SG11202007686VA - Methods characterizing multiple analytes from individual cells or cell populations - Google Patents

Methods characterizing multiple analytes from individual cells or cell populations

Info

Publication number
SG11202007686VA
SG11202007686VA SG11202007686VA SG11202007686VA SG11202007686VA SG 11202007686V A SG11202007686V A SG 11202007686VA SG 11202007686V A SG11202007686V A SG 11202007686VA SG 11202007686V A SG11202007686V A SG 11202007686VA SG 11202007686V A SG11202007686V A SG 11202007686VA
Authority
SG
Singapore
Prior art keywords
cell populations
individual cells
multiple analytes
characterizing multiple
methods characterizing
Prior art date
Application number
SG11202007686VA
Inventor
Zahra Kamila Belhocine
Jason Bell
Zachary Bent
Rajiv Bharadwaj
Christopher Hindson
Lenji Mohammad Rahimi
Bill Kengli Lin
Anthony Makarewicz
Geoffrey Mcdermott
Elliott Meer
Francesca Meschi
Tarjei Sigurd Mikkelsen
Christopher Joachim O'keeffe
Katherine Pfeiffer
Andrew D Price
Paul Ryvkin
Michael Schnall-Levin
Sarah Taylor
Jessica Michele Terry
Tobias Daniel Wheeler
Yifeng Yin
Xinying Zheng
Solongo Batjargal Ziraldo
Eswar Prasad Ramachandran Iyer
Luigi Jhon Alvarado Martinez
Michael Stubbington
Original Assignee
10X Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 10X Genomics Inc filed Critical 10X Genomics Inc
Publication of SG11202007686VA publication Critical patent/SG11202007686VA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5032—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G01N33/5304—Reaction vessels, e.g. agglutination plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/548—Carbohydrates, e.g. dextran
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149—Particles, e.g. beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG11202007686VA 2018-02-12 2019-02-12 Methods characterizing multiple analytes from individual cells or cell populations SG11202007686VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862629602P 2018-02-12 2018-02-12
PCT/US2019/017723 WO2019157529A1 (en) 2018-02-12 2019-02-12 Methods characterizing multiple analytes from individual cells or cell populations

Publications (1)

Publication Number Publication Date
SG11202007686VA true SG11202007686VA (en) 2020-09-29

Family

ID=65529846

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202007686VA SG11202007686VA (en) 2018-02-12 2019-02-12 Methods characterizing multiple analytes from individual cells or cell populations

Country Status (5)

Country Link
US (10) US10725027B2 (en)
EP (1) EP3752832A1 (en)
CN (1) CN112005115A (en)
SG (1) SG11202007686VA (en)
WO (1) WO2019157529A1 (en)

Families Citing this family (321)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013130674A1 (en) 2012-02-27 2013-09-06 Cellular Research, Inc. Compositions and kits for molecular counting
LT3305918T (en) * 2012-03-05 2020-09-25 President And Fellows Of Harvard College Methods for epigenetic sequencing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (en) 2012-08-14 2024-04-30 10X基因组学有限公司 Microcapsule compositions and methods
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN105102697A (en) 2013-02-08 2015-11-25 10X基因组学有限公司 Polynucleotide barcode generation
EP2986741B1 (en) 2013-04-17 2019-07-24 Agency For Science, Technology And Research Method for generating extended sequence reads
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
US9567645B2 (en) 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN118910216A (en) 2014-06-26 2024-11-08 10X基因组学有限公司 Method for analyzing nucleic acid from single cell or cell population
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (en) 2014-06-26 2017-03-27 10X Genomics Inc NUCLEIC ACID SEQUENCE ANALYSIS.
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
DK3530752T3 (en) 2015-04-10 2021-04-26 Spatial Transcriptomics Ab SPACIOUS SEPARATE, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES
AU2016248995B2 (en) 2015-04-17 2022-04-28 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US10988759B2 (en) 2016-01-15 2021-04-27 University Of Washington High throughput protein-protein interaction screening in yeast liquid culture
EP3414341A4 (en) 2016-02-11 2019-10-09 10X Genomics, Inc. SYSTEMS, METHODS, AND MEDIA FOR ASSEMBLING NOVO OF GENOME SEQUENCE DATA OVERALL
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN110225972A (en) 2016-09-15 2019-09-10 奥明塔生物技术公司 Immune group library sequence amplification method and application
ES2961743T3 (en) 2016-09-26 2024-03-13 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification is carried out using blocking property oligonucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP4345172A3 (en) 2017-06-05 2024-07-03 Becton, Dickinson and Company Sample indexing for single cells
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. Methods and systems for sample preparation and analysis
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN111712579B (en) 2017-12-22 2024-10-15 10X基因组学有限公司 Systems and methods for processing nucleic acid molecules from one or more cells
CN112005115A (en) 2018-02-12 2020-11-27 10X基因组学有限公司 Methods to characterize multiple analytes from single cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US20210139985A1 (en) * 2018-04-10 2021-05-13 Board Of Regents, The University Of Texas System Dna-barcoded antigen multimers and methods of use thereof
WO2019204560A1 (en) * 2018-04-18 2019-10-24 The Regents Of The University Of California Method to connect chromatin accessibility and transcriptome
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019217099A1 (en) * 2018-05-08 2019-11-14 President And Fellows Of Harvard College Droplet-based single cell genomic dna sequencing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11414699B2 (en) * 2018-05-15 2022-08-16 Mantra Bio, Inc. Barcode-free single vesicle multiplexed protein and RNA analysis
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11093547B2 (en) * 2018-06-19 2021-08-17 Intel Corporation Data storage based on encoded DNA sequences
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289A1 (en) 2018-08-03 2021-06-09 10X Genomics, Inc. Methods and systems to minimize barcode exchange
US20200048626A1 (en) * 2018-08-07 2020-02-13 Augmenta Bioworks, Inc. Methods for Making Gel Beads and Core and Shell Beads with a Cell
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN112805389B (en) 2018-10-01 2024-08-06 贝克顿迪金森公司 Determine 5' transcript sequence
US11662341B2 (en) 2018-10-10 2023-05-30 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
JP7618548B2 (en) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー Whole-transcriptome analysis of single cells using random priming
SG11202102700TA (en) * 2018-11-30 2021-04-29 Illumina Inc Analysis of multiple analytes using a single assay
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123657A2 (en) 2018-12-11 2020-06-18 10X Genomics, Inc. Methods and devices for detecting and sorting droplets or particles
CN113195717A (en) 2018-12-13 2021-07-30 贝克顿迪金森公司 Selective extension in single cell whole transcriptome analysis
MX2021007292A (en) * 2018-12-18 2021-09-21 Mbl Int Corp COMPOSITIONS OF STREPTAVIDIN-OLIGO CONJUGATES OF VARYING pMHC OCCUPANCY.
WO2020139844A1 (en) 2018-12-24 2020-07-02 10X Genomics, Inc. Devices, systems, and methods for controlling liquid flow
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
ES2989967T3 (en) 2019-01-23 2024-11-28 Becton Dickinson Co Oligonucleotides associated with antibodies
CN113728113A (en) * 2019-02-11 2021-11-30 阿尔缇玛基因组学公司 Nucleic acid analysis method
EP3924505A1 (en) 2019-02-12 2021-12-22 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113747974A (en) 2019-02-28 2021-12-03 10X基因组学有限公司 Apparatus, system, and method for improving droplet formation efficiency
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
US11919002B2 (en) 2019-08-20 2024-03-05 10X Genomics, Inc. Devices and methods for generating and recovering droplets
WO2021046121A1 (en) * 2019-09-03 2021-03-11 The Regents Of The University Of California Method to sequence mrna in single cells in parallel with quantification of intracellular phenotype
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
EP4194855A1 (en) 2019-10-11 2023-06-14 10X Genomics, Inc. Methods for analyte detection and analysis
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021097252A1 (en) * 2019-11-13 2021-05-20 Bradley Bernstein Methylation assays and uses thereof
US12059679B2 (en) 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
EP4065280A1 (en) 2019-11-27 2022-10-05 10X Genomics, Inc. Devices, systems, and methods for generating droplets
CN114761538A (en) * 2019-12-06 2022-07-15 伯乐实验室有限公司 Sample processing barcoded bead compositions, methods, preparations, and systems
WO2021133845A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
FI3891300T3 (en) 2019-12-23 2023-05-10 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
EP4085069A1 (en) 2020-01-03 2022-11-09 The Regents of The University of California Systems and methods for identification of mhc-i peptide epitopes
WO2021141138A1 (en) * 2020-01-10 2021-07-15 シンクサイト株式会社 Novel cellular phenotype screening method
EP4090763B1 (en) 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
AU2021207581A1 (en) 2020-01-13 2022-08-11 Fluent Biosciences Inc. Methods and systems for single cell gene profiling
AU2021207454A1 (en) * 2020-01-13 2022-08-11 Fluent Biosciences Inc. Reverse transcription during template emulsification
EP4090328A4 (en) 2020-01-13 2024-02-14 Fluent Biosciences Inc. EMULSION-BASED ACTIVE SCREENING
US11104961B2 (en) 2020-01-13 2021-08-31 Fluent Biosciences Inc. Single cell sequencing
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12188010B2 (en) 2020-01-29 2025-01-07 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021155181A1 (en) * 2020-01-31 2021-08-05 Auragent Bioscience, Llc Ultrabright fluorescent nanocomposite structures for enhanced fluorescent bioassays
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
EP4103732A4 (en) * 2020-02-12 2024-10-23 Mission Bio, Inc. Methods and systems involving digestible primers for improving single cell multi-omic analysis
EP4104177A1 (en) 2020-02-13 2022-12-21 10X Genomics, Inc. Methods for characterizing cells using gene expression and chromatin accessibility
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115698324A (en) 2020-02-21 2023-02-03 10X基因组学有限公司 Methods and compositions for integrating in situ spatial determination
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US20210270703A1 (en) * 2020-02-28 2021-09-02 10X Genomics, Inc. Method for isolating nuclei and cells from tissues
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
CN115956115A (en) * 2020-04-16 2023-04-11 新格元(南京)生物科技有限公司 Methods and compositions for single cell high throughput target sequencing
WO2021212042A1 (en) * 2020-04-16 2021-10-21 10X Genomics, Inc. Compositions and methods for use with fixed samples
EP4549582A3 (en) 2020-04-22 2025-06-18 10x Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021222301A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for analysis and identification of barcode multiplets
WO2021222302A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for increasing cell recovery efficiency
WO2021226290A1 (en) 2020-05-05 2021-11-11 10X Genomics, Inc. Methods for identification of antigen-binding molecules
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
WO2021231013A1 (en) 2020-05-11 2021-11-18 A-Alpha Bio, Inc. High-throughput screening methods to identify small molecule targets
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
EP4414459A3 (en) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
EP4158052A4 (en) * 2020-05-29 2023-10-18 Siemens Healthcare Diagnostics, Inc. Method of detecting analytes in a sample
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
CN115768889A (en) 2020-06-01 2023-03-07 A-阿尔法生物股份有限公司 Methods of characterizing and engineering protein-protein interactions
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4158058A1 (en) 2020-06-02 2023-04-05 10X Genomics, Inc. Enrichment of nucleic acid sequences
AU2021283184A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
US12263482B1 (en) 2020-06-03 2025-04-01 10X Genomics, Inc. Methods and devices for magnetic separation in a flow path
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
WO2021258024A1 (en) * 2020-06-19 2021-12-23 California Institute Of Technology Sensitive and multiplexed detection of nucleic acids and proteins for large scale serological testing
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
EP4176436A1 (en) 2020-07-02 2023-05-10 10X Genomics, Inc. Systems and methods for detecting cell-associated barcodes from single-cell partitions
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
WO2022006455A1 (en) 2020-07-02 2022-01-06 10X Genomics, Inc. Systems and methods for detection of low-abundance molecular barcodes from a sequencing library
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12241059B2 (en) 2020-07-15 2025-03-04 Illumina, Inc. Tiered ligation oligos
EP4186060A4 (en) 2020-07-23 2024-01-03 10X Genomics, Inc. Systems and methods for detecting and removing aggregates for calling cell-associated barcodes
US20220033810A1 (en) * 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022021279A1 (en) * 2020-07-31 2022-02-03 北京寻因生物科技有限公司 Multi-nucleic acid co-labeling support, preparation method therefor, and application thereof
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4208292B1 (en) 2020-09-02 2024-11-06 10X Genomics, Inc. Flow focusing devices, systems, and methods for high throughput droplet formation
WO2022051529A1 (en) 2020-09-02 2022-03-10 10X Genomics, Inc. Devices, systems, and methods for high throughput droplet formation
CN116097361A (en) 2020-09-04 2023-05-09 10X基因组学有限公司 Systems and methods for identifying feature linkage in multi-genomic feature data from single cell partitions
EP4491742A3 (en) 2020-09-18 2025-05-21 10x Genomics, Inc. Sample handling apparatus and image registration methods
WO2022066760A1 (en) 2020-09-23 2022-03-31 10X Genomics, Inc. Selective enzymatic gelation
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4222279A1 (en) * 2020-09-30 2023-08-09 Guardant Health, Inc. Methods and systems to improve the signal to noise ratio of dna methylation partitioning assays
EP3978901A1 (en) * 2020-10-01 2022-04-06 Siemens Healthcare GmbH Maturity classification of stained reticulocytes using optical microscopy
WO2022076914A1 (en) 2020-10-09 2022-04-14 10X Genomics, Inc. Methods and compositions for profiling immune repertoire
US12298314B2 (en) 2020-10-09 2025-05-13 10X Genomics, Inc. Methods and compositions for analyzing target binding of molecules
EP4225934B1 (en) 2020-10-09 2024-07-24 10X Genomics, Inc. Methods and compositions for analyzing antigen binding molecules
WO2022081643A2 (en) 2020-10-13 2022-04-21 10X Genomics, Inc. Compositions and methods for generating recombinant antigen binding molecules from single cells
AU2021360589A1 (en) * 2020-10-15 2023-06-22 Board Of Regents, The University Of Texas System Detection and analysis of structural variations in genomes
US20240247303A1 (en) * 2021-02-23 2024-07-25 10X Genomics, Inc. Methods and systems for nucleic acid analysis
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022103712A1 (en) 2020-11-13 2022-05-19 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US20220186300A1 (en) 2020-12-11 2022-06-16 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4267760A1 (en) 2020-12-23 2023-11-01 10X Genomics, Inc. Methods and compositions for analyte detection
ES2993714T3 (en) * 2020-12-30 2025-01-07 10X Genomics Inc Molecular array generation using photoresist
EP4274907A4 (en) 2021-01-08 2025-01-15 Cellanome Inc DEVICES AND METHODS FOR THE ANALYSIS OF BIOLOGICAL SAMPLES
EP4275051A1 (en) * 2021-01-08 2023-11-15 10X Genomics, Inc. Methods for generating antigen-binding molecules from single cells
WO2022150659A1 (en) * 2021-01-08 2022-07-14 Cellanome, Inc. Devices and methods for analyzing biological samples
EP4284945B1 (en) 2021-01-26 2024-10-23 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
WO2022178304A1 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. High-throughput methods for analyzing and affinity-maturing an antigen-binding molecule
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022182664A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. A method for epitope binning of novel monoclonal antibodies
WO2022182662A1 (en) * 2021-02-23 2022-09-01 10X Genomics, Inc. Compositions and methods for mapping antigen-binding molecule affinity to antigen regions of interest
EP4298239A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Drug screening methods
CN117178061A (en) 2021-02-23 2023-12-05 10X基因组学有限公司 Single-cell glycan profiling
EP4297901A1 (en) 2021-02-24 2024-01-03 10X Genomics, Inc. Method for concentrating droplets in an emulsion
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
WO2022187366A1 (en) 2021-03-03 2022-09-09 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
ES3008686T3 (en) 2021-03-18 2025-03-24 10X Genomics Inc Multiplex capture of gene and protein expression from a biological sample
WO2022204539A1 (en) 2021-03-26 2022-09-29 10X Genomics, Inc. Devices, methods, and systems for improved droplet recovery
CN113092461B (en) * 2021-04-09 2023-08-22 四川大学华西医院 Homogeneous two-dimensional visualization/fluorescence analysis method and application
WO2022221428A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Compositions and methods for single cell analyte detection and analysis
EP4428246A3 (en) 2021-04-14 2024-11-27 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
IL307845A (en) * 2021-04-21 2023-12-01 Univ Rutgers Identifying microbial signatures and gene expression signatures
CN117337327A (en) * 2021-04-24 2024-01-02 通用测序技术公司 Nanometer train for single molecule detection
WO2022232050A1 (en) * 2021-04-26 2022-11-03 The Broad Institute, Inc. Compositions and methods for characterizing polynucleotide sequence alterations
CN113024665A (en) * 2021-05-31 2021-06-25 泛肽生物科技(浙江)有限公司 Method for labeling antibodies by oligos and antibodies obtained by labeling antibodies
WO2022256345A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Methods and systems for engineering antibodies, and antigen-binding fragments thereof, to have altered characteristics
WO2022256313A1 (en) 2021-06-01 2022-12-08 10X Genomics, Inc. Validation of a unique molecular identifier associated with a nucleic acid sequence of interest
CN117396613A (en) 2021-06-01 2024-01-12 10X基因组学有限公司 Methods and compositions for analyte detection and probe resolution
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022261188A1 (en) * 2021-06-11 2022-12-15 Mission Bio, Inc. Method, system, and apparatus for analyzing an analyte of a single cell
US11535892B1 (en) 2021-06-17 2022-12-27 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2022271908A1 (en) 2021-06-23 2022-12-29 10X Genomics, Inc. Chop-fix method and chopping device for preparing biological samples
WO2023283442A1 (en) 2021-07-09 2023-01-12 10X Genomics, Inc. Methods for detecting analytes using sparse labelling
EP4370896A1 (en) 2021-07-13 2024-05-22 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
WO2023004068A2 (en) 2021-07-21 2023-01-26 10X Genomics, Inc. Methods, devices, and kits for purifying and lysing biological particles
CN117980500A (en) 2021-07-26 2024-05-03 10X基因组学有限公司 Nucleic acid treatment via circularization
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
AU2022317053B2 (en) 2021-07-30 2025-01-02 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
EP4381095A1 (en) 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
EP4326898B1 (en) 2021-08-16 2024-07-31 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4388123B1 (en) 2021-08-17 2025-06-18 10X Genomics, Inc. Compositions, systems and methods for enzyme optimization
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
US20230167496A1 (en) * 2021-10-01 2023-06-01 10X Genomics, Inc. Compositions and methods for isolating nucleic acid molecules
WO2023060110A1 (en) 2021-10-05 2023-04-13 10X Genomics, Inc. Methods of immune cell analysis
EP4413578A1 (en) 2021-10-06 2024-08-14 10X Genomics, Inc. Systems and methods for evaluating biological samples
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
AU2022387613A1 (en) 2021-11-10 2024-05-30 10X Genomics, Inc. Methods for identification of antigen-binding molecules
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023108139A2 (en) 2021-12-10 2023-06-15 10X Genomics, Inc. Multi-resolution in situ decoding
WO2023114310A1 (en) 2021-12-15 2023-06-22 10X Genomics, Inc. Methods for improving sensitivity of immune profiling using oligo-tagged antigens
WO2023122033A1 (en) 2021-12-20 2023-06-29 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US20230242974A1 (en) 2021-12-27 2023-08-03 10X Genomics, Inc. Methods and compositions for rolling circle amplification
WO2023137583A1 (en) * 2022-01-18 2023-07-27 深圳华大生命科学研究院 Method for determining effect relationship between various substances and cells, and microwell array chip
US20230279475A1 (en) 2022-01-21 2023-09-07 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023168423A1 (en) 2022-03-04 2023-09-07 10X Genomics, Inc. Droplet forming devices and methods having fluoropolymer silane coating agents
CN118974836A (en) 2022-03-08 2024-11-15 10X基因组学有限公司 In-situ code design method to minimize optical crowding
WO2023192302A1 (en) 2022-03-29 2023-10-05 10X Genomics, Inc. Spectral unmixing combined with decoding for super-multiplexed in situ analysis
WO2023192616A1 (en) 2022-04-01 2023-10-05 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
EP4504969A1 (en) 2022-04-06 2025-02-12 10X Genomics, Inc. Methods for multiplex cell analysis
WO2023201235A2 (en) 2022-04-12 2023-10-19 10X Genomics, Inc. Compositions and methods for generating and characterizing recombinant antigen binding molecules
WO2023212532A1 (en) 2022-04-26 2023-11-02 10X Genomics, Inc. Systems and methods for evaluating biological samples
WO2023215861A1 (en) 2022-05-06 2023-11-09 10X Genomics, Inc. Reagents for characterizing antigen-binding molecules from immune cells
US20240084378A1 (en) 2022-05-11 2024-03-14 10X Genomics, Inc. Compositions and methods for in situ sequencing
CN114624225B (en) * 2022-05-16 2022-07-29 南京邮电大学 Membrane protein dimer in-situ detection kit and application thereof
WO2023225294A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Improved major histocompatibility complex molecules
WO2023225259A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Compositions and methods for characterizing antigen binding molecules from single cells
WO2023225201A1 (en) 2022-05-20 2023-11-23 10X Genomics, Inc. Compositions and methods for characterizing t cell, or t cell-like, receptors from single cells
CN114934096A (en) * 2022-05-31 2022-08-23 上海市生物医药技术研究院 Compositions and kits for performing immune repertoire sequencing and methods of sequencing
WO2023235570A1 (en) 2022-06-03 2023-12-07 10X Genomics, Inc. Methods and compositions for the identification of antigen binding molecules using lipoparticle-based antigen mapping
WO2023235596A1 (en) 2022-06-03 2023-12-07 10X Genomics, Inc. Systems and methods for determining antigen binding specificity of antigen binding molecules
WO2023239907A1 (en) * 2022-06-09 2023-12-14 The Regents Of The University Of California Single cell co-sequencing of dna methylation and rna
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
WO2023250422A1 (en) 2022-06-23 2023-12-28 10X Genomics, Inc. Compositions and methods for characterizing multispecific antigen binding molecules from single cells
WO2024006734A1 (en) 2022-06-27 2024-01-04 10X Genomics, Inc. Methods for preparing and using mhc multimer reagents compositions
CN115050417B (en) * 2022-06-27 2024-07-19 华南农业大学 Method for predicting chromatin domain structure TAD based on chromatin random folding process
US20240084359A1 (en) 2022-06-29 2024-03-14 10X Genomics, Inc. Methods and compositions for patterned molecular array generation by directed bead delivery
WO2024006392A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2024015856A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Compositions and methods for characterizing binding characteristics of antigen binding molecules from single cells
WO2024015378A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods and systems for characterizing antigen-binding molecules expressed by immune cells
WO2024015862A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Methods for characterization of antigen-binding molecules from biological samples
WO2024015733A1 (en) 2022-07-13 2024-01-18 10X Genomics, Inc. Improved methods and systems for identification and characterization of antigen-binding molecules from single cells
CN119630789A (en) * 2022-08-12 2025-03-14 美天施生物科技有限两合公司 Barcoding of nucleic acids from single cells
US20240101978A1 (en) 2022-08-12 2024-03-28 10X Genomics, Inc. Puma1 polymerases and uses thereof
EP4511510A1 (en) 2022-08-16 2025-02-26 10X Genomics, Inc. Ap50 polymerases and uses thereof
CN118201982A (en) 2022-08-18 2024-06-14 10X基因组学有限公司 Droplet forming apparatus and method with fluorine-containing glycol additive
WO2024044703A1 (en) 2022-08-24 2024-02-29 10X Genomics, Inc. Compositions and methods for antigenic epitope mapping in biological samples
WO2024050299A1 (en) 2022-08-29 2024-03-07 10X Genomics, Inc. Improved methods and compositions for characterization of antigen-binding molecules from single cells
EP4332234A1 (en) * 2022-08-29 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) Improved microfluidic transcriptome based screening methods
WO2024076908A1 (en) 2022-10-03 2024-04-11 10X Genomics, Inc. Compositions and methods for analyzing genomic insertion sites of exogenous nucleic acids
US20240279648A1 (en) * 2022-10-06 2024-08-22 Fluent Biosciences Inc. Quantitative detection and analysis of molecules
CN120129825A (en) 2022-10-14 2025-06-10 10X基因组学有限公司 Methods, compositions and systems for assessing the quality of biological samples
WO2024097719A1 (en) * 2022-11-02 2024-05-10 Becton, Dickinson And Company Application for peptide nucleic acid (pna) blocker
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
US20240218424A1 (en) 2022-12-21 2024-07-04 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
WO2024163787A1 (en) * 2023-02-01 2024-08-08 University Hospitals Cleveland Medical Center Crispr technologies for diagnostic applications
WO2024229260A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
US20240376521A1 (en) 2023-05-10 2024-11-14 10X Genomics, Inc. Boronic acid compositions and methods for tethering ribonucleic acids in biological samples
WO2024233949A1 (en) * 2023-05-11 2024-11-14 Replace Therapeutics, Llc Revision of genetic material using direct replacement editing
US20250012786A1 (en) 2023-07-07 2025-01-09 10X Genomics, Inc. Systems and methods for tissue bounds detection
WO2025038914A1 (en) 2023-08-16 2025-02-20 10X Genomics, Inc. Systems and methods for image segmentation
CN118647729A (en) * 2023-09-18 2024-09-13 中国科学院北京基因组研究所(国家生物信息中心) Method and kit for high-throughput labeling of cellular nucleic acid molecules
WO2025064703A1 (en) 2023-09-21 2025-03-27 10X Genomics, Inc. Compositions and methods for particle hashing
WO2025076421A1 (en) 2023-10-06 2025-04-10 10X Genomics, Inc. Feature pyramiding for in situ data visualizations
WO2025101377A1 (en) 2023-11-09 2025-05-15 10X Genomics, Inc. Matrix-assisted spatial analysis of biological samples
WO2025111369A1 (en) 2023-11-21 2025-05-30 10X Genomics, Inc. Probe-based analysis of polynucleotides and proteins
WO2025111463A1 (en) 2023-11-22 2025-05-30 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
US20250188524A1 (en) 2023-12-07 2025-06-12 10X Genomics, Inc. Graphical user interface and method of estimating an instrument run completion time
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
CN118727162B (en) * 2024-06-05 2025-04-04 首都医科大学附属北京口腔医院 Single cell 4C library construction and detection methods

Family Cites Families (453)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1059024A (en) 1912-06-03 1913-04-15 William T Beyer Insect-destroyer.
US4124638A (en) 1977-09-12 1978-11-07 Hansen John N Solubilizable polyacrylamide gels containing disulfide cross-linkages
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
US5137829A (en) 1987-10-05 1992-08-11 Washington University DNA transposon TN5SEQ1
US5185099A (en) 1988-04-20 1993-02-09 Institut National De Recherche Chimique Appliquee Visco-elastic, isotropic materials based on water, fluorinate sufactants and fluorinated oils, process for their preparation, and their use in various fields, such as optics, pharmacology and electrodynamics
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US6176962B1 (en) 1990-02-28 2001-01-23 Aclara Biosciences, Inc. Methods for fabricating enclosed microchannel structures
US5756334A (en) 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69213240T2 (en) 1991-07-04 1997-04-24 Immunodex K/S, Glostrup WATER-SOLUBLE REAGENTS AND POLYMER-BASED CONJUGATES CONTAINING REMAINS DERIVED FROM THE DIVINYL SULPHONE
US5569364A (en) 1992-11-05 1996-10-29 Soane Biosciences, Inc. Separation media for electrophoresis
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
DE69434520T3 (en) 1993-07-30 2009-10-15 Affymax, Inc., Palo Alto BIOTINYLATION OF PROTEINS
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
AU718357B2 (en) 1995-06-07 2000-04-13 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US5965443A (en) 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
WO1998022625A1 (en) 1996-11-20 1998-05-28 The Regents Of The University Of Michigan Microfabricated isothermal nucleic acid amplification devices and methods
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
AU736321B2 (en) 1997-05-23 2001-07-26 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US20040241759A1 (en) 1997-06-16 2004-12-02 Eileen Tozer High throughput screening of libraries
EP1019496B1 (en) 1997-07-07 2004-09-29 Medical Research Council In vitro sorting method
US20050037397A1 (en) 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20020092767A1 (en) 1997-09-19 2002-07-18 Aclara Biosciences, Inc. Multiple array microfluidic device units
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
IL137706A0 (en) 1998-02-19 2001-10-31 Harvard College Mhc fusion proteins and conjugates
WO1999050402A1 (en) 1998-03-27 1999-10-07 President And Fellows Of Harvard College Systematic identification of essential genes by in vitro transposon mutagenesis
EP1079967A4 (en) 1998-04-13 2003-07-23 Luminex Corp Liquid labeling with fluorescent microparticles
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6123798A (en) 1998-05-06 2000-09-26 Caliper Technologies Corp. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US6586176B1 (en) 1998-08-07 2003-07-01 Cellay, Llc Gel microdrops in genetic analysis
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
US5942609A (en) 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US20030027214A1 (en) 1999-02-17 2003-02-06 Kamb Carl Alexander Methods for substrate-ligand interaction screening
US6613513B1 (en) 1999-02-23 2003-09-02 Caliper Technologies Corp. Sequencing by incorporation
US6171850B1 (en) 1999-03-08 2001-01-09 Caliper Technologies Corp. Integrated devices and systems for performing temperature controlled reactions and analyses
EP1163519B1 (en) * 1999-03-23 2006-07-26 Biovation Limited Protein isolation and analysis
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
HK1046156B (en) 1999-04-20 2009-01-23 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
US20030124509A1 (en) 1999-06-03 2003-07-03 Kenis Paul J.A. Laminar flow patterning and articles made thereby
US6613580B1 (en) 1999-07-06 2003-09-02 Caliper Technologies Corp. Microfluidic systems and methods for determining modulator kinetics
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
EP1248853A2 (en) 1999-08-20 2002-10-16 Luminex Corporation Liquid array technology
ATE414171T1 (en) 1999-08-27 2008-11-15 Matrix Technologies Corp METHOD FOR IMMOBILIZING OLIGONUCLEOTIDES ON SOLID SUPPORT MATERIAL
AU2001238068A1 (en) 2000-02-07 2001-08-14 Illumina, Inc. Nucleic acid detection methods using universal priming
US6915679B2 (en) 2000-02-23 2005-07-12 Caliper Life Sciences, Inc. Multi-reservoir pressure control system
CA2404008A1 (en) 2000-03-31 2001-10-11 Jurgen Sygusch Protein crystallization in microfluidic structures
US20060008799A1 (en) 2000-05-22 2006-01-12 Hong Cai Rapid haplotyping by single molecule detection
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6410246B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
WO2002029106A2 (en) 2000-10-03 2002-04-11 California Institute Of Technology Microfluidic devices and methods of use
US7083787B2 (en) 2000-11-15 2006-08-01 Globeimmune, Inc. Yeast-dendritic cell vaccines and uses thereof
US7670559B2 (en) 2001-02-15 2010-03-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
DE60214604T2 (en) 2001-02-23 2006-12-28 Japan Science And Technology Agency, Kawaguchi Apparatus and method for producing microcapsules
JP4608184B2 (en) 2001-03-14 2011-01-05 ダコ デンマーク アクティーゼルスカブ Novel MHC molecule constructs and methods of using these constructs for diagnosis and treatment, and use of MHC molecules
US20150329617A1 (en) 2001-03-14 2015-11-19 Dynal Biotech Asa Novel MHC molecule constructs, and methods of employing these constructs for diagnosis and therapy, and uses of MHC molecules
WO2005003394A2 (en) * 2003-06-27 2005-01-13 Nanosphere, Inc. Bio-barcode based detection of target analytes
US7138267B1 (en) 2001-04-04 2006-11-21 Epicentre Technologies Corporation Methods and compositions for amplifying DNA clone copy number
JP4194934B2 (en) 2001-05-26 2008-12-10 ワン セル システムズ インコーポレイテッド Protein secretion by encapsulated cells
DE60232672D1 (en) 2001-10-01 2009-07-30 Dyax Corp MULTILACKED EUKARYONTIC DISPLAY VECTORS AND THEIR USES
CA2464350A1 (en) 2001-10-19 2003-05-30 West Virginia University Research Corporation Microfluidic system for proteome analysis
US6783647B2 (en) 2001-10-19 2004-08-31 Ut-Battelle, Llc Microfluidic systems and methods of transport and lysis of cells and analysis of cell lysate
US7262056B2 (en) 2001-11-08 2007-08-28 Mirus Bio Corporation Enhancing intermolecular integration of nucleic acids using integrator complexes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7335153B2 (en) 2001-12-28 2008-02-26 Bio Array Solutions Ltd. Arrays of microparticles and methods of preparation thereof
WO2004038363A2 (en) 2002-05-09 2004-05-06 The University Of Chicago Microfluidic device and method for pressure-driven plug transport and reaction
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US20050266582A1 (en) 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US7700325B2 (en) 2003-01-17 2010-04-20 Trustees Of Boston University Haplotype analysis
US7244567B2 (en) 2003-01-29 2007-07-17 454 Life Sciences Corporation Double ended sequencing
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20100035254A1 (en) 2003-04-08 2010-02-11 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
EP1633875B1 (en) 2003-05-28 2012-05-02 Basf Se Wheat plants having increased tolerance to imidazolinone herbicides
WO2005010145A2 (en) 2003-07-05 2005-02-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP2662135A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for mixing droplets in a microchannel
WO2005023427A1 (en) 2003-09-05 2005-03-17 Stokes Bio Limited A microfluidic analysis system
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND METHODS FOR IN VITRO / I SORTING OF MOLECULAR AND CELLULAR BANKS
CA2553833C (en) 2004-01-28 2012-10-02 454 Corporation Nucleic acid amplification with continuous flow emulsion
US20100216153A1 (en) 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
CA2557841A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
WO2005089921A1 (en) 2004-03-23 2005-09-29 Japan Science And Technology Agency Method and device for producing micro-droplets
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2005111242A2 (en) 2004-05-10 2005-11-24 Parallele Bioscience, Inc. Digital profiling of polynucleotide populations
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7608434B2 (en) 2004-08-04 2009-10-27 Wisconsin Alumni Research Foundation Mutated Tn5 transposase proteins and the use thereof
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20080213593A1 (en) 2005-01-21 2008-09-04 President And Fellows Of Harvard College Systems And Methods For Forming Fluidic Droplets Encapsulated In Particles Such As Colloidal Particles
US7579153B2 (en) 2005-01-25 2009-08-25 Population Genetics Technologies, Ltd. Isothermal DNA amplification
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US8445194B2 (en) 2005-06-15 2013-05-21 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
WO2007120265A2 (en) 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
CA2636855C (en) 2006-01-11 2016-09-27 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
DE602007009811D1 (en) 2006-01-27 2010-11-25 Harvard College COALESCENCE FLUIDER DROPLET
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7811603B2 (en) 2006-05-09 2010-10-12 The Regents Of The University Of California Microfluidic device for forming monodisperse lipoplexes
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
CA2653321A1 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc. Biochemical analysis of partitioned cells
AU2007260676A1 (en) 2006-06-14 2007-12-21 Artemis Health, Inc. Rare cell analysis using sample splitting and DNA tags
US8394590B2 (en) 2006-08-02 2013-03-12 California Institute Of Technology Capture agents and related methods and systems for detecting and/or sorting targets
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
WO2008030433A2 (en) 2006-09-06 2008-03-13 Canon U.S. Life Sciences, Inc. Chip and cartridge design configuration for performing micro-fluidic assays
US7935518B2 (en) 2006-09-27 2011-05-03 Alessandra Luchini Smart hydrogel particles for biomarker harvesting
US20080166720A1 (en) 2006-10-06 2008-07-10 The Regents Of The University Of California Method and apparatus for rapid nucleic acid analysis
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
EP2091647A2 (en) 2006-11-14 2009-08-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8003312B2 (en) 2007-02-16 2011-08-23 The Board Of Trustees Of The Leland Stanford Junior University Multiplex cellular assays using detectable cell barcodes
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
EP2164985A4 (en) 2007-06-01 2014-05-14 454 Life Sciences Corp System and meth0d for identification of individual samples from a multiplex mixture
WO2008148200A1 (en) 2007-06-05 2008-12-11 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
US8454906B2 (en) 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
US20100086914A1 (en) 2008-10-03 2010-04-08 Roche Molecular Systems, Inc. High resolution, high throughput hla genotyping by clonal sequencing
US8334013B2 (en) 2007-11-02 2012-12-18 Stc.Unm Mesoporous metal oxide microspheres and method for forming same
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN101946010B (en) 2007-12-21 2014-08-20 哈佛大学 Systems and methods for nucleic acid sequencing
US9034580B2 (en) 2008-01-17 2015-05-19 Sequenom, Inc. Single molecule nucleic acid sequence analysis processes and compositions
US9011777B2 (en) 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
DE102008025656B4 (en) 2008-05-28 2016-07-28 Genxpro Gmbh Method for the quantitative analysis of nucleic acids, markers therefor and their use
GB0810051D0 (en) 2008-06-02 2008-07-09 Oxford Biodynamics Ltd Method of diagnosis
US20110305761A1 (en) 2008-06-05 2011-12-15 President And Fellows Of Harvard College Polymersomes, colloidosomes, liposomes, and other species associated with fluidic droplets
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
WO2010009735A2 (en) 2008-07-23 2010-01-28 Dako Denmark A/S Combinatorial analysis and repair
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CA2750054C (en) 2008-10-24 2018-05-29 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB2467704B (en) 2008-11-07 2011-08-24 Mlc Dx Inc A method for determining a profile of recombined DNA sequences in T-cells and/or B-cells
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
BRPI1008965B1 (en) 2009-03-13 2018-12-18 Harvard College method for scaling up microfluidic devices and system for droplet formation in parallel microfluidic channels
ES2555389T3 (en) 2009-03-30 2015-12-30 Illumina, Inc. Analysis of gene expression in individual cells
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
WO2010148039A2 (en) 2009-06-15 2010-12-23 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
EP2467479B1 (en) 2009-08-20 2016-01-06 Population Genetics Technologies Ltd Compositions and methods for intramolecular nucleic acid rearrangement
BR112012004719A2 (en) 2009-09-02 2016-04-05 Harvard College multiple emulsions created by blasting and other techniques
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
CN102648053B (en) 2009-10-27 2016-04-27 哈佛学院院长等 Drop formation technology
EP2504448B1 (en) 2009-11-25 2016-10-19 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2794522C (en) 2010-04-05 2019-11-26 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20130261196A1 (en) 2010-06-11 2013-10-03 Lisa Diamond Nucleic Acids For Multiplex Organism Detection and Methods Of Use And Making The Same
WO2012038839A2 (en) 2010-09-21 2012-03-29 Population Genetics Technologies Ltd. Increasing confidence of allele calls with molecular counting
US9999886B2 (en) 2010-10-07 2018-06-19 The Regents Of The University Of California Methods and systems for on demand droplet generation and impedance based detection
US10392726B2 (en) 2010-10-08 2019-08-27 President And Fellows Of Harvard College High-throughput immune sequencing
GB2497912B (en) 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
CA3215088A1 (en) 2010-11-01 2012-05-10 Bio-Rad Laboratories, Inc. System for forming emulsions
AU2011323107B2 (en) 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
EP2652155B1 (en) 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
ES2568910T3 (en) 2011-01-28 2016-05-05 Illumina, Inc. Oligonucleotide replacement for libraries labeled at two ends and addressed
EP2670863B1 (en) 2011-01-31 2018-06-27 H. Hoffnabb-La Roche Ag Methods of identifying multiple epitopes in cells
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
EP2673382B1 (en) 2011-02-11 2020-05-06 Bio-Rad Laboratories, Inc. Thermocycling device for nucleic acid amplification and methods of use
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
EP2678449A4 (en) 2011-02-25 2015-06-24 Illumina Inc Methods and systems for haplotype determination
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN103843001B (en) 2011-04-14 2017-06-09 考利达基因组股份有限公司 The treatment and analysis of complex nucleic acid sequence data
CN103717749A (en) 2011-04-25 2014-04-09 伯乐生命医学产品有限公司 Methods and compositions for nucleic acid analysis
EP3495497B1 (en) 2011-04-28 2021-03-24 Life Technologies Corporation Methods and compositions for multiplex pcr
BR112013029729A2 (en) 2011-05-23 2017-01-24 Basf Se emulsion control including multiple emulsions
US9005935B2 (en) 2011-05-23 2015-04-14 Agilent Technologies, Inc. Methods and compositions for DNA fragmentation and tagging by transposases
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8927218B2 (en) 2011-06-27 2015-01-06 Flir Systems, Inc. Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
US8975302B2 (en) 2011-07-07 2015-03-10 Life Technologies Corporation Polymer particles, nucleic acid polymer particles and methods of making and using the same
US20130017978A1 (en) * 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
HK1199745A1 (en) 2011-09-09 2015-07-17 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
CN106423314B (en) 2011-09-28 2021-03-02 哈佛学院院长等 Systems and methods for droplet generation and/or fluid manipulation
US9514272B2 (en) 2011-10-12 2016-12-06 Complete Genomics, Inc. Identification of DNA fragments and structural variations
EP2773327A4 (en) 2011-11-02 2015-06-03 Univ California NEW PROGRAMMING OF CELL ADHESION
US9938524B2 (en) 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
EP2783001B1 (en) * 2011-11-22 2018-01-03 Active Motif Multiplex isolation of protein-associated nucleic acids
US10689643B2 (en) 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP4249605B1 (en) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Methods for analyte detection
WO2013096643A1 (en) 2011-12-23 2013-06-27 Gigagen Methods and apparatuses for droplet mixing
WO2013126741A1 (en) 2012-02-24 2013-08-29 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
LT3305918T (en) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Methods for epigenetic sequencing
JP6302847B2 (en) 2012-03-05 2018-03-28 アダプティヴ バイオテクノロジーズ コーポレーション Determination of paired immunoreceptor chains from frequency matched subunits
NO2694769T3 (en) 2012-03-06 2018-03-03
AU2013273987B2 (en) 2012-06-15 2018-08-09 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
CA2875695C (en) 2012-06-15 2022-11-15 The Board Of Regents Of The University Of Texas System High throughput sequencing of multiple transcripts of a single cell
EP2882872B1 (en) 2012-08-13 2021-10-06 The Regents of The University of California Methods and systems for detecting biological components
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
CN111748607B (en) 2012-08-14 2024-04-30 10X基因组学有限公司 Microcapsule compositions and methods
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) * 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2898096B1 (en) 2012-09-21 2024-02-14 The Broad Institute, Inc. Methods for labeling of rnas
US9644199B2 (en) 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
WO2014060483A1 (en) 2012-10-17 2014-04-24 Spatial Transcriptomics Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
PT2771468E (en) 2012-12-12 2015-06-02 Harvard College Engineering of systems, methods and optimized guide compositions for sequence manipulation
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2948703B1 (en) 2013-01-25 2019-03-13 Bio-Rad Laboratories, Inc. System and method for performing droplet inflation
US11110458B2 (en) 2013-02-01 2021-09-07 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
CN105102697A (en) 2013-02-08 2015-11-25 10X基因组学有限公司 Polynucleotide barcode generation
US20160008778A1 (en) 2013-03-06 2016-01-14 President And Fellows Of Harvard College Devices and methods for forming relatively monodisperse droplets
CN108441541A (en) 2013-03-08 2018-08-24 伯乐生命医学产品有限公司 Composition, the method and system measured for polymerase chain reaction
DK2970951T3 (en) 2013-03-13 2019-05-13 Illumina Inc PROCEDURES FOR NUCLEAR ACID SEQUENCE
US10612088B2 (en) 2013-03-14 2020-04-07 The Broad Institute, Inc. Massively multiplexed RNA sequencing
AU2014233373B2 (en) 2013-03-15 2019-10-24 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
US20140272996A1 (en) 2013-03-15 2014-09-18 Bio-Rad Laboratories, Inc. Droplet generator with collection tube
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
HK1221013A1 (en) 2013-03-15 2017-05-19 普罗格诺西斯生物科学公司 Methods for detecting peptide/mhc/tcr binding
SG11201506991VA (en) 2013-03-15 2015-10-29 Adaptive Biotechnologies Corp Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
EP2970877B1 (en) * 2013-03-15 2018-07-25 Wake Forest University Health Sciences Neural progenitor cell differentiation
CA2906076A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
EP2981349B1 (en) 2013-04-02 2025-02-05 Bio-Rad Laboratories, Inc. Systems for handling microfluidic droplets
CN105431575B (en) 2013-05-09 2017-08-29 生物辐射实验室股份有限公司 Magnetic immuno digital pcr is tested
CN111394426B (en) 2013-05-23 2024-05-10 斯坦福大学托管董事会 Transposition to native chromatin for personal epigenomics
US20160122753A1 (en) 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
EP3587585B1 (en) 2013-06-12 2021-03-24 The General Hospital Corporation Methods, kits, and systems for multiplexed detection of target molecules and uses thereof
SG10201710486XA (en) 2013-06-17 2018-01-30 Broad Inst Inc Delivery and Use of the Crispr-Cas Systems, Vectors and Compositions for Hepatic Targeting and Therapy
WO2014205296A1 (en) 2013-06-21 2014-12-24 The Broad Institute, Inc. Methods for shearing and tagging dna for chromatin immunoprecipitation and sequencing
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
CA2915499A1 (en) * 2013-06-27 2014-12-31 10X Genomics, Inc. Compositions and methods for sample processing
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
GB2516684A (en) 2013-07-30 2015-02-04 Sphere Fluidics Ltd Microfluidic devices and systems
US9567645B2 (en) 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
ES2875998T3 (en) 2013-09-30 2021-11-11 Vesicode Ab Procedures for profiling molecular complexes using proximity-dependent barcodes
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
AU2014364926B2 (en) 2013-12-20 2017-12-14 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
US9580736B2 (en) * 2013-12-30 2017-02-28 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
WO2015123588A1 (en) 2014-02-13 2015-08-20 Bio-Rad Laboratories, Inc. Chromosome conformation capture in partitions
US10597698B2 (en) 2014-03-03 2020-03-24 The Board Of Trustees Of The University Of Illinois Chromatin immunocapture devices and methods of use
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
DE202015009494U1 (en) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidic devices and systems for encapsulating and partitioning reagents, and their applications
CN113005108B (en) 2014-04-15 2025-02-11 伊鲁米那股份有限公司 Modified transposases for improved insertion sequence bias and increased DNA input tolerance
CN107075543B (en) * 2014-04-21 2021-11-16 哈佛学院院长及董事 Systems and methods for barcoding nucleic acids
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
EP3146046B1 (en) * 2014-05-23 2020-03-11 Digenomix Corporation Haploidome determination by digitized transposons
EP3628684B1 (en) * 2014-06-06 2024-09-18 Herlev Hospital Determining antigen recognition through barcoding of mhc multimers
WO2015191877A1 (en) 2014-06-11 2015-12-17 Life Technologies Corporation Systems and methods for substrate enrichment
US11585806B2 (en) 2014-06-13 2023-02-21 Immudex Aps General detection and isolation of specific cells by binding of labeled molecules
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
HK1219116A1 (en) 2014-06-24 2017-03-24 生物辐射实验室股份有限公司 Digital pcr barcoding
US20150376605A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods and Compositions for Sample Analysis
CN106575322B (en) 2014-06-26 2019-06-18 10X基因组学有限公司 The method and system of nucleic acid sequence assembly
CN118910216A (en) 2014-06-26 2024-11-08 10X基因组学有限公司 Method for analyzing nucleic acid from single cell or cell population
MX2016016904A (en) 2014-06-26 2017-03-27 10X Genomics Inc NUCLEIC ACID SEQUENCE ANALYSIS.
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
US20160024558A1 (en) 2014-07-23 2016-01-28 10X Genomics, Inc. Nucleic acid binding proteins and uses thereof
JP2017532024A (en) 2014-09-09 2017-11-02 ザ・ブロード・インスティテュート・インコーポレイテッド Droplet-based methods and instruments for composite single cell nucleic acid analysis
RU2709655C2 (en) 2014-10-17 2019-12-19 Иллумина Кембридж Лимитед Transposition with preservation of gene adhesion
CN107002128A (en) 2014-10-29 2017-08-01 10X 基因组学有限公司 The method and composition being sequenced for target nucleic acid
WO2016069886A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corporation Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
BR112017014902A2 (en) 2015-01-12 2018-03-13 10X Genomics Inc processes and systems for the preparation of nucleic acid sequencing libraries and libraries prepared using them
AU2016206706B2 (en) 2015-01-13 2021-10-07 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
ES2784361T3 (en) 2015-01-22 2020-09-24 Becton Dickinson Co Devices and systems for creating molecular barcodes of nucleic acid targets in single cells
JP6585175B2 (en) 2015-01-30 2019-10-02 アンテローム Host DNA as a biomarker for Crohn's disease
US11111519B2 (en) * 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
MX2017010142A (en) 2015-02-09 2017-12-11 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data.
RU2761432C2 (en) 2015-02-10 2021-12-08 Иллюмина, Инк. Method and composition for analysis of cellular components
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
ES2906221T3 (en) 2015-02-27 2022-04-13 Becton Dickinson Co Methods for barcoding nucleic acids for sequencing
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2016160908A1 (en) 2015-03-30 2016-10-06 Verily Life Sciences Llc Methods for combining single cell profiling with combinatorial nanoparticle conjugate library screening
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
DK3530752T3 (en) 2015-04-10 2021-04-26 Spatial Transcriptomics Ab SPACIOUS SEPARATE, MULTIPLEX NUCLEIC ACID ANALYSIS OF BIOLOGICAL SAMPLES
AU2016248995B2 (en) 2015-04-17 2022-04-28 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US20160314242A1 (en) 2015-04-23 2016-10-27 10X Genomics, Inc. Sample indexing methods and compositions for sequencing applications
JP6894380B2 (en) 2015-04-27 2021-06-30 アブビトロ， エルエルシー Methods for Sequencing, Determining, Pairing and Verifying Therapeutic Drugs and Disease-Specific Antigens
WO2016174229A1 (en) 2015-04-30 2016-11-03 European Molecular Biology Laboratory Microfluidic droplet detection and sorting
EP3298168A4 (en) 2015-05-18 2019-02-20 10X Genomics, Inc. STABILIZED REDUCING AGENTS AND METHODS OF USE
JP2018518950A (en) 2015-05-18 2018-07-19 １０エックス ジェノミクス， インコーポレイテッド Mobile solid phase composition for use in biomedical reactions and analysis
US20180087050A1 (en) 2015-05-27 2018-03-29 Jianbiao Zheng Methods of inserting molecular barcodes
AU2016279062A1 (en) * 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
NZ738452A (en) 2015-06-24 2025-05-02 Oxford BioDynamics PLC Epigenetic chromosome interactions
EP3314020A1 (en) 2015-06-29 2018-05-02 The Broad Institute Inc. Tumor and microenvironment gene expression, compositions of matter and methods of use thereof
US10894980B2 (en) 2015-07-17 2021-01-19 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
WO2017013170A1 (en) * 2015-07-22 2017-01-26 F. Hoffmann-La Roche Ag Identification of antigen epitopes and immune sequences recognizing the antigens
EP3674414A1 (en) * 2015-08-12 2020-07-01 CeMM - Forschungszentrum für Molekulare Medizin GmbH Methods for studying nucleic acids
US11479805B2 (en) 2015-08-21 2022-10-25 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
BR112018005937A2 (en) * 2015-09-24 2019-05-21 Abvitro Llc affinity oligonucleotide conjugates and uses of these
CA2999886A1 (en) 2015-09-24 2017-03-30 Abvitro Llc Single amplicon activated exclusion pcr
WO2017053902A1 (en) 2015-09-25 2017-03-30 Abvitro Llc High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
CA3001679A1 (en) 2015-10-13 2017-04-20 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
CN108368522A (en) 2015-10-20 2018-08-03 10X 基因组学有限公司 Method and system for high-throughput unicellular genetic manipulation
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017087910A1 (en) 2015-11-19 2017-05-26 10X Genomics, Inc. Transformable tagging compositions, methods, and processes incorporating same
JP6954899B2 (en) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド Methods and compositions for nucleic acid analysis
US11965891B2 (en) * 2015-12-30 2024-04-23 Bio-Rad Laboratories, Inc. Digital protein quantification
EP3414341A4 (en) 2016-02-11 2019-10-09 10X Genomics, Inc. SYSTEMS, METHODS, AND MEDIA FOR ASSEMBLING NOVO OF GENOME SEQUENCE DATA OVERALL
WO2017139690A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Cell population analysis using single nucleotide polymorphisms from single cell transcriptomes
WO2017145476A1 (en) 2016-02-26 2017-08-31 富士フイルム株式会社 Method for producing lithographic printing plate
US11680253B2 (en) 2016-03-10 2023-06-20 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
US20170283864A1 (en) 2016-03-31 2017-10-05 Agilent Technologies, Inc. Use of transposase and y adapters to fragment and tag dna
CA3020913C (en) 2016-04-15 2024-01-02 President And Fellows Of Harvard College Systems and methods for the collection of droplets and/or other entities
US20190127789A1 (en) 2016-04-19 2019-05-02 President And Fellows Of Harvard College Immobilization-based systems and methods for genetic analysis and other applications
US12123878B2 (en) 2016-05-02 2024-10-22 Encodia, Inc. Macromolecule analysis employing nucleic acid encoding
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018031631A1 (en) 2016-08-10 2018-02-15 President And Fellows Of Harvard College Methods of de novo assembly of barcoded genomic dna fragments
WO2018039338A1 (en) 2016-08-23 2018-03-01 10X Genomics, Inc. Microfluidic surface-mediated emulsion stability control
EP3507379A4 (en) 2016-08-31 2020-05-13 President and Fellows of Harvard College METHODS FOR DIGITAL AMPLIFICATION OF THE WHOLE GENOME
US20180080021A1 (en) 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
JP6929354B2 (en) 2016-09-24 2021-09-01 アブビトロ， エルエルシー Affinity-oligonucleotide conjugates and their use
ES2961743T3 (en) 2016-09-26 2024-03-13 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
CN110114520B (en) 2016-10-01 2023-08-08 伯克利之光生命科技公司 DNA barcode compositions and methods of in situ identification in microfluidic devices
WO2018075693A1 (en) 2016-10-19 2018-04-26 10X Genomics, Inc. Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
US10656144B2 (en) 2016-12-02 2020-05-19 The Charlotte Mecklenburg Hospital Authority Immune profiling and minimal residue disease following stem cell transplantation in multiple myeloma
ES2922281T3 (en) 2016-12-07 2022-09-12 Mgi Tech Co Ltd Method for constructing a single cell sequencing library and use thereof
CN118441026A (en) 2016-12-19 2024-08-06 生物辐射实验室股份有限公司 Drop-on labeled DNA with maintained adjacency
EP3571308A4 (en) 2016-12-21 2020-08-19 The Regents of The University of California SINGLE CELL GENOMIC SEQUENCING WITH DROPLETS BASED ON HYDROGEL
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20190177800A1 (en) 2017-12-08 2019-06-13 10X Genomics, Inc. Methods and compositions for labeling cells
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3565904A1 (en) 2016-12-29 2019-11-13 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
JP7229923B2 (en) 2017-01-06 2023-02-28 エディタス・メディシン、インコーポレイテッド Methods for assessing nuclease cleavage
WO2018132635A1 (en) 2017-01-12 2018-07-19 Massachusetts Institute Of Technology Methods for analyzing t cell receptors and b cell receptors
US20180208975A1 (en) 2017-01-20 2018-07-26 Merck Sharp & Dohme Corp. Assay for simultaneous genomic and proteomic analysis
EP4310183B1 (en) 2017-01-30 2025-07-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
EP3583214B1 (en) 2017-02-02 2023-11-22 New York Genome Center, Inc. Methods and compositions for identifying or quantifying targets in a biological sample
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US10347365B2 (en) 2017-02-08 2019-07-09 10X Genomics, Inc. Systems and methods for visualizing a pattern in a dataset
GB201704402D0 (en) 2017-03-20 2017-05-03 Blacktrace Holdings Ltd Single cell DNA sequencing
US20230183791A1 (en) 2017-03-24 2023-06-15 National University Of Singapore Methods For Multiplex Detection Of Molecules
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
WO2018191701A1 (en) 2017-04-14 2018-10-18 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
US20180312822A1 (en) 2017-04-26 2018-11-01 10X Genomics, Inc. Mmlv reverse transcriptase variants
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4435113A1 (en) 2017-05-18 2024-09-25 10x Genomics, Inc. Methods and systems for sorting droplets and beads
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
US11530436B2 (en) 2017-05-23 2022-12-20 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018218226A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
WO2018226546A1 (en) 2017-06-05 2018-12-13 10X Genomics, Inc. Gaskets for the distribution of pressures in a microfluidic system
FI3981884T3 (en) 2017-06-07 2023-09-21 Univ Oregon Health & Science Single cell whole genome libraries for methylation sequencing
WO2018236615A1 (en) 2017-06-20 2018-12-27 10X Genomics, Inc. Methods and systems for improved droplet stabilization
WO2019023214A1 (en) 2017-07-25 2019-01-31 Massachusetts Institute Of Technology In situ atac sequencing
EP4488382A3 (en) 2017-08-01 2025-01-22 Illumina, Inc. Hydrogel beads for nucleotide sequencing
US9946577B1 (en) 2017-08-14 2018-04-17 10X Genomics, Inc. Systems and methods for distributed resource management
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
WO2019060907A1 (en) 2017-09-25 2019-03-28 Fred Hutchinson Cancer Research Center High efficiency targeted in situ genome-wide profiling
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019071039A1 (en) 2017-10-04 2019-04-11 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US20190127731A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods for preparing nucleic acid molecules
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Microfluidic channel networks for partitioning
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
WO2019084165A1 (en) 2017-10-27 2019-05-02 10X Genomics, Inc. Methods and systems for sample preparation and analysis
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
CN111699388B (en) 2017-12-12 2024-08-02 10X基因组学有限公司 Systems and methods for single cell processing
CN111712579B (en) 2017-12-22 2024-10-15 10X基因组学有限公司 Systems and methods for processing nucleic acid molecules from one or more cells
CN108173158B (en) 2018-01-05 2023-09-08 浙江正泰电器股份有限公司 Drawer type circuit breaker
WO2019148042A1 (en) 2018-01-26 2019-08-01 10X Genomics, Inc. Compositions and methods for sample processing
EP3749740B1 (en) 2018-02-05 2023-08-30 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
CN112005115A (en) 2018-02-12 2020-11-27 10X基因组学有限公司 Methods to characterize multiple analytes from single cells or cell populations
WO2019165318A1 (en) 2018-02-22 2019-08-29 10X Genomics, Inc. Ligation mediated analysis of nucleic acids
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
WO2019169347A1 (en) 2018-03-02 2019-09-06 10X Genomics, Inc. Systems and apparatus for holding plates
WO2019173638A1 (en) 2018-03-08 2019-09-12 California Institute Of Technology Sample multiplexing for single-cell rna sequencing
WO2019191321A1 (en) 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
EP3775271B1 (en) 2018-04-06 2025-03-12 10X Genomics, Inc. Systems and methods for quality control in single cell processing
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20190345636A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US20190352717A1 (en) 2018-05-18 2019-11-21 10X Genomics, Inc. Targeted non-invasive prenatal testing
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020006183A1 (en) 2018-06-28 2020-01-02 10X Genomics, Inc. Systems and methods for visualization of single-cell resolution characteristics
WO2020010261A1 (en) 2018-07-06 2020-01-09 The Regents Of The University Of California Peptide deficient-mhc class i/chaperone compositions and methods
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US20200033366A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
EP3830289A1 (en) 2018-08-03 2021-06-09 10X Genomics, Inc. Methods and systems to minimize barcode exchange
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
US20200105373A1 (en) 2018-09-28 2020-04-02 10X Genomics, Inc. Systems and methods for cellular analysis using nucleic acid sequencing
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020142779A1 (en) 2019-01-06 2020-07-09 10X Genomics, Inc. Methods and systems for enrichment of barcodes
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
EP3924505A1 (en) 2019-02-12 2021-12-22 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
SG11202110592QA (en) 2019-03-27 2021-10-28 10X Genomics Inc Systems and methods for processing rna from cells
WO2021046475A1 (en) 2019-09-06 2021-03-11 10X Genomics, Inc. Systems and methods for barcoding cells and cell beads
WO2021133845A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US20210270703A1 (en) 2020-02-28 2021-09-02 10X Genomics, Inc. Method for isolating nuclei and cells from tissues
WO2021207610A1 (en) 2020-04-10 2021-10-14 10X Genomics, Inc. Cold protease treatment method for preparing biological samples
WO2021212042A1 (en) 2020-04-16 2021-10-21 10X Genomics, Inc. Compositions and methods for use with fixed samples
WO2021222301A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for analysis and identification of barcode multiplets
WO2021222302A1 (en) 2020-04-27 2021-11-04 10X Genomics, Inc. Methods and systems for increasing cell recovery efficiency
WO2022103712A1 (en) 2020-11-13 2022-05-19 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
EP4298239A1 (en) 2021-02-23 2024-01-03 10X Genomics, Inc. Drug screening methods
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022271908A1 (en) 2021-06-23 2022-12-29 10X Genomics, Inc. Chop-fix method and chopping device for preparing biological samples
WO2023076528A2 (en) 2021-10-28 2023-05-04 10X Genomics, Inc. Methods for sample preparation
WO2024006392A1 (en) 2022-06-29 2024-01-04 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins

Also Published As

Publication number Publication date
EP3752832A1 (en) 2020-12-23
US10725027B2 (en) 2020-07-28
US20240401240A1 (en) 2024-12-05
US20210270818A1 (en) 2021-09-02
US20190383804A1 (en) 2019-12-19
WO2019157529A1 (en) 2019-08-15
US20190367982A1 (en) 2019-12-05
US10928386B2 (en) 2021-02-23
US20190383798A1 (en) 2019-12-19
US20210102936A1 (en) 2021-04-08
US11739440B2 (en) 2023-08-29
US20190367969A1 (en) 2019-12-05
US20190361010A1 (en) 2019-11-28
US12049712B2 (en) 2024-07-30
US10816543B2 (en) 2020-10-27
US11131664B2 (en) 2021-09-28
US20190367966A1 (en) 2019-12-05
US11002731B2 (en) 2021-05-11
US11255847B2 (en) 2022-02-22
CN112005115A (en) 2020-11-27
US20210372998A1 (en) 2021-12-02

Similar Documents

Publication Publication Date Title
SG11202007686VA (en) Methods characterizing multiple analytes from individual cells or cell populations
IL276600A (en) Methods for manufacturing t cells
IL271441A (en) Flow cells
IL289124A (en) Flow cells
SG11202012752RA (en) Flow cells
IL290245A (en) Single cell analysis
IL249617A0 (en) Methods of analyzing nucleic acids from individual cells or cell populations
GB201819540D0 (en) T cell modification
IL287440A (en) Antigen specific cd19-targeted car-t cells
LT3619319T (en) Single cell analysis
SG11202012756UA (en) Flow cells
GB2577258B (en) Battery cell tray
GB201900893D0 (en) Cell contamination assay
IL314599A (en) Cell culture methods
SG11202012394XA (en) Flow cells
GB201904971D0 (en) Cell
EP3890045A4 (en) Electrochemical cell
CA185441S (en) Cell culture insert
GB201906202D0 (en) Cell
EP3864171A4 (en) Cell analysis systems
GB201816520D0 (en) Methods and reagent for analysing nucleic acids from individual cells
GB2570892B (en) Electrochemical cell stack
EP3821035A4 (en) Methods for analyzing cells
GB202209363D0 (en) Battery cell tray
HK40117534A (en) Single cell analysis