BR112017017886A2 - gerando populações de células endoteliais arteriais - Google Patents

gerando populações de células endoteliais arteriais

Info

Publication number
BR112017017886A2
BR112017017886A2 BR112017017886A BR112017017886A BR112017017886A2 BR 112017017886 A2 BR112017017886 A2 BR 112017017886A2 BR 112017017886 A BR112017017886 A BR 112017017886A BR 112017017886 A BR112017017886 A BR 112017017886A BR 112017017886 A2 BR112017017886 A2 BR 112017017886A2
Authority
BR
Brazil
Prior art keywords
endothelial cell
cell populations
arterial endothelial
generating
generating arterial
Prior art date
Application number
BR112017017886A
Other languages
English (en)
Other versions
BR112017017886B1 (pt
Inventor
A Thomson James
Zhang Jue
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Publication of BR112017017886A2 publication Critical patent/BR112017017886A2/pt
Publication of BR112017017886B1 publication Critical patent/BR112017017886B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/35—Polyols, e.g. glycerin, inositol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Vascular Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

são descritos métodos para gerar células endoteliais arteriais humanas em condições definidas na ausência de insulina.
BR112017017886-9A 2015-02-20 2016-02-19 Método para obter células endoteliais arteriais humanas, populações isoladas puras de células endoteliais arteriais, método de rastreio de um agente in vitro, método in vitro para vascularizar um construto de tecido engenheirado, uso das referidas células e composição farmacêutica compreendendo as referidas células BR112017017886B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562118553P 2015-02-20 2015-02-20
US62/118,553 2015-02-20
PCT/US2016/018769 WO2016134313A1 (en) 2015-02-20 2016-02-19 Generating arterial endothelial cell populations

Publications (2)

Publication Number Publication Date
BR112017017886A2 true BR112017017886A2 (pt) 2018-04-10
BR112017017886B1 BR112017017886B1 (pt) 2024-02-06

Family

ID=55543054

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017017886-9A BR112017017886B1 (pt) 2015-02-20 2016-02-19 Método para obter células endoteliais arteriais humanas, populações isoladas puras de células endoteliais arteriais, método de rastreio de um agente in vitro, método in vitro para vascularizar um construto de tecido engenheirado, uso das referidas células e composição farmacêutica compreendendo as referidas células

Country Status (10)

Country Link
US (2) US11674123B2 (pt)
EP (1) EP3259344B1 (pt)
JP (2) JP6691921B2 (pt)
CN (1) CN107257852B (pt)
AU (1) AU2016219815B2 (pt)
BR (1) BR112017017886B1 (pt)
CA (1) CA2975268A1 (pt)
IL (1) IL253580B (pt)
SG (1) SG11201706706XA (pt)
WO (1) WO2016134313A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10787640B2 (en) * 2015-03-03 2020-09-29 The Board Of Trustees Of The Leland Stanford Junior University Producing mesodermal cell types and methods of using the same
US20180135021A1 (en) * 2016-11-16 2018-05-17 Allele Biotechnology And Pharmaceuticals, Inc. Induction of pancreatic beta cells by stem cell differentiation with rna
EP3550013A4 (en) 2016-12-02 2020-06-24 Takara Bio Inc. PROCESS FOR PRODUCING ENDOTHELIAL CELLS
CN108048390B (zh) * 2017-12-21 2021-04-27 清华大学 一种制备血管内皮细胞的方法及其专用试剂盒
AU2019333301A1 (en) * 2018-08-31 2021-02-25 Wisconsin Alumni Research Foundation Generating arterial endothelial cell-seeded vascular grafts
WO2020051453A1 (en) 2018-09-07 2020-03-12 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells
GB201911957D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of producing haemogenic progenitor cells from pluripotent stem cells
US20240026301A1 (en) * 2022-07-21 2024-01-25 Trailhead Biosystems Inc. Methods and compositions for generating endothelial cells from pluripotent stem cells
CN115369078A (zh) * 2022-08-19 2022-11-22 创芯国际生物科技(广州)有限公司 软骨类器官诱导培养基及诱导方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6326198B1 (en) 1990-06-14 2001-12-04 Regents Of The University Of Michigan Methods and compositions for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
DE69633668T2 (de) 1995-05-25 2006-03-09 Shimon Slavin Allogene zelltherapie für krebs infolge allogener stammzellen transplantation
WO1997041854A1 (en) * 1996-05-07 1997-11-13 The Trustees Of The University Of Pennsylvania Inhibitors of glycogen synthase kinase-3 and methods for identifying and using the same
WO1998030679A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US5994071A (en) 1997-04-04 1999-11-30 Albany Medical College Assessment of prostate cancer
AU3066599A (en) 1998-03-03 1999-10-18 University Of Southern California Use of cytokines and mitogens to inhibit graft versus host disease
US6383481B1 (en) 1998-03-30 2002-05-07 Japan Immunoresearch Laboratories Co., Ltd. Method for transplantation of hemopoietic stem cells
EP1725230A4 (en) 2004-03-16 2009-08-19 Intherthyr Corp METHIMAZOLE DERIVATIVES AND TAUTOMER CYCLIC THIONES FOR INHIBITING CELL ADHESION
EP2428563A1 (en) 2005-02-10 2012-03-14 Regents Of The University Of Minnesota Vascular/lymphatic endothelial cells
US20090227026A1 (en) 2008-02-14 2009-09-10 Rapoport H Scott Tissue engineering scaffolds
US20120301443A1 (en) * 2009-12-29 2012-11-29 Cornell University Methods for developing endothelial cells from pluripotent cells and endothelial cells derived
US20120064040A1 (en) * 2010-06-03 2012-03-15 The Regents Of The University Of California Serum free culture medium and supplement
US20120295347A1 (en) * 2011-05-20 2012-11-22 Steven Kessler Methods and Compositions for Producing Endothelial Progenitor Cells from Pluripotent Stem Cells
CN104302764A (zh) 2012-05-02 2015-01-21 国际干细胞公司 由人多能干细胞衍生内皮细胞
EP2893031B1 (en) 2012-09-10 2018-06-20 Allegheny-Singer Research Institute Isolation of pulmonary arterial endothelial cells from patients with pulmonary vascular disease and uses thereof
DK2935567T3 (da) * 2012-12-18 2019-05-06 Cnc Centro De Neurociencias E Biologia Molecular Univ De Coimbra Differentieret cellepopulation af endotelceller afledt af humane pluripotente stamceller, sammensætning, system, kit og anvendelser deraf
US20140248696A1 (en) * 2013-03-01 2014-09-04 Wisconsin Alumni Research Foundation Methods of maintaining, expanding, and diffrentiating neuronal subtype specific progenitors
US9188561B2 (en) 2013-03-03 2015-11-17 Yue Xu Test strip
SG11201507275TA (en) 2013-03-13 2015-10-29 Wisconsin Alumni Res Found Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions

Also Published As

Publication number Publication date
IL253580A0 (en) 2017-09-28
EP3259344A1 (en) 2017-12-27
JP6691921B2 (ja) 2020-05-13
US20240026302A1 (en) 2024-01-25
US11674123B2 (en) 2023-06-13
EP3259344B1 (en) 2021-06-30
AU2016219815A1 (en) 2017-08-10
JP2020072722A (ja) 2020-05-14
US20160244719A1 (en) 2016-08-25
BR112017017886B1 (pt) 2024-02-06
WO2016134313A1 (en) 2016-08-25
IL253580B (en) 2021-02-28
CN107257852B (zh) 2021-09-21
CN107257852A (zh) 2017-10-17
SG11201706706XA (en) 2017-09-28
AU2016219815B2 (en) 2022-03-10
CA2975268A1 (en) 2016-08-25
JP2018505685A (ja) 2018-03-01

Similar Documents

Publication Publication Date Title
BR112017017886A2 (pt) gerando populações de células endoteliais arteriais
CY1123142T1 (el) Βελτιωμενες μεθοδοι για την παραγωγη θετων κυτταρικων θεραπειων
CY1123823T1 (el) Βελτιωμενες συνθεσεις τ κυτταρων
CL2018000194A1 (es) Compuestos de piridina
GB2531952A (en) SC-ß cells and compositions and methods for generating the same
EA202090931A2 (ru) Композиции и способы перепрограммирования tcr с помощью гибридных белков
IL249828A0 (en) Limbal stem cells of cultured mammals, methods of their creation and uses thereof
TWD170353S (zh) 項鍊
BR112017007636A2 (pt) métodos para a preparação de ribosídeos
UY34824A (es) Nucleósidos de espirooxetano de uracilo
UY34484A (es) Benzotienilo-pirrolotriazinas disustituidas y sus usos
UY34781A (es) Moduladores de la via del complemento y usos de los mismos
UY34167A (es) Pirrolotriazinas sustituidas con hidroximetilarilo y sus usos
MX2016004802A (es) Anticuerpos anti-r-espondina (anti-rspo) y metodos de uso.
MX363690B (es) Expansión de celulas madre adultas in vitro.
CY1123144T1 (el) Επαγωγη καρδιακης αναγεννησης μe microrna
MX2015010943A (es) Metodos de cultivo por perfusión con microportadores y usos de los mismos.
MX2020009670A (es) Metodos de cultivo por perfusion y usos de los mismos.
MX367025B (es) Metodos de cultivo por perfusion con microportadores y usos de los mismos.
BR112017020513A2 (pt) método de produção de peixe para transplante, peixe para transplante, método de produção de espécie híbrida de peixe e espécie híbrida de peixe
BR112019001409A2 (pt) células renais bioativas para o tratamento de doença renal crônica
EA201890439A1 (ru) ИММУНООНКОЛОГИЧЕСКАЯ МЕЗОДЕРМАЛЬНАЯ КЛЕТКА-ПРЕДШЕСТВЕННИК (ioMP-КЛЕТКА)
IN2014DE01009A (pt)
EP3490674A4 (en) ALPHA SYNUCLEIN IN PERIPHERAL MONONUCLEAR BLOOD CELLS AS A BIOMARKER FOR SYNUCLEINOPATHIES
UY34564A (es) Utilización en terapéutica de derivados de imidazopiridina

Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 19/02/2016, OBSERVADAS AS CONDICOES LEGAIS