BR112018007070A2 - método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna - Google Patents

método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna

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Publication number
BR112018007070A2
BR112018007070A2 BR112018007070A BR112018007070A BR112018007070A2 BR 112018007070 A2 BR112018007070 A2 BR 112018007070A2 BR 112018007070 A BR112018007070 A BR 112018007070A BR 112018007070 A BR112018007070 A BR 112018007070A BR 112018007070 A2 BR112018007070 A2 BR 112018007070A2
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Brazil
Prior art keywords
plant
composition
protoplast
kit
preparing
Prior art date
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BR112018007070A
Other languages
English (en)
Inventor
Wook WOO Je
Soo Kim Jin
Kim Jungeun
Il Kwon Soon
Choe Sunghwa
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Aict
Inst Basic Science
Seoul Nat Univ R&Db Foundation
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Application filed by Aict, Inst Basic Science, Seoul Nat Univ R&Db Foundation filed Critical Aict
Publication of BR112018007070A2 publication Critical patent/BR112018007070A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8298—Brassinosteroids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1472—Lactuca sativa [lettuce]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Abstract

método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna, refere-se a invenção a um método para produzir plantas inteiras a partir de protoplastos compreendendo knocking-out ou knocking em um ou mais genes endógenos do protoplasto; a uma planta regenerada a partir de um protoplasto modificado pelo genoma preparado pelo método acima; a uma composição para clivagem de dna que codifica o gene bin2 em uma célula vegetal; a uma composição e um kit para a preparação de uma planta a parir de um protoplasto.
BR112018007070A 2015-10-06 2016-10-06 método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna BR112018007070A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20150140314 2015-10-06
PCT/KR2016/011217 WO2017061806A1 (en) 2015-10-06 2016-10-06 Method for producing whole plants from protoplasts

Publications (1)

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BR112018007070A2 true BR112018007070A2 (pt) 2019-06-04

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BR112018007070A BR112018007070A2 (pt) 2015-10-06 2016-10-06 método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna
BR112018007061A BR112018007061A2 (pt) 2015-10-06 2016-10-06 método e composição para aumentar a eficiência de produção de uma planta editada pelo genoma a partir de um protoplasto vegetal e planta regenerada a partir do mesmo

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US (3) US11136588B2 (pt)
EP (2) EP3366777A4 (pt)
JP (3) JP6916188B2 (pt)
KR (2) KR102107735B1 (pt)
CN (3) CN111961683A (pt)
AU (3) AU2016336565B2 (pt)
BR (2) BR112018007070A2 (pt)
CA (3) CA3080849A1 (pt)
MX (2) MX2018004264A (pt)
WO (2) WO2017061806A1 (pt)

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CA3080849A1 (en) 2017-04-13
CN108368516A (zh) 2018-08-03
CN108473998B (zh) 2020-11-20
JP6855543B2 (ja) 2021-04-07
AU2016336565B2 (en) 2019-07-18
US20210340554A1 (en) 2021-11-04
KR20170041641A (ko) 2017-04-17
CN111961683A (zh) 2020-11-20
CN108473998A (zh) 2018-08-31
KR20170041640A (ko) 2017-04-17
US11136588B2 (en) 2021-10-05
US11149281B2 (en) 2021-10-19
JP2018530348A (ja) 2018-10-18
KR102107735B1 (ko) 2020-05-11
AU2016336565A1 (en) 2018-05-17
CA3001108A1 (en) 2017-04-13
KR101829885B1 (ko) 2018-03-30
AU2019204375A1 (en) 2019-07-11
EP3366777A4 (en) 2019-03-20
MX2018004265A (es) 2018-11-09
JP2020022455A (ja) 2020-02-13
EP3359674A1 (en) 2018-08-15
JP2018530350A (ja) 2018-10-18
AU2019204375B2 (en) 2021-05-27
US20180312869A1 (en) 2018-11-01
JP6885954B2 (ja) 2021-06-16
MX2018004264A (es) 2018-11-09
AU2016336566A1 (en) 2018-05-24
CA3001232A1 (en) 2017-04-13
WO2017061806A1 (en) 2017-04-13
BR112018007061A2 (pt) 2019-01-15
WO2017061805A1 (ko) 2017-04-13
CA3001232C (en) 2020-07-07
AU2016336566B2 (en) 2020-05-14
US20190338298A1 (en) 2019-11-07
EP3366777A1 (en) 2018-08-29
EP3359674A4 (en) 2019-03-13
JP6916188B2 (ja) 2021-08-11

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