MX367025B - Metodos de cultivo por perfusion con microportadores y usos de los mismos. - Google Patents

Metodos de cultivo por perfusion con microportadores y usos de los mismos.

Info

Publication number
MX367025B
MX367025B MX2015010941A MX2015010941A MX367025B MX 367025 B MX367025 B MX 367025B MX 2015010941 A MX2015010941 A MX 2015010941A MX 2015010941 A MX2015010941 A MX 2015010941A MX 367025 B MX367025 B MX 367025B
Authority
MX
Mexico
Prior art keywords
culturing methods
perfusion culturing
microcarrier perfusion
methods
microcarrier
Prior art date
Application number
MX2015010941A
Other languages
English (en)
Other versions
MX2015010941A (es
Inventor
Yang Yang
Yang Jianguo
Tengtrakool Jennifer
Zhou Weichang
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of MX2015010941A publication Critical patent/MX2015010941A/es
Publication of MX367025B publication Critical patent/MX367025B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2531/00—Microcarriers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a métodos de cultivo de una célula de mamífero y varios métodos que utilizan estos métodos de cultivo. Un método para cultivar una célula mamífero adherente, dicho método está caracterizado porque comprende: (a) proporcionar un matraz de agitación que contiene una célula de mamífero adherente dispuesta en un primer medio de cultivo líquido que ocupa de 20% a 30% del volumen del matraz de agitación y contiene una pluralidad de microportadores a una concentración de 1.0g/L a 15.0g/L; (b) incubar el matraz de agitación durante un primer periodo de tiempo a 35°C hasta 39°C con una agitación rotativa de 85 revoluciones por minuto (rpm) a 125 rpm, y después de las primeras 48 a 96 horas del primer periodo de tiempo, en cada periodo de 24 horas posteriores, (i) retirar de manera continua o periódica un primer volumen del primer medio de cultivo líquido que está sustancialmente libre de microportadores del matraz de agitación, en donde el primer volumen es de 10% a 85% del volumen del primer medio de cultivo líquido; y (ii) añadir al matraz de agitación un segundo volumen de un segundo medio de cultivo líquido, en done el primer y segundo volúmenes son iguales; (c) incubar el matraz de agitación después de que la concentración de células alcance la densidad de células diana durante un segundo periodo de tiempo de 2 días a 7 días, de 32°C a 39°C con agitación rotativa, y en cada periodo de 24 horas, realizar las etapas (b) (i) y (b) (ii) , en donde el primer y segundo medio de cultivo líquido utilizados en la etapa (b) son de un tipo sustancialmente diferente de los utilizados en la etapa (c); y (d) incubar el matraz de agitación durante un tercer período de tiempo superior a 2 días de 35°C a 39°C con la agitación rotativa, y en cada período de 24 horas, realizar las etapas (b) (I9 y (b) (ii) , en donde el primero y segundo medio de cultivo líquido utilizados en la etapa (c) son del mismo tipo que los utilizados en la etapa (d).
MX2015010941A 2013-02-22 2014-02-21 Metodos de cultivo por perfusion con microportadores y usos de los mismos. MX367025B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361768085P 2013-02-22 2013-02-22
PCT/US2014/017803 WO2014130872A2 (en) 2013-02-22 2014-02-21 Microcarrier perfusion culturing methods and uses thereof

Publications (2)

Publication Number Publication Date
MX2015010941A MX2015010941A (es) 2016-06-07
MX367025B true MX367025B (es) 2019-08-02

Family

ID=50240051

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015010941A MX367025B (es) 2013-02-22 2014-02-21 Metodos de cultivo por perfusion con microportadores y usos de los mismos.
MX2019009189A MX2019009189A (es) 2013-02-22 2015-08-21 Metodos de cultivo por perfusion con microportadores y usos de los mismos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019009189A MX2019009189A (es) 2013-02-22 2015-08-21 Metodos de cultivo por perfusion con microportadores y usos de los mismos.

Country Status (9)

Country Link
US (3) US9909101B2 (es)
EP (1) EP2958988A2 (es)
CN (1) CN105209603A (es)
AU (1) AU2014218723B2 (es)
CA (1) CA2901950A1 (es)
HK (1) HK1219751A1 (es)
MX (2) MX367025B (es)
SG (2) SG10201806083RA (es)
WO (1) WO2014130872A2 (es)

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Also Published As

Publication number Publication date
CA2901950A1 (en) 2014-08-28
US20200255798A1 (en) 2020-08-13
AU2014218723A1 (en) 2015-09-10
US20160002594A1 (en) 2016-01-07
MX2019009189A (es) 2019-09-26
WO2014130872A3 (en) 2014-10-16
SG11201506343QA (en) 2015-09-29
US9909101B2 (en) 2018-03-06
AU2014218723B2 (en) 2019-12-05
US10577583B2 (en) 2020-03-03
EP2958988A2 (en) 2015-12-30
WO2014130872A2 (en) 2014-08-28
CN105209603A (zh) 2015-12-30
SG10201806083RA (en) 2018-08-30
MX2015010941A (es) 2016-06-07
US20180148684A1 (en) 2018-05-31
HK1219751A1 (zh) 2017-04-13

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