BR0016409A - Processo para avaliar a sensibilidade do hiv a drogas - Google Patents

Processo para avaliar a sensibilidade do hiv a drogas

Info

Publication number
BR0016409A
BR0016409A BR0016409-7A BR0016409A BR0016409A BR 0016409 A BR0016409 A BR 0016409A BR 0016409 A BR0016409 A BR 0016409A BR 0016409 A BR0016409 A BR 0016409A
Authority
BR
Brazil
Prior art keywords
hiv
coding sequence
protease
recombinant
molecular clone
Prior art date
Application number
BR0016409-7A
Other languages
English (en)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of BR0016409A publication Critical patent/BR0016409A/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G01N2333/161—HIV-1, HIV-2 gag-pol, e.g. p55, p24/25, p17/18, p.7, p6, p66/68, p51/52, p31/34, p32, p40

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Physiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"PROCESSO PARA AVALIAR A SENSIBILIDADE DO HIV A DROGAS". é descrito um processo para avaliar a sensibilidade de uma variação de HIV proveniente de uma amostra biológica a pelo menos uma droga capaz de inibir a protease do HIV. O processo compreende as etapas de: a) amplificação do ácido nucléico que codifica a protease do HIV proveniente da amostra biológica, para se obter uma seq³ência codificadora da protease do HIV amplificada; b) clonagem da seq³ência codificadora da protease do HIV amplificada em um sítio de restrição único de um clone molecular modificado de HIV, em que o dito clone molecular modificado de HIV é modificado pelo menos através da deleção da seq³ência codificadora da protease e pela substituição da mesma pelo sítio de restrição único, para se obter um clone molecular recombinante de HIV que compreende e expressa a seq³ência codificadora da protease do HIV; c) introdução do clone molecular recombinante do HIV que compreende e expressa a seq³ência codificadora da protease do HIV em células cultivadas, para se obter células transfectadas com o HIV recombinante; d) medida do nível do antígeno p24 do HIV no sobrenadante das células transfectadas com o HIV recombinante tanto na presença quanto na ausência da droga capaz de inibir a protease do HIV. O processo é adequado para qualquer variação de HIV, para variações de HIV-1 ou de HIV-2. São também descrito kits para diagnóstico.
BR0016409-7A 1999-12-15 2000-12-14 Processo para avaliar a sensibilidade do hiv a drogas BR0016409A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99830776A EP1109019A1 (en) 1999-12-15 1999-12-15 Method to evaluate the sensitivity of HIV variants to drugs able to inhibit the HIV protease
PCT/IT2000/000518 WO2001044508A2 (en) 1999-12-15 2000-12-14 Method to evaluate the sensitivity of hiv variants to drugs able to inhibit the hiv protease

Publications (1)

Publication Number Publication Date
BR0016409A true BR0016409A (pt) 2002-08-20

Family

ID=8243712

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0016409-7A BR0016409A (pt) 1999-12-15 2000-12-14 Processo para avaliar a sensibilidade do hiv a drogas

Country Status (9)

Country Link
US (1) US20030175690A1 (pt)
EP (2) EP1109019A1 (pt)
AT (1) ATE265043T1 (pt)
AU (1) AU2395101A (pt)
BR (1) BR0016409A (pt)
CA (1) CA2393620A1 (pt)
DE (1) DE60010127D1 (pt)
RU (1) RU2002118706A (pt)
WO (1) WO2001044508A2 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0009374D0 (en) * 2000-04-14 2000-05-31 Glaxo Group Ltd Phenotyping assay and reagents therefor
AU2003246119B2 (en) * 2002-06-28 2008-02-21 Fuso Pharmaceutical Industries, Ltd. Anti-HIV agent

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571666A (en) * 1988-10-28 1996-11-05 Oklahoma Medical Research Foundation Thiazine dyes used to inactivate HIV in biological fluids
ES2177922T3 (es) * 1996-01-26 2002-12-16 Virco Bvba Metodo de valoracion la quimioterapia de pacientes hiv-positivos basado en la sensibilidad fenotipica a los farmacos de las cepas de hiv delpaciente.

Also Published As

Publication number Publication date
WO2001044508A3 (en) 2002-01-03
RU2002118706A (ru) 2004-02-27
EP1242818B1 (en) 2004-04-21
EP1242818A2 (en) 2002-09-25
ATE265043T1 (de) 2004-05-15
AU2395101A (en) 2001-06-25
CA2393620A1 (en) 2001-06-21
US20030175690A1 (en) 2003-09-18
EP1109019A1 (en) 2001-06-20
WO2001044508A2 (en) 2001-06-21
DE60010127D1 (de) 2004-05-27

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]