DE60140317D1 - Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden - Google Patents

Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden

Info

Publication number
DE60140317D1
DE60140317D1 DE60140317T DE60140317T DE60140317D1 DE 60140317 D1 DE60140317 D1 DE 60140317D1 DE 60140317 T DE60140317 T DE 60140317T DE 60140317 T DE60140317 T DE 60140317T DE 60140317 D1 DE60140317 D1 DE 60140317D1
Authority
DE
Germany
Prior art keywords
polynucleotides
kits
improving
hybridization rates
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60140317T
Other languages
English (en)
Inventor
Michael M Becker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of DE60140317D1 publication Critical patent/DE60140317D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE60140317T 2000-12-14 2001-12-07 Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden Expired - Lifetime DE60140317D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25553500P 2000-12-14 2000-12-14
PCT/US2001/048592 WO2002048404A2 (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides

Publications (1)

Publication Number Publication Date
DE60140317D1 true DE60140317D1 (de) 2009-12-10

Family

ID=22968766

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60140317T Expired - Lifetime DE60140317D1 (de) 2000-12-14 2001-12-07 Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden

Country Status (8)

Country Link
US (2) US7374945B2 (de)
EP (1) EP1377679B1 (de)
JP (1) JP2004537263A (de)
AT (1) ATE447043T1 (de)
AU (2) AU2002230901B2 (de)
CA (1) CA2436857A1 (de)
DE (1) DE60140317D1 (de)
WO (1) WO2002048404A2 (de)

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DE60228100D1 (de) * 2001-08-24 2008-09-18 Nof Corp Verfahren zur beurteilung einer fehlpaarung zwischen einzelsträngigen nukleinsäuremolekülen
US6919180B2 (en) * 2002-03-22 2005-07-19 Sigma Genosys, L.P. Hybridization rate enhancement for substrate-bound specific nucleic acid-binding agents
DE10237284A1 (de) * 2002-08-14 2004-03-04 Advalytix Ag Nukleinsäurenachweis
JP4874801B2 (ja) * 2003-06-11 2012-02-15 イデラ ファーマシューティカルズ インコーポレイテッド 安定化免疫調節オリゴヌクレオチド
ATE476528T1 (de) * 2004-12-08 2010-08-15 Gen Probe Inc Nukleinsäuredetektion aus verschiedenen typen des humanen papillomavirus
ES2381279T3 (es) * 2005-05-06 2012-05-24 Gen-Probe Incorporated Métodos y productos para la captura de ácido nucleico diana
US7488581B2 (en) * 2006-03-20 2009-02-10 Kabushiki Kaisha Toshiba Method for detecting a target nucleic acid sequence
CN102084004B (zh) 2008-05-27 2016-01-20 丹麦达科有限公司 杂交组合物和方法
WO2010097656A1 (en) 2009-02-26 2010-09-02 Dako Denmark A/S Compositions and methods for performing a stringent wash step in hybridization applications
EP2761028A1 (de) 2011-09-30 2014-08-06 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren mit formamid
EP2766498B1 (de) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
EP3252173A1 (de) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
EP4249605A3 (de) 2011-12-22 2023-11-15 President And Fellows Of Harvard College Verfahren zum nachweis von analyten
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
EP3578666A1 (de) 2013-03-12 2019-12-11 President and Fellows of Harvard College Verfahren zur erzeugung einer dreidimensionalen nukleinsäure mit matrix
MY177814A (en) 2013-06-04 2020-09-23 Harvard College Rna-guided transcriptional regulation
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
WO2017189525A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
WO2018045186A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
EP3507364A4 (de) * 2016-08-31 2020-05-20 President and Fellows of Harvard College Verfahren zur erstellung von bibliotheken von nukleinsäuresequenzen zur detektion mittels in-situ-fluoreszenzsequenzierung
CN109789167A (zh) 2017-04-14 2019-05-21 哈佛学院董事及会员团体 用于产生细胞衍生的微丝网络的方法
CN116236282A (zh) 2017-05-05 2023-06-09 史赛克欧洲运营有限公司 手术导航系统
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
AU2019314449A1 (en) * 2018-08-01 2021-03-11 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of Epstein-Barr virus
CN109988842B (zh) * 2019-04-19 2022-12-06 上海吉玛制药技术有限公司 一种寡核苷酸标记探针原位检测pdl1的试剂组与应用
WO2023106200A1 (ja) * 2021-12-10 2023-06-15 日東紡績株式会社 核酸の対象塩基配列中における変異を検出するための方法、核酸の増幅を選択的に阻害する方法、およびこれらを実施するためのキット
JP7394301B2 (ja) * 2021-12-10 2023-12-08 日東紡績株式会社 二本鎖核酸の融解温度上昇化剤及びその用途

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US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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US5589335A (en) 1989-04-05 1996-12-31 Amoco Corporation Hybridization promotion reagents
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AU7634694A (en) * 1993-08-20 1995-03-21 University Of Medicine And Dentistry Of New Jersey Bridged polycationic polymer-oligonucleotide conjugates and methods for preparing same
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5731148A (en) * 1995-06-07 1998-03-24 Gen-Probe Incorporated Adduct protection assay
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WO1998027209A1 (en) 1996-12-18 1998-06-25 Emory University Polycationic oligomers
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
JP2001514859A (ja) * 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
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US6297016B1 (en) * 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
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US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
DE60228100D1 (de) * 2001-08-24 2008-09-18 Nof Corp Verfahren zur beurteilung einer fehlpaarung zwischen einzelsträngigen nukleinsäuremolekülen
JP5034027B2 (ja) * 2005-10-03 2012-09-26 株式会社Ｍｏｒｅｓｃｏ パーフルオロポリエーテル化合物、およびこれを用いた潤滑剤ならびに磁気ディスク

Also Published As

Publication number Publication date
AU2002230901B2 (en) 2006-09-07
US20040077014A1 (en) 2004-04-22
US7374945B2 (en) 2008-05-20
ATE447043T1 (de) 2009-11-15
WO2002048404A3 (en) 2003-10-23
US20020164614A1 (en) 2002-11-07
CA2436857A1 (en) 2002-06-20
JP2004537263A (ja) 2004-12-16
EP1377679A2 (de) 2004-01-07
WO2002048404A2 (en) 2002-06-20
EP1377679B1 (de) 2009-10-28
AU3090102A (en) 2002-06-24

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