ATE391173T1 - Thermostabile ligase aus thermus phage - Google Patents

Thermostabile ligase aus thermus phage

Info

Publication number
ATE391173T1
ATE391173T1 AT03797506T AT03797506T ATE391173T1 AT E391173 T1 ATE391173 T1 AT E391173T1 AT 03797506 T AT03797506 T AT 03797506T AT 03797506 T AT03797506 T AT 03797506T AT E391173 T1 ATE391173 T1 AT E391173T1
Authority
AT
Austria
Prior art keywords
thermostable ligase
thermus
phage
nucleic acids
thermus phage
Prior art date
Application number
AT03797506T
Other languages
English (en)
Inventor
Sigridur Hjorleifsdottir
Audur Thorisdottir
Arnthor Aevarsson
Gudmundur Hreggvidsson
Thorarinn Blondal
Original Assignee
Prokaria Ehf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ehf filed Critical Prokaria Ehf
Application granted granted Critical
Publication of ATE391173T1 publication Critical patent/ATE391173T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
AT03797506T 2002-09-20 2003-09-19 Thermostabile ligase aus thermus phage ATE391173T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US41246102P 2002-09-20 2002-09-20

Publications (1)

Publication Number Publication Date
ATE391173T1 true ATE391173T1 (de) 2008-04-15

Family

ID=32030871

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03797506T ATE391173T1 (de) 2002-09-20 2003-09-19 Thermostabile ligase aus thermus phage

Country Status (6)

Country Link
US (1) US7303901B2 (de)
EP (1) EP1546313B1 (de)
AT (1) ATE391173T1 (de)
AU (1) AU2003260946A1 (de)
DE (1) DE60320119D1 (de)
WO (1) WO2004027054A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2007010571A (es) * 2005-03-04 2008-03-11 Blaze Venture Technologies Ltd Metodo y dispositivo para muestreo bacteriano.
WO2009009139A2 (en) * 2007-07-11 2009-01-15 The General Hospital Corporation Rna ligase polypeptides and methods of selection and use thereof
EP2283132B1 (de) 2008-05-02 2016-10-26 Epicentre Technologies Corporation Selektives 5 -ligations-tagging von rna
CN102076847A (zh) 2008-05-02 2011-05-25 Epi中心科技公司 Rna多磷酸酶组合物、试剂盒及其应用
EP2508529B1 (de) 2008-10-24 2013-08-28 Epicentre Technologies Corporation Transposonendenzusammensetzungen und Verfahren zur Modifizierung von Nukleinsäuren
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP2012517813A (ja) * 2009-02-16 2012-08-09 エピセンター テクノロジーズ コーポレイション １本鎖ｄｎａの鋳型非依存性ライゲーション
WO2011056866A2 (en) 2009-11-05 2011-05-12 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of rna
US20130143276A1 (en) * 2010-04-01 2013-06-06 New England Biolabs, Inc. Compositions and Methods for Adenylating Oligonucleotides
IN2013MN00522A (de) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
DK2665833T3 (en) 2011-01-17 2017-07-24 Life Technologies Corp WORKING PROCEDURE FOR DETECTING LIGANDS USING NUCLEIC ACIDS
WO2015121236A1 (en) * 2014-02-11 2015-08-20 F. Hoffmann-La Roche Ag Targeted sequencing and uid filtering
EP3303377B1 (de) 2015-06-05 2022-11-23 Dana-Farber Cancer Institute, Inc. Zusammensetzungen und verfahren für vorübergehende gentherapie mit verbesserter stabilität
CN109477127A (zh) * 2016-03-14 2019-03-15 R基因股份有限公司 超热稳定的赖氨酸-突变型ssDNA/RNA连接酶
WO2017210469A2 (en) 2016-06-01 2017-12-07 F. Hoffman-La Roche Ag Immuno-pete
CN107557355A (zh) 2016-07-01 2018-01-09 Pgi股份有限公司 建构环状模板和检测dna分子的方法
US11091791B2 (en) 2017-02-24 2021-08-17 Mgi Tech Co., Ltd. Methods for hybridization based hook ligation
EP3610035A4 (de) 2017-04-14 2021-06-09 Dana-Farber Cancer Institute, Inc. Zusammensetzungen und verfahren für vorübergehende gentherapie mit verbesserter stabilität
CN111278974A (zh) 2017-11-09 2020-06-12 深圳华大智造科技有限公司 钩状探针、核酸连接方法以及测序文库的构建方法
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
WO2022015461A1 (en) 2020-07-17 2022-01-20 Qiagen Beverly Llc Thermostable ligase with reduced sequence bias
CN114836401A (zh) * 2022-04-24 2022-08-02 中国人民解放军海军军医大学 重组ssDNA核酸环化酶、制备方法及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US6492161B1 (en) * 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
JP4310599B2 (ja) * 2000-07-05 2009-08-12 東洋紡績株式会社 塩基多型を検出する方法

Also Published As

Publication number Publication date
EP1546313B1 (de) 2008-04-02
US20050266439A1 (en) 2005-12-01
DE60320119D1 (en) 2008-05-15
WO2004027054A1 (en) 2004-04-01
EP1546313A1 (de) 2005-06-29
US7303901B2 (en) 2007-12-04
AU2003260946A1 (en) 2004-04-08

Similar Documents

Publication Publication Date Title
ATE391173T1 (de) Thermostabile ligase aus thermus phage
DE69839557D1 (de) Klonierte genome von infektiösen hepatitis c viren und deren verwendungen
ATE469911T1 (de) Isolierte nukleinsäuremoleküle die t-zellen induzierbare faktoren kodieren (tif), kodierte proteine und ihre anwendung
BR0101493A (pt) Transcrição reversa a altas temperaturasusando polimerases de dna mutantes
WO2003054152A3 (en) Novel nucleic acids and polypeptides
WO2003025148A3 (en) Novel nucleic acids and polypeptides
WO2002070539A3 (en) Novel nucleic acids and polypeptides
WO2003023013A3 (en) Novel nucleic acids and polypeptides
WO2002081731A3 (en) Novel nucleic acids and polypeptides
WO2001075067A3 (en) Novel nucleic acids and polypeptides
WO2001057188A3 (en) Novel nucleic acids and polypeptides
WO2001066689A3 (en) Novel nucleic acids and polypeptides
WO2001064835A8 (en) Novel nucleic acids and polypeptides
WO2003029271A3 (en) Novel nucleic acids and polypeptides
BRPI0403221A (pt) Fragmento de taq polimerase termoestável
EA200300880A1 (ru) Способ детекции нуклеотидного полиморфизма
WO2001064834A8 (en) Novel nucleic acids and polypeptides
WO2004087874A3 (en) Novel nucleic acids and polypeptides
WO2002054775A3 (en) Method for providing an extension code for a binary description for multimedia data
DK1379695T3 (da) Nye polynukleotider og polypeptider af IFNalfa-21-genet
WO2002018424A3 (en) Nucleic acids and polypeptides
ATE309356T1 (de) Konstruierte fluoreszenzproteine mit langen wellenlängen
WO2001079449A3 (en) Novel nucleic acids and polypeptides
DE60034884D1 (de) Klonierte genome von infektiösen hepatitis c viren genotyp 2a und deren verwendungen
ATE340856T1 (de) Mit schizophrenie zusammenhängendes gen und protein

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties