PL2963709T3 - Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych - Google Patents

Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych

Info

Publication number
PL2963709T3
PL2963709T3 PL15179339T PL15179339T PL2963709T3 PL 2963709 T3 PL2963709 T3 PL 2963709T3 PL 15179339 T PL15179339 T PL 15179339T PL 15179339 T PL15179339 T PL 15179339T PL 2963709 T3 PL2963709 T3 PL 2963709T3
Authority
PL
Poland
Prior art keywords
methods
nucleic acids
transposon end
modifying nucleic
end compositions
Prior art date
Application number
PL15179339T
Other languages
English (en)
Inventor
Jerome Jendrisak
Gary Dahl
Haiying Li Grunenwald
Nicholas Caruccio
Original Assignee
Epicentre Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42119714&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2963709(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Epicentre Technologies Corporation filed Critical Epicentre Technologies Corporation
Publication of PL2963709T3 publication Critical patent/PL2963709T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL15179339T 2008-10-24 2009-10-24 Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych PL2963709T3 (pl)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US10832608P 2008-10-24 2008-10-24
US10832108P 2008-10-24 2008-10-24
US10832908P 2008-10-24 2008-10-24
US15286809P 2009-02-16 2009-02-16
US15543109P 2009-02-25 2009-02-25
US18453009P 2009-06-05 2009-06-05
EP15179339.5A EP2963709B1 (en) 2008-10-24 2009-10-24 Transposon end compositions and methods for modifying nucleic acids

Publications (1)

Publication Number Publication Date
PL2963709T3 true PL2963709T3 (pl) 2017-11-30

Family

ID=42119714

Family Applications (1)

Application Number Title Priority Date Filing Date
PL15179339T PL2963709T3 (pl) 2008-10-24 2009-10-24 Kompozycje końców transpozonu i sposoby modyfikacji kwasów nukleinowych

Country Status (13)

Country Link
EP (6) EP2963709B1 (pl)
JP (1) JP5794572B2 (pl)
CN (2) CN102264914B (pl)
CA (1) CA2750054C (pl)
DK (4) DK2787565T3 (pl)
ES (5) ES2637843T3 (pl)
HK (1) HK1249765B (pl)
HU (1) HUE027961T2 (pl)
LT (1) LT2963709T (pl)
PL (1) PL2963709T3 (pl)
PT (2) PT2963709T (pl)
SI (1) SI2963709T1 (pl)
WO (1) WO2010048605A1 (pl)

Families Citing this family (191)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2240606B1 (en) 2008-01-14 2016-10-12 Applied Biosystems, LLC Compositions, methods, and kits for detecting ribonucleic acid
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
PL2539450T3 (pl) * 2010-02-25 2016-08-31 Advanced Liquid Logic Inc Sposób wytwarzania bibliotek kwasu nukleinowego
EP2545183B1 (en) * 2010-03-10 2017-04-19 Ibis Biosciences, Inc. Production of single-stranded circular nucleic acid
EP2635679B1 (en) * 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
CN103732744A (zh) * 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
WO2013020089A2 (en) * 2011-08-04 2013-02-07 Sage Science, Inc. Systems and methods for processing fluids
US10689643B2 (en) * 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
EP2783001B1 (en) * 2011-11-22 2018-01-03 Active Motif Multiplex isolation of protein-associated nucleic acids
NO2694769T3 (pl) 2012-03-06 2018-03-03
CN102703426A (zh) * 2012-05-21 2012-10-03 吴江汇杰生物科技有限公司 构建核酸库的方法、试剂及试剂盒
WO2013181265A1 (en) * 2012-05-29 2013-12-05 The Johns Hopkins University Eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
CN102703432A (zh) * 2012-07-11 2012-10-03 烟台博诺生物科技有限公司 构建核酸库的方法、试剂及试剂盒
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2885418A4 (en) 2012-08-14 2016-03-02 10X Genomics Inc MICROCAPSE COMPOSITIONS AND METHOD THEREFOR
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
EP2906935B1 (en) 2012-10-12 2018-04-04 Sage Science, Inc. Side-eluting molecular fractionator
US9868946B2 (en) 2012-11-05 2018-01-16 Icahn School Of Medicine At Mount Sinai Method of isolating pure mitochondrial DNA
CN103849672B (zh) * 2012-12-06 2017-06-06 中国科学院上海生命科学研究院 一组糖基转移酶及其应用
WO2014093330A1 (en) * 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
KR20230003659A (ko) 2013-02-08 2023-01-06 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
EP3919617A1 (en) 2013-03-13 2021-12-08 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014171898A2 (en) 2013-04-17 2014-10-23 Agency For Science, Technology And Research Method for generating extended sequence reads
DK2999784T4 (en) * 2013-05-22 2023-02-20 Active Motif Inc Målrettet transposition til anvendelse i epigenetiske studier
EP3828285B1 (en) 2013-05-23 2023-12-13 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
US10655167B2 (en) 2013-07-26 2020-05-19 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9217167B2 (en) * 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
EP3066114B1 (en) * 2013-11-07 2019-11-13 Agilent Technologies, Inc. Plurality of transposase adapters for dna manipulations
US10597715B2 (en) * 2013-12-05 2020-03-24 Centrillion Technology Holdings Methods for sequencing nucleic acids
CN103710342A (zh) * 2013-12-18 2014-04-09 浙江农林大学 人工诱导的mle转座子试剂盒及其应用
CN106414765A (zh) 2013-12-20 2017-02-15 Illumina公司 在片段化的基因组dna样品中保留基因组连接信息
CN105899683A (zh) * 2014-01-14 2016-08-24 凯杰有限公司 带标签dna片段的产生
CN106103713B (zh) * 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
US11136576B2 (en) 2014-02-03 2021-10-05 Thermo Fisher Scientific Baltics Uab Method for controlled DNA fragmentation
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP4219010A1 (en) 2014-04-10 2023-08-02 10X Genomics, Inc. Methods for encapsulating and partitioning reagents
WO2015157611A2 (en) * 2014-04-11 2015-10-15 The Johns Hopkins University Improvements to eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
EP3845640A3 (en) 2014-04-15 2021-09-01 Illumina, Inc. Modified tranposases for improved insertion sequence bias and increased dna input tolerence
WO2015168161A2 (en) 2014-04-29 2015-11-05 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
EP3146046B1 (en) 2014-05-23 2020-03-11 Digenomix Corporation Haploidome determination by digitized transposons
EP3715468A1 (en) 2014-06-13 2020-09-30 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2015200869A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
ES2713153T3 (es) 2014-06-30 2019-05-20 Illumina Inc Métodos y composiciones que utilizan la transposición unilateral
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
JP6483249B2 (ja) * 2014-09-12 2019-03-13 エムジーアイ テック カンパニー リミテッドＭＧＩ Ｔｅｃｈ Ｃｏ．， Ｌｔｄ． 単離されたオリゴヌクレオチドおよび核酸の配列決定におけるその使用
CN106715713B (zh) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 试剂盒及其在核酸测序中的用途
CN105525357B (zh) * 2014-09-30 2018-08-21 深圳华大基因股份有限公司 一种测序文库的构建方法及试剂盒和应用
WO2016061098A1 (en) * 2014-10-14 2016-04-21 Covaris, Inc. Acoustic energy mediation of genetic fragmentation
US20170292153A1 (en) * 2014-10-14 2017-10-12 Bgi Shenzhen Co., Limited Method for breaking nucleic acid and adding adaptor by means of transposase, and reagent
CN107075560B (zh) * 2014-10-14 2021-01-26 深圳华大智造科技股份有限公司 一种核酸的转座酶打断一站式处理方法及试剂
GB201418159D0 (en) * 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN107109487B (zh) 2014-10-15 2022-03-04 赛琪科学股份有限公司 自动处理核酸和电泳样品制备的装置、方法和系统
LT3207134T (lt) * 2014-10-17 2019-09-10 Illumina Cambridge Limited Sukibimą išsauganti transpozicija
JP6808617B2 (ja) * 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
JP2017532042A (ja) 2014-10-29 2017-11-02 １０エックス ゲノミクス，インコーポレイテッド 標的化核酸配列決定のための方法及び組成物
FI3628732T3 (fi) 2014-11-05 2023-05-31 Illumina Inc Transposaasikoostumuksia insertiovääristymän vähentämiseksi
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107109495B (zh) 2014-11-11 2021-12-24 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
EP3244992B1 (en) 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
CN105985945B (zh) * 2015-01-30 2019-04-23 深圳华大智造科技有限公司 mRNA片段化方法及基于其构建测序文库的方法
CA2975958A1 (en) 2015-02-24 2016-09-01 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016169431A1 (zh) * 2015-04-20 2016-10-27 深圳华大基因研究院 一种长片段dna文库构建方法
WO2016189331A1 (en) 2015-05-28 2016-12-01 Illumina Cambridge Limited Surface-based tagmentation
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
WO2017004083A1 (en) * 2015-06-29 2017-01-05 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
US10364457B2 (en) 2015-08-07 2019-07-30 Massachusetts Institute Of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
ES2988429T3 (es) 2015-08-28 2024-11-20 Illumina Inc Análisis de secuencia de ácidos nucleicos de células individuales
WO2017087979A1 (en) 2015-11-20 2017-05-26 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
PT3882357T (pt) 2015-12-04 2022-09-05 10X Genomics Inc Métodos e composições para análise de ácidos nucleicos
CN105524920B (zh) * 2016-01-21 2018-10-02 深圳华大基因研究院 用于Ion Proton测序平台的TN5建库的引物组、试剂盒和建库方法
WO2017197027A1 (en) 2016-05-11 2017-11-16 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
US10240196B2 (en) * 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US11414680B2 (en) 2016-08-30 2022-08-16 Kyushu University National University Corporation Method for inserting desired DNA fragment into site located adjacent to binding domain of DNA-binding protein
US11021738B2 (en) * 2016-12-19 2021-06-01 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented DNA
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN112986589B (zh) * 2017-01-05 2023-01-24 深圳迈瑞生物医疗电子股份有限公司 网织红细胞模拟粒子、血小板模拟粒子制备方法及质控物
CN106801044B (zh) * 2017-01-09 2021-03-23 北京全式金生物技术有限公司 一种环状转座子复合物及其应用
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
SG11201810639RA (en) 2017-02-21 2018-12-28 Illumina Inc Tagmentation using immobilized transposomes with linkers
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018187779A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
WO2018217625A1 (en) * 2017-05-23 2018-11-29 Bio-Rad Laboratories, Inc. Molecular barcoding
CA3064709A1 (en) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
CN107586835B (zh) * 2017-10-19 2020-11-03 东南大学 一种基于单链接头的下一代测序文库的构建方法及其应用
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
WO2019089959A1 (en) * 2017-11-02 2019-05-09 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
WO2019099751A1 (en) 2017-11-15 2019-05-23 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019152108A1 (en) 2018-02-05 2019-08-08 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
WO2019156957A1 (en) 2018-02-06 2019-08-15 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
CN112513268B (zh) * 2018-02-08 2025-01-21 通用测序技术公司 用于核酸测序的追踪核酸片段来源的方法和组合物
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
CN110219054B (zh) * 2018-03-04 2020-10-02 清华大学 一种核酸测序文库及其构建方法
WO2019195166A1 (en) 2018-04-06 2019-10-10 10X Genomics, Inc. Systems and methods for quality control in single cell processing
EP3781711B1 (en) 2018-04-19 2024-11-20 Massachusetts Institute Of Technology Single-stranded break detection in double-stranded dna
JP7407128B2 (ja) * 2018-05-03 2023-12-28 ベクトン・ディキンソン・アンド・カンパニー ハイスループットマルチオミクスサンプル解析
EP4582554A3 (en) 2018-05-08 2025-07-30 MGI Tech Co., Ltd. Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN113249796B (zh) * 2018-06-20 2024-08-27 深圳海普洛斯医学检验实验室 对dna片段进行标记的单分子标签
DK3821011T5 (da) * 2018-07-11 2024-08-19 Cygnus Biosciences Beijing Co Ltd Transposom-aktiveret dna/rna-sekventering (ted rna-seq)
WO2020013833A1 (en) 2018-07-13 2020-01-16 Massachusetts Institute Of Technology Dimethylacrylamide (dmaa) hydrogel for expansion microscopy (exm)
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
CN112359090A (zh) 2018-08-07 2021-02-12 深圳市真迈生物科技有限公司 一种核酸片段的连接方法、测序文库的构建方法及其应用
WO2020043803A1 (en) 2018-08-28 2020-03-05 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
CN112739829A (zh) * 2018-09-27 2021-04-30 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
US12104281B2 (en) 2018-11-30 2024-10-01 Illumina, Inc. Analysis of multiple analytes using a single assay
SG11202102424QA (en) 2018-12-17 2021-04-29 Illumina Inc Methods and means for preparing a library for sequencing
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
BR112021006038A2 (pt) 2019-01-11 2021-10-26 Illumina Cambridge Limited Complexos de stranspossomas ligados à superfície do complexo
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
US11584953B2 (en) 2019-02-12 2023-02-21 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
US12405193B2 (en) 2019-02-22 2025-09-02 Massachusetts Institute Of Technology Iterative direct expansion microscopy
WO2020210802A1 (en) * 2019-04-11 2020-10-15 University Of Virginia Patent Foundation Tagmentation to open up circles of dna and detect extrachromosomal circles of dna for diagnosis
CN110257479A (zh) * 2019-06-25 2019-09-20 北京全式金生物技术有限公司 一种快速构建rna 3’端基因表达文库的方法
AU2020312787A1 (en) 2019-07-12 2021-06-17 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
WO2021035180A1 (en) * 2019-08-22 2021-02-25 New England Biolabs, Inc. Cleavage of single stranded dna having a modified nucleotide
WO2021048444A1 (en) 2019-09-12 2021-03-18 Thermo Fisher Scientific Baltics Uab Recombinant transposon ends
CN110564705A (zh) * 2019-09-30 2019-12-13 苏州璞瑞卓越生物科技有限公司 一种用于转座酶片段化的试剂及其应用
WO2021077415A1 (en) 2019-10-25 2021-04-29 Peking University Methylation detection and analysis of mammalian dna
US12265004B2 (en) 2019-11-05 2025-04-01 Massachusetts Institute Of Technology Membrane probes for expansion microscopy
AU2020396889A1 (en) 2019-12-04 2021-09-30 Illumina, Inc. Preparation of DNA sequencing libraries for detection of DNA pathogens in plasma
WO2021113505A1 (en) 2019-12-05 2021-06-10 Massachusetts Institute Of Technology Method for preparing a specimen for expansion microscopy
EP4097228B1 (en) 2020-01-29 2024-08-14 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
WO2021252617A1 (en) * 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
KR20230020977A (ko) * 2020-06-09 2023-02-13 일루미나, 인코포레이티드 시퀀싱 라이브러리의 생성을 증가시키는 방법
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116194589A (zh) 2020-07-31 2023-05-30 贝克顿迪金森公司 用于转座酶可及染色质的单细胞测定
WO2022132206A1 (en) 2020-12-15 2022-06-23 Becton, Dickinson And Company Single cell secretome analysis
CN114015755B (zh) * 2020-12-31 2024-03-01 中国科学院北京基因组研究所(国家生物信息中心) 用于标记核酸分子的方法和试剂盒
EP4294933A4 (en) * 2021-02-19 2025-10-15 Nuclear Rna Networks Inc COMPOSITIONS AND METHODS FOR MODULATING GENE TRANSCRIPTION NETWORKS BASED ON SHARED HIGH-IDENTITY TRANSPOSABLE ELEMENT SEQUENCES AND NON-PROCESSIVE PROMOTER TRANSCRIPTS AND PROMOTER-PROXIMAL TRANSCRIPTS
AU2022252197A1 (en) 2021-03-29 2023-09-21 Illumina, Inc. Improved methods of library preparation
CA3223731A1 (en) 2021-08-11 2023-02-16 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
WO2023175021A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods of preparing loop fork libraries
EP4590854A1 (en) 2022-09-19 2025-07-30 Illumina, Inc. Deformable polymers comprising immobilised primers
CN115386622B (zh) * 2022-10-26 2023-10-27 北京寻因生物科技有限公司 一种转录组文库的建库方法及其应用
WO2024256580A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025062002A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Concurrent sequencing using nick translation
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
CN118043478B (zh) 2023-10-20 2025-03-14 北京昌平实验室 用于可靠的无创胚胎植入前基因检测的方法
WO2025083338A1 (en) * 2023-10-20 2025-04-24 Domus Biotechnologies Oy An in vitro method for fragmenting polynucleotides for next generation sequencing
WO2025244841A1 (en) 2024-05-23 2025-11-27 Illumina, Inc. Methods and systems for quantifying targeted enrichment efficiency

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
KR100189229B1 (ko) 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
WO1997035026A1 (en) 1996-03-18 1997-09-25 Molecular Biology Resources, Inc. Target nucleic acid sequence amplification
US5773733A (en) 1996-04-12 1998-06-30 National Science Council Alumina-aluminum nitride-nickel composites
US5925545A (en) * 1996-09-09 1999-07-20 Wisconsin Alumni Research Foundation System for in vitro transposition
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
FI103809B (fi) * 1997-07-14 1999-09-30 Finnzymes Oy In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
DE69937223T3 (de) 1998-11-09 2011-05-05 Eiken Kagaku K.K. Verfahren zur synthese von nukleinsäuren
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6155972A (en) 1999-02-02 2000-12-05 Acorn Cardiovascular, Inc. Cardiac constraint jacket construction
CN1227357C (zh) 1999-03-19 2005-11-16 宝生物工程株式会社 用于扩增核酸序列的方法
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
CN102586228A (zh) 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
CN1854309A (zh) 2000-08-23 2006-11-01 宝生物工程株式会社 核酸扩增方法
EP1339863A2 (en) * 2000-12-05 2003-09-03 Wisconsin Alumni Research Foundation Double transposition methods for manipulating nucleic acids
FR2822164B1 (fr) 2001-03-19 2004-06-18 Centre Nat Rech Scient Polypeptides derives des arn polymerases, et leurs utilisations
FI20020746A7 (fi) * 2002-04-18 2003-10-19 Finnzymes Oy Menetelmä ja materiaalit polypeptidien deleetiojohdannaisten tuottamiseksi
US7108976B2 (en) * 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
AU2003260946A1 (en) 2002-09-20 2004-04-08 Prokaria Ehf. Thermostable rna ligase from thermus phage
US20070059692A1 (en) * 2002-10-28 2007-03-15 Xiaolian Gao Array oligomer synthesis and use
AU2003294440A1 (en) 2002-11-21 2004-06-18 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
AU2004209426B2 (en) 2003-01-29 2008-04-17 454 Life Sciences Corporation Method for preparing single-stranded DNA libraries
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
AU2006320275B2 (en) * 2005-12-02 2012-06-07 Synthetic Genomics, Inc. Synthesis of error-minimized nucleic acid molecules
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
EP2495337A1 (en) 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays

Also Published As

Publication number Publication date
HK1197498A1 (en) 2015-01-16
EP2787565B1 (en) 2015-09-16
CA2750054C (en) 2018-05-29
HK1249765B (en) 2020-04-17
ES2403756T3 (es) 2013-05-21
ES2637843T3 (es) 2017-10-17
EP3272879B1 (en) 2019-08-07
EP2508529B1 (en) 2013-08-28
EP2787565A1 (en) 2014-10-08
WO2010048605A1 (en) 2010-04-29
EP2376517A1 (en) 2011-10-19
EP2963709B1 (en) 2017-05-24
PT2787565E (pt) 2015-11-17
LT2963709T (lt) 2017-09-25
ES2550237T3 (es) 2015-11-05
SI2963709T1 (sl) 2017-10-30
HUE027961T2 (en) 2016-11-28
EP2664678B1 (en) 2014-10-08
JP5794572B2 (ja) 2015-10-14
EP2376517B1 (en) 2013-01-16
DK2376517T3 (da) 2013-02-11
CN105018548A (zh) 2015-11-04
CN102264914A (zh) 2011-11-30
HK1169659A1 (en) 2013-02-01
HK1163107A1 (en) 2012-09-07
PT2963709T (pt) 2017-08-21
EP2664678A1 (en) 2013-11-20
CA2750054A1 (en) 2010-04-29
DK2963709T3 (en) 2017-09-11
JP2012506704A (ja) 2012-03-22
EP3272879A1 (en) 2018-01-24
EP2508529A1 (en) 2012-10-10
HK1217032A1 (zh) 2016-12-16
CN102264914B (zh) 2015-08-12
ES2515065T3 (es) 2014-10-29
CN105018548B (zh) 2019-03-12
HK1219806A1 (en) 2017-04-13
ES2743029T3 (es) 2020-02-18
DK3272879T3 (da) 2019-08-26
ES2743029T8 (es) 2020-02-27
EP2963709A1 (en) 2016-01-06
DK2787565T3 (en) 2015-10-12
EP2376517A4 (en) 2012-06-27

Similar Documents

Publication Publication Date Title
SI2963709T1 (sl) Transposonski sestavki in postopki za spreminjanje nukleinskih kislin
GB2471044B (en) Methods and compositions for base calling nucleic acids
GB0815872D0 (en) Novel method and compositions
IL210153A0 (en) Nutrigenomics methods and compositions
IL202184A0 (en) Methods and compositions for increased transgene expression
IL212822A0 (en) Anti-cxcr1 compositions and methods
GB0819530D0 (en) Methods and compositions
IL209829A0 (en) Compositions and methods for enhanced somatostatin immunogenicity
IL210559A0 (en) Novel compositions and methods
ZA201102195B (en) Methods and compositions for isolating nucleic acid
IL210588A0 (en) Novel compositions and methods
EP2357164A4 (en) PROCESS FOR THE PRODUCTION OF A -SUBSTITUTED ESTER
GB0817585D0 (en) Novel compositions and methods
GB0811250D0 (en) Methods and compositions
GB0801122D0 (en) Vaccine composition
EP2373817A4 (en) METHOD AND COMPOSITIONS FOR HYBRIDIZING NUCLEIC ACIDS
EP2332902A4 (en) PROCESS FOR PRODUCING A -TRIFLUOROMETHYL-ESTER, -INSATURATED
GB0807102D0 (en) Process and composition
GB0807579D0 (en) Vaccine composition and method
GB0802079D0 (en) Method and compositions
PL2293760T3 (pl) Kompozycje do nadawania superhydrobofobowości
GB0806334D0 (en) Method and composition
GB0816946D0 (en) Composition and method
GB0817553D0 (en) Composition and method
GB0804008D0 (en) Improved method and compositions for joining fishing lines