells |
WO2005061703A1 (ja) * | 2003-11-25 | 2005-07-07 | Japan Science And Technology Agency | 遺伝子増幅法 |
WO2007060764A1 (ja) | 2005-11-24 | 2007-05-31 | Inter-University Research Institute Corporation National Institutes Of Natural Sciences | 遺伝子増幅法 |
-
2006
- 2006-07-18 WO PCT/JP2006/314168 patent/WO2007060764A1/ja active Application Filing
- 2006-07-18 US US12/085,476 patent/US20090148895A1/en not_active Abandoned
- 2006-07-18 EP EP06768267A patent/EP1967585B1/en not_active Not-in-force
- 2006-07-18 CN CN200680043921XA patent/CN101313064B/zh not_active Expired - Fee Related
- 2006-07-18 JP JP2007546357A patent/JP4821012B2/ja not_active Expired - Fee Related
-
2013
- 2013-05-09 US US13/890,533 patent/US9464307B2/en not_active Expired - Fee Related
-
2016
- 2016-09-09 US US15/261,274 patent/US20170037428A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6255082B1 (en) * | 1998-09-15 | 2001-07-03 | Yale University | Artificial long terminal repeat vectors |
Non-Patent Citations (3)
Title |
---|
A.J.Rattray等.A mechanism of palindromic gene amplification in Saccharomyces cerevisiae.《Genes Dev.》.2005,第19卷(第11期),1390-1399. * |
T Watanabe、T Horiuchi.A novel gene amplification system in yeast based on double rolling-circle replication.《The EMBO Journal》.2004,第24卷(第1期),190-198. * |
王立霞等.Cre重组酶结构与功能的研究进展.《生物工程学报》.2002,第18卷(第5期), * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105142397A (zh) * | 2013-03-13 | 2015-12-09 | 瑞泽恩制药公司 | 具有条件Acvr1突变型等位基因的啮齿类动物 |
CN105142397B (zh) * | 2013-03-13 | 2018-02-06 | 瑞泽恩制药公司 | DNA构建体和制备具有条件突变型Acvr1等位基因的小鼠的方法 |
US9999207B2 (en) | 2013-03-13 | 2018-06-19 | Regeneron Pharmaceuticals, Inc. | Nucleic acid construct for making a genetically modified rodent with an inducible ACVR1 mutation that causes ectopic bone formation |
Also Published As
Publication number | Publication date |
---|---|
EP1967585A4 (en) | 2010-07-07 |
JP4821012B2 (ja) | 2011-11-24 |
WO2007060764A1 (ja) | 2007-05-31 |
JPWO2007060764A1 (ja) | 2009-05-07 |
US20090148895A1 (en) | 2009-06-11 |
EP1967585A1 (en) | 2008-09-10 |
US20130266988A1 (en) | 2013-10-10 |
EP1967585B1 (en) | 2011-12-28 |
US9464307B2 (en) | 2016-10-11 |
CN101313064A (zh) | 2008-11-26 |
US20170037428A1 (en) | 2017-02-09 |
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Content | Transformation By Infection |
Legal Events
Date | Code | Title | Description |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: TAKAAKI WATANABE Effective date: 20150713 Owner name: TAKASHI HORIUCHI Free format text: FORMER OWNER: INTER-UNIVERSITY RESEARCH INSTITUTE CORPORATION NATIONAL INSTITUTES OF NATURAL SCIENCES Effective date: 20150713 Owner name: GENODIVE PHARMA INC. Free format text: FORMER OWNER: TAKASHI HORIUCHI Effective date: 20150713 Owner name: TAKAAKI WATANABE Effective date: 20150713 |
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C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20150713 Address after: Yokohama City, Kanagawa Prefecture, Japan Patentee after: GENODIVE PHARMA Inc. Address before: The city of Okazaki, Aichi Prefecture Patentee before: Horiuchi Takashi Patentee before: Watake Takaaki Effective date of registration: 20150713 Address after: The city of Okazaki, Aichi Prefecture Patentee after: Horiuchi Takashi Patentee after: Watake Takaaki Address before: Tokyo, Japan Patentee before: INTER University RESEARCH INSTITUTE CORPORATION NATIONAL INSTITUTES OF NATURAL SCIENCES |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121128 Termination date: 20190718 |