BR112017015988A2 - método para a modificação dirigida a sítio de planta inteira através da expressão transiente de gene - Google Patents

método para a modificação dirigida a sítio de planta inteira através da expressão transiente de gene

Info

Publication number
BR112017015988A2
BR112017015988A2 BR112017015988-0A BR112017015988A BR112017015988A2 BR 112017015988 A2 BR112017015988 A2 BR 112017015988A2 BR 112017015988 A BR112017015988 A BR 112017015988A BR 112017015988 A2 BR112017015988 A2 BR 112017015988A2
Authority
BR
Brazil
Prior art keywords
plant
transient expression
whole plant
transformation method
modification
Prior art date
Application number
BR112017015988-0A
Other languages
English (en)
Chinese (zh)
Other versions
BR112017015988B1 (pt
Inventor
Gao Caixia
Zhang Yi
Wu Zhongyi
Zhang Kang
Original Assignee
Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences filed Critical Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
Publication of BR112017015988A2 publication Critical patent/BR112017015988A2/pt
Publication of BR112017015988B1 publication Critical patent/BR112017015988B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03048—Protein-tyrosine-phosphatase (3.1.3.48)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/10—Vectors comprising a non-peptidic targeting moiety

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a um método para realizar a modificação específica de sítio em uma planta inteira através da expressão transiente de gene, em que o método compreende as etapas a seguir: o emprego da planta inteira como um objeto de expressão transiente, a expressão transiente de uma nuclease distinta ao segmento de gene alvo na planta; sob a ação da nucleasse, o cisalhamento do segmento alvo, e a modalidade da modificação específica de sítio do segmento alvo por meio do reparo do dna da planta. também é provido um método para o cultivo de uma planta de mutação não transgênica mediante o uso do método acima, e a planta de mutação não transgênica obtida dessa maneira.
BR112017015988-0A 2015-01-27 2016-01-27 Métodos para a modificação sítio dirigida para um fragmento alvo de um gene alvo em uma planta inteira e para a obtenção de uma planta mutante livre de transgene BR112017015988B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201510040078.0 2015-01-27
CN201510040078 2015-01-27
PCT/CN2016/072352 WO2016119703A1 (zh) 2015-01-27 2016-01-27 一种通过基因瞬时表达对整株植物定点改造的方法

Publications (2)

Publication Number Publication Date
BR112017015988A2 true BR112017015988A2 (pt) 2018-03-20
BR112017015988B1 BR112017015988B1 (pt) 2024-02-27

Family

ID=56542435

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017015988-0A BR112017015988B1 (pt) 2015-01-27 2016-01-27 Métodos para a modificação sítio dirigida para um fragmento alvo de um gene alvo em uma planta inteira e para a obtenção de uma planta mutante livre de transgene

Country Status (13)

Country Link
US (2) US11421241B2 (pt)
EP (2) EP3252162B1 (pt)
JP (1) JP2018503392A (pt)
KR (1) KR102046450B1 (pt)
CN (1) CN105821073B (pt)
AR (1) AR103539A1 (pt)
AU (1) AU2016212529B2 (pt)
BR (1) BR112017015988B1 (pt)
CA (1) CA2973903C (pt)
DK (1) DK3252162T3 (pt)
EA (1) EA039511B1 (pt)
ES (1) ES2945315T3 (pt)
WO (1) WO2016119703A1 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
CN106834341B (zh) * 2016-12-30 2020-06-16 中国农业大学 一种基因定点突变载体及其构建方法和应用
JP2020072645A (ja) * 2017-01-31 2020-05-14 日本たばこ産業株式会社 植物に物質を導入する方法
CN107164402B (zh) * 2017-05-31 2020-10-16 未名兴旺系统作物设计前沿实验室（北京）有限公司 一种基于CRISPR-Cas9系统的基因编辑载体及其应用
CN107287230B (zh) * 2017-08-03 2020-10-13 沈阳农业大学 一种质粒载体及其构建方法
CA3083443A1 (en) 2017-11-27 2019-05-31 Riken Genome-edited plant production method
CN109929872A (zh) * 2017-12-18 2019-06-25 中国科学院遗传与发育生物学研究所 一种通过基因编辑技术创制番茄白果材料的方法
CN108374020A (zh) * 2017-12-28 2018-08-07 南京农业大学 一种改良的农杆菌介导大豆整体转化方法
US11566236B2 (en) * 2018-02-05 2023-01-31 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of hemoglobinopathies
WO2019205919A1 (zh) * 2018-04-28 2019-10-31 青岛清原化合物有限公司 一种生物突变体库的构建方法
CN108642078A (zh) * 2018-05-18 2018-10-12 江苏省农业科学院 基于CRISPR/Cas9基因编辑技术选育绿豆开花传粉突变体的方法及专用gRNA
CN108866096B (zh) * 2018-07-26 2021-11-16 山东农业大学 大田蔷薇属植株上建立病毒诱导基因沉默体系的方法
CN111254159B (zh) * 2018-11-30 2023-03-31 东北农业大学 一种大豆GmST1基因突变体植株及其制备方法
EP4110929A1 (en) * 2020-02-28 2023-01-04 KWS SAAT SE & Co. KGaA Immature inflorescence meristem editing
CN111647620A (zh) * 2020-06-10 2020-09-11 北京市农林科学院 一种菜薹非转基因突变株的创制方法
CN112385539A (zh) * 2020-11-05 2021-02-23 贵州师范大学 一种果壳不破裂早熟优质黑米荞的选育方法
CN112501179B (zh) * 2020-11-12 2022-05-24 福建农林大学 GmPIN1基因突变调控大豆结瘤和密植的方法及其应用
CN113717982B (zh) * 2021-08-30 2023-09-01 山东大学 一种利用基因编辑敲除籽粒发育相关蛋白TaGSR1增加小麦籽粒长度的方法
CN113846075A (zh) * 2021-11-29 2021-12-28 科稷达隆(北京)生物技术有限公司 Mad7-nls融合蛋白、用于植物基因组定点编辑的核酸构建物及其应用

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
NZ504300A (en) 1997-11-18 2002-06-28 Pioneer Hi Bred Int A method for directional stable transformation of eukaryotic cells using non-identical recombination sites
WO2000066746A1 (en) 1999-04-29 2000-11-09 Syngenta Limited Herbicide resistant plants
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
AU2001287862B2 (en) 2000-09-29 2006-12-14 Syngenta Limited Herbicide resistant plants
US20030135891A1 (en) 2001-12-04 2003-07-17 The Texas A&M University System Method of transforming intact plants
AU2003247962B2 (en) 2002-07-18 2008-06-12 Monsanto Technology Llc Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
JP5702144B2 (ja) 2007-09-27 2015-04-15 ダウ アグロサイエンシィズ エルエルシー ５−エノールピルビルシキミ酸−３−リン酸シンターゼ遺伝子をターゲッティングする改変ジンクフィンガータンパク質
JP2011120478A (ja) 2008-03-31 2011-06-23 Japan Tobacco Inc アグロバクテリウム菌による形質転換植物の作成方法
US10030245B2 (en) 2011-03-23 2018-07-24 E I Du Pont De Nemours And Company Methods for producing a complex transgenic trait locus
WO2013096567A2 (en) 2011-12-21 2013-06-27 Duke University The hsf-like transcription factor, tbf1, is a major molecular switch for growth-to-defense transition in plants
CN102558309B (zh) 2012-02-10 2014-09-17 浙江大学 一对转录激活子样效应因子核酸酶及其编码基因与应用
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
KR102091298B1 (ko) 2012-05-02 2020-03-19 다우 아그로사이언시즈 엘엘씨 말산 탈수소효소의 표적화된 변형
CN104471067B (zh) 2012-05-07 2020-08-14 桑格摩生物治疗股份有限公司 用于核酸酶介导的转基因靶向整合的方法和组合物
DK2800811T3 (en) 2012-05-25 2017-07-17 Univ Vienna METHODS AND COMPOSITIONS FOR RNA DIRECTIVE TARGET DNA MODIFICATION AND FOR RNA DIRECTIVE MODULATION OF TRANSCRIPTION
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
AU2014227831B2 (en) 2013-03-15 2020-01-30 Regents Of The University Of Minnesota Engineering plant genomes using CRISPR/Cas systems
WO2014194190A1 (en) 2013-05-30 2014-12-04 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
AU2014279694B2 (en) 2013-06-14 2020-07-23 Cellectis Methods for non-transgenic genome editing in plants
CN103382468B (zh) 2013-07-04 2015-04-29 中国科学院遗传与发育生物学研究所 一种水稻基因组定点改造方法
CN103343120B (zh) 2013-07-04 2015-03-04 中国科学院遗传与发育生物学研究所 一种小麦基因组定点改造方法
CN103555711A (zh) 2013-07-22 2014-02-05 安徽省农业科学院水稻研究所 一种主要农作物非转基因的基因组定向分子改良方法和应用
BR112016003591A8 (pt) 2013-08-22 2018-01-30 Du Pont promotor u6 de polimerase iii de soja e métodos de uso
BR102014027448A8 (pt) 2013-11-04 2021-08-24 Dow Agrosciences Llc cassete de doador de polinucleotídeo, uso de uma planta, de uma parte de planta, ou de uma célula vegetal compreendendo o referido cassete, e método para integração direcionada do mesmo
US10787684B2 (en) 2013-11-19 2020-09-29 President And Fellows Of Harvard College Large gene excision and insertion
CN103667338B (zh) 2013-11-28 2016-01-27 中国科学院遗传与发育生物学研究所 一种玉米基因组定点改造方法
EP3178935B1 (en) 2014-08-06 2022-12-21 Toolgen Incorporated Genome editing using campylobacter jejuni crispr/cas system-derived rgen
WO2016084084A1 (en) 2014-11-27 2016-06-02 Danziger Innovations Ltd. Nucleic acid constructs for genome editing
DE102015004187A1 (de) 2015-04-02 2016-10-06 Kws Saat Se Männlich sterile Pflanze der Gattung Triticum
CN106811479B (zh) 2015-11-30 2019-10-25 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用

Also Published As

Publication number Publication date
JP2018503392A (ja) 2018-02-08
EA201791681A1 (ru) 2018-03-30
KR20170098953A (ko) 2017-08-30
EP3252162B1 (en) 2023-03-01
EP4219730A1 (en) 2023-08-02
US20180016589A1 (en) 2018-01-18
KR102046450B1 (ko) 2019-11-19
EA039511B1 (ru) 2022-02-04
AU2016212529B2 (en) 2018-11-01
CN105821073B (zh) 2021-08-10
AR103539A1 (es) 2017-05-17
EP3252162A1 (en) 2017-12-06
ES2945315T3 (es) 2023-06-30
CA2973903A1 (en) 2016-08-04
DK3252162T3 (da) 2023-06-06
CA2973903C (en) 2024-03-12
CN105821073A (zh) 2016-08-03
EP3252162A4 (en) 2018-02-21
WO2016119703A1 (zh) 2016-08-04
US11421241B2 (en) 2022-08-23
BR112017015988B1 (pt) 2024-02-27
AU2016212529A1 (en) 2017-08-03
US20220411810A1 (en) 2022-12-29

Similar Documents

Publication Publication Date Title
BR112017015988A2 (pt) método para a modificação dirigida a sítio de planta inteira através da expressão transiente de gene
BR112018007070A2 (pt) método, composição e kit para a preparação de uma planta a partir de um protoplasto, planta regenerada e composição para clivagem de dna
BR112017018327A2 (pt) método para a expressão regulada de um complexo, planta ou célula vegetal, semente, método para modificação de uma sequência, método para alteração da expressão de pelo menos um polinucleotídeo
BR112019002455A2 (pt) método para edição de base em plantas
BR102014027438A8 (pt) Sequência recombinante, uso de uma planta, de uma parte de planta, ou de uma célula de planta de milho, método para preparação de uma célula vegetal transgênica, e sequências recombinante e genômica de milho
BR112017007083A2 (pt) moléculas de ácido nucleico subunidade copi coatomer delta que confere resistência a pragas de coleóptero e hemípteros
BR112016026136A2 (pt) composições e métodos para aumentar o desenvolvimento e o rendimento vegetal
BR112017008518A2 (pt) indutores de haploidia
BR102014027466A8 (pt) Sequência recombinante, uso de uma planta, uma parte de planta, ou uma célula de planta de soja, método para preparação de uma célula de planta transgênica, e loci genômicos de soja não gênicos purificados
BR112017017279A2 (pt) métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
BR112012030784A2 (pt) métodos de irrigação de planta com 1-mcp
CL2017003313A1 (es) Moléculas de adn con actividad reguladora de genes, célula de planta que las comprende, y método para controlar la expresión genética en plantas.
AR084774A1 (es) Moleculas de acido nucleico que confieren resistencia a plagas coleopteros
CL2018001546A1 (es) Formulaciones líquidas que contienen fungicidas
BR112014018986A8 (pt) Regulação do crescimento de plantas
BR112015021210B8 (pt) Método para reduzir a expressão de um ácido nucleico alvo em uma planta e ácido nucleico recombinante
BR112018069506A2 (pt) métodos para transfectar plantas e para reduzir eventos de integração aleatória
BR112019005103A2 (pt) método para determinar a quantidade requerida por plantas de cultura em um campo, método para tratar plantas de cultura em um campo, sistema e produto de programa de computador
BR112016007902A2 (pt) rnas não codificantes regulatórios como determinantes de macho-esterilidade em gramas e outras plantas monocotiledôneas
UY35865A (es) ?métodos para aumentar la resistencia de las plantas al estrés abiótico?.
BR112017005441A2 (pt) método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método.
CO2017007985A2 (es) Genes de acetohidroxiácido sintasa mutados en euphorbiaceae y material vegetal que comprende dichos genes
BR112017011246A2 (pt) composições e métodos para introdução de micronutriente
UY36797A (es) Hidrazidas de ácido carboxílico heteroarilo sustituidas o sus sales y su uso para aumentar la tolerancia al estrés en plantas
BR112016016772A2 (pt) Método para obter células vegetais transformadas

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 27/01/2016, OBSERVADAS AS CONDICOES LEGAIS