MX2022001245A - Un metodo para generar una nueva mutacion en un organismo y su uso. - Google Patents

Un metodo para generar una nueva mutacion en un organismo y su uso.

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Publication number
MX2022001245A
MX2022001245A MX2022001245A MX2022001245A MX2022001245A MX 2022001245 A MX2022001245 A MX 2022001245A MX 2022001245 A MX2022001245 A MX 2022001245A MX 2022001245 A MX2022001245 A MX 2022001245A MX 2022001245 A MX2022001245 A MX 2022001245A
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MX
Mexico
Prior art keywords
organisms
application
produced
generating new
new mutations
Prior art date
Application number
MX2022001245A
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English (en)
Inventor
Huarong Li
Wei Qi
Bo Chen
Sudong Mo
Linjian Jiang
Jiyao Wang
Yucai Li
Original Assignee
Qingdao Kingagroot Chemical Compound Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Qingdao Kingagroot Chemical Compound Co Ltd filed Critical Qingdao Kingagroot Chemical Compound Co Ltd
Publication of MX2022001245A publication Critical patent/MX2022001245A/es

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Abstract

La presente invención pertenece al campo técnico de la ingeniería genética y, de manera específica, se refiere a un método para generar una mutación específica para un sitio en un organismo en ausencia de una plantilla de ADN artificial y un uso de ella. El método comprende los siguientes pasos: generar de manera secuencial dos o más roturas de ADN en un sitio específico en un genoma de un organismo y repararlas en forma espontánea respectivamente, en donde una rotura de ADN posterior se genera sobre la base de una nueva secuencia generada a partir de una reparación de una rotura de ADN previa. En la presente invención, una nueva diana se diseña sobre la base de una secuencia formada mediante un nuevo evento de reparación generada por edición secuencial y, de esta forma, se pueden formar mutaciones secuencialmente en muchas ocasiones en un sitio específico en el genoma, lo cual enriquece en gran medida los tipos de eventos de reparación después de roturas de ADN, y realiza nuevas mutaciones por sustitución, eliminación e inserción de bases que no pueden obtenerse en una única edición genética.
MX2022001245A 2019-11-07 2020-10-13 Un metodo para generar una nueva mutacion en un organismo y su uso. MX2022001245A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN201911081617 2019-11-07
CN202010821877 2020-08-15
CN202010974151 2020-09-16
PCT/CN2020/120633 WO2021088601A1 (zh) 2019-11-07 2020-10-13 一种在生物体内产生新突变的方法及应用

Publications (1)

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MX2022001245A true MX2022001245A (es) 2022-02-22

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MX2022001245A MX2022001245A (es) 2019-11-07 2020-10-13 Un metodo para generar una nueva mutacion en un organismo y su uso.

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US (1) US20230287389A1 (es)
EP (1) EP3889266A4 (es)
JP (1) JP2023500357A (es)
KR (1) KR20220093324A (es)
CN (1) CN112251419B (es)
AU (1) AU2020378387A1 (es)
BR (1) BR112022008916A2 (es)
CA (1) CA3159682A1 (es)
CL (1) CL2021003593A1 (es)
IL (1) IL289541A (es)
MX (1) MX2022001245A (es)
WO (1) WO2021088601A1 (es)
ZA (1) ZA202203920B (es)

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CN113846075A (zh) * 2021-11-29 2021-12-28 科稷达隆(北京)生物技术有限公司 Mad7-nls融合蛋白、用于植物基因组定点编辑的核酸构建物及其应用
CN114145231B (zh) * 2021-12-16 2022-09-13 广西壮族自治区中国科学院广西植物研究所 一种二色波罗蜜种苗快速繁殖方法
CN115644067B (zh) * 2022-12-09 2023-03-14 云南省农业科学院药用植物研究所 一种生产滇黄精双单倍体的方法
CN116286869B (zh) * 2023-03-23 2024-04-05 石河子大学 一种羽毛针禾糖转运蛋白基因SpSWEET14在提高植物抗寒性中的应用

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CA3159682A1 (en) 2021-05-14
BR112022008916A2 (pt) 2022-08-02
WO2021088601A1 (zh) 2021-05-14
TW202130813A (zh) 2021-08-16
ZA202203920B (en) 2023-11-29
AU2020378387A1 (en) 2022-01-20
JP2023500357A (ja) 2023-01-05
US20230287389A1 (en) 2023-09-14
CL2021003593A1 (es) 2022-08-19
KR20220093324A (ko) 2022-07-05
CN112251419A (zh) 2021-01-22
CN112251419B (zh) 2023-11-10
EP3889266A1 (en) 2021-10-06
IL289541A (en) 2022-03-01
EP3889266A4 (en) 2022-08-10

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