BR112015030491A8 - método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula - Google Patents

método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula Download PDF

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BR112015030491A8
BR112015030491A8 BR112015030491A BR112015030491A BR112015030491A8 BR 112015030491 A8 BR112015030491 A8 BR 112015030491A8 BR 112015030491 A BR112015030491 A BR 112015030491A BR 112015030491 A BR112015030491 A BR 112015030491A BR 112015030491 A8 BR112015030491 A8 BR 112015030491A8
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nucleic acid
cell
expression
modulating
protein
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BR112015030491A
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BR112015030491A2 (pt
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M Church George
M Iwanicki Kevin
G Mali Prashant
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Harvard College
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Abstract

resumo "regulação transcricional guiada por rna" métodos de modulação da expressão de um ácido nucleico alvo em uma célula são fornecidos, incluindo a introdução na célula de um primeiro ácido nucleico exógeno que codifica um ou mais rnas complementares ao dna, em que o dna inclui o ácido nucleico alvo, introdução na célula de um segundo ácido nucleico exógeno que codifica uma proteína cas9 nuclease-null que se liga ao dna e é guiada por um ou mais rnas, introdução na célula de um terceiro ácido nucleico exógeno que codifica uma proteína reguladora da transcrição ou domínio, em que o um ou mais rnas, a proteína cas9 nuclease-null, e a proteína reguladora da transcrição ou domínio são expressos, em que o um ou mais rnas, a proteína cas9 nuclease-null e a proteína reguladora da transcrição ou domínio co-localizam o dna e em que a proteína reguladora da transcrição ou domínio regula a expressão do ácido nucleico alvo.
BR112015030491A 2013-06-04 2014-06-04 método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula BR112015030491A8 (pt)

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US201361830787P 2013-06-04 2013-06-04
PCT/US2014/040868 WO2014197568A2 (en) 2013-06-04 2014-06-04 Rna-guideded transcriptional regulation

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BR112015030491A2 BR112015030491A2 (pt) 2017-07-25
BR112015030491A8 true BR112015030491A8 (pt) 2019-12-24

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US (2) US11981917B2 (pt)
EP (3) EP3603679B1 (pt)
JP (5) JP6621738B2 (pt)
KR (3) KR102512979B1 (pt)
CN (2) CN113846096A (pt)
AU (4) AU2014274939B2 (pt)
BR (1) BR112015030491A8 (pt)
CA (2) CA3176690A1 (pt)
ES (1) ES2930537T3 (pt)
IL (3) IL284773B2 (pt)
MX (2) MX2015016798A (pt)
MY (2) MY177814A (pt)
RU (2) RU2690935C2 (pt)
SG (3) SG11201509962QA (pt)
WO (1) WO2014197568A2 (pt)

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