BR112019023377A2 - Criação de um gene resistente a herbicida e uso do mesmo - Google Patents

Criação de um gene resistente a herbicida e uso do mesmo Download PDF

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Publication number
BR112019023377A2
BR112019023377A2 BR112019023377-6A BR112019023377A BR112019023377A2 BR 112019023377 A2 BR112019023377 A2 BR 112019023377A2 BR 112019023377 A BR112019023377 A BR 112019023377A BR 112019023377 A2 BR112019023377 A2 BR 112019023377A2
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Brazil
Prior art keywords
creating
same
resistant gene
herbicide resistant
herbicide
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BR112019023377-6A
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English (en)
Inventor
Gao Caixia
Jiang Linjian
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Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
China Agricultural University
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Application filed by Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences, China Agricultural University filed Critical Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences
Publication of BR112019023377A2 publication Critical patent/BR112019023377A2/pt

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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Abstract

a presente invenção refere-se a um método para a criação de uma planta resistente ao herbicida pelo sistema crispr/cas9. um método de rastreamento de sítios de mutação de gene endógeno capazes de conferir resistência ao herbicida em plantas e o uso de sítios de mutação de gene endógeno identificados para a reprodução de culturas também são fornecidos.
BR112019023377-6A 2017-05-11 2018-05-11 Criação de um gene resistente a herbicida e uso do mesmo BR112019023377A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201710329242 2017-05-11
CN201710329242.9 2017-05-11
PCT/CN2018/086501 WO2018205995A1 (en) 2017-05-11 2018-05-11 Creation of herbicide resistant gene and use thereof

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BR112019023377A2 true BR112019023377A2 (pt) 2020-06-16

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US (2) US11345924B2 (pt)
EP (1) EP3622061A4 (pt)
JP (1) JP2020519310A (pt)
KR (1) KR20200008137A (pt)
CN (2) CN108866092B (pt)
AR (1) AR111790A1 (pt)
AU (1) AU2018264457A1 (pt)
BR (1) BR112019023377A2 (pt)
CA (1) CA3062976A1 (pt)
EA (1) EA201992637A1 (pt)
WO (1) WO2018205995A1 (pt)

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WO2019233349A1 (zh) * 2018-06-04 2019-12-12 青岛清原化合物有限公司 突变型对羟苯基丙酮酸双氧化酶、其编码核酸以及应用
CN111139261B (zh) * 2019-02-28 2022-05-31 山东省农业科学院作物研究所 一种利用基因编辑降低小麦籽粒多酚氧化酶含量的方法
CN110373414A (zh) * 2019-08-01 2019-10-25 中国农业科学院作物科学研究所 一种高效创制磺酰脲类除草剂抗性玉米的方法及其应用
BR112022004586A2 (pt) * 2019-09-12 2022-06-14 Inst Genetics & Developmental Biology Cas Plantas resistentes a herbicidas
MX2022001245A (es) * 2019-11-07 2022-02-22 Qingdao Kingagroot Chemical Compound Co Ltd Un metodo para generar una nueva mutacion en un organismo y su uso.
CN112813064A (zh) * 2019-11-18 2021-05-18 安徽省农业科学院水稻研究所 一种创制高抗稳定的内源抗除草剂水稻的方法
CN110982818B (zh) * 2019-12-20 2022-03-08 北京市农林科学院 核定位信号f4nls在高效创制水稻除草剂抗性材料中的应用
CN111423990B (zh) * 2020-04-10 2021-08-27 科稷达隆(北京)生物技术有限公司 一种乙氧氟草醚敏感型酵母菌及其制备方法
CN111560396B (zh) * 2020-05-07 2022-03-25 海南波莲水稻基因科技有限公司 植物转基因筛选载体pCALSm1及其应用
CN113755457B (zh) * 2020-09-23 2022-11-22 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
AR124409A1 (es) * 2020-12-17 2023-03-22 Suzhou Qi Biodesign Biotechnology Company Ltd Plantas resistentes a herbicidas
WO2022151352A1 (zh) * 2021-01-15 2022-07-21 江苏省农业科学院 具有除草剂抗性的hppd突变型蛋白及其应用
CN112813094B (zh) * 2021-02-08 2023-03-24 山东师范大学 一种抗二硝基苯胺类除草剂小麦的构建方法
CN113265401B (zh) * 2021-04-15 2022-06-03 江苏里下河地区农业科学研究所 一种通过基因编辑提高水稻对HPPD抑制剂类除草剂抗性的方法及其专用sgRNA
CN113201557B (zh) * 2021-05-10 2023-04-07 安徽省农业科学院水稻研究所 一种引导编辑系统介导作物产生内源除草剂抗性的方法
KR20230139064A (ko) * 2022-03-25 2023-10-05 기초과학연구원 식물세포 소기관 dna의 아데닌을 구아닌으로 염기 교정

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6919619B2 (en) 2003-02-28 2005-07-19 The Regents Of The University Of Michigan Actively-shielded signal wires
WO2007149069A2 (en) * 2006-06-15 2007-12-27 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Resistance to acetolactate synthase-inhibiting herbicides
TW201113376A (en) 2009-09-01 2011-04-16 Basf Agrochemical Products Bv Herbicide-tolerant plants
US9121022B2 (en) 2010-03-08 2015-09-01 Monsanto Technology Llc Method for controlling herbicide-resistant plants
BR112013019510B1 (pt) * 2011-02-01 2021-09-21 Colorado Wheat Research Foundation, Inc Método para obtenção de uma planta de trigo, método para cultivar uma planta de trigo, método para produzir uma planta de trigo, método para identificar uma planta de trigo que é resistente ao herbicida acetil-coa carboxilase, método para conferir resistência a herbicidas accase a uma planta, polinucleotídeo isolado, construto de ácido nucléico, cassete de expressão, método para obtenção de uma planta transgênica e polipeptídeo isolado com atividade accase
PT2788482T (pt) * 2011-11-14 2019-07-23 Basf Se Métodos e composições para isolar, identificar e caracterizar mutações tolerantes a herbicidas de accase plastídica monocotiledónea com utilização de um sistema de modelo
EP2981166B1 (en) * 2013-04-05 2020-09-09 Dow AgroSciences LLC Methods and compositions for integration of an exogenous sequence within the genome of plants
WO2014186686A2 (en) * 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
EP3036331B1 (en) * 2013-08-21 2021-06-16 BASF Agricultural Solutions Seed US LLC Als inhibitor herbicide tolerant mutant plants
EP3611268A1 (en) * 2013-08-22 2020-02-19 E. I. du Pont de Nemours and Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
EP3115457B1 (en) * 2014-03-05 2019-10-02 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
DK3132034T3 (da) 2014-04-14 2020-10-19 Nemesis Bioscience Ltd Terapeutikum
KR102127418B1 (ko) * 2015-08-14 2020-06-26 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 부위-특이적인 뉴클레오티드 치환을 통해 글리포세이트-내성 벼를 수득하는 방법
US20180273960A1 (en) 2015-10-20 2018-09-27 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
AR110075A1 (es) * 2016-11-14 2019-02-20 Inst Genetics & Developmental Biology Cas Un método para edición basal en plantas

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