BR112017005441A2 - método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método. - Google Patents

método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método.

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Publication number
BR112017005441A2
BR112017005441A2 BR112017005441A BR112017005441A BR112017005441A2 BR 112017005441 A2 BR112017005441 A2 BR 112017005441A2 BR 112017005441 A BR112017005441 A BR 112017005441A BR 112017005441 A BR112017005441 A BR 112017005441A BR 112017005441 A2 BR112017005441 A2 BR 112017005441A2
Authority
BR
Brazil
Prior art keywords
transgenic
plant
tissue
cell
agrobacterium
Prior art date
Application number
BR112017005441A
Other languages
English (en)
Other versions
BR112017005441B1 (pt
Inventor
Faion-Molina Mayra
Cezar De Lucca Paulo
Original Assignee
CTC Centro de Tecnologia Canavieira S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CTC Centro de Tecnologia Canavieira S A filed Critical CTC Centro de Tecnologia Canavieira S A
Publication of BR112017005441A2 publication Critical patent/BR112017005441A2/pt
Publication of BR112017005441B1 publication Critical patent/BR112017005441B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

Abstract

são providos composições e métodos para transformação de plantas, preferencialmente monocotiledôneas, e ainda mais preferencialmente, cana-de-açúcar. os métodos de transformação envolvem infecção de tecido vegetal com agrobacterium, e co-cultivo utilizando meio de cultura compreendendo altas concentrações de agente gelificante, com o resultado de inibir o crescimento exacerbado da bactéria e aumentar as frequências de transformação. a invenção inclui regenerar plantas transformadas, e as plantas transformadas em si.
BR112017005441-8A 2014-09-26 2015-09-16 Método para transformar uma célula de planta ou tecido de planta usando agrobacterium, meio de cultura, e, uso de um método BR112017005441B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
BRBR1020140239308 2014-09-26
BR102014023930A BR102014023930A2 (pt) 2014-09-26 2014-09-26 método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método para transformar uma célula de planta ou tecido de planta
PCT/BR2015/050154 WO2016044909A1 (pt) 2014-09-26 2015-09-16 Metodo para transformar uma celula de planta ou tecido de planta usando agrobacterium, planta transgenica, celula transgenica ou tecido transgenico, meio de cultura, e, uso de um metodo para transformar uma celula de planta ou tecido de planta.

Publications (2)

Publication Number Publication Date
BR112017005441A2 true BR112017005441A2 (pt) 2017-12-12
BR112017005441B1 BR112017005441B1 (pt) 2023-03-21

Family

ID=55579978

Family Applications (2)

Application Number Title Priority Date Filing Date
BR102014023930A BR102014023930A2 (pt) 2014-09-26 2014-09-26 método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método para transformar uma célula de planta ou tecido de planta
BR112017005441-8A BR112017005441B1 (pt) 2014-09-26 2015-09-16 Método para transformar uma célula de planta ou tecido de planta usando agrobacterium, meio de cultura, e, uso de um método

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR102014023930A BR102014023930A2 (pt) 2014-09-26 2014-09-26 método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método para transformar uma célula de planta ou tecido de planta

Country Status (5)

Country Link
US (1) US10774335B2 (pt)
CN (1) CN107002030B (pt)
AU (1) AU2015321438B8 (pt)
BR (2) BR102014023930A2 (pt)
WO (1) WO2016044909A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6971478B2 (ja) * 2016-12-27 2021-11-24 国立研究開発法人農業・食品産業技術総合研究機構 ゲノム編集植物の作出方法
CN110904144B (zh) * 2019-09-18 2022-03-22 四川农业大学 一种利用发根农杆菌诱导产生鸭茅毛状根的方法
CN114303948B (zh) * 2021-12-29 2022-11-11 北京市农林科学院 一种帚状须芒草愈伤组织诱导培养基及帚状须芒草再生体系的建立方法
CN114831023B (zh) * 2022-03-28 2023-04-07 浙江省农业科学院 一种以花梗为外植体的花椰菜高效遗传转化方法
CN116042694A (zh) * 2022-11-24 2023-05-02 中国科学院南京土壤研究所 禾本科狼尾草属植物非组培遗传转化方法

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US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
TW261517B (pt) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
WO1994000977A1 (en) 1992-07-07 1994-01-20 Japan Tobacco Inc. Method of transforming monocotyledon
CA2140910C (en) 1992-07-27 1999-03-23 Jeffrey A. Townsend An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
MX257801B (en) 1997-06-02 2008-06-10 Syngenta Participations Ag Plant transformation methods
EP1009844B1 (en) 1997-08-25 2004-06-16 Nunhems Zaden Bv IMPROVED $i(AGROBACTERIUM)-MEDIATED TRANSFORMATION OF PLANTS
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
AU2267201A (en) 1999-12-15 2001-06-25 Paula Olhoft Method to enhance agrobacterium-mediated transformation of plants
ATE509946T1 (de) 2002-11-12 2011-06-15 Zakrytoe Aktsionernoe Obschestvo S Evrogens Fluoreszenzproteine und chromoproteine aus nicht- aequorea-hydrozoa-spezies sowie verfahren zur verwendung davon
CN102639703A (zh) 2009-06-25 2012-08-15 先正达参股股份有限公司 土壤杆菌属(agrobacterium)介导甘蔗转化的方法
CN101768604B (zh) * 2010-01-19 2012-05-23 中国热带农业科学院热带生物技术研究所 一种高效快速的甘蔗转基因方法
CA2801348A1 (en) 2010-06-24 2011-12-29 Syngenta Participations Ag Methods for agrobacterium-mediated transformation of sugar cane
BR112014004812A2 (pt) * 2011-08-31 2018-10-23 Du Pont métodos para regenerar uma planta e para produzir uma planta transformada

Also Published As

Publication number Publication date
US10774335B2 (en) 2020-09-15
BR102014023930A2 (pt) 2016-05-17
BR112017005441B1 (pt) 2023-03-21
US20170298371A1 (en) 2017-10-19
AU2015321438A1 (en) 2017-04-13
AU2015321438B8 (en) 2021-12-02
CN107002030B (zh) 2021-05-25
WO2016044909A1 (pt) 2016-03-31
AU2015321438A8 (en) 2021-12-02
AU2015321438B2 (en) 2021-11-18
CN107002030A (zh) 2017-08-01

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