BR112015030491A2 - regulação transcricional guiada por rna - Google Patents

regulação transcricional guiada por rna

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Publication number
BR112015030491A2
BR112015030491A2 BR112015030491A BR112015030491A BR112015030491A2 BR 112015030491 A2 BR112015030491 A2 BR 112015030491A2 BR 112015030491 A BR112015030491 A BR 112015030491A BR 112015030491 A BR112015030491 A BR 112015030491A BR 112015030491 A2 BR112015030491 A2 BR 112015030491A2
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Brazil
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nucleic acid
cell
dna
protein
rna
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BR112015030491A
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English (en)
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BR112015030491A8 (pt
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M Church George
M Iwanicki Kevin
G Mali Prashant
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Harvard College
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Publication of BR112015030491A2 publication Critical patent/BR112015030491A2/pt
Publication of BR112015030491A8 publication Critical patent/BR112015030491A8/pt

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Abstract

resumo "regulação transcricional guiada por rna" métodos de modulação da expressão de um ácido nucleico alvo em uma célula são fornecidos, incluindo a introdução na célula de um primeiro ácido nucleico exógeno que codifica um ou mais rnas complementares ao dna, em que o dna inclui o ácido nucleico alvo, introdução na célula de um segundo ácido nucleico exógeno que codifica uma proteína cas9 nuclease-null que se liga ao dna e é guiada por um ou mais rnas, introdução na célula de um terceiro ácido nucleico exógeno que codifica uma proteína reguladora da transcrição ou domínio, em que o um ou mais rnas, a proteína cas9 nuclease-null, e a proteína reguladora da transcrição ou domínio são expressos, em que o um ou mais rnas, a proteína cas9 nuclease-null e a proteína reguladora da transcrição ou domínio co-localizam o dna e em que a proteína reguladora da transcrição ou domínio regula a expressão do ácido nucleico alvo.
BR112015030491A 2013-06-04 2014-06-04 método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula BR112015030491A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361830787P 2013-06-04 2013-06-04
PCT/US2014/040868 WO2014197568A2 (en) 2013-06-04 2014-06-04 Rna-guideded transcriptional regulation

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BR112015030491A2 true BR112015030491A2 (pt) 2017-07-25
BR112015030491A8 BR112015030491A8 (pt) 2019-12-24

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BR112015030491A BR112015030491A8 (pt) 2013-06-04 2014-06-04 método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula

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US (2) US11981917B2 (pt)
EP (3) EP3003392B1 (pt)
JP (5) JP6621738B2 (pt)
KR (3) KR102512979B1 (pt)
CN (2) CN113846096A (pt)
AU (4) AU2014274939B2 (pt)
BR (1) BR112015030491A8 (pt)
CA (2) CA3176690A1 (pt)
ES (1) ES2930537T3 (pt)
IL (3) IL284773B2 (pt)
MX (2) MX2015016798A (pt)
MY (2) MY177814A (pt)
RU (2) RU2690935C2 (pt)
SG (3) SG10201710030QA (pt)
WO (1) WO2014197568A2 (pt)

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