BR112017025929A2 - genes pesticidas e métodos de uso - Google Patents

genes pesticidas e métodos de uso

Info

Publication number
BR112017025929A2
BR112017025929A2 BR112017025929A BR112017025929A BR112017025929A2 BR 112017025929 A2 BR112017025929 A2 BR 112017025929A2 BR 112017025929 A BR112017025929 A BR 112017025929A BR 112017025929 A BR112017025929 A BR 112017025929A BR 112017025929 A2 BR112017025929 A2 BR 112017025929A2
Authority
BR
Brazil
Prior art keywords
polypeptides
methods
compositions
pesticidal
vectors
Prior art date
Application number
BR112017025929A
Other languages
English (en)
Inventor
Parks Jessica
Bulazel Roberts Kira
E Thayer Rebecca
Original Assignee
Agbiome Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agbiome Inc filed Critical Agbiome Inc
Publication of BR112017025929A2 publication Critical patent/BR112017025929A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

são fornecidas composições com atividade pesticida e métodos para sua utilização. as composições incluem polipeptídeos isolados e recombinantes que possuem atividade pesticida, moléculas de ácido nucleico recombinantes e sintéticas que codificam os polipeptídeos, construtos de dna e vetores que compreendem as moléculas de ácido nucleico, células hospedeiras que compreendem os vetores, bem como anticorpos para os polipeptídeos. as sequências de nucleotídeos que codificam os polipeptídeos podem ser utilizadas em construtos de dna ou em cassetes de expressão para transformação e expressão em organismos de interesse. as composições e os métodos fornecidos são úteis para a produção de organismos com maior resistência ou tolerância a pragas. também são fornecidas plantas e sementes transgênicas que compreendem uma sequência de nucleotídeos que codifica uma proteína pesticida da invenção. essas plantas são resistentes a insetos e a outras pragas. são fornecidos métodos para produzir os diversos polipeptídeos aqui descritos e para utilizar esses polipeptídeos para controlar ou matar uma praga. também são incluídos métodos e kits para detectar polipeptídeos da invenção em uma amostra.
BR112017025929A 2015-06-03 2016-05-18 genes pesticidas e métodos de uso BR112017025929A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562170345P 2015-06-03 2015-06-03
PCT/US2016/033126 WO2016196013A2 (en) 2015-06-03 2016-05-18 Pesticidal genes and methods of use

Publications (1)

Publication Number Publication Date
BR112017025929A2 true BR112017025929A2 (pt) 2018-08-14

Family

ID=56404283

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017025929A BR112017025929A2 (pt) 2015-06-03 2016-05-18 genes pesticidas e métodos de uso

Country Status (8)

Country Link
US (4) US10364439B2 (pt)
EP (2) EP3303595A2 (pt)
CN (2) CN114805503A (pt)
AR (1) AR104867A1 (pt)
BR (1) BR112017025929A2 (pt)
CA (1) CA2987598A1 (pt)
MA (2) MA55776A (pt)
WO (1) WO2016196013A2 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2986575A1 (en) 2015-05-21 2016-11-24 Harpoon Therapeutics, Inc. Trispecific binding proteins and methods of use
US10036037B2 (en) * 2015-08-18 2018-07-31 Monsanto Technology Llc Insect inhibitory proteins
IL263102B2 (en) 2016-05-20 2023-11-01 Harpoon Therapeutics Inc A serum albumin-binding protein with a single site
US11623958B2 (en) 2016-05-20 2023-04-11 Harpoon Therapeutics, Inc. Single chain variable fragment CD3 binding proteins
MX2019006043A (es) 2016-11-23 2019-09-26 Harpoon Therapeutics Inc Proteína de unión de antígeno prostático específico de membrana.
WO2018098356A1 (en) 2016-11-23 2018-05-31 Harpoon Therapeutics, Inc. Psma targeting trispecific proteins and methods of use
CN110506117A (zh) * 2017-01-30 2019-11-26 农业生物群落股份有限公司 杀虫基因及使用方法
EP3589662A4 (en) 2017-02-28 2020-12-30 Harpoon Therapeutics, Inc. INDUCTIBLE MONOVALENT ANTIGBINDING PROTEIN
CN110891974B (zh) 2017-05-12 2021-08-06 哈普恩治疗公司 间皮素结合蛋白质
CN115028727A (zh) 2017-05-12 2022-09-09 哈普恩治疗公司 靶向msln的三特异性蛋白质及使用方法
US10927180B2 (en) 2017-10-13 2021-02-23 Harpoon Therapeutics, Inc. B cell maturation antigen binding proteins
US11136403B2 (en) 2017-10-13 2021-10-05 Harpoon Therapeutics, Inc. Trispecific proteins and methods of use
US20220017626A1 (en) * 2018-09-21 2022-01-20 Harpoon Therapeutics, Inc. Egfr binding proteins and methods of use
MX2021003554A (es) 2018-09-25 2021-05-27 Harpoon Therapeutics Inc Proteinas de union a dll3 y metodos de uso.
WO2021168303A1 (en) 2020-02-21 2021-08-26 Harpoon Therapeutics, Inc. Flt3 binding proteins and methods of use
EP4251755A2 (en) 2020-11-24 2023-10-04 AgBiome, Inc. Pesticidal genes and methods of use

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4196265A (en) 1977-06-15 1980-04-01 The Wistar Institute Method of producing antibodies
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US4853331A (en) 1985-08-16 1989-08-01 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5039523A (en) 1988-10-27 1991-08-13 Mycogen Corporation Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5240842A (en) 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
ATE225853T1 (de) 1990-04-12 2002-10-15 Syngenta Participations Ag Gewebe-spezifische promotoren
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
CA2051562C (en) 1990-10-12 2003-12-02 Jewel M. Payne Bacillus thuringiensis isolates active against dipteran pests
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
DE69230290T2 (de) 1991-08-27 2000-07-20 Novartis Ag Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
ZA927576B (en) 1991-10-04 1993-04-16 Univ North Carolina State Pathogen-resistant transgenic plants.
TW261517B (pt) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
US5743477A (en) 1992-08-27 1998-04-28 Dowelanco Insecticidal proteins and method for plant protection
AU684068B2 (en) 1993-03-25 1997-12-04 Syngenta Participations Ag Novel pesticidal proteins and strains
US5849870A (en) 1993-03-25 1998-12-15 Novartis Finance Corporation Pesticidal proteins and strains
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US5723440A (en) * 1995-06-07 1998-03-03 Mycogen Corporation Controlling hemipteran insect pests with Bacillus thuringiensis
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US6063756A (en) 1996-09-24 2000-05-16 Monsanto Company Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
KR20010006015A (ko) 1997-04-03 2001-01-15 한스 루돌프 하우스, 헨리테 브룬너, 베아트리체 귄터 식물 해충 방제 방법
CA2315546C (en) 1998-02-26 2008-04-29 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
EP1056862A1 (en) 1998-02-26 2000-12-06 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
AU5489099A (en) 1998-08-20 2000-03-14 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
US6468523B1 (en) 1998-11-02 2002-10-22 Monsanto Technology Llc Polypeptide compositions toxic to diabrotic insects, and methods of use
CA2371442A1 (en) 1999-05-04 2000-11-09 Monsanto Technology Llc Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants
US6593293B1 (en) 1999-09-15 2003-07-15 Monsanto Technology, Llc Lepidopteran-active Bacillus thuringiensis δ-endotoxin compositions and methods of use
BR0016030A (pt) 1999-11-29 2002-07-30 Midwest Oilseeds Inc Métodos e composições para a introdução de moléculas em células
US20050183161A1 (en) 2003-10-14 2005-08-18 Athenix Corporation AXMI-010, a delta-endotoxin gene and methods for its use
WO2005066202A2 (en) 2003-12-22 2005-07-21 E.I. Du Pont De Nemours And Company Bacillus cry9 family members
US7473822B1 (en) 2005-06-27 2009-01-06 Iowa State University Research Foundation, Inc. Soybean transformation and regeneration using half-seed explant
BRPI0713646B1 (pt) 2006-06-14 2017-11-21 Athenix Corporation Nucleic acid molecule; vector; hospedeira cell; polipeptídeo with pesticide activity and method for their production; composition; method for obtaining a plant, as well as methods for control of pest populations; to exterminate a plague of lepidópteros or coleópteros; to protect a plant of a nematode prague
EP3150625B1 (en) 2006-06-15 2021-01-27 BASF Agricultural Solutions Seed US LLC A family of pesticidal proteins and methods for their use
WO2009099602A1 (en) 2008-02-04 2009-08-13 Massachusetts Institute Of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
EP2297189A2 (en) * 2008-06-25 2011-03-23 Athenix Corporation Toxin genes and methods for their use
CA3183317A1 (en) * 2008-07-02 2010-01-07 BASF Agricultural Solutions Seed US LLC Axmi-115, axmi-113, axmi-005, axmi-163 and axmi-184: insecticidal proteins and methods for their use
AR075634A1 (es) * 2009-02-27 2011-04-20 Athenix Corp Proteinas pesticidas y metodos de uso de las mismas
RS55550B1 (sr) 2009-07-02 2017-05-31 Athenix Corp Axmi-205 pesticidni gen i postupci za njegovu upotrebu
WO2011084324A2 (en) 2009-12-21 2011-07-14 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with lepidopteran activity
CA2785195A1 (en) * 2009-12-21 2011-07-14 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with coleopteran activity
CN103154247A (zh) 2010-08-19 2013-06-12 先锋国际良种公司 对鳞翅目昆虫具有活性的新苏云金杆菌基因
US8878007B2 (en) 2011-03-10 2014-11-04 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
EP2744920A4 (en) * 2011-08-19 2015-06-03 Synthetic Genomics Inc INTEGRATED METHOD FOR HIGH-BY-PACK IDENTIFICATION OF NEW PESTICIDE COMPOSITIONS AND THEIR USE
CN103890181A (zh) 2011-08-22 2014-06-25 拜尔作物科学公司 修饰植物基因组的方法和手段
CA3181424A1 (en) * 2012-03-09 2013-09-12 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
EP3076991A2 (en) * 2013-09-25 2016-10-12 Axcella Health Inc. Nutritive polypeptides and formulations thereof, and methods of production and use thereof

Also Published As

Publication number Publication date
CA2987598A1 (en) 2016-12-08
EP3303595A2 (en) 2018-04-11
MA46313A (fr) 2021-04-28
US20210054403A1 (en) 2021-02-25
WO2016196013A3 (en) 2017-03-16
US20160355842A1 (en) 2016-12-08
US20180163223A1 (en) 2018-06-14
CN114805503A (zh) 2022-07-29
MA55776A (fr) 2022-03-02
EP3960864A2 (en) 2022-03-02
WO2016196013A2 (en) 2016-12-08
US10421976B2 (en) 2019-09-24
CN108271390A (zh) 2018-07-10
EP3960864A3 (en) 2022-06-15
US10364439B2 (en) 2019-07-30
AR104867A1 (es) 2017-08-23
US20190284571A1 (en) 2019-09-19

Similar Documents

Publication Publication Date Title
CL2020001838A1 (es) Genes pesticidas y métodos de uso. (divisional solicitud 201702681)
BR112017025929A2 (pt) genes pesticidas e métodos de uso
BR112018012893A2 (pt) genes pesticidas e métodos de uso
BR112019004406A2 (pt) genes pesticidas e métodos de uso
WO2018140859A3 (en) Pesticidal genes and methods of use
PH12021550691A1 (en) Pesticidal genes and methods of use
BR112017022383A2 (pt) polipeptídeo recombinante, composição, molécula de ácido nucleico recombinante, ácido nucleico recombinante, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo, planta, semente transgênica da planta, método para proteger uma planta, método para aumentar o rendimento em uma planta
BR112017013633A2 (pt) polipeptídeo recombinante, molécula de ácido nucleico recombinante, composição, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo com atividade pesticida, planta, semente transgênica, método para proteger uma planta de uma praga de insetos, método para aumentar o rendimento em uma planta
AR109512A1 (es) Genes pesticidas y métodos de uso
AR111369A1 (es) Genes pesticidas y métodos de uso
AR113099A1 (es) Genes pesticidas y métodos de uso
AR114390A1 (es) Genes pesticidas y métodos de uso

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.