ATE68205T1 - Vektor und verfahren zur erreichung einer hohen expression in zellen. - Google Patents

Vektor und verfahren zur erreichung einer hohen expression in zellen.

Info

Publication number
ATE68205T1
ATE68205T1 AT87300658T AT87300658T ATE68205T1 AT E68205 T1 ATE68205 T1 AT E68205T1 AT 87300658 T AT87300658 T AT 87300658T AT 87300658 T AT87300658 T AT 87300658T AT E68205 T1 ATE68205 T1 AT E68205T1
Authority
AT
Austria
Prior art keywords
enhancer element
dna
vector
gene encoding
region
Prior art date
Application number
AT87300658T
Other languages
English (en)
Inventor
Stephen D Gillies
Original Assignee
Abbott Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25274910&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE68205(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Abbott Biotech Inc filed Critical Abbott Biotech Inc
Application granted granted Critical
Publication of ATE68205T1 publication Critical patent/ATE68205T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Generation (AREA)
  • Enzymes And Modification Thereof (AREA)
AT87300658T 1986-03-07 1987-01-27 Vektor und verfahren zur erreichung einer hohen expression in zellen. ATE68205T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83759586A 1986-03-07 1986-03-07
EP87300658A EP0237157B1 (de) 1986-03-07 1987-01-27 Vektor und Verfahren zur Erreichung einer hohen Expression in Zellen

Publications (1)

Publication Number Publication Date
ATE68205T1 true ATE68205T1 (de) 1991-10-15

Family

ID=25274910

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87300658T ATE68205T1 (de) 1986-03-07 1987-01-27 Vektor und verfahren zur erreichung einer hohen expression in zellen.

Country Status (9)

Country Link
US (1) US5665578A (de)
EP (1) EP0237157B1 (de)
JP (1) JP2535000B2 (de)
AT (1) ATE68205T1 (de)
AU (1) AU582288B2 (de)
CA (1) CA1304026C (de)
DE (1) DE3773512D1 (de)
DK (1) DK47887A (de)
NO (1) NO870948L (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0255320A3 (de) * 1986-07-28 1989-09-06 Genzyme Corporation Herstellung von Proteinen in Myelomazellen
CA1297435C (en) * 1986-09-12 1992-03-17 Stephen D. Gillies Messenger rna stabilization in animal cells
GB8629153D0 (en) * 1986-12-05 1987-01-14 Ciba Geigy Ag Hybrid proteins
FR2629469B1 (fr) * 1988-03-31 1990-12-21 Pasteur Institut Retrovirus recombinant defectif, son application a l'integration de sequences codantes pour des proteines determinees dans le genome de cultures cellulaires infectables par le retrovirus sauvage correspondant et adns recombinants pour la production de ce retrovirus recombinant
AU644352B2 (en) * 1988-12-08 1993-12-09 Damon Biotech Inc. Expression induction method employing mutant dhfr gene
US5196320A (en) * 1989-09-20 1993-03-23 Abbott Biotech, Inc. Method of producing engineered binding proteins
JP2002510502A (ja) * 1998-04-03 2002-04-09 インビトロジェン コーポレイション 発現可能な遺伝子配列のライブラリーの産生方法
US20020034772A1 (en) * 1999-06-29 2002-03-21 Orlow Seth J. Methods and compositions that affect melanogenesis
CA2443266A1 (en) * 2001-02-13 2002-08-22 University Of Florida A bi-directional dual promoter complex with enhanced promoter activity for transgene expression in eukaryotes
JPWO2004070030A1 (ja) * 2003-02-03 2006-05-25 株式会社イムノ・ジャパン 動物細胞用高発現ベクター
IL315208A (en) 2022-02-24 2024-10-01 Sinomab Bioscience Ltd Bispecific binding proteins against alarmins and uses thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2440957A1 (fr) * 1978-11-13 1980-06-06 Pasteur Institut Vecteurs appropries a l'insertion dans leurs genomes de fragments d'adn etrangers, selon l'une quelconque des phases de traduction possibles, et moyens pour leur fabrication
FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
GB2052516B (en) * 1979-06-01 1983-06-29 Searle & Co Plasmid vectors production and use thereof
US4418149A (en) * 1980-01-10 1983-11-29 President And Fellows Of Harvard College Fused hybrid gene
US4399216A (en) * 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
AU546577B2 (en) * 1980-02-25 1985-09-05 Trustees Of Columbia University, The Use of eucaryotic promoter sequences in the production of proteinaceous materials
JPS57501112A (de) * 1980-07-08 1982-07-01
DK489481A (da) * 1980-11-10 1982-05-11 Searle & Co Plasmidvektor og frmgangsmaade til fremstilling deraf
US4374927A (en) * 1981-02-24 1983-02-22 The Board Of Trustees Of The Leland Stanford Jr. University Extrachromosomal regulation of expression
JPS57181098A (en) * 1981-04-30 1982-11-08 Japan Found Cancer Novel recombinant dna
IL65775A (en) * 1981-05-18 1985-05-31 Genentech Inc Microbial hybrid promotors and plasmids and e.coli comprising them
IL66065A (en) * 1981-06-22 1989-06-30 Lilly Co Eli Recombinant dna cloning vectors and the e.coli transformants thereof
US4405712A (en) * 1981-07-01 1983-09-20 The United States Of America As Represented By The Department Of Health And Human Services LTR-Vectors
CA1207253A (en) * 1981-08-17 1986-07-08 Lee D. Simon T4 dna fragment as a stabilizer for proteins expressed by cloned dna
JPS58501406A (ja) * 1981-08-21 1983-08-25 ユニヴア−シテイ−　パテンツ，インコ−ポレ−テツド エシエリヒア・コリ−における蛋白合成発現の高揚
ZW18282A1 (en) * 1981-08-31 1983-03-23 Genentech Inc Preparation of polypeptides in vertebrate cell culture
DE3279270D1 (en) * 1981-09-02 1989-01-12 Biogen Nv Amplified expression of dna sequences
JPS58146281A (ja) * 1981-10-19 1983-08-31 Suntory Ltd 酵母細胞の形質転換法
EP0146743B1 (de) * 1983-11-08 1992-07-29 Teijin Limited Genfragmente, abgeleitet vom menschlichen Immunoglobulin-Gen
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
DOP1985004337A (es) * 1984-03-06 1990-09-17 Lilly Co Eli Vectores y metodo para expresion genetica
JPH0789930B2 (ja) * 1984-03-08 1995-10-04 大正製薬株式会社 ウイルス性転写促進配列
US4663281A (en) * 1984-03-22 1987-05-05 Mass Institute Of Technology Enhanced production of proteinaceous materials in eucaryotic cells
US4931373A (en) * 1984-05-25 1990-06-05 Zymogenetics, Inc. Stable DNA constructs for expression of α-1 antitrypsin
DE3431140A1 (de) * 1984-08-24 1986-03-06 Behringwerke Ag, 3550 Marburg Enhancer fuer eukaryotische expressionssysteme
DE3668186D1 (de) * 1985-04-01 1990-02-15 Celltech Ltd Transformierte myeloma-zell-linie und dieselbe verwendendes verfahren zur expression eines gens, das ein eukaryontisches polypeptid kodiert.
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
US5001230A (en) * 1988-02-18 1991-03-19 Schering Corporation T cell activation markers

Also Published As

Publication number Publication date
JP2535000B2 (ja) 1996-09-18
NO870948D0 (no) 1987-03-06
NO870948L (no) 1987-09-08
DK47887A (da) 1987-09-08
EP0237157A2 (de) 1987-09-16
US5665578A (en) 1997-09-09
DK47887D0 (da) 1987-01-29
CA1304026C (en) 1992-06-23
EP0237157B1 (de) 1991-10-09
AU582288B2 (en) 1989-03-16
AU6568386A (en) 1987-10-15
DE3773512D1 (de) 1991-11-14
JPS62210990A (ja) 1987-09-17
EP0237157A3 (en) 1989-04-05

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UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee