CL2021003593A1 - Un método para generar una nueva mutación en un organismo y sus usos. - Google Patents
Un método para generar una nueva mutación en un organismo y sus usos.Info
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- CL2021003593A1 CL2021003593A1 CL2021003593A CL2021003593A CL2021003593A1 CL 2021003593 A1 CL2021003593 A1 CL 2021003593A1 CL 2021003593 A CL2021003593 A CL 2021003593A CL 2021003593 A CL2021003593 A CL 2021003593A CL 2021003593 A1 CL2021003593 A1 CL 2021003593A1
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- new
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- generating
- dna
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- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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Abstract
La presente invención pertenece al campo técnico de la ingeniería genética y, de manera específica, se refiere a un método para generar una mutación específica para un sitio en un organismo en ausencia de una plantilla de ADN artificial y un uso de ella. El método comprende los siguientes pasos: generar de manera secuencial dos o más roturas de ADN en un sitio específico en un genoma de un organismo y repararlas en forma espontánea respectivamente, en donde una rotura de ADN posterior se genera sobre la base de una nueva secuencia generada a partir de una reparación de una rotura de ADN previa. En la presente invención, una nueva diana se diseña sobre la base de una secuencia formada mediante un nuevo evento de reparación generada por edición secuencial y, de esta forma, se pueden formar mutaciones secuencialmente en muchas ocasiones en un sitio específico en el genoma, lo cual enriquece en gran medida los tipos de eventos de reparación después de roturas de ADN, y realiza nuevas mutaciones por sustitución, eliminación e inserción de bases que no pueden obtenerse en una única edición genética.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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CN201911081617 | 2019-11-07 | ||
CN202010821877 | 2020-08-15 | ||
CN202010974151 | 2020-09-16 |
Publications (1)
Publication Number | Publication Date |
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CL2021003593A1 true CL2021003593A1 (es) | 2022-08-19 |
Family
ID=74243079
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2021003593A CL2021003593A1 (es) | 2019-11-07 | 2021-12-30 | Un método para generar una nueva mutación en un organismo y sus usos. |
Country Status (13)
Country | Link |
---|---|
US (1) | US20230287389A1 (es) |
EP (1) | EP3889266A4 (es) |
JP (1) | JP2023500357A (es) |
KR (1) | KR20220093324A (es) |
CN (1) | CN112251419B (es) |
AU (1) | AU2020378387A1 (es) |
BR (1) | BR112022008916A2 (es) |
CA (1) | CA3159682A1 (es) |
CL (1) | CL2021003593A1 (es) |
IL (1) | IL289541A (es) |
MX (1) | MX2022001245A (es) |
WO (1) | WO2021088601A1 (es) |
ZA (1) | ZA202203920B (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115161316A (zh) * | 2021-04-02 | 2022-10-11 | 上海科技大学 | 一种引导编辑工具、融合rna及其用途 |
CN113846075A (zh) * | 2021-11-29 | 2021-12-28 | 科稷达隆(北京)生物技术有限公司 | Mad7-nls融合蛋白、用于植物基因组定点编辑的核酸构建物及其应用 |
CN114145231B (zh) * | 2021-12-16 | 2022-09-13 | 广西壮族自治区中国科学院广西植物研究所 | 一种二色波罗蜜种苗快速繁殖方法 |
CN117987447A (zh) * | 2022-11-02 | 2024-05-07 | 广州大学 | 一种真核细胞持续进化的控制方法及其应用 |
CN115644067B (zh) * | 2022-12-09 | 2023-03-14 | 云南省农业科学院药用植物研究所 | 一种生产滇黄精双单倍体的方法 |
CN116286869B (zh) * | 2023-03-23 | 2024-04-05 | 石河子大学 | 一种羽毛针禾糖转运蛋白基因SpSWEET14在提高植物抗寒性中的应用 |
CN116410989B (zh) * | 2023-05-12 | 2024-06-14 | 云南农业大学 | 一种病毒诱导的三七pds基因沉默体系及应用 |
CN117918260B (zh) * | 2024-03-21 | 2024-05-24 | 西南林业大学 | 一种云南松组培苗的繁殖方法 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104017817B (zh) * | 2007-04-04 | 2017-12-01 | 巴斯福植物科学有限公司 | Ahas突变体 |
WO2010039750A2 (en) * | 2008-10-01 | 2010-04-08 | Monsanto Technology Llc | Transgenic plants with enhanced agronomic traits |
EP3036331B1 (en) * | 2013-08-21 | 2021-06-16 | BASF Agricultural Solutions Seed US LLC | Als inhibitor herbicide tolerant mutant plants |
US20160298129A1 (en) * | 2013-12-02 | 2016-10-13 | Bayer Cropscience Nv | Als inhibitor herbicide tolerant mutant plants |
MX2018005377A (es) * | 2015-11-30 | 2018-11-09 | Univ Duke | Dianas terapeuticas para la correccion del gen de la distrofina humana por edicion genica y metodos de uso. |
US20170314033A1 (en) * | 2016-05-02 | 2017-11-02 | Ohio State Innovation Foundation | Herbicide-resistant taraxacum kok-saghyz and taraxacum brevicorniculatum |
EP3573448A4 (en) * | 2017-01-28 | 2020-09-02 | Inari Agriculture, Inc. | NEW PLANT CELLS, PLANTS AND SEEDS |
US20210230616A1 (en) * | 2017-05-05 | 2021-07-29 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Methods for isolating cells without the use of transgenic marker sequences |
WO2018202199A1 (en) * | 2017-05-05 | 2018-11-08 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Methods for isolating cells without the use of transgenic marker sequences |
EP3622061A4 (en) * | 2017-05-11 | 2021-01-27 | Institute Of Genetics And Developmental Biology Chinese Academy of Sciences | CREATION OF A HERBICIDE RESISTANT GENE AND USE OF IT |
CN110157727A (zh) * | 2017-12-21 | 2019-08-23 | 中国科学院遗传与发育生物学研究所 | 植物碱基编辑方法 |
CN111699254A (zh) * | 2018-02-08 | 2020-09-22 | 齐默尔根公司 | 使用crispr在棒状杆菌属中进行基因组编辑 |
WO2019196717A1 (zh) * | 2018-04-13 | 2019-10-17 | 青岛清原化合物有限公司 | 一种随机突变的基因编辑系统及其应用 |
US20190330643A1 (en) * | 2018-04-25 | 2019-10-31 | The Catholic University Of America | Engineering of bacteriophages by genome editing using the crispr-cas9 system |
CN109536527A (zh) * | 2018-11-27 | 2019-03-29 | 中国科学院动物研究所 | 一种基因点突变修复的新方法 |
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- 2020-10-13 CN CN202011089074.9A patent/CN112251419B/zh active Active
- 2020-10-13 WO PCT/CN2020/120633 patent/WO2021088601A1/zh unknown
- 2020-10-13 MX MX2022001245A patent/MX2022001245A/es unknown
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2021
- 2021-12-30 CL CL2021003593A patent/CL2021003593A1/es unknown
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2022
- 2022-01-02 IL IL289541A patent/IL289541A/en unknown
- 2022-04-06 ZA ZA2022/03920A patent/ZA202203920B/en unknown
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TW202130813A (zh) | 2021-08-16 |
US20230287389A1 (en) | 2023-09-14 |
MX2022001245A (es) | 2022-02-22 |
EP3889266A1 (en) | 2021-10-06 |
AU2020378387A1 (en) | 2022-01-20 |
IL289541A (en) | 2022-03-01 |
BR112022008916A2 (pt) | 2022-08-02 |
KR20220093324A (ko) | 2022-07-05 |
JP2023500357A (ja) | 2023-01-05 |
CN112251419B (zh) | 2023-11-10 |
CN112251419A (zh) | 2021-01-22 |
ZA202203920B (en) | 2023-11-29 |
EP3889266A4 (en) | 2022-08-10 |
CA3159682A1 (en) | 2021-05-14 |
WO2021088601A1 (zh) | 2021-05-14 |
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