BR112018004108A2 - ochrobactrum haywardense h1 isolada, ochrobactrum haywardense h1, método para produzir uma célula vegetal transformada, kit e vetor - Google Patents

ochrobactrum haywardense h1 isolada, ochrobactrum haywardense h1, método para produzir uma célula vegetal transformada, kit e vetor

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Publication number
BR112018004108A2
BR112018004108A2 BR112018004108A BR112018004108A BR112018004108A2 BR 112018004108 A2 BR112018004108 A2 BR 112018004108A2 BR 112018004108 A BR112018004108 A BR 112018004108A BR 112018004108 A BR112018004108 A BR 112018004108A BR 112018004108 A2 BR112018004108 A2 BR 112018004108A2
Authority
BR
Brazil
Prior art keywords
ochrobactrum
plant cell
methods
hawardward
kit
Prior art date
Application number
BR112018004108A
Other languages
English (en)
Inventor
Anand Ajith
J Kinney Anthony
Ann Marie Rosen Barabara
Cho Hyeon-Je
Wei Jun-Zhi
E Mcbride Kevin
Lassner Michael
M Bertain Sean
Henry Bass Steven
M Klein Theodore
Moy York
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of BR112018004108A2 publication Critical patent/BR112018004108A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205Agrobacterium mediated transformation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Abstract

trata-se de métodos e composições para transformação de plantas mediada por ochrobactrum. os métodos incluem, porém sem limitação, o uso de uma cepa de ochrobactrum para transferir um polinucleotídeo de interesse para uma célula vegetal. os mesmos incluem métodos dependentes de vird2. as composições incluem uma cepa de ochrobactrum, dnas de transferência, construtos e/ou plasmídeos. as mesmas incluem cepas de ochrobactrum que têm um plasmídeo que compreende um ou mais dentre genes de virulência, região de limite e/ou origem de replicação. células vegetais, tecidos, plantas e sementes que compreendem um polinucleotídeo de interesse produzido pelos métodos também são fornecidos.
BR112018004108A 2015-08-28 2016-08-26 ochrobactrum haywardense h1 isolada, ochrobactrum haywardense h1, método para produzir uma célula vegetal transformada, kit e vetor BR112018004108A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562211267P 2015-08-28 2015-08-28
PCT/US2016/049135 WO2017040343A1 (en) 2015-08-28 2016-08-26 Ochrobactrum-mediated transformation of plants

Publications (1)

Publication Number Publication Date
BR112018004108A2 true BR112018004108A2 (pt) 2018-12-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018004108A BR112018004108A2 (pt) 2015-08-28 2016-08-26 ochrobactrum haywardense h1 isolada, ochrobactrum haywardense h1, método para produzir uma célula vegetal transformada, kit e vetor

Country Status (10)

Country Link
US (1) US11236347B2 (pt)
EP (1) EP3341483B1 (pt)
JP (1) JP2018527931A (pt)
CN (1) CN108513584A (pt)
AU (1) AU2016315655A1 (pt)
BR (1) BR112018004108A2 (pt)
CA (1) CA2992488A1 (pt)
DK (1) DK3341483T3 (pt)
ES (1) ES2773072T3 (pt)
WO (1) WO2017040343A1 (pt)

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BR112021020770A2 (pt) 2019-04-18 2022-01-04 Pioneer Hi Bred Int Fatores de embriogênese para reprogramação celular de uma célula vegetal
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